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Q08975 (THI21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21

EC=2.7.1.49
EC=2.7.4.7
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name=HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name=HMP-P kinase
Short name=HMP-phosphate kinase
Short name=HMPP kinase
Gene names
Name:THI21
Ordered Locus Names:YPL258C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probaby that of HMP to HMP-P. Ref.3

Catalytic activity

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.

ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3.

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.

Induction

By absence of thiamine. Ref.3

Sequence similarities

In the N-terminal section; belongs to the ThiD family.

In the C-terminal section; belongs to the thiaminase-2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSP5P399401EBI-30327,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21
PRO_0000192042

Sites

Binding site641Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08975 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B8FD8D7DF6A25961

FASTA55161,334
        10         20         30         40         50         60 
MTYSTVNINT PPPYLALASN EKLPTVLSIA GTDPSGGAGV EADVKTITAH RCYAMTCITA 

        70         80         90        100        110        120 
LNAQTPVKVY SINNTPKEVV SQILDANLQD MKCDVIKTGM LTTAAIEVLH EKLLQLGENR 

       130        140        150        160        170        180 
PKLVVDPVLV ATSGSSLAGK DIASLITEKI APFADILTPN IPECFKLLGE DREISKLRDI 

       190        200        210        220        230        240 
FEVAKDLAKI TKCSNILVKG GHIPWNDEEG KYITDVLYLG AEQRFITFKG NFVNTTHTHG 

       250        260        270        280        290        300 
TGCTLASAIA SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAIEIP 

       310        320        330        340        350        360 
LEKMLSDECF TASDAVHKKP VKSSLNKIPG GSFYKYLINH PKVKPHWDSY VNHDFVRKVA 

       370        380        390        400        410        420 
DGSLEPKKFQ FFIEQDYLYL VNYARISCIA GSKSPCLEDL EKELVIVECV RNGLCQHERR 

       430        440        450        460        470        480 
LREEFGIKDP DYLQKIQRGP ALRAYCRYFN DVSRRGNWQE LVIALNPCLM GYVHALTKIK 

       490        500        510        520        530        540 
DEVTAAEGSV YREWCETYSS SWCHEAMLEG EKLLNHILET YPPEKLDTLV TIYAEVCELE 

       550 
ANFWTAALEY E 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway."
Llorente B., Fairhead C., Dujon B.
Mol. Microbiol. 32:1140-1152(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A HMPP KINASE, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73614 Genomic DNA. Translation: CAA97986.1.
BK006949 Genomic DNA. Translation: DAA11177.1.
PIRS65289.
RefSeqNP_015065.1. NM_001184072.1.

3D structure databases

ProteinModelPortalQ08975.
SMRQ08975. Positions 2-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35954. 16 interactions.
DIPDIP-5635N.
IntActQ08975. 2 interactions.
MINTMINT-522674.
STRING4932.YPL258C.

Proteomic databases

PaxDbQ08975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL258C; YPL258C; YPL258C.
GeneID855870.
KEGGsce:YPL258C.

Organism-specific databases

CYGDYPL258c.
SGDS000006179. THI21.

Phylogenomic databases

eggNOGCOG0351.
GeneTreeENSGT00390000003953.
HOGENOMHOG000225275.
KOK00877.
OMAARISCIA.
OrthoDBEOG75MW5D.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER3O-81.
YEAST:MONOMER3O-81.
UniPathwayUPA00060; UER00137.
UPA00060; UER00138.

Gene expression databases

GenevestigatorQ08975.

Family and domain databases

Gene3D1.20.910.10. 1 hit.
InterProIPR016084. Haem_Oase-like_multi-hlx.
IPR013749. HMP-P_kinase-1.
IPR004399. HMP-P_kinase-2.
IPR004305. Thiaminase-2/PQQC.
IPR027574. Thiaminase_II.
[Graphical view]
PfamPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMSSF48613. SSF48613. 1 hit.
TIGRFAMsTIGR00097. HMP-P_kinase. 1 hit.
TIGR04306. salvage_TenA. 1 hit.
ProtoNetSearch...

Other

NextBio980502.

Entry information

Entry nameTHI21_YEAST
AccessionPrimary (citable) accession number: Q08975
Secondary accession number(s): D6W3B1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways