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Q08CK7 (IF2B1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor 2 mRNA-binding protein 1

Short name=IGF2 mRNA-binding protein 1
Short name=IMP-1
Alternative name(s):
IGF-II mRNA-binding protein 1
VICKZ family member 1
Gene names
Name:igf2bp1
Synonyms:vickz1
ORF Names:zgc:152963
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Plays a direct role in the transport and translation of transcripts required for axonal regeneration in adult sensory neurons By similarity. Regulates localized beta-actin/ACTB mRNA translation in polarized cells, a crucial process for cell migration and neurite outgrowth. Promotes the directed movement of cells by fine-tuning intracellular signaling networks and enhances the velocity of cell migration By similarity.

Subunit structure

Component of the CRD-mediated complex By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Cell projectiongrowth cone By similarity. Cell projectionfilopodium By similarity. Cell projectionlamellipodium By similarity.

Domain

Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute to transcript binding. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for localization to stress granules. KH3 and KH4 mediate association with the cytoskeleton. Two nuclear export signals (NES) have been identified in KH2 and KH4 domains, respectively. Only KH2 NES is XPO1-dependent. Both NES may be redundant By similarity.

Sequence similarities

Belongs to the RRM IMP/VICKZ family.

Contains 4 KH domains.

Contains 2 RRM (RNA recognition motif) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 598598Insulin-like growth factor 2 mRNA-binding protein 1
PRO_0000282537

Regions

Domain2 – 7574RRM 1
Domain81 – 15676RRM 2
Domain198 – 26366KH 1
Domain279 – 34668KH 2
Domain407 – 47266KH 3
Domain489 – 55567KH 4

Amino acid modifications

Modified residue1851Phosphoserine Ref.3
Modified residue3991Phosphotyrosine By similarity
Modified residue5731Phosphothreonine Ref.3
Modified residue5831Phosphothreonine Ref.3
Modified residue5871Phosphoserine Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q08CK7 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: CED67F6C29CA2AAD

FASTA59865,530
        10         20         30         40         50         60 
MNKLYIGNLN EKVTAEDLVK TFEDYKIPYS GQFLMKTGYA SVDCPDDQWA MKAIETFSGK 

        70         80         90        100        110        120 
VELHGKRIEV EHSVPKKQRT RKLQIRNIPP HLQWEVLDGL LAQYGTVENC EQVNTDSETA 

       130        140        150        160        170        180 
VVNVTYGTRE QARQAIQKLN GYQFDNNALR VSYIPDENSE VDSQRGPDNG RRPGYGPRGT 

       190        200        210        220        230        240 
SRQMSPGSGI PSKHQHADIP LRLLVPTQYV GAIIGKEGAT IRNITKQTQS KIDVHRKENA 

       250        260        270        280        290        300 
GAAEKPISIH STPEGCSAAC RMILEIMNQE AKDTKTADEV PLKVLAHNNF VGRLIGKEGR 

       310        320        330        340        350        360 
NLKKVEQDTD TKITISPLQD LTLYNPERTI TVKGSIEACC LAEQEIMKKV REAYDNDIAA 

       370        380        390        400        410        420 
MNQQTHLIPG LNLGAIGLFP PSSAMPPPAL GNSVPGPPYG PMGASEQETV HVYIPAQAVG 

       430        440        450        460        470        480 
ALIGKKGQHI KQLSRFAGAS IKIAPAEAPD SKMRMVIVTG PPEAQFKAQG RIYGKLKEEN 

       490        500        510        520        530        540 
FFGPKEEVKL ETHIKVAAAA AGRVIGKGGK TVNELQNLTA AEVVVPREQT PDEHDQVIVK 

       550        560        570        580        590 
IIGHFYASQL AQRKIRDILT QVKQQQKGGG MGTPQGPHPQ GMTELGSPQG LAQEPRRK 

« Hide

References

« Hide 'large scale' references
[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"VICKZ proteins: a multi-talented family of regulatory RNA-binding proteins."
Yisraeli J.K.
Biol. Cell 97:87-96(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[3]"Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo."
Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R.
J. Proteome Res. 7:1555-1564(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185; THR-573; THR-583 AND SER-587, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC124196 mRNA. Translation: AAI24197.1.
RefSeqNP_001070027.1. NM_001076559.1.
UniGeneDr.81351.

3D structure databases

ProteinModelPortalQ08CK7.
SMRQ08CK7. Positions 1-157.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000082159.

Proteomic databases

PRIDEQ08CK7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID558182.
KEGGdre:558182.

Organism-specific databases

CTD10642.
ZFINZDB-GENE-060929-1258. igf2bp1.

Phylogenomic databases

eggNOGNOG249985.
HOGENOMHOG000000675.
HOVERGENHBG052725.
InParanoidQ08CK7.
KOK17391.
OrthoDBEOG7T7GSK.
PhylomeDBQ08CK7.

Family and domain databases

Gene3D3.30.1370.10. 4 hits.
3.30.70.330. 2 hits.
InterProIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00013. KH_1. 4 hits.
[Graphical view]
SMARTSM00322. KH. 4 hits.
SM00360. RRM. 2 hits.
[Graphical view]
SUPFAMSSF54791. SSF54791. 4 hits.
PROSITEPS50084. KH_TYPE_1. 4 hits.
PS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20882359.
PROQ08CK7.

Entry information

Entry nameIF2B1_DANRE
AccessionPrimary (citable) accession number: Q08CK7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 31, 2006
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families