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Protein

60S ribosome subunit biogenesis protein NIP7

Gene

NIP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

Miscellaneous

Present with 5170 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • ribosome assembly Source: InterPro

Keywordsi

Molecular functionRNA-binding
Biological processRibosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34102-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosome subunit biogenesis protein NIP7
Alternative name(s):
Nuclear import protein 7
Gene namesi
Name:NIP7
Ordered Locus Names:YPL211W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL211W
SGDiS000006132 NIP7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi161 – 164IVAF → AVAA: Reduces RNA-binding ability in vitro. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187761 – 18160S ribosome subunit biogenesis protein NIP7Add BLAST181

Proteomic databases

MaxQBiQ08962
PaxDbiQ08962
PRIDEiQ08962

PTM databases

iPTMnetiQ08962

Interactioni

Subunit structurei

Interacts with NOP8 and RRP43. Interacts with pre-ribosome complex. May bind to RNA.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35974, 335 interactors
DIPiDIP-1456N
IntActiQ08962, 56 interactors
MINTiQ08962
STRINGi4932.YPL211W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6ELZelectron microscopy3.30l1-181[»]
6EM5electron microscopy4.30l1-181[»]
ProteinModelPortaliQ08962
SMRiQ08962
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 170PUAPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the NIP7 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003591
HOGENOMiHOG000168475
InParanoidiQ08962
KOiK07565
OMAiVVKAHVG
OrthoDBiEOG092C4X4T

Family and domain databases

Gene3Di2.30.130.10, 1 hit
InterProiView protein in InterPro
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
IPR005155 Rbsml_synth_fac_NIP7-like
IPR016686 Ribosomal_synth_fac_NIP7
PfamiView protein in Pfam
PF03657 UPF0113, 1 hit
PIRSFiPIRSF017190 Rbsml_synth_fac_NIP7, 1 hit
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF88697 SSF88697, 1 hit
PROSITEiView protein in PROSITE
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q08962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQLTEEETK VVFEKLAGYI GRNISFLVDN KELPHVFRLQ KDRVYYVPDH
60 70 80 90 100
VAKLATSVAR PNLMSLGICL GKFTKTGKFR LHITSLTVLA KHAKYKIWIK
110 120 130 140 150
PNGEMPFLYG NHVLKAHVGK MSDDIPEHAG VIVFAMNDVP LGFGVSAKST
160 170 180
SESRNMQPTG IVAFRQADIG EYLRDEDTLF T
Length:181
Mass (Da):20,381
Last modified:November 1, 1996 - v1
Checksum:i82EAE8E4B2FCC31A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73567 Genomic DNA Translation: CAA97926.1
AY558059 Genomic DNA Translation: AAS56385.1
BK006949 Genomic DNA Translation: DAA11225.1
PIRiS65230
RefSeqiNP_015113.1, NM_001184025.1

Genome annotation databases

EnsemblFungiiYPL211W; YPL211W; YPL211W
GeneIDi855890
KEGGisce:YPL211W

Similar proteinsi

Entry informationi

Entry nameiNIP7_YEAST
AccessioniPrimary (citable) accession number: Q08962
Secondary accession number(s): D6W3F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health