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Protein

Protein spire homolog 1

Gene

SPIRE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spire homolog 1
Short name:
Spir-1
Gene namesi
Name:SPIRE1Imported
Synonyms:KIAA1135, SPIR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:30622. SPIRE1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131I → K: Strongly reduces interaction with FMN2. 1 Publication1
Mutagenesisi134Y → K: Abolishes interaction with FMN2. 1 Publication1
Mutagenesisi138D → N: Abolishes interaction with FMN2. 2 Publications1
Mutagenesisi146E → A or K: Abolishes interaction with FMN2. 1 Publication1

Organism-specific databases

DisGeNETi56907.
OpenTargetsiENSG00000134278.
PharmGKBiPA134895885.

Polymorphism and mutation databases

BioMutaiSPIRE1.
DMDMi425906061.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003095692 – 756Protein spire homolog 1Add BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei406PhosphoserineBy similarity1
Modified residuei464PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei509PhosphothreonineCombined sources1
Modified residuei678PhosphoserineCombined sources1
Modified residuei682PhosphoserineBy similarity1
Modified residuei735PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08AE8.
PaxDbiQ08AE8.
PeptideAtlasiQ08AE8.
PRIDEiQ08AE8.

PTM databases

iPTMnetiQ08AE8.
PhosphoSitePlusiQ08AE8.

Expressioni

Gene expression databases

BgeeiENSG00000134278.
CleanExiHS_SPIRE1.
ExpressionAtlasiQ08AE8. baseline and differential.
GenevisibleiQ08AE8. HS.

Organism-specific databases

HPAiHPA040737.
HPA040942.

Interactioni

Subunit structurei

Interacts with FMN2.2 Publications

Protein-protein interaction databases

BioGridi121237. 6 interactors.
DIPiDIP-42378N.
IntActiQ08AE8. 3 interactors.
MINTiMINT-3975323.
STRINGi9606.ENSP00000387266.

Structurei

Secondary structure

1756
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 41Combined sources4
Helixi42 – 49Combined sources8
Helixi55 – 74Combined sources20
Helixi85 – 87Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi96 – 98Combined sources3
Beta strandi116 – 120Combined sources5
Helixi122 – 137Combined sources16
Turni138 – 140Combined sources3
Beta strandi145 – 147Combined sources3
Helixi151 – 160Combined sources10
Helixi196 – 204Combined sources9
Beta strandi207 – 209Combined sources3
Helixi210 – 212Combined sources3
Helixi213 – 234Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YLEX-ray1.80A36-236[»]
2YLFX-ray2.05A36-236[»]
3R7GX-ray2.20A20-237[»]
3RBWX-ray3.20A/B/C/D20-237[»]
ProteinModelPortaliQ08AE8.
SMRiQ08AE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 231KINDPROSITE-ProRule annotationAdd BLAST192
Domaini305 – 323WH2 1Add BLAST19
Domaini369 – 386WH2 2Add BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 138Important for interaction with FMN28
Regioni556 – 576Spir-boxAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili229 – 257Sequence analysisAdd BLAST29

Domaini

Binds to actin monomers via the WH2 domain.By similarity
The Spir-box targets binding to intracellular membrane structures.1 Publication

Sequence similaritiesi

Belongs to the spire family.Curated
Contains 1 KIND domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.Sequence analysis

