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Protein

Homocysteine S-methyltransferase 2

Gene

SAM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Homocysteine S-methyltransferase involved in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio. Converts also S-methylmethionine (SMM) to methionine.1 Publication

Catalytic activityi

S-methyl-L-methionine + L-homocysteine = 2 L-methionine.

Cofactori

Zn2+PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi239 – 2391ZincPROSITE-ProRule annotation
Metal bindingi306 – 3061ZincPROSITE-ProRule annotation
Metal bindingi307 – 3071ZincPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • homocysteine metabolic process Source: SGD
  • L-methionine biosynthetic process from S-adenosylmethionine Source: SGD
  • S-adenosylhomocysteine metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BioCyciYEAST:YPL273W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocysteine S-methyltransferase 2 (EC:2.1.1.10)
Alternative name(s):
S-adenosylmethionine metabolism protein 4
S-methylmethionine:homocysteine methyltransferase 2
Short name:
SMM:Hcy S-methyltransferase 2
Gene namesi
Name:SAM4
Ordered Locus Names:YPL273W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL273W.
SGDiS000006194. SAM4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Homocysteine S-methyltransferase 2PRO_0000114619Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei138 – 1381PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08985.

PTM databases

iPTMnetiQ08985.

Expressioni

Inductioni

Up-regulated in response to high extracellular methionine.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi35940. 13 interactions.
MINTiMINT-4505729.

Structurei

3D structure databases

ProteinModelPortaliQ08985.
SMRiQ08985. Positions 15-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 321316Hcy-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Hcy-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000131604.
HOGENOMiHOG000066018.
InParanoidiQ08985.
KOiK00547.
OMAiSVDGYME.
OrthoDBiEOG092C3PBH.

Family and domain databases

Gene3Di3.20.20.330. 1 hit.
InterProiIPR003726. HCY_dom.
[Graphical view]
PfamiPF02574. S-methyl_trans. 1 hit.
[Graphical view]
SUPFAMiSSF82282. SSF82282. 1 hit.
PROSITEiPS50970. HCY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLPLKQFL ADNPKKVLVL DGGQGTELEN RGIKVANPVW STIPFISESF
60 70 80 90 100
WSDESSANRK IVKEMFNDFL NAGAEILMTT TYQTSYKSVS ENTPIRTLSE
110 120 130 140 150
YNNLLNRIVD FSRNCIGEDK YLIGCIGPWG AHICREFTGD YGAEPENIDF
160 170 180 190 200
YQYFKPQLEN FNKNDKLDLI GFETIPNIHE LKAILSWDES ILSRPFYIGL
210 220 230 240 250
SVHEHGVLRD GTTMEEIAQV IKDLGDKINP NFSFLGINCV SFNQSPDILE
260 270 280 290 300
SLHQALPNMA LLAYPNSGEV YDTEKKIWLP NSDKLNSWDT VVKQYISSGA
310 320
RIIGGCCRTS PKDIQEISAA VKKYT
Length:325
Mass (Da):36,669
Last modified:November 1, 1996 - v1
Checksum:i54658C7B92A610F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73629 Genomic DNA. Translation: CAA98009.1.
BK006949 Genomic DNA. Translation: DAA11163.1.
PIRiS65306.
RefSeqiNP_015050.1. NM_001184087.1.

Genome annotation databases

EnsemblFungiiYPL273W; YPL273W; YPL273W.
GeneIDi855855.
KEGGisce:YPL273W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73629 Genomic DNA. Translation: CAA98009.1.
BK006949 Genomic DNA. Translation: DAA11163.1.
PIRiS65306.
RefSeqiNP_015050.1. NM_001184087.1.

3D structure databases

ProteinModelPortaliQ08985.
SMRiQ08985. Positions 15-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35940. 13 interactions.
MINTiMINT-4505729.

PTM databases

iPTMnetiQ08985.

Proteomic databases

MaxQBiQ08985.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL273W; YPL273W; YPL273W.
GeneIDi855855.
KEGGisce:YPL273W.

Organism-specific databases

EuPathDBiFungiDB:YPL273W.
SGDiS000006194. SAM4.

Phylogenomic databases

GeneTreeiENSGT00840000131604.
HOGENOMiHOG000066018.
InParanoidiQ08985.
KOiK00547.
OMAiSVDGYME.
OrthoDBiEOG092C3PBH.

Enzyme and pathway databases

BioCyciYEAST:YPL273W-MONOMER.

Miscellaneous databases

PROiQ08985.

Family and domain databases

Gene3Di3.20.20.330. 1 hit.
InterProiIPR003726. HCY_dom.
[Graphical view]
PfamiPF02574. S-methyl_trans. 1 hit.
[Graphical view]
SUPFAMiSSF82282. SSF82282. 1 hit.
PROSITEiPS50970. HCY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAM4_YEAST
AccessioniPrimary (citable) accession number: Q08985
Secondary accession number(s): D6W397
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 60300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.