Reviewed, UniProtKB/Swiss-Prot Q08AE8 (SPIR1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein spire homolog 1
      Short name=Spir-1

Gene names

Name:	SPIRE1
Synonyms:	KIAA1135, SPIR1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport. Ref.5 UniProtKB Q9U1K1
Subcellular location	Cytoplasm › cytoskeleton. Cytoplasm › perinuclear region. Note: Punctate spots in perinuclear region and cytoplasm, co-localised with Rab11. Ref.5
Domain	Binds to actin monomers via the WH2 domain By similarity. UniProtKB Q9U1K1 The Spir-box targets binding to intracellular membrane structures. Ref.5
Sequence similarities	Belongs to the spire family. Contains 1 KIND domain. Contains 2 WH2 domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAQ	P27348	1	EBI-1055655,EBI-359854

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.4 (identifier: Q08AE8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 Ref.5 Ref.4 (identifier: Q08AE8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-410: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	Protein spire homolog 1		PRO_0000309569
Regions
	Domain	40 – 231	192	KIND
	Domain	305 – 323	19	WH2 1
	Domain	369 – 386	18	WH2 2
	Region	556 – 576	21	Spir-box
	Coiled coil	229 – 257	29	Potential
Amino acid modifications
	Modified residue	464	1	Phosphoserine Ref.6 Ref.7
	Modified residue	465	1	Phosphoserine Ref.6 Ref.7
	Modified residue	467	1	Phosphoserine Ref.6 Ref.7
Natural variations
	Alternative sequence	397 – 410	14	Missing in isoform 2.		VSP_052595
Experimental info
	Sequence conflict	410	1	P → S in BAA86449. Ref.1
	Sequence conflict	410	1	P → S in AAI25207. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 0B83C3B1BC852040	FASTA	756	85,554
	10 20 30 40 50 60 MAQAAGPAGG GEPRTEAVGG EGPREPGAAG GAAGGSRDAL SLEEILRLYN QPINEEQAWA 70 80 90 100 110 120 VCYQCCGSLR AAARRRQPRH RVRSAAQIRV WRDGAVTLAP AADDAGEPPP VAGKLGYSQC 130 140 150 160 170 180 METEVIESLG IIIYKALDYG LKENEERELS PPLEQLIDHM ANTVEADGSN DEGYEAAEEG 190 200 210 220 230 240 LGDEDEKRKI SAIRSYRDVM KLCAAHLPTE SDAPNHYQAV CRALFAETME LHTFLTKIKS 250 260 270 280 290 300 AKENLKKIQE MEKSDESSTD LEELKNADWA RFWVQVMRDL RNGVKLKKVQ ERQYNPLPIE 310 320 330 340 350 360 YQLTPYEMLM DDIRCKRYTL RKVMVNGDIP PRLKKSAHEI ILDFIRSRPP LNPVSARKLK 370 380 390 400 410 420 PTPPRPRSLH ERILEEIKAE RKLRPVSPEE IRRSRLAMRP LSMSYSFDLP DVTTPESTKN 430 440 450 460 470 480 LVESSMVNGG LTSQTKENGL STSQQVPAQR KKLLRAPTLA ELDSSESEEE TLHKSTSSSS 490 500 510 520 530 540 VSPSFPEEPV LEAVSTRKKP PKFLPISSTP QPERRQPPQR RHSIEKETPT NVRQFLPPSR 550 560 570 580 590 600 QSSRSLEEFC YPVECLALTV EEVMHIRQVL VKAELEKYQQ YKDIYTALKK GKLCFCCRTR 610 620 630 640 650 660 RFSFFTWSYT CQFCKRPVCS QCCKKMRLPS KPYSTLPIFS LGPSALQRGE SSMRSEKPST 670 680 690 700 710 720 AHHRPLRSIA RFSSKSKSMD KSDEELQFPK ELMEDWSTME VCVDCKKFIS EIISSSRRSL 730 740 750 VLANKRARLK RKTQSFYMSS PGPSEYCPSE RTISEI
	Isoform 2. Checksum: F76F168197958220	FASTA	742	83,957