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ08AE8.
KOiK02098.
OMAiTMELYTF.
OrthoDBiEOG091G0EAQ.
PhylomeDBiQ08AE8.
TreeFamiTF326239.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029905. Spire1.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 1 hit.
PTHR21345:SF2. PTHR21345:SF2. 1 hit.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q08AE8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQAAGPAGG GEPRTEAVGG EGPREPGAAG GAAGGSRDAL SLEEILRLYN
60 70 80 90 100
QPINEEQAWA VCYQCCGSLR AAARRRQPRH RVRSAAQIRV WRDGAVTLAP
110 120 130 140 150
AADDAGEPPP VAGKLGYSQC METEVIESLG IIIYKALDYG LKENEERELS
160 170 180 190 200
PPLEQLIDHM ANTVEADGSN DEGYEAAEEG LGDEDEKRKI SAIRSYRDVM
210 220 230 240 250
KLCAAHLPTE SDAPNHYQAV CRALFAETME LHTFLTKIKS AKENLKKIQE
260 270 280 290 300
MEKSDESSTD LEELKNADWA RFWVQVMRDL RNGVKLKKVQ ERQYNPLPIE
310 320 330 340 350
YQLTPYEMLM DDIRCKRYTL RKVMVNGDIP PRLKKSAHEI ILDFIRSRPP
360 370 380 390 400
LNPVSARKLK PTPPRPRSLH ERILEEIKAE RKLRPVSPEE IRRSRLAMRP
410 420 430 440 450
LSMSYSFDLS DVTTPESTKN LVESSMVNGG LTSQTKENGL STSQQVPAQR
460 470 480 490 500
KKLLRAPTLA ELDSSESEEE TLHKSTSSSS VSPSFPEEPV LEAVSTRKKP
510 520 530 540 550
PKFLPISSTP QPERRQPPQR RHSIEKETPT NVRQFLPPSR QSSRSLEEFC
560 570 580 590 600
YPVECLALTV EEVMHIRQVL VKAELEKYQQ YKDIYTALKK GKLCFCCRTR
610 620 630 640 650
RFSFFTWSYT CQFCKRPVCS QCCKKMRLPS KPYSTLPIFS LGPSALQRGE
660 670 680 690 700
SSMRSEKPST AHHRPLRSIA RFSSKSKSMD KSDEELQFPK ELMEDWSTME
710 720 730 740 750
VCVDCKKFIS EIISSSRRSL VLANKRARLK RKTQSFYMSS PGPSEYCPSE

RTISEI
Note: No experimental confirmation available.Curated
Length:756
Mass (Da):85,544
Last modified:November 28, 2012 - v3
Checksum:i1379B39C8CA9DDB3
GO
Isoform 5 (identifier: Q08AE8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.
     397-410: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:622
Mass (Da):71,568
Checksum:iD4DF3C072A295F96
GO
Isoform 22 Publications (identifier: Q08AE8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-410: Missing.

Show »
Length:742
Mass (Da):83,957
Checksum:iF76F168197958220
GO
Isoform 3 (identifier: Q08AE8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     397-410: Missing.

Show »
Length:583
Mass (Da):67,057
Checksum:i89163C062A16AB3D
GO
Isoform 4 (identifier: Q08AE8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     397-410: Missing.
     616-756: RPVCSQCCKK...CPSERTISEI → SDCLFNGTAHCQFYLG

Note: No experimental confirmation available.
Show »
Length:458
Mass (Da):52,747
Checksum:iBCE16535DEA4326C
GO

Sequence cautioni

The sequence AAI15006 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI25207 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI25208 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA86449 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058695249Q → P.Corresponds to variant rs1785296dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0379251 – 159Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST159
Alternative sequenceiVSP_0544641 – 120Missing in isoform 5. CuratedAdd BLAST120
Alternative sequenceiVSP_052595397 – 410Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST14
Alternative sequenceiVSP_037926616 – 756RPVCS…TISEI → SDCLFNGTAHCQFYLG in isoform 4. 1 PublicationAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032961 mRNA. Translation: BAA86449.2. Different initiation.
AK290180 mRNA. Translation: BAF82869.1.
AL833817 mRNA. Translation: CAD38680.1.
AP001028 Genomic DNA. No translation available.
AP001029 Genomic DNA. No translation available.
AP005482 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01546.1.
BC115005 mRNA. Translation: AAI15006.1. Different initiation.
BC125206 mRNA. Translation: AAI25207.1. Different initiation.
BC125207 mRNA. Translation: AAI25208.1. Different initiation.
AJ277587 mRNA. Translation: CAB96370.1.
CCDSiCCDS32790.2. [Q08AE8-2]
CCDS45829.1. [Q08AE8-1]
CCDS45830.1. [Q08AE8-5]
RefSeqiNP_001122098.1. NM_001128626.1. [Q08AE8-1]
NP_001122099.1. NM_001128627.1. [Q08AE8-5]
NP_064533.3. NM_020148.2. [Q08AE8-2]
UniGeneiHs.515283.

