Q08BH7 (UBE2T_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 T
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein T
Ubiquitin-protein ligase T

Gene names

Name:	ube2t
ORF Names:	zgc:153687

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in DNA repair By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Nucleus By similarity. Note: Accumulates to chromatin By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from sequence or structural similarity. Source: UniProtKB protein autoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein monoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Ubiquitin-conjugating enzyme E2 T		PRO_0000416880
Sites
	Active site	86	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08BH7 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 540F3B0296275816	FASTA	194	21,891
	10 20 30 40 50 60 MQRVSRLKRE MQLLTAEPPP GVSCWQSEGR LDELQAQIVG GANTPYEGGV FTLEINIPER 70 80 90 100 110 120 YPFEPPKMRF LTPIYHPNID NAGRICLDAL KLPPKGAWRP SLNISTVLTS IQLLMAEPNP 130 140 150 160 170 180 DDPLMADISS EFKYNKPLYL EKAKKWTAEH AIQKNKGCVE TDGKTPENKN LKTSHKREAL 190 SAQENLEHTK KVCL

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CABZ01030035 Genomic DNA. No translation available. BC124718 mRNA. Translation: AAI24719.1.
IPI	IPI00803367.
RefSeq	NP_001070763.1. NM_001077295.2.
UniGene	Dr.81555.
3D structure databases
ProteinModelPortal	Q08BH7.
SMR	Q08BH7. Positions 1-154.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000086755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	768152.
KEGG	dre:768152.
Organism-specific databases
CTD	29089.
ZFIN	ZDB-GENE-061013-547. ube2t.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
InParanoid	Q08BH7.
KO	K13960.
OMA	GITCWQE.
OrthoDB	EOG40GCRT.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q08BH7.
Bgee	Q08BH7.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20918470.

Entry information

Entry name

UBE2T_DANRE

Accession

Primary (citable) accession number: Q08BH7
Secondary accession number(s): F1R970

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	October 31, 2006
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program