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Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21

Gene

THI21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probably that of HMP to HMP-P.1 Publication

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-2-methyl-5-(phosphomethyl)pyrimidine.Curated
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine.Curated

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (THI21), Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (THI20)
  3. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (THI21), Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (THI20)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydroxymethylpyrimidine kinase activity Source: SGD
  • phosphomethylpyrimidine kinase activity Source: SGD
  • thiaminase activity Source: InterPro

GO - Biological processi

  • thiamine biosynthetic process Source: SGD
  • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-81
YEAST:MONOMER3O-81
UniPathwayiUPA00060; UER00137
UPA00060; UER00138

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (EC:2.7.1.49Curated, EC:2.7.4.7Curated)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:THI21
Ordered Locus Names:YPL258C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL258C
SGDiS000006179 THI21

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001920421 – 551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21Add BLAST551

Proteomic databases

PaxDbiQ08975
PRIDEiQ08975

Expressioni

Inductioni

By absence of thiamine.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399403EBI-30327,EBI-16219

Protein-protein interaction databases

BioGridi35954, 51 interactors
DIPiDIP-5635N
IntActiQ08975, 41 interactors
MINTiQ08975
STRINGi4932.YPL258C

Structurei

3D structure databases

ProteinModelPortaliQ08975
SMRiQ08975
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the thiaminase-2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003953
HOGENOMiHOG000225275
InParanoidiQ08975
KOiK00877
OMAiQIATHEN
OrthoDBiEOG092C1B4A

Family and domain databases

CDDicd01169 HMPP_kinase, 1 hit
Gene3Di1.20.910.10, 1 hit
3.40.1190.20, 1 hit
InterProiView protein in InterPro
IPR016084 Haem_Oase-like_multi-hlx
IPR004399 HMP/HMP-P_kinase
IPR013749 PM/HMP-P_kinase-1
IPR029056 Ribokinase-like
IPR004305 Thiaminase-2/PQQC
IPR027574 Thiaminase_II
PfamiView protein in Pfam
PF08543 Phos_pyr_kin, 1 hit
PF03070 TENA_THI-4, 1 hit
SUPFAMiSSF48613 SSF48613, 1 hit
SSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00097 HMP-P_kinase, 1 hit
TIGR04306 salvage_TenA, 1 hit

Sequencei

Sequence statusi: Complete.

Q08975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYSTVNINT PPPYLALASN EKLPTVLSIA GTDPSGGAGV EADVKTITAH
60 70 80 90 100
RCYAMTCITA LNAQTPVKVY SINNTPKEVV SQILDANLQD MKCDVIKTGM
110 120 130 140 150
LTTAAIEVLH EKLLQLGENR PKLVVDPVLV ATSGSSLAGK DIASLITEKI
160 170 180 190 200
APFADILTPN IPECFKLLGE DREISKLRDI FEVAKDLAKI TKCSNILVKG
210 220 230 240 250
GHIPWNDEEG KYITDVLYLG AEQRFITFKG NFVNTTHTHG TGCTLASAIA
260 270 280 290 300
SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAIEIP
310 320 330 340 350
LEKMLSDECF TASDAVHKKP VKSSLNKIPG GSFYKYLINH PKVKPHWDSY
360 370 380 390 400
VNHDFVRKVA DGSLEPKKFQ FFIEQDYLYL VNYARISCIA GSKSPCLEDL
410 420 430 440 450
EKELVIVECV RNGLCQHERR LREEFGIKDP DYLQKIQRGP ALRAYCRYFN
460 470 480 490 500
DVSRRGNWQE LVIALNPCLM GYVHALTKIK DEVTAAEGSV YREWCETYSS
510 520 530 540 550
SWCHEAMLEG EKLLNHILET YPPEKLDTLV TIYAEVCELE ANFWTAALEY

E
Length:551
Mass (Da):61,334
Last modified:November 1, 1996 - v1
Checksum:iB8FD8D7DF6A25961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73614 Genomic DNA Translation: CAA97986.1
BK006949 Genomic DNA Translation: DAA11177.1
PIRiS65289
RefSeqiNP_015065.1, NM_001184072.1

Genome annotation databases

EnsemblFungiiYPL258C; YPL258C; YPL258C
GeneIDi855870
KEGGisce:YPL258C

Similar proteinsi

Entry informationi

Entry nameiTHI21_YEAST
AccessioniPrimary (citable) accession number: Q08975
Secondary accession number(s): D6W3B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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