	10 20 30 40 50 60 MAQAAGPAGG GEPRTEAVGG EGPREPGAAG GAAGGSRDAL SLEEILRLYN QPINEEQAWA 70 80 90 100 110 120 VCYQCCGSLR AAARRRQPRH RVRSAAQIRV WRDGAVTLAP AADDAGEPPP VAGKLGYSQC 130 140 150 160 170 180 METEVIESLG IIIYKALDYG LKENEERELS PPLEQLIDHM ANTVEADGSN DEGYEAAEEG 190 200 210 220 230 240 LGDEDEKRKI SAIRSYRDVM KLCAAHLPTE SDAPNHYQAV CRALFAETME LHTFLTKIKS 250 260 270 280 290 300 AKENLKKIQE MEKSDESSTD LEELKNADWA RFWVQVMRDL RNGVKLKKVQ ERQYNPLPIE 310 320 330 340 350 360 YQLTPYEMLM DDIRCKRYTL RKVMVNGDIP PRLKKSAHEI ILDFIRSRPP LNPVSARKLK 370 380 390 400 410 420 PTPPRPRSLH ERILEEIKAE RKLRPVSPEE IRRSRLDVTT PESTKNLVES SMVNGGLTSQ 430 440 450 460 470 480 TKENGLSTSQ QVPAQRKKLL RAPTLAELDS SESEEETLHK STSSSSVSPS FPEEPVLEAV 490 500 510 520 530 540 STRKKPPKFL PISSTPQPER RQPPQRRHSI EKETPTNVRQ FLPPSRQSSR SLEEFCYPVE 550 560 570 580 590 600 CLALTVEEVM HIRQVLVKAE LEKYQQYKDI YTALKKGKLC FCCRTRRFSF FTWSYTCQFC 610 620 630 640 650 660 KRPVCSQCCK KMRLPSKPYS TLPIFSLGPS ALQRGESSMR SEKPSTAHHR PLRSIARFSS 670 680 690 700 710 720 KSKSMDKSDE ELQFPKELME DWSTMEVCVD CKKFISEIIS SSRRSLVLAN KRARLKRKTQ 730 740 SFYMSSPGPS EYCPSERTIS EI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain." Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O. DNA Res. 6:329-336(1999) [PubMed: 10574461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 44-756 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-756 (ISOFORM 1). Tissue: Embryonic stem cell.
[5]	"The Spir actin organizers are involved in vesicle transport processes." Kerkhoff E., Simpson J.C., Leberfinger C.B., Otto I.M., Doerks T., Bork P., Rapp U.R., Raabe T., Pepperkok R. Curr. Biol. 11:1963-1968(2001) [PubMed: 11747823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 123-756 (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION. Tissue: Brain and Testis.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464; SER-465 AND SER-467, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464; SER-465 AND SER-467, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB032961 mRNA. Translation: BAA86449.2. Different initiation. AP005482 Genomic DNA. No translation available. AP001029 Genomic DNA. No translation available. BC115005 mRNA. Translation: AAI15006.1. Different initiation. BC125206 mRNA. Translation: AAI25207.1. Different initiation. BC125207 mRNA. Translation: AAI25208.1. Different initiation. AJ277587 mRNA. Translation: CAB96370.1. Different initiation.
IPI	IPI00171145. IPI00645268.
RefSeq	NP_001122098.1. NP_001122099.1. NP_064533.3.
UniGene	Hs.515283
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08AE8. 1 interaction.
PTM databases
PhosphoSite	Q08AE8.
Proteomic databases
PRIDE	Q08AE8.
Genome annotation databases
Ensembl	ENSG00000134278. Homo sapiens. [Contig view]
GeneID	56907.
KEGG	hsa:56907.
UCSC	uc002krd.1. human. uc002kre.1. human.
Organism-specific databases
GeneCards	GC18M012438.
HGNC	HGNC:30622. SPIRE1.
MIM	609216. gene.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q08AE8.
Gene expression databases
Bgee	Q08AE8.
CleanEx	HS_SPIRE1.
Family and domain databases
InterPro	IPR011019. KIND. [Graphical view]
PROSITE	PS51377. KIND. 1 hit. PS51082. WH2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	62379.
SOURCE	Search...

Entry information

Entry name

SPIR1_HUMAN

Accession

Primary (citable) accession number: Q08AE8
Secondary accession number(s): Q1RMD4, Q9NQ71, Q9ULT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 25, 2008
Last modified:	July 7, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)