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Protein

[NU+] prion formation protein 1

Gene

NEW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the mRNA export process (By similarity). Forms the [NU+] prion and induces [PSI+] prion formation.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi604 – 6118ATP 1PROSITE-ProRule annotation
Nucleotide bindingi846 – 8538ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: SGD

GO - Biological processi

  • poly(A)+ mRNA export from nucleus Source: SGD
  • ribosomal small subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Prion

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34115-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
[NU+] prion formation protein 1
Gene namesi
Name:NEW1
Ordered Locus Names:YPL226W
ORF Names:P1445
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL226W.
SGDiS000006147. NEW1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
  • polysome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11961196[NU+] prion formation protein 1PRO_0000268690Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei443 – 4431PhosphoserineCombined sources
Modified residuei1191 – 11911PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08972.
PeptideAtlasiQ08972.

PTM databases

iPTMnetiQ08972.

Interactioni

Protein-protein interaction databases

BioGridi35959. 241 interactions.
DIPiDIP-6323N.
IntActiQ08972. 29 interactions.
MINTiMINT-640344.

Structurei

3D structure databases

ProteinModelPortaliQ08972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini570 – 786217ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini812 – 1129318ABC transporter 2PROSITE-ProRule annotationAdd
BLAST
Domaini942 – 100362ChromoPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi61 – 10444Tyr-richAdd
BLAST
Compositional biasi70 – 9526Asn-richAdd
BLAST

Domaini

The N-terminal 153 residues are responsible for the prion properties of NEW1.2 Publications

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation
Contains 1 chromo domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075671.
HOGENOMiHOG000180957.
InParanoidiQ08972.
KOiK03235.
OMAiPKGTPHP.
OrthoDBiEOG7Q2NDN.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF00385. Chromo. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS50013. CHROMO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKKFKDLN SFLDDQPKDP NLVASPFGGY FKNPAADAGS NNASKKSSYQ
60 70 80 90 100
QQRNWKQGGN YQQGGYQSYN SNYNNYNNYN NYNNYNNYNN YNKYNGQGYQ
110 120 130 140 150
KSTYKQSAVT PNQSGTPTPS ASTTSLTSLN EKLSNLELTP ISQFLSKIPE
160 170 180 190 200
CQSITDCKNQ IKLIIEEFGK EGNSTGEKIE EWKIVDVLSK FIKPKNPSLV
210 220 230 240 250
RESAMLIISN IAQFFSGKPP QEAYLLPFFN VALDCISDKE NTVKRAAQHA
260 270 280 290 300
IDSLLNCFPM EALTCFVLPT ILDYLSSGAK WQAKMAALSV VDRIREDSAN
310 320 330 340 350
DLLELTFKDA VPVLTDVATD FKPELAKQGY KTLLDYVSIL DNLDLSPRYK
360 370 380 390 400
LIVDTLQDPS KVPESVKSLS SVTFVAEVTE PSLSLLVPIL NRSLNLSSSS
410 420 430 440 450
QEQLRQTVIV VENLTRLVNN RNEIESFIPL LLPGIQKVVD TASLPEVREL
460 470 480 490 500
AEKALNVLKE DDEADKENKF SGRLTLEEGR DFLLDHLKDI KADDSCFVKP
510 520 530 540 550
YMNDETVIKY MSKILTVDSN VNDWKRLEDF LTAVFGGSDS QREFVKQDFI
560 570 580 590 600
HNLRALFYQE KERADEDEGI EIVNTDFSLA YGSRMLLNKT NLRLLKGHRY
610 620 630 640 650
GLCGRNGAGK STLMRAIANG QLDGFPDKDT LRTCFVEHKL QGEEGDLDLV
660 670 680 690 700
SFIALDEELQ STSREEIAAA LESVGFDEER RAQTVGSLSG GWKMKLELAR
710 720 730 740 750
AMLQKADILL LDEPTNHLDV SNVKWLEEYL LEHTDITSLI VSHDSGFLDT
760 770 780 790 800
VCTDIIHYEN KKLAYYKGNL AAFVEQKPEA KSYYTLTDSN AQMRFPPPGI
810 820 830 840 850
LTGVKSNTRA VAKMTDVTFS YPGAQKPSLS HVSCSLSLSS RVACLGPNGA
860 870 880 890 900
GKSTLIKLLT GELVPNEGKV EKHPNLRIGY IAQHALQHVN EHKEKTANQY
910 920 930 940 950
LQWRYQFGDD REVLLKESRK ISEDEKEMMT KEIDIDDGRG KRAIEAIVGR
960 970 980 990 1000
QKLKKSFQYE VKWKYWKPKY NSWVPKDVLV EHGFEKLVQK FDDHEASREG
1010 1020 1030 1040 1050
LGYRELIPSV ITKHFEDVGL DSEIANHTPL GSLSGGQLVK VVIAGAMWNN
1060 1070 1080 1090 1100
PHLLVLDEPT NYLDRDSLGA LAVAIRDWSG GVVMISHNNE FVGALCPEQW
1110 1120 1130 1140 1150
IVENGKMVQK GSAQVDQSKF EDGGNADAVG LKASNLAKPS VDDDDSPANI
1160 1170 1180 1190
KVKQRKKRLT RNEKKLQAER RRLRYIEWLS SPKGTPKPVD TDDEED
Length:1,196
Mass (Da):134,331
Last modified:November 1, 1996 - v1
Checksum:i3C8C52A3F54C2902
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94561 Genomic DNA. Translation: CAA64261.1.
Z73582 Genomic DNA. Translation: CAA97941.1.
BK006949 Genomic DNA. Translation: DAA11210.1.
PIRiS65245.
RefSeqiNP_015098.1. NM_001184040.1.

