Reviewed, UniProtKB/Swiss-Prot Q08B39 (HOT_XENLA)

Names and origin

Protein names

Recommended name:
    Hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24
Alternative name(s):
    Alcohol dehydrogenase iron-containing protein 1

Gene names

Name:

adhfe1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 463		Hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000323000

Sequences

	Sequence		Length	Mass (Da)
	Q08B39-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 5E373B5086BA88AE	FASTA	463	50,172
	10 20 30 40 50 60 MAAGRDGVIH LLRQLQRASC RCPAHSHTYS QAPATARTTD YAFEMAVSSI RYGENVTQEI 70 80 90 100 110 120 GMDLQNWGTR NVCVMTDRNL SELSPVKAVL NSLVKNGINF KLYDSVRVEP TDKSFMDAIE 130 140 150 160 170 180 FAKKGQFDAF VAVGGGSVID TCKAANLYSS SPDSDFLDYV NPPIGKGKAV TVPLKPLIAV 190 200 210 220 230 240 PTTSGTGSET TGIAIFDYEE LKAKTGIASR AIKPTLGLID PAHTLSMPER VVANSGFDVL 250 260 270 280 290 300 CHSLESYTAL PYNMRSPCPT NPINRPAYQG SNPISDVWAK HALRIVAKFL KRAVRNPDDR 310 320 330 340 350 360 EARFAMHLAS SFAGVGFGNA GVHLCHGMSY PIAGHVKTYR AKDYKVDHPL VPHGLSVVLT 370 380 390 400 410 420 SPAVFSFTGL MCPERHLEAA EILGADLRTA KIKDAGLILA DTLRKFLYDL NVDDGLAAVG 430 440 450 460 YTAEDIPALV KGTLPQERVT KLSPRAHSEE ELAALFEASM KLY

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC124888 mRNA. Translation: AAI24889.1.
RefSeq	NP_001121274.1.
UniGene	Xl.15864
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100158357.
KEGG	xla:100158357.
Organism-specific databases
Xenbase	XB-FEAT-966428. adhfe1.
Phylogenomic databases
HOVERGEN	Q08B39.
Enzyme and pathway databases
BRENDA	1.1.99.24. 648.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_XENLA

Accession

Primary (citable) accession number: Q08B39

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 31, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project