Reviewed, UniProtKB/Swiss-Prot Q08962 (NIP7_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosome subunit biogenesis protein NIP7
Alternative name(s):
    Nuclear import protein 7

Gene names

Name:	NIP7
Ordered Locus Names:	YPL211W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.
Subunit structure	Interacts with NOP8 and RRP43. Interacts with pre-ribosome complex. May bind to RNA. Ref.4 Ref.5
Subcellular location	Cytoplasm. Nucleus › nucleolus.
Miscellaneous	Present with 5170 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the NIP7 family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Traceable author statement. Source: SGD ribosomal large subunit assembly Traceable author statement. Source: SGD
Cellular component	cytosolic large ribosomal subunit Ref.4 Traceable author statement. Source: SGD nucleolus Ref.4 Traceable author statement. Source: SGD preribosome, large subunit precursor Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MBF1	O14467	1	EBI-12067,EBI-2043025
NOP8	Q08287	3	EBI-12067,EBI-12135
RRP43	P25359	3	EBI-12067,EBI-1773

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	60S ribosome subunit biogenesis protein NIP7		PRO_0000218776
Regions
	Domain	94 – 170	77	PUA
Experimental info
	Mutagenesis	161 – 164	4	IVAF → AVAA: Reduces RNA-binding ability in vitro. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q08962-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 82EAE8E4B2FCC31A	FASTA	181	20,381
	10 20 30 40 50 60 MRQLTEEETK VVFEKLAGYI GRNISFLVDN KELPHVFRLQ KDRVYYVPDH VAKLATSVAR 70 80 90 100 110 120 PNLMSLGICL GKFTKTGKFR LHITSLTVLA KHAKYKIWIK PNGEMPFLYG NHVLKAHVGK 130 140 150 160 170 180 MSDDIPEHAG VIVFAMNDVP LGFGVSAKST SESRNMQPTG IVAFRQADIG EYLRDEDTLF T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis." Zanchin N.I.T., Roberts P., DeSilva A., Sherman F., Goldfarb D.S. Mol. Cell. Biol. 17:5001-5015(1997) [PubMed: 9271378] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p." Zanchin N.I.T., Goldfarb D.S. Mol. Cell. Biol. 19:1518-1525(1999) [PubMed: 9891085] [Abstract] Cited for: CHARACTERIZATION, INTERACTION WITH NOP8 AND RRP43.
[5]	"Identification of a 60S preribosomal particle that is closely linked to nuclear export." Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E. Mol. Cell 8:517-529(2001) [PubMed: 11583615] [Abstract] Cited for: INTERACTION WITH PRE-RIBOSOME COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA." Coltri P.P., Guimaraes B.G., Granato D.C., Luz J.S., Teixeira E.C., Oliveira C.C., Zanchin N.I. Biochemistry 46:14177-14187(2007) [PubMed: 18001138] [Abstract] Cited for: RNA-BINDING, MUTAGENESIS OF 161-ILE--PHE-164.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73567 Genomic DNA. Translation: CAA97926.1. AY558059 Genomic DNA. Translation: AAS56385.1.
PIR	S65230.
RefSeq	NP_015113.1.
3D structure databases
SMR	Q08962. Positions 1-176.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1456N.
IntAct	Q08962. 49 interactions.
STRING	Q08962.
Proteomic databases
PeptideAtlas	Q08962.
Genome annotation databases
Ensembl	YPL211W; YPL211W; YPL211W; Saccharomyces cerevisiae. [Genome view]
GeneID	855890.
KEGG	sce:YPL211W.
NMPDR	fig|4932.3.peg.6241.
Organism-specific databases
CYGD	YPL211w.
SGD	S000006132. NIP7.
Phylogenomic databases
eggNOG	fuNOG05650.
HOGENOM	HBG377944.
OMA	NGEMPFL.
OrthoDB	EOG9M0GJ0.
PhylomeDB	Q08962.
Gene expression databases
ArrayExpress	Q08962.
Genevestigator	Q08962.
GermOnline	YPL211W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002478. PUA. IPR015947. PUA-like. IPR016686. Ribosomal_synth_fac_NIP7. [Graphical view]
PIRSF	PIRSF017190. Rbsml_synth_fac_NIP7. 1 hit.
SMART	SM00359. PUA. 1 hit. [Graphical view]
PROSITE	PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980562.

Entry information

Entry name

NIP7_YEAST

Accession

Primary (citable) accession number: Q08962

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)