Genome annotation databases

EnsemblFungiiYPL226W; YPL226W; YPL226W.
GeneIDi855875.
KEGGisce:YPL226W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94561 Genomic DNA. Translation: CAA64261.1.
Z73582 Genomic DNA. Translation: CAA97941.1.
BK006949 Genomic DNA. Translation: DAA11210.1.
PIRiS65245.
RefSeqiNP_015098.1. NM_001184040.1.

3D structure databases

ProteinModelPortaliQ08972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35959. 241 interactions.
DIPiDIP-6323N.
IntActiQ08972. 29 interactions.
MINTiMINT-640344.

PTM databases

iPTMnetiQ08972.

Proteomic databases

MaxQBiQ08972.
PeptideAtlasiQ08972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL226W; YPL226W; YPL226W.
GeneIDi855875.
KEGGisce:YPL226W.

Organism-specific databases

EuPathDBiFungiDB:YPL226W.
SGDiS000006147. NEW1.

Phylogenomic databases

GeneTreeiENSGT00550000075671.
HOGENOMiHOG000180957.
InParanoidiQ08972.
KOiK03235.
OMAiPKGTPHP.
OrthoDBiEOG7Q2NDN.

Enzyme and pathway databases

BioCyciYEAST:G3O-34115-MONOMER.

Miscellaneous databases

NextBioi980517.
PROiQ08972.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF00385. Chromo. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS50013. CHROMO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Complete inventory of the yeast ABC proteins."
    Decottignies A., Goffeau A.
    Nat. Genet. 15:137-145(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS, PREDICTION OF FUNCTION.
  4. "Molecular basis of a yeast prion species barrier."
    Santoso A., Chien P., Osherovich L.Z., Weissman J.S.
    Cell 100:277-288(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS A PRION-FORMING PROTEIN.
  5. "Prions affect the appearance of other prions: the story of [PIN(+)]."
    Derkatch I.L., Bradley M.E., Hong J.Y., Liebman S.W.
    Cell 106:171-182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRION FORMATION.
  6. "Multiple Gln/Asn-rich prion domains confer susceptibility to induction of the yeast [PSI(+)] prion."
    Osherovich L.Z., Weissman J.S.
    Cell 106:183-194(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, PRION FORMATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443 AND THR-1191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNEW1_YEAST
AccessioniPrimary (citable) accession number: Q08972
Secondary accession number(s): D6W3E4, Q05402, Q7LHP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 37600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.