Reviewed, UniProtKB/Swiss-Prot Q08987 (FDH2_YEAST)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase 2
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase 2

Gene names

Name:	FDH2
Ordered Locus Names:	YPL275W/YPL276W
ORF Names:	P0323/P0326

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subcellular location	Cytoplasm. Ref.3
Induction	Induced by formate. Ref.3
Miscellaneous	Strains BY4741, S288c and W303 have a stop codon in position 146, which disrupts the gene coding for this protein and produces two ORFs.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	formate catabolic process Ref.3 Inferred from genetic interaction. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Ref.3 Inferred from genetic interaction. Source: SGD oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Formate dehydrogenase 2		PRO_0000223648
Sites
	Active site	272	1	By similarity
	Active site	325	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08987-1 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 67ECDA6F921316FE	FASTA	376	41,736
	10 20 30 40 50 60 MSKGKVLLVL YEGGKHAEEQ EKLLGCIENE LGIRNFIEEQ GYELVTTIDK DPEPTSTVDR 70 80 90 100 110 120 ELKDAEIVIT TPFFPAYISR NRIAEAPNLK LCVTAGVGSD HVDLEAANER KITVTEVTGS 130 140 150 160 170 180 NVVSVAEHVM ATILVLIRNY NGGHQQAING EWDIAGVAKN EYDLEDKIIS TVGAGRIGYR 190 200 210 220 230 240 VLERLVAFNP KKLLYYDYQE LPAEAINRLN EASKLFNGRG DIVQRVEKLE DMVAQSDVVT 250 260 270 280 290 300 INCPLHKDSR GLFNKKLISH MKDGAYLVNT ARGAICVAED VAEAVKSGKL AGYGGDVWDK 310 320 330 340 350 360 QPAPKDHPWR TMDNKDHVGN AMTVHISGTS LHAQKRYAQG VKNILNSYFS KKFDYRPQDI 370 IVQNGSYATR AYGQKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-145. Strain: ATCC 204508 / S288c.
[3]	"Functional analysis of structural genes for NAD(+)-dependent formate dehydrogenase in Saccharomyces cerevisiae." Overkamp K.M., Koetter P., van der Hoek R., Schoondermark-Stolk S., Luttik M.A.H., van Dijken J.P., Pronk J.T. Yeast 19:509-520(2002) [PubMed: 11921099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 93-204, FUNCTION, SUBCELLULAR LOCATION, INDUCTION. Strain: ATCC 200060 / W303, ATCC 201388 / BY4741 and CEN.PK 113-7D.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73632 Genomic DNA. Translation: CAA98012.1. Sequence problems. Z73632 Genomic DNA. Translation: CAA98013.1. Sequence problems. AY558055 Genomic DNA. Translation: AAS56381.1. Sequence problems.
PIR	S65308. S65309.
3D structure databases
HSSP	HSSP built from PDB template 2NAD based on UniProtKB P33160.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3921N.
IntAct	Q08987. 2 interactions.
Genome annotation databases
Ensembl	YOR388C. Saccharomyces cerevisiae. [Contig view]
Organism-specific databases
CYGD	YPL275w. YPL276w.
SGD	S000006196. FDH2. S000006197. YPL276W.
Enzyme and pathway databases
BRENDA	1.2.1.2. 250.
Gene expression databases
GermOnline	YOR388C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH2_YEAST

Accession

Primary (citable) accession number: Q08987
Secondary accession number(s): Q08988

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 21, 2006
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)