Genome annotation databases

EnsembliENST00000409402; ENSP00000387266; ENSG00000134278. [Q08AE8-1]
ENST00000410092; ENSP00000387226; ENSG00000134278. [Q08AE8-2]
ENST00000440472; ENSP00000404752; ENSG00000134278. [Q08AE8-4]
ENST00000453447; ENSP00000407050; ENSG00000134278. [Q08AE8-5]
GeneIDi56907.
KEGGihsa:56907.
UCSCiuc002kre.4. human. [Q08AE8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032961 mRNA. Translation: BAA86449.2. Different initiation.
AK290180 mRNA. Translation: BAF82869.1.
AL833817 mRNA. Translation: CAD38680.1.
AP001028 Genomic DNA. No translation available.
AP001029 Genomic DNA. No translation available.
AP005482 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01546.1.
BC115005 mRNA. Translation: AAI15006.1. Different initiation.
BC125206 mRNA. Translation: AAI25207.1. Different initiation.
BC125207 mRNA. Translation: AAI25208.1. Different initiation.
AJ277587 mRNA. Translation: CAB96370.1.
CCDSiCCDS32790.2. [Q08AE8-2]
CCDS45829.1. [Q08AE8-1]
CCDS45830.1. [Q08AE8-5]
RefSeqiNP_001122098.1. NM_001128626.1. [Q08AE8-1]
NP_001122099.1. NM_001128627.1. [Q08AE8-5]
NP_064533.3. NM_020148.2. [Q08AE8-2]
UniGeneiHs.515283.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YLEX-ray1.80A36-236[»]
2YLFX-ray2.05A36-236[»]
3R7GX-ray2.20A20-237[»]
3RBWX-ray3.20A/B/C/D20-237[»]
ProteinModelPortaliQ08AE8.
SMRiQ08AE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121237. 6 interactors.
DIPiDIP-42378N.
IntActiQ08AE8. 3 interactors.
MINTiMINT-3975323.
STRINGi9606.ENSP00000387266.

PTM databases

iPTMnetiQ08AE8.
PhosphoSitePlusiQ08AE8.

Polymorphism and mutation databases

BioMutaiSPIRE1.
DMDMi425906061.

Proteomic databases

MaxQBiQ08AE8.
PaxDbiQ08AE8.
PeptideAtlasiQ08AE8.
PRIDEiQ08AE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409402; ENSP00000387266; ENSG00000134278. [Q08AE8-1]
ENST00000410092; ENSP00000387226; ENSG00000134278. [Q08AE8-2]
ENST00000440472; ENSP00000404752; ENSG00000134278. [Q08AE8-4]
ENST00000453447; ENSP00000407050; ENSG00000134278. [Q08AE8-5]
GeneIDi56907.
KEGGihsa:56907.
UCSCiuc002kre.4. human. [Q08AE8-1]

Organism-specific databases

CTDi56907.
DisGeNETi56907.
GeneCardsiSPIRE1.
HGNCiHGNC:30622. SPIRE1.
HPAiHPA040737.
HPA040942.
MIMi609216. gene.
neXtProtiNX_Q08AE8.
OpenTargetsiENSG00000134278.
PharmGKBiPA134895885.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ08AE8.
KOiK02098.
OMAiTMELYTF.
OrthoDBiEOG091G0EAQ.
PhylomeDBiQ08AE8.
TreeFamiTF326239.

Miscellaneous databases

ChiTaRSiSPIRE1. human.
GenomeRNAii56907.
PROiQ08AE8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134278.
CleanExiHS_SPIRE1.
ExpressionAtlasiQ08AE8. baseline and differential.
GenevisibleiQ08AE8. HS.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029905. Spire1.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 1 hit.
PTHR21345:SF2. PTHR21345:SF2. 1 hit.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPIR1_HUMAN
AccessioniPrimary (citable) accession number: Q08AE8
Secondary accession number(s): A8K2B5
, J3KQ50, J3KQR5, Q1RMD4, Q8NDP1, Q9NQ71, Q9ULT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 28, 2012
Last modified: November 30, 2016
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.