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Q08975

- THI21_YEAST

UniProt

Q08975 - THI21_YEAST

Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21

Gene

THI21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probably that of HMP to HMP-P.1 Publication

    Catalytic activityi

    ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
    ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydroxymethylpyrimidine kinase activity Source: SGD
    3. phosphomethylpyrimidine kinase activity Source: SGD
    4. thiaminase activity Source: InterPro

    GO - Biological processi

    1. thiamine biosynthetic process Source: SGD
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-81.
    YEAST:MONOMER3O-81.
    UniPathwayiUPA00060; UER00137.
    UPA00060; UER00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (EC:2.7.1.49, EC:2.7.4.7)
    Alternative name(s):
    Hydroxymethylpyrimidine kinase
    Short name:
    HMP kinase
    Hydroxymethylpyrimidine phosphate kinase
    Short name:
    HMP-P kinase
    Short name:
    HMP-phosphate kinase
    Short name:
    HMPP kinase
    Gene namesi
    Name:THI21
    Ordered Locus Names:YPL258C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL258c.
    SGDiS000006179. THI21.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21PRO_0000192042Add
    BLAST

    Proteomic databases

    PaxDbiQ08975.

    Expressioni

    Inductioni

    By absence of thiamine.1 Publication

    Gene expression databases

    GenevestigatoriQ08975.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399401EBI-30327,EBI-16219

    Protein-protein interaction databases

    BioGridi35954. 16 interactions.
    DIPiDIP-5635N.
    IntActiQ08975. 2 interactions.
    MINTiMINT-522674.
    STRINGi4932.YPL258C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08975.
    SMRiQ08975. Positions 2-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    In the N-terminal section; belongs to the ThiD family.Curated
    In the C-terminal section; belongs to the thiaminase-2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0351.
    GeneTreeiENSGT00390000003953.
    HOGENOMiHOG000225275.
    KOiK00877.
    OMAiARISCIA.
    OrthoDBiEOG75MW5D.

    Family and domain databases

    Gene3Di1.20.910.10. 1 hit.
    3.40.1190.20. 1 hit.
    InterProiIPR016084. Haem_Oase-like_multi-hlx.
    IPR004399. HMP/HMP-P_kinase.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    IPR004305. Thiaminase-2/PQQC.
    IPR027574. Thiaminase_II.
    [Graphical view]
    PfamiPF08543. Phos_pyr_kin. 1 hit.
    PF03070. TENA_THI-4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48613. SSF48613. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
    TIGR04306. salvage_TenA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08975-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTYSTVNINT PPPYLALASN EKLPTVLSIA GTDPSGGAGV EADVKTITAH    50
    RCYAMTCITA LNAQTPVKVY SINNTPKEVV SQILDANLQD MKCDVIKTGM 100
    LTTAAIEVLH EKLLQLGENR PKLVVDPVLV ATSGSSLAGK DIASLITEKI 150
    APFADILTPN IPECFKLLGE DREISKLRDI FEVAKDLAKI TKCSNILVKG 200
    GHIPWNDEEG KYITDVLYLG AEQRFITFKG NFVNTTHTHG TGCTLASAIA 250
    SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAIEIP 300
    LEKMLSDECF TASDAVHKKP VKSSLNKIPG GSFYKYLINH PKVKPHWDSY 350
    VNHDFVRKVA DGSLEPKKFQ FFIEQDYLYL VNYARISCIA GSKSPCLEDL 400
    EKELVIVECV RNGLCQHERR LREEFGIKDP DYLQKIQRGP ALRAYCRYFN 450
    DVSRRGNWQE LVIALNPCLM GYVHALTKIK DEVTAAEGSV YREWCETYSS 500
    SWCHEAMLEG EKLLNHILET YPPEKLDTLV TIYAEVCELE ANFWTAALEY 550
    E 551
    Length:551
    Mass (Da):61,334
    Last modified:November 1, 1996 - v1
    Checksum:iB8FD8D7DF6A25961
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73614 Genomic DNA. Translation: CAA97986.1.
    BK006949 Genomic DNA. Translation: DAA11177.1.
    PIRiS65289.
    RefSeqiNP_015065.1. NM_001184072.1.

    Genome annotation databases

    EnsemblFungiiYPL258C; YPL258C; YPL258C.
    GeneIDi855870.
    KEGGisce:YPL258C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73614 Genomic DNA. Translation: CAA97986.1 .
    BK006949 Genomic DNA. Translation: DAA11177.1 .
    PIRi S65289.
    RefSeqi NP_015065.1. NM_001184072.1.

    3D structure databases

    ProteinModelPortali Q08975.
    SMRi Q08975. Positions 2-551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35954. 16 interactions.
    DIPi DIP-5635N.
    IntActi Q08975. 2 interactions.
    MINTi MINT-522674.
    STRINGi 4932.YPL258C.

    Proteomic databases

    PaxDbi Q08975.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL258C ; YPL258C ; YPL258C .
    GeneIDi 855870.
    KEGGi sce:YPL258C.

    Organism-specific databases

    CYGDi YPL258c.
    SGDi S000006179. THI21.

    Phylogenomic databases

    eggNOGi COG0351.
    GeneTreei ENSGT00390000003953.
    HOGENOMi HOG000225275.
    KOi K00877.
    OMAi ARISCIA.
    OrthoDBi EOG75MW5D.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00137 .
    UPA00060 ; UER00138 .
    BioCyci MetaCyc:MONOMER3O-81.
    YEAST:MONOMER3O-81.

    Miscellaneous databases

    NextBioi 980502.

    Gene expression databases

    Genevestigatori Q08975.

    Family and domain databases

    Gene3Di 1.20.910.10. 1 hit.
    3.40.1190.20. 1 hit.
    InterProi IPR016084. Haem_Oase-like_multi-hlx.
    IPR004399. HMP/HMP-P_kinase.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    IPR004305. Thiaminase-2/PQQC.
    IPR027574. Thiaminase_II.
    [Graphical view ]
    Pfami PF08543. Phos_pyr_kin. 1 hit.
    PF03070. TENA_THI-4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48613. SSF48613. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00097. HMP-P_kinase. 1 hit.
    TIGR04306. salvage_TenA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway."
      Llorente B., Fairhead C., Dujon B.
      Mol. Microbiol. 32:1140-1152(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A HMPP KINASE, INDUCTION.

    Entry informationi

    Entry nameiTHI21_YEAST
    AccessioniPrimary (citable) accession number: Q08975
    Secondary accession number(s): D6W3B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3