Q08981 (ACM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
APC/C-CDH1 modulator 1

Gene names

Name:	ACM1
Ordered Locus Names:	YPL267W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of GDH1, the activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C), and which is required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Ref.6
Subunit structure	Interacts with CDH1, BMH1 and BMH2. Ref.6
Induction	Expressed very quickly after G1 release, just prior to initiation of DNA replication. Ref.6
Miscellaneous	Present with 688 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Transcription Transcription regulation
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Inferred from direct assay. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ubiquitin-protein ligase inhibitor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	APC/C-CDH1 modulator 1		PRO_0000268174
Amino acid modifications
	Modified residue	37	1	Phosphoserine Ref.7
	Modified residue	41	1	Phosphothreonine Ref.7
	Modified residue	48	1	Phosphoserine Ref.7
	Modified residue	126	1	Phosphoserine Ref.9
	Modified residue	161	1	Phosphothreonine Ref.7 Ref.8 Ref.9
	Modified residue	186	1	Phosphoserine Ref.8
	Modified residue	188	1	Phosphoserine Ref.7 Ref.9
	Modified residue	189	1	Phosphothreonine Ref.8
	Modified residue	202	1	Phosphoserine Ref.5 Ref.7 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q08981 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0646D4E3AD41758C	FASTA	209	23,870
	10 20 30 40 50 60 MISPSKKRTI LSSKNINQKP RAVVKGNELR SPSKRRSQID TDYALRRSPI KTIQISKAAQ 70 80 90 100 110 120 FMLYEETAEE RNIAVHRHNE IYNNNNSVSN ENNPSQVKEN LSPAKICPYE RAFLREGGRI 130 140 150 160 170 180 ALKDLSVDEF KGYIQDPLTD ETIPLTLPLG DKKISLPSFI TPPRNSKISI FFTSKHQGQN 190 200 PETKISRSTD DVSEKKVVRK LSFHVYEDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Acm1 is a negative regulator of the CDH1-dependent anaphase-promoting complex/cyclosome in budding yeast." Martinez J.S., Jeong D.-E., Choi E., Billings B.M., Hall M.C. Mol. Cell. Biol. 26:9162-9176(2006) [PubMed: 17030612] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, INDUCTION, INTERACTION WITH CDH1; BMH1 AND BMH2.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37; THR-41; SER-48; THR-161; SER-188 AND SER-202, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161; SER-186 AND THR-189, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126; THR-161; SER-188 AND SER-202, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73623 Genomic DNA. Translation: CAA98002.1. AY558139 Genomic DNA. Translation: AAS56465.1. BK006949 Genomic DNA. Translation: DAA11169.1.
PIR	S65300.
RefSeq	NP_015056.1. NM_001184081.1.
3D structure databases
ProteinModelPortal	Q08981.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8871N.
IntAct	Q08981. 4 interactions.
STRING	Q08981.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL267W; YPL267W; YPL267W.
GeneID	855861.
KEGG	sce:YPL267W.
NMPDR	fig|4932.3.peg.6180.
Organism-specific databases
CYGD	YPL267w.
SGD	S000006188. ACM1.
Phylogenomic databases
eggNOG	fuNOG12788.
OMA	HETHVND.
OrthoDB	EOG4ZCXF8.
Gene expression databases
ArrayExpress	Q08981.
Genevestigator	Q08981.
GermOnline	YPL267W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	980478.

Entry information

Entry name

ACM1_YEAST

Accession

Primary (citable) accession number: Q08981
Secondary accession number(s): D6W3A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program