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Protein

U2 small nuclear ribonucleoprotein A'

Gene

LEA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. Associates to U2 snRNA in a MSL1 dependent manner and is required for normal accumulation of U2 snRNA. Required for the spliceosome assembly and the efficient addition of U2 snRNP onto the pre-mRNA.1 Publication

Miscellaneous

Present with 3630 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34103-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 small nuclear ribonucleoprotein A'
Short name:
U2 snRNP A'
Alternative name(s):
Looks exceptionally like U2A protein 1
Gene namesi
Name:LEA1
Ordered Locus Names:YPL213W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL213W.
SGDiS000006134. LEA1.

Subcellular locationi

GO - Cellular componenti

  • U2 snRNP Source: SGD
  • U2-type prespliceosome Source: SGD

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741891 – 238U2 small nuclear ribonucleoprotein A'Add BLAST238

Proteomic databases

MaxQBiQ08963.
PRIDEiQ08963.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with MSL1.2 Publications

Protein-protein interaction databases

BioGridi35972. 642 interactors.
DIPiDIP-2399N.
IntActiQ08963. 29 interactors.
MINTiMINT-368955.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 9Combined sources5
Beta strandi13 – 15Combined sources3
Beta strandi34 – 37Combined sources4
Helixi47 – 50Combined sources4
Beta strandi55 – 58Combined sources4
Beta strandi78 – 80Combined sources3
Beta strandi100 – 102Combined sources3
Helixi111 – 119Combined sources9
Beta strandi126 – 128Combined sources3
Helixi133 – 136Combined sources4
Helixi140 – 147Combined sources8
Beta strandi152 – 154Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMKelectron microscopy3.40b1-238[»]
5LJ3electron microscopy3.80W1-238[»]
5LJ5electron microscopy3.80W1-238[»]
5MQ0electron microscopy4.17W1-238[»]
5WSGelectron microscopy4.00Y1-238[»]
ProteinModelPortaliQ08963.
SMRiQ08963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 74LRR 1Add BLAST22
Repeati75 – 95LRR 2Add BLAST21
Repeati97 – 118LRR 3Add BLAST22
Domaini132 – 170LRRCTAdd BLAST39

Sequence similaritiesi

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

HOGENOMiHOG000000976.
KOiK11092.
OMAiKPTHILD.
OrthoDBiEOG092C47XU.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiView protein in InterPro
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
SMARTiView protein in SMART
SM00446. LRRcap. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiView protein in PROSITE
PS51450. LRR. 3 hits.

Sequencei

Sequence statusi: Complete.

Q08963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL
60 70 80 90 100
TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEVD GRLLPMNVQN
110 120 130 140 150
LTLSNNSIRR FEDLQRLRRA PRTLKNLTLI GNQVCHLANY REHVLRLVPH
160 170 180 190 200
LETLDFQNVT AEERKSAMSF PRQADGDTLG PVNTAIRDNG SRDKTMEIMN
210 220 230
LVVSKMTVER RNELKKQLAE ATSLEEIARL EKLLSGGV
Length:238
Mass (Da):27,193
Last modified:November 1, 1996 - v1
Checksum:i0E92A1922BFEDC25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73569 Genomic DNA. Translation: CAA97928.1.
AY558137 Genomic DNA. Translation: AAS56463.1.
BK006949 Genomic DNA. Translation: DAA11223.1.
PIRiS65232.
RefSeqiNP_015111.1. NM_001184027.1.

Genome annotation databases

EnsemblFungiiYPL213W; YPL213W; YPL213W.
GeneIDi855888.
KEGGisce:YPL213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73569 Genomic DNA. Translation: CAA97928.1.
AY558137 Genomic DNA. Translation: AAS56463.1.
BK006949 Genomic DNA. Translation: DAA11223.1.
PIRiS65232.
RefSeqiNP_015111.1. NM_001184027.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMKelectron microscopy3.40b1-238[»]
5LJ3electron microscopy3.80W1-238[»]
5LJ5electron microscopy3.80W1-238[»]
5MQ0electron microscopy4.17W1-238[»]
5WSGelectron microscopy4.00Y1-238[»]
ProteinModelPortaliQ08963.
SMRiQ08963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35972. 642 interactors.
DIPiDIP-2399N.
IntActiQ08963. 29 interactors.
MINTiMINT-368955.

Proteomic databases

MaxQBiQ08963.
PRIDEiQ08963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL213W; YPL213W; YPL213W.
GeneIDi855888.
KEGGisce:YPL213W.

Organism-specific databases

EuPathDBiFungiDB:YPL213W.
SGDiS000006134. LEA1.

Phylogenomic databases

HOGENOMiHOG000000976.
KOiK11092.
OMAiKPTHILD.
OrthoDBiEOG092C47XU.

Enzyme and pathway databases

BioCyciYEAST:G3O-34103-MONOMER.

Miscellaneous databases

PROiPR:Q08963.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiView protein in InterPro
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
SMARTiView protein in SMART
SM00446. LRRcap. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiView protein in PROSITE
PS51450. LRR. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiRU2A_YEAST
AccessioniPrimary (citable) accession number: Q08963
Secondary accession number(s): D6W3F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.