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Protein

OTU domain-containing protein 5-A

Gene

otud5a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei219 – 2191Sequence Analysis
Active sitei222 – 2221NucleophileBy similarity
Active sitei327 – 3271By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked deubiquitination Source: UniProtKB
  2. protein K63-linked deubiquitination Source: UniProtKB
  3. response to lipopolysaccharide Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_276991. Negative regulators of RIG-I/MDA5 signaling.

Protein family/group databases

MEROPSiC85.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5-A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:otud5a
Synonyms:otud5
ORF Names:si:dkey-83k24.3, si:rp71-30i22.1, zgc:154072
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-030616-61. otud5a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560OTU domain-containing protein 5-APRO_0000278226Add
BLAST

Expressioni

Gene expression databases

BgeeiQ08BW0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 334124OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni216 – 2227Cys-loopBy similarity
Regioni271 – 28111Variable-loopBy similarityAdd
BLAST
Regioni322 – 3276His-loopBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 17390Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C85 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286112.
GeneTreeiENSGT00510000048473.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ08BW0.
KOiK12655.
OMAiHAELGMK.
OrthoDBiEOG77Q4X0.
PhylomeDBiQ08BW0.
TreeFamiTF326812.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08BW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTILPKKKPN SSVGVSDHPD DPDRRTGSDP HQHQHQHSHG VRPGARPRAS
60 70 80 90 100
PPPWSYQAAP PASREDRRTE SSSRPQQASP PPVGAGSSGG PGDATGMACV
110 120 130 140 150
SGNRAELSGG VGCGGGMGGC CSGPGLSKRR RQATCSGGVA GGGTGPGAAG
160 170 180 190 200
GGGGGGGGGG VGGPSPEQEE GAGYNSEDEY ENASRLQSED PATVEQQEHW
210 220 230 240 250
FEKALQEKKG FVIKKMKEDG ACLFRAVADQ VYGDQDMHEV VRKHCMDYLM
260 270 280 290 300
KNADYFSNYV TEDFTTYINR KRKNNCHGNH IEMQAMAEMY NRPVEVYQSG
310 320 330 340 350
TEPINTFHGI HQNNDEPIRV SYHRNIHYNS VVNPNKATIG VGLGLPAFKP
360 370 380 390 400
GFADQSLMKN AIKTSEESWI EQQMLEDKKR ATDWEATNEA IEEQVARESY
410 420 430 440 450
LQWLRDQEKQ ARQPRKASAT CSSATAAASS GLEEWNARSP RQRSSAPSPE
460 470 480 490 500
IPDPAHSDTA AKPPSPAGAL ALSKPPSPCA PGPSNQACVG PDRPTSSSLV
510 520 530 540 550
SLYPALGYRA IMQEMSPTAF GLTDWEDDEI LASVLAVSQQ EYLDSMKKNA
560
MHREPSPDSS

Note: No experimental confirmation available.

Length:560
Mass (Da):60,038
Last modified:October 31, 2006 - v1
Checksum:i425C1E21E8A5A5EA
GO
Isoform 2 (identifier: Q08BW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.

Note: No experimental confirmation available.

Show »
Length:324
Mass (Da):36,101
Checksum:iEDD7440593BE68E5
GO

Sequence cautioni

The sequence AAI55305.1 differs from that shown.BC155304 contains a 74bp deletion, resulting in a frameshift.Curated
The sequence AAI55305.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 236236Missing in isoform 2. CuratedVSP_034009Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590148 Genomic DNA. Translation: CAD43417.1.
CR450685 Genomic DNA. Translation: CAQ14391.1.
CR450685 Genomic DNA. Translation: CAQ14392.1.
BC124533 mRNA. Translation: AAI24534.1.
BC155304 mRNA. Translation: AAI55305.1. Sequence problems.
RefSeqiNP_001068578.1. NM_001075110.1. [Q08BW0-1]
UniGeneiDr.81817.

Genome annotation databases

EnsembliENSDART00000144986; ENSDARP00000114334; ENSDARG00000059006. [Q08BW0-1]
GeneIDi555459.
KEGGidre:555459.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590148 Genomic DNA. Translation: CAD43417.1.
CR450685 Genomic DNA. Translation: CAQ14391.1.
CR450685 Genomic DNA. Translation: CAQ14392.1.
BC124533 mRNA. Translation: AAI24534.1.
BC155304 mRNA. Translation: AAI55305.1. Sequence problems.
RefSeqiNP_001068578.1. NM_001075110.1. [Q08BW0-1]
UniGeneiDr.81817.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC85.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000144986; ENSDARP00000114334; ENSDARG00000059006. [Q08BW0-1]
GeneIDi555459.
KEGGidre:555459.

Organism-specific databases

CTDi555459.
ZFINiZDB-GENE-030616-61. otud5a.

Phylogenomic databases

eggNOGiNOG286112.
GeneTreeiENSGT00510000048473.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ08BW0.
KOiK12655.
OMAiHAELGMK.
OrthoDBiEOG77Q4X0.
PhylomeDBiQ08BW0.
TreeFamiTF326812.

Enzyme and pathway databases

ReactomeiREACT_276991. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

NextBioi20880989.
PROiQ08BW0.

Gene expression databases

BgeeiQ08BW0.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: WIK.
    Tissue: Embryo and Ovary.

Entry informationi

Entry nameiOTU5A_DANRE
AccessioniPrimary (citable) accession number: Q08BW0
Secondary accession number(s): A9JTE0
, B0UYD0, B0UYD1, Q8JFX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 31, 2006
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.