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Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21

Gene

THI21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probably that of HMP to HMP-P.1 Publication

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: SGD
  3. phosphomethylpyrimidine kinase activity Source: SGD
  4. thiaminase activity Source: InterPro

GO - Biological processi

  1. thiamine biosynthetic process Source: SGD
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-81.
YEAST:MONOMER3O-81.
UniPathwayiUPA00060; UER00137.
UPA00060; UER00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:THI21
Ordered Locus Names:YPL258C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL258c.
SGDiS000006179. THI21.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21PRO_0000192042Add
BLAST

Proteomic databases

PaxDbiQ08975.

Expressioni

Inductioni

By absence of thiamine.1 Publication

Gene expression databases

GenevestigatoriQ08975.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399401EBI-30327,EBI-16219

Protein-protein interaction databases

BioGridi35954. 16 interactions.
DIPiDIP-5635N.
IntActiQ08975. 2 interactions.
MINTiMINT-522674.
STRINGi4932.YPL258C.

Structurei

3D structure databases

ProteinModelPortaliQ08975.
SMRiQ08975. Positions 2-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the thiaminase-2 family.Curated

Phylogenomic databases

eggNOGiCOG0351.
GeneTreeiENSGT00390000003953.
HOGENOMiHOG000225275.
InParanoidiQ08975.
KOiK00877.
OMAiARISCIA.
OrthoDBiEOG75MW5D.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
3.40.1190.20. 1 hit.
InterProiIPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR027574. Thiaminase_II.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR04306. salvage_TenA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08975-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTYSTVNINT PPPYLALASN EKLPTVLSIA GTDPSGGAGV EADVKTITAH
60 70 80 90 100
RCYAMTCITA LNAQTPVKVY SINNTPKEVV SQILDANLQD MKCDVIKTGM
110 120 130 140 150
LTTAAIEVLH EKLLQLGENR PKLVVDPVLV ATSGSSLAGK DIASLITEKI
160 170 180 190 200
APFADILTPN IPECFKLLGE DREISKLRDI FEVAKDLAKI TKCSNILVKG
210 220 230 240 250
GHIPWNDEEG KYITDVLYLG AEQRFITFKG NFVNTTHTHG TGCTLASAIA
260 270 280 290 300
SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAIEIP
310 320 330 340 350
LEKMLSDECF TASDAVHKKP VKSSLNKIPG GSFYKYLINH PKVKPHWDSY
360 370 380 390 400
VNHDFVRKVA DGSLEPKKFQ FFIEQDYLYL VNYARISCIA GSKSPCLEDL
410 420 430 440 450
EKELVIVECV RNGLCQHERR LREEFGIKDP DYLQKIQRGP ALRAYCRYFN
460 470 480 490 500
DVSRRGNWQE LVIALNPCLM GYVHALTKIK DEVTAAEGSV YREWCETYSS
510 520 530 540 550
SWCHEAMLEG EKLLNHILET YPPEKLDTLV TIYAEVCELE ANFWTAALEY

E
Length:551
Mass (Da):61,334
Last modified:November 1, 1996 - v1
Checksum:iB8FD8D7DF6A25961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73614 Genomic DNA. Translation: CAA97986.1.
BK006949 Genomic DNA. Translation: DAA11177.1.
PIRiS65289.
RefSeqiNP_015065.1. NM_001184072.1.

Genome annotation databases

EnsemblFungiiYPL258C; YPL258C; YPL258C.
GeneIDi855870.
KEGGisce:YPL258C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73614 Genomic DNA. Translation: CAA97986.1.
BK006949 Genomic DNA. Translation: DAA11177.1.
PIRiS65289.
RefSeqiNP_015065.1. NM_001184072.1.

3D structure databases

ProteinModelPortaliQ08975.
SMRiQ08975. Positions 2-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35954. 16 interactions.
DIPiDIP-5635N.
IntActiQ08975. 2 interactions.
MINTiMINT-522674.
STRINGi4932.YPL258C.

Proteomic databases

PaxDbiQ08975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL258C; YPL258C; YPL258C.
GeneIDi855870.
KEGGisce:YPL258C.

Organism-specific databases

CYGDiYPL258c.
SGDiS000006179. THI21.

Phylogenomic databases

eggNOGiCOG0351.
GeneTreeiENSGT00390000003953.
HOGENOMiHOG000225275.
InParanoidiQ08975.
KOiK00877.
OMAiARISCIA.
OrthoDBiEOG75MW5D.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00137.
UPA00060; UER00138.
BioCyciMetaCyc:MONOMER3O-81.
YEAST:MONOMER3O-81.

Miscellaneous databases

NextBioi980502.

Gene expression databases

GenevestigatoriQ08975.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
3.40.1190.20. 1 hit.
InterProiIPR016084. Haem_Oase-like_multi-hlx.
IPR004399. HMP/HMP-P_kinase.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR027574. Thiaminase_II.
[Graphical view]
PfamiPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
TIGR04306. salvage_TenA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway."
    Llorente B., Fairhead C., Dujon B.
    Mol. Microbiol. 32:1140-1152(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A HMPP KINASE, INDUCTION.

Entry informationi

Entry nameiTHI21_YEAST
AccessioniPrimary (citable) accession number: Q08975
Secondary accession number(s): D6W3B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.