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Q08BW0

- OTU5A_DANRE

UniProt

Q08BW0 - OTU5A_DANRE

Protein

OTU domain-containing protein 5-A

Gene

otud5a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (31 Oct 2006)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei219 – 2191Sequence Analysis
    Active sitei222 – 2221NucleophileBy similarity
    Active sitei327 – 3271By similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. protein K48-linked deubiquitination Source: UniProtKB
    2. protein K63-linked deubiquitination Source: UniProtKB
    3. response to lipopolysaccharide Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_216466. Negative regulators of RIG-I/MDA5 signaling.

    Protein family/group databases

    MEROPSiC85.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    OTU domain-containing protein 5-A (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme A
    Short name:
    DUBA
    Gene namesi
    Name:otud5a
    Synonyms:otud5
    ORF Names:si:dkey-83k24.3, si:rp71-30i22.1, zgc:154072
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 8

    Organism-specific databases

    ZFINiZDB-GENE-030616-61. otud5a.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560OTU domain-containing protein 5-APRO_0000278226Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiQ08BW0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08BW0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini211 – 334124OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni216 – 2227Cys-loopBy similarity
    Regioni271 – 28111Variable-loopBy similarityAdd
    BLAST
    Regioni322 – 3276His-loopBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi84 – 17390Gly-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C85 family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG286112.
    GeneTreeiENSGT00510000048473.
    HOGENOMiHOG000231360.
    HOVERGENiHBG060214.
    InParanoidiQ8JFX0.
    KOiK12655.
    OMAiWEDDEIL.
    OrthoDBiEOG77Q4X0.
    PhylomeDBiQ08BW0.
    TreeFamiTF326812.

    Family and domain databases

    InterProiIPR003323. OTU.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q08BW0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTILPKKKPN SSVGVSDHPD DPDRRTGSDP HQHQHQHSHG VRPGARPRAS    50
    PPPWSYQAAP PASREDRRTE SSSRPQQASP PPVGAGSSGG PGDATGMACV 100
    SGNRAELSGG VGCGGGMGGC CSGPGLSKRR RQATCSGGVA GGGTGPGAAG 150
    GGGGGGGGGG VGGPSPEQEE GAGYNSEDEY ENASRLQSED PATVEQQEHW 200
    FEKALQEKKG FVIKKMKEDG ACLFRAVADQ VYGDQDMHEV VRKHCMDYLM 250
    KNADYFSNYV TEDFTTYINR KRKNNCHGNH IEMQAMAEMY NRPVEVYQSG 300
    TEPINTFHGI HQNNDEPIRV SYHRNIHYNS VVNPNKATIG VGLGLPAFKP 350
    GFADQSLMKN AIKTSEESWI EQQMLEDKKR ATDWEATNEA IEEQVARESY 400
    LQWLRDQEKQ ARQPRKASAT CSSATAAASS GLEEWNARSP RQRSSAPSPE 450
    IPDPAHSDTA AKPPSPAGAL ALSKPPSPCA PGPSNQACVG PDRPTSSSLV 500
    SLYPALGYRA IMQEMSPTAF GLTDWEDDEI LASVLAVSQQ EYLDSMKKNA 550
    MHREPSPDSS 560

    Note: No experimental confirmation available.

    Length:560
    Mass (Da):60,038
    Last modified:October 31, 2006 - v1
    Checksum:i425C1E21E8A5A5EA
    GO
    Isoform 2 (identifier: Q08BW0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-236: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:324
    Mass (Da):36,101
    Checksum:iEDD7440593BE68E5
    GO

    Sequence cautioni

    The sequence AAI55305.1 differs from that shown. Reason: BC155304 contains a 74bp deletion, resulting in a frameshift.
    The sequence AAI55305.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 236236Missing in isoform 2. CuratedVSP_034009Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590148 Genomic DNA. Translation: CAD43417.1.
    CR450685 Genomic DNA. Translation: CAQ14391.1.
    CR450685 Genomic DNA. Translation: CAQ14392.1.
    BC124533 mRNA. Translation: AAI24534.1.
    BC155304 mRNA. Translation: AAI55305.1. Sequence problems.
    RefSeqiNP_001068578.1. NM_001075110.1. [Q08BW0-1]
    UniGeneiDr.81817.

    Genome annotation databases

    EnsembliENSDART00000081999; ENSDARP00000076436; ENSDARG00000059006.
    ENSDART00000144986; ENSDARP00000114334; ENSDARG00000059006. [Q08BW0-1]
    GeneIDi555459.
    KEGGidre:555459.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590148 Genomic DNA. Translation: CAD43417.1 .
    CR450685 Genomic DNA. Translation: CAQ14391.1 .
    CR450685 Genomic DNA. Translation: CAQ14392.1 .
    BC124533 mRNA. Translation: AAI24534.1 .
    BC155304 mRNA. Translation: AAI55305.1 . Sequence problems.
    RefSeqi NP_001068578.1. NM_001075110.1. [Q08BW0-1 ]
    UniGenei Dr.81817.

    3D structure databases

    ProteinModelPortali Q08BW0.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C85.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000081999 ; ENSDARP00000076436 ; ENSDARG00000059006 .
    ENSDART00000144986 ; ENSDARP00000114334 ; ENSDARG00000059006 . [Q08BW0-1 ]
    GeneIDi 555459.
    KEGGi dre:555459.

    Organism-specific databases

    CTDi 555459.
    ZFINi ZDB-GENE-030616-61. otud5a.

    Phylogenomic databases

    eggNOGi NOG286112.
    GeneTreei ENSGT00510000048473.
    HOGENOMi HOG000231360.
    HOVERGENi HBG060214.
    InParanoidi Q8JFX0.
    KOi K12655.
    OMAi WEDDEIL.
    OrthoDBi EOG77Q4X0.
    PhylomeDBi Q08BW0.
    TreeFami TF326812.

    Enzyme and pathway databases

    Reactomei REACT_216466. Negative regulators of RIG-I/MDA5 signaling.

    Miscellaneous databases

    NextBioi 20880989.
    PROi Q08BW0.

    Gene expression databases

    Bgeei Q08BW0.

    Family and domain databases

    InterProi IPR003323. OTU.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: WIK.
      Tissue: Embryo and Ovary.

    Entry informationi

    Entry nameiOTU5A_DANRE
    AccessioniPrimary (citable) accession number: Q08BW0
    Secondary accession number(s): A9JTE0
    , B0UYD0, B0UYD1, Q8JFX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3