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Q08BW0

- OTU5A_DANRE

UniProt

Q08BW0 - OTU5A_DANRE

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Protein
OTU domain-containing protein 5-A
Gene
otud5a, otud5, si:dkey-83k24.3, si:rp71-30i22.1, zgc:154072
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei219 – 2191 Reviewed prediction
Active sitei222 – 2221Nucleophile By similarity
Active sitei327 – 3271 By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked deubiquitination Source: UniProtKB
  2. protein K63-linked deubiquitination Source: UniProtKB
  3. response to lipopolysaccharide Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_216466. Negative regulators of RIG-I/MDA5 signaling.

Protein family/group databases

MEROPSiC85.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5-A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:otud5a
Synonyms:otud5
ORF Names:si:dkey-83k24.3, si:rp71-30i22.1, zgc:154072
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-030616-61. otud5a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560OTU domain-containing protein 5-A
PRO_0000278226Add
BLAST

Expressioni

Gene expression databases

BgeeiQ08BW0.

Structurei

3D structure databases

ProteinModelPortaliQ08BW0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 334124OTU
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni216 – 2227Cys-loop By similarity
Regioni271 – 28111Variable-loop By similarity
Add
BLAST
Regioni322 – 3276His-loop By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 17390Gly-rich
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C85 family.
Contains 1 OTU domain.

Phylogenomic databases

eggNOGiNOG286112.
GeneTreeiENSGT00510000048473.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ8JFX0.
KOiK12655.
OMAiWEDDEIL.
OrthoDBiEOG77Q4X0.
PhylomeDBiQ08BW0.
TreeFamiTF326812.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q08BW0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTILPKKKPN SSVGVSDHPD DPDRRTGSDP HQHQHQHSHG VRPGARPRAS    50
PPPWSYQAAP PASREDRRTE SSSRPQQASP PPVGAGSSGG PGDATGMACV 100
SGNRAELSGG VGCGGGMGGC CSGPGLSKRR RQATCSGGVA GGGTGPGAAG 150
GGGGGGGGGG VGGPSPEQEE GAGYNSEDEY ENASRLQSED PATVEQQEHW 200
FEKALQEKKG FVIKKMKEDG ACLFRAVADQ VYGDQDMHEV VRKHCMDYLM 250
KNADYFSNYV TEDFTTYINR KRKNNCHGNH IEMQAMAEMY NRPVEVYQSG 300
TEPINTFHGI HQNNDEPIRV SYHRNIHYNS VVNPNKATIG VGLGLPAFKP 350
GFADQSLMKN AIKTSEESWI EQQMLEDKKR ATDWEATNEA IEEQVARESY 400
LQWLRDQEKQ ARQPRKASAT CSSATAAASS GLEEWNARSP RQRSSAPSPE 450
IPDPAHSDTA AKPPSPAGAL ALSKPPSPCA PGPSNQACVG PDRPTSSSLV 500
SLYPALGYRA IMQEMSPTAF GLTDWEDDEI LASVLAVSQQ EYLDSMKKNA 550
MHREPSPDSS 560

Note: No experimental confirmation available.

Length:560
Mass (Da):60,038
Last modified:October 31, 2006 - v1
Checksum:i425C1E21E8A5A5EA
GO
Isoform 2 (identifier: Q08BW0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.

Note: No experimental confirmation available.

Show »
Length:324
Mass (Da):36,101
Checksum:iEDD7440593BE68E5
GO

Sequence cautioni

The sequence AAI55305.1 differs from that shown. Reason: BC155304 contains a 74bp deletion, resulting in a frameshift.
The sequence AAI55305.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 236236Missing in isoform 2.
VSP_034009Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590148 Genomic DNA. Translation: CAD43417.1.
CR450685 Genomic DNA. Translation: CAQ14391.1.
CR450685 Genomic DNA. Translation: CAQ14392.1.
BC124533 mRNA. Translation: AAI24534.1.
BC155304 mRNA. Translation: AAI55305.1. Sequence problems.
RefSeqiNP_001068578.1. NM_001075110.1. [Q08BW0-1]
UniGeneiDr.81817.

Genome annotation databases

EnsembliENSDART00000081999; ENSDARP00000076436; ENSDARG00000059006.
ENSDART00000144986; ENSDARP00000114334; ENSDARG00000059006. [Q08BW0-1]
GeneIDi555459.
KEGGidre:555459.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590148 Genomic DNA. Translation: CAD43417.1 .
CR450685 Genomic DNA. Translation: CAQ14391.1 .
CR450685 Genomic DNA. Translation: CAQ14392.1 .
BC124533 mRNA. Translation: AAI24534.1 .
BC155304 mRNA. Translation: AAI55305.1 . Sequence problems.
RefSeqi NP_001068578.1. NM_001075110.1. [Q08BW0-1 ]
UniGenei Dr.81817.

3D structure databases

ProteinModelPortali Q08BW0.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C85.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000081999 ; ENSDARP00000076436 ; ENSDARG00000059006 .
ENSDART00000144986 ; ENSDARP00000114334 ; ENSDARG00000059006 . [Q08BW0-1 ]
GeneIDi 555459.
KEGGi dre:555459.

Organism-specific databases

CTDi 555459.
ZFINi ZDB-GENE-030616-61. otud5a.

Phylogenomic databases

eggNOGi NOG286112.
GeneTreei ENSGT00510000048473.
HOGENOMi HOG000231360.
HOVERGENi HBG060214.
InParanoidi Q8JFX0.
KOi K12655.
OMAi WEDDEIL.
OrthoDBi EOG77Q4X0.
PhylomeDBi Q08BW0.
TreeFami TF326812.

Enzyme and pathway databases

Reactomei REACT_216466. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

NextBioi 20880989.
PROi Q08BW0.

Gene expression databases

Bgeei Q08BW0.

Family and domain databases

InterProi IPR003323. OTU.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: WIK.
    Tissue: Embryo and Ovary.

Entry informationi

Entry nameiOTU5A_DANRE
AccessioniPrimary (citable) accession number: Q08BW0
Secondary accession number(s): A9JTE0
, B0UYD0, B0UYD1, Q8JFX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 31, 2006
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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