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Protein

OTU domain-containing protein 5-A

Gene

otud5a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei219Sequence analysis1
Active sitei222NucleophileBy similarity1
Active sitei327By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC85.001

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5-A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:otud5a
Synonyms:otud5
ORF Names:si:dkey-83k24.3, si:rp71-30i22.1, zgc:154072
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-030616-61 otud5a

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782261 – 560OTU domain-containing protein 5-AAdd BLAST560

Proteomic databases

PaxDbiQ08BW0

Expressioni

Gene expression databases

BgeeiENSDARG00000059006
ExpressionAtlasiQ08BW0 baseline

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000114334

Structurei

3D structure databases

ProteinModelPortaliQ08BW0
SMRiQ08BW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 334OTUPROSITE-ProRule annotationAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 222Cys-loopBy similarity7
Regioni271 – 281Variable-loopBy similarityAdd BLAST11
Regioni322 – 327His-loopBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi84 – 173Gly-richAdd BLAST90

Sequence similaritiesi

Belongs to the peptidase C85 family.Curated

Phylogenomic databases

eggNOGiKOG2605 Eukaryota
ENOG410XNYW LUCA
GeneTreeiENSGT00530000063508
HOGENOMiHOG000231360
HOVERGENiHBG060214
InParanoidiQ08BW0
KOiK12655
OrthoDBiEOG091G0NE6
PhylomeDBiQ08BW0
TreeFamiTF326812

Family and domain databases

InterProiView protein in InterPro
IPR031084 OTU5/OTLD1
IPR003323 OTU_dom
PANTHERiPTHR12419:SF4 PTHR12419:SF4, 1 hit
PfamiView protein in Pfam
PF02338 OTU, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08BW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTILPKKKPN SSVGVSDHPD DPDRRTGSDP HQHQHQHSHG VRPGARPRAS
60 70 80 90 100
PPPWSYQAAP PASREDRRTE SSSRPQQASP PPVGAGSSGG PGDATGMACV
110 120 130 140 150
SGNRAELSGG VGCGGGMGGC CSGPGLSKRR RQATCSGGVA GGGTGPGAAG
160 170 180 190 200
GGGGGGGGGG VGGPSPEQEE GAGYNSEDEY ENASRLQSED PATVEQQEHW
210 220 230 240 250
FEKALQEKKG FVIKKMKEDG ACLFRAVADQ VYGDQDMHEV VRKHCMDYLM
260 270 280 290 300
KNADYFSNYV TEDFTTYINR KRKNNCHGNH IEMQAMAEMY NRPVEVYQSG
310 320 330 340 350
TEPINTFHGI HQNNDEPIRV SYHRNIHYNS VVNPNKATIG VGLGLPAFKP
360 370 380 390 400
GFADQSLMKN AIKTSEESWI EQQMLEDKKR ATDWEATNEA IEEQVARESY
410 420 430 440 450
LQWLRDQEKQ ARQPRKASAT CSSATAAASS GLEEWNARSP RQRSSAPSPE
460 470 480 490 500
IPDPAHSDTA AKPPSPAGAL ALSKPPSPCA PGPSNQACVG PDRPTSSSLV
510 520 530 540 550
SLYPALGYRA IMQEMSPTAF GLTDWEDDEI LASVLAVSQQ EYLDSMKKNA
560
MHREPSPDSS
Note: No experimental confirmation available.
Length:560
Mass (Da):60,038
Last modified:October 31, 2006 - v1
Checksum:i425C1E21E8A5A5EA
GO
Isoform 2 (identifier: Q08BW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.

Note: No experimental confirmation available.
Show »
Length:324
Mass (Da):36,101
Checksum:iEDD7440593BE68E5
GO

Sequence cautioni

The sequence AAI55305 differs from that shown. BC155304 contains a 74bp deletion, resulting in a frameshift.Curated
The sequence AAI55305 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0340091 – 236Missing in isoform 2. CuratedAdd BLAST236

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590148 Genomic DNA Translation: CAD43417.1
CR450685 Genomic DNA Translation: CAQ14391.1
CR450685 Genomic DNA Translation: CAQ14392.1
BC124533 mRNA Translation: AAI24534.1
BC155304 mRNA Translation: AAI55305.1 Sequence problems.
RefSeqiNP_001068578.1, NM_001075110.1 [Q08BW0-1]
UniGeneiDr.81817

Genome annotation databases

EnsembliENSDART00000144986; ENSDARP00000114334; ENSDARG00000059006 [Q08BW0-1]
GeneIDi555459
KEGGidre:555459

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOTU5A_DANRE
AccessioniPrimary (citable) accession number: Q08BW0
Secondary accession number(s): A9JTE0
, B0UYD0, B0UYD1, Q8JFX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health