Q08BW0 (OTU5A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
OTU domain-containing protein 5-A
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme A
Short name=DUBA

Gene names

Name:	otud5a
Synonyms:	otud5
ORF Names:	si:dkey-83k24.3, si:rp71-30i22.1, zgc:154072

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C85 family. Contains 1 OTU domain.
Sequence caution	The sequence AAI55305.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAI55305.1 differs from that shown. Reason: BC155304 contains a 74bp deletion, resulting in a frameshift.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to lipopolysaccharide Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q08BW0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q08BW0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	OTU domain-containing protein 5-A		PRO_0000278226
Regions
	Domain	211 – 334	124	OTU
	Compositional bias	84 – 173	90	Gly-rich
Sites
	Active site	219	1	Potential
	Active site	222	1	Nucleophile By similarity
	Active site	327	1	By similarity
Natural variations
	Alternative sequence	1 – 236	236	Missing in isoform 2.		VSP_034009

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 425C1E21E8A5A5EA	FASTA	560	60,038
	10 20 30 40 50 60 MTILPKKKPN SSVGVSDHPD DPDRRTGSDP HQHQHQHSHG VRPGARPRAS PPPWSYQAAP 70 80 90 100 110 120 PASREDRRTE SSSRPQQASP PPVGAGSSGG PGDATGMACV SGNRAELSGG VGCGGGMGGC 130 140 150 160 170 180 CSGPGLSKRR RQATCSGGVA GGGTGPGAAG GGGGGGGGGG VGGPSPEQEE GAGYNSEDEY 190 200 210 220 230 240 ENASRLQSED PATVEQQEHW FEKALQEKKG FVIKKMKEDG ACLFRAVADQ VYGDQDMHEV 250 260 270 280 290 300 VRKHCMDYLM KNADYFSNYV TEDFTTYINR KRKNNCHGNH IEMQAMAEMY NRPVEVYQSG 310 320 330 340 350 360 TEPINTFHGI HQNNDEPIRV SYHRNIHYNS VVNPNKATIG VGLGLPAFKP GFADQSLMKN 370 380 390 400 410 420 AIKTSEESWI EQQMLEDKKR ATDWEATNEA IEEQVARESY LQWLRDQEKQ ARQPRKASAT 430 440 450 460 470 480 CSSATAAASS GLEEWNARSP RQRSSAPSPE IPDPAHSDTA AKPPSPAGAL ALSKPPSPCA 490 500 510 520 530 540 PGPSNQACVG PDRPTSSSLV SLYPALGYRA IMQEMSPTAF GLTDWEDDEI LASVLAVSQQ 550 560 EYLDSMKKNA MHREPSPDSS
	Isoform 2 [UniParc]. Checksum: EDD7440593BE68E5	FASTA	324	36,101
	10 20 30 40 50 60 MHEVVRKHCM DYLMKNADYF SNYVTEDFTT YINRKRKNNC HGNHIEMQAM AEMYNRPVEV 70 80 90 100 110 120 YQSGTEPINT FHGIHQNNDE PIRVSYHRNI HYNSVVNPNK ATIGVGLGLP AFKPGFADQS 130 140 150 160 170 180 LMKNAIKTSE ESWIEQQMLE DKKRATDWEA TNEAIEEQVA RESYLQWLRD QEKQARQPRK 190 200 210 220 230 240 ASATCSSATA AASSGLEEWN ARSPRQRSSA PSPEIPDPAH SDTAAKPPSP AGALALSKPP 250 260 270 280 290 300 SPCAPGPSNQ ACVGPDRPTS SSLVSLYPAL GYRAIMQEMS PTAFGLTDWE DDEILASVLA 310 320 VSQQEYLDSM KKNAMHREPS PDSS

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: WIK. Tissue: Embryo and Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590148 Genomic DNA. Translation: CAD43417.1. CR450685 Genomic DNA. Translation: CAQ14391.1. CR450685 Genomic DNA. Translation: CAQ14392.1. BC124533 mRNA. Translation: AAI24534.1. BC155304 mRNA. Translation: AAI55305.1. Sequence problems.
IPI	IPI00799602. IPI00896293.
RefSeq	NP_001068578.1. NM_001075110.1.
UniGene	Dr.81817.
3D structure databases
ProteinModelPortal	Q08BW0.
ModBase	Search...
Protein family/group databases
MEROPS	C85.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000081999; ENSDARP00000076436; ENSDARG00000059006. ENSDART00000139150; ENSDARP00000117557; ENSDARG00000059006. ENSDART00000144986; ENSDARP00000114334; ENSDARG00000059006.
GeneID	555459.
KEGG	dre:555459.
Organism-specific databases
CTD	555459.
ZFIN	ZDB-GENE-030616-61. otud5a.
Phylogenomic databases
eggNOG	NOG286112.
GeneTree	ENSGT00510000048473.
HOGENOM	HOG000231360.
HOVERGEN	HBG060214.
InParanoid	Q8JFX0.
KO	K12655.
OMA	WEDDEIL.
OrthoDB	EOG4KWJSV.
Gene expression databases
Bgee	Q08BW0.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20880989.

Entry information

Entry name

OTU5A_DANRE

Accession

Primary (citable) accession number: Q08BW0
Secondary accession number(s): A9JTE0 , B0UYD0, B0UYD1, Q8JFX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	October 31, 2006
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program