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Q08B39 (HOT_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyacid-oxoacid transhydrogenase, mitochondrial

Short name=HOT
EC=1.1.99.24
Alternative name(s):
Alcohol dehydrogenase iron-containing protein 1
Short name=ADHFe1
Gene names
Name:adhfe1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraMesobatrachiaPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.

Catalytic activity

(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.

4-hydroxybutanoate + 2-oxoglutarate = succinic semialdehyde + (R)-2-hydroxyglutarate.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processmolecular hydrogen transport

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentmitochondrion

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular functionhydroxyacid-oxoacid transhydrogenase activity

Inferred from sequence or structural similarity. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 463Hydroxyacid-oxoacid transhydrogenase, mitochondrialPRO_0000323000

Sequences

Sequence LengthMass (Da)Tools
Q08B39 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: 5E373B5086BA88AE

FASTA46350,172
        10         20         30         40         50         60 
MAAGRDGVIH LLRQLQRASC RCPAHSHTYS QAPATARTTD YAFEMAVSSI RYGENVTQEI 

        70         80         90        100        110        120 
GMDLQNWGTR NVCVMTDRNL SELSPVKAVL NSLVKNGINF KLYDSVRVEP TDKSFMDAIE 

       130        140        150        160        170        180 
FAKKGQFDAF VAVGGGSVID TCKAANLYSS SPDSDFLDYV NPPIGKGKAV TVPLKPLIAV 

       190        200        210        220        230        240 
PTTSGTGSET TGIAIFDYEE LKAKTGIASR AIKPTLGLID PAHTLSMPER VVANSGFDVL 

       250        260        270        280        290        300 
CHSLESYTAL PYNMRSPCPT NPINRPAYQG SNPISDVWAK HALRIVAKFL KRAVRNPDDR 

       310        320        330        340        350        360 
EARFAMHLAS SFAGVGFGNA GVHLCHGMSY PIAGHVKTYR AKDYKVDHPL VPHGLSVVLT 

       370        380        390        400        410        420 
SPAVFSFTGL MCPERHLEAA EILGADLRTA KIKDAGLILA DTLRKFLYDL NVDDGLAAVG 

       430        440        450        460 
YTAEDIPALV KGTLPQERVT KLSPRAHSEE ELAALFEASM KLY 

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References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC124888 mRNA. Translation: AAI24889.1.
RefSeqNP_001121274.1. NM_001127802.1.
UniGeneXl.15864.

3D structure databases

ProteinModelPortalQ08B39.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100158357.
KEGGxla:100158357.

Organism-specific databases

CTD137872.
XenbaseXB-GENE-966434. adhfe1.

Phylogenomic databases

HOVERGENHBG057032.

Family and domain databases

InterProIPR001670. ADH_Fe.
[Graphical view]
KOK11173.
PfamPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEPS00913. ADH_IRON_1. False negative.
PS00060. ADH_IRON_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOT_XENLA
AccessionPrimary (citable) accession number: Q08B39
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families