Q089E1 (PLSB_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Sfri_0261

Organism

Shewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]

Taxonomic identifier

318167 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049457
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q089E1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: F3AAA95E1FBB6CED	FASTA	807	91,990
	10 20 30 40 50 60 MSKPDSIFLR ALRWIQKWMV QTIVVPHDPF DDLNIDPTKP LVYLMKTESI SDIAALSEIT 70 80 90 100 110 120 EGFGLPSPYE PLQLDGLTVP RVVCLEGRKP LFGKRESGDK FLNYFTSLLS LHSESPELDI 130 140 150 160 170 180 QLVPVCLYWG RTPGKEEDSM KAAVFERENP TWLRKWLMIL FLGRHNFVQF SNALSLRHMA 190 200 210 220 230 240 DEHGTDKRIA HKLTRVARVH FRRQRKVMTG PQLPNRQALF ASLLKSESIK KAIEEESANK 250 260 270 280 290 300 KVTVEKARET AIEYLDEIAA DYSDSLVRIA ERFLTWLWNK LYSGINIKGA EQVRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLLSY ILYYQGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFN 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFAKG YAVEYFTEGG RSRTGRLLAP KTGMLAMTIN SVLRGIERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKKKE SVWQVFGAIR KLGNFGQGYV NFGEPINLQQ 490 500 510 520 530 540 FLNQQAPEWR DELAKDPDQK PSWFTPSVNL LANRVMTNIN GAAAASSVTL TSLVLLASEQ 550 560 570 580 590 600 NALERSQLER QLDLYLALLK TVPYTEYASV AEGNGKSIVD HCLSLNKFVS TKDLIGEIIS 610 620 630 640 650 660 VDEKIAITMS YYRNNIIHLM ALPSLIASCL VHYDVCDRQR IHAIVMDFYP LLKAELFMSI 670 680 690 700 710 720 DDVPKHVDCI LDFMVEQGLL TGSDQFEITP RHITQVLLLA ETISETLQRY AIIFNLLAIK 730 740 750 760 770 780 PDLERSELER DSHLLAQRLG ALHGITAPEF YDKKLYNTLS VKLKELGYLC SDEHRAEVIR 790 800 IRDNANKLLS SLVRQTIVDS VEAEHGQ

References

[1]

"Complete sequence of Shewanella frigidimarina NCIMB 400." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCIMB 400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000447 Genomic DNA. Translation: ABI70124.1.
RefSeq	YP_748962.1. NC_008345.1.
3D structure databases
ProteinModelPortal	Q089E1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q089E1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4277032.
GenomeReviews	Gene locus Sfri_0261 in contig CP000447_GR.
KEGG	sfr:Sfri_0261.
NMPDR	fig|318167.10.peg.250.
PATRIC	23494285. VBISheFri14343_0273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHEFN

Accession

Primary (citable) accession number: Q089E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program