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Protein

U2 small nuclear ribonucleoprotein A'

Gene

LEA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. Associates to U2 snRNA in a MSL1 dependent manner and is required for normal accumulation of U2 snRNA. Required for the spliceosome assembly and the efficient addition of U2 snRNP onto the pre-mRNA.1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34103-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 small nuclear ribonucleoprotein A'
Short name:
U2 snRNP A'
Alternative name(s):
Looks exceptionally like U2A protein 1
Gene namesi
Name:LEA1
Ordered Locus Names:YPL213W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL213W.
SGDiS000006134. LEA1.

Subcellular locationi

GO - Cellular componenti

  • U2 snRNP Source: SGD
  • U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238U2 small nuclear ribonucleoprotein A'PRO_0000074189Add
BLAST

Proteomic databases

MaxQBiQ08963.
PeptideAtlasiQ08963.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with MSL1.2 Publications

Protein-protein interaction databases

BioGridi35972. 291 interactions.
DIPiDIP-2399N.
IntActiQ08963. 29 interactions.
MINTiMINT-368955.

Structurei

3D structure databases

ProteinModelPortaliQ08963.
SMRiQ08963. Positions 1-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 7422LRR 1Add
BLAST
Repeati75 – 9521LRR 2Add
BLAST
Repeati97 – 11822LRR 3Add
BLAST
Domaini132 – 17039LRRCTAdd
BLAST

Sequence similaritiesi

Contains 3 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

HOGENOMiHOG000000976.
KOiK11092.
OMAiKPTHILD.
OrthoDBiEOG73RBQ5.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL
60 70 80 90 100
TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEVD GRLLPMNVQN
110 120 130 140 150
LTLSNNSIRR FEDLQRLRRA PRTLKNLTLI GNQVCHLANY REHVLRLVPH
160 170 180 190 200
LETLDFQNVT AEERKSAMSF PRQADGDTLG PVNTAIRDNG SRDKTMEIMN
210 220 230
LVVSKMTVER RNELKKQLAE ATSLEEIARL EKLLSGGV
Length:238
Mass (Da):27,193
Last modified:November 1, 1996 - v1
Checksum:i0E92A1922BFEDC25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73569 Genomic DNA. Translation: CAA97928.1.
AY558137 Genomic DNA. Translation: AAS56463.1.
BK006949 Genomic DNA. Translation: DAA11223.1.
PIRiS65232.
RefSeqiNP_015111.1. NM_001184027.1.

Genome annotation databases

EnsemblFungiiYPL213W; YPL213W; YPL213W.
GeneIDi855888.
KEGGisce:YPL213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73569 Genomic DNA. Translation: CAA97928.1.
AY558137 Genomic DNA. Translation: AAS56463.1.
BK006949 Genomic DNA. Translation: DAA11223.1.
PIRiS65232.
RefSeqiNP_015111.1. NM_001184027.1.

3D structure databases

ProteinModelPortaliQ08963.
SMRiQ08963. Positions 1-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35972. 291 interactions.
DIPiDIP-2399N.
IntActiQ08963. 29 interactions.
MINTiMINT-368955.

Proteomic databases

MaxQBiQ08963.
PeptideAtlasiQ08963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL213W; YPL213W; YPL213W.
GeneIDi855888.
KEGGisce:YPL213W.

Organism-specific databases

EuPathDBiFungiDB:YPL213W.
SGDiS000006134. LEA1.

Phylogenomic databases

HOGENOMiHOG000000976.
KOiK11092.
OMAiKPTHILD.
OrthoDBiEOG73RBQ5.

Enzyme and pathway databases

BioCyciYEAST:G3O-34103-MONOMER.

Miscellaneous databases

NextBioi980556.
PROiQ08963.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The yeast U2A'/U2B complex is required for pre-spliceosome formation."
    Caspary F., Seraphin B.
    EMBO J. 17:6348-6358(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MSL1.
  5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRU2A_YEAST
AccessioniPrimary (citable) accession number: Q08963
Secondary accession number(s): D6W3F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.