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Protein

60S ribosome subunit biogenesis protein NIP7

Gene

NIP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

GO - Molecular functioni

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • ribosome assembly Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34102-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosome subunit biogenesis protein NIP7
Alternative name(s):
Nuclear import protein 7
Gene namesi
Name:NIP7
Ordered Locus Names:YPL211W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL211W.
SGDiS000006132. NIP7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1644IVAF → AVAA: Reduces RNA-binding ability in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18118160S ribosome subunit biogenesis protein NIP7PRO_0000218776Add
BLAST

Proteomic databases

MaxQBiQ08962.
PeptideAtlasiQ08962.

PTM databases

iPTMnetiQ08962.

Interactioni

Subunit structurei

Interacts with NOP8 and RRP43. Interacts with pre-ribosome complex. May bind to RNA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NOP8Q082873EBI-12067,EBI-12135
RRP43P253594EBI-12067,EBI-1773

Protein-protein interaction databases

BioGridi35974. 108 interactions.
DIPiDIP-1456N.
IntActiQ08962. 46 interactions.
MINTiMINT-400883.

Structurei

3D structure databases

ProteinModelPortaliQ08962.
SMRiQ08962. Positions 1-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 17077PUAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NIP7 family.Curated
Contains 1 PUA domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000003591.
HOGENOMiHOG000168475.
InParanoidiQ08962.
KOiK07565.
OMAiVVKAHVG.
OrthoDBiEOG7K0ZQF.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR005155. Rbsml_synth_fac_NIP7-like.
IPR016686. Ribosomal_synth_fac_NIP7.
[Graphical view]
PfamiPF03657. UPF0113. 1 hit.
[Graphical view]
PIRSFiPIRSF017190. Rbsml_synth_fac_NIP7. 1 hit.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQLTEEETK VVFEKLAGYI GRNISFLVDN KELPHVFRLQ KDRVYYVPDH
60 70 80 90 100
VAKLATSVAR PNLMSLGICL GKFTKTGKFR LHITSLTVLA KHAKYKIWIK
110 120 130 140 150
PNGEMPFLYG NHVLKAHVGK MSDDIPEHAG VIVFAMNDVP LGFGVSAKST
160 170 180
SESRNMQPTG IVAFRQADIG EYLRDEDTLF T
Length:181
Mass (Da):20,381
Last modified:November 1, 1996 - v1
Checksum:i82EAE8E4B2FCC31A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73567 Genomic DNA. Translation: CAA97926.1.
AY558059 Genomic DNA. Translation: AAS56385.1.
BK006949 Genomic DNA. Translation: DAA11225.1.
PIRiS65230.
RefSeqiNP_015113.1. NM_001184025.1.

Genome annotation databases

EnsemblFungiiYPL211W; YPL211W; YPL211W.
GeneIDi855890.
KEGGisce:YPL211W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73567 Genomic DNA. Translation: CAA97926.1.
AY558059 Genomic DNA. Translation: AAS56385.1.
BK006949 Genomic DNA. Translation: DAA11225.1.
PIRiS65230.
RefSeqiNP_015113.1. NM_001184025.1.

3D structure databases

ProteinModelPortaliQ08962.
SMRiQ08962. Positions 1-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35974. 108 interactions.
DIPiDIP-1456N.
IntActiQ08962. 46 interactions.
MINTiMINT-400883.

PTM databases

iPTMnetiQ08962.

Proteomic databases

MaxQBiQ08962.
PeptideAtlasiQ08962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL211W; YPL211W; YPL211W.
GeneIDi855890.
KEGGisce:YPL211W.

Organism-specific databases

EuPathDBiFungiDB:YPL211W.
SGDiS000006132. NIP7.

Phylogenomic databases

GeneTreeiENSGT00390000003591.
HOGENOMiHOG000168475.
InParanoidiQ08962.
KOiK07565.
OMAiVVKAHVG.
OrthoDBiEOG7K0ZQF.

Enzyme and pathway databases

BioCyciYEAST:G3O-34102-MONOMER.

Miscellaneous databases

NextBioi980562.
PROiQ08962.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR005155. Rbsml_synth_fac_NIP7-like.
IPR016686. Ribosomal_synth_fac_NIP7.
[Graphical view]
PfamiPF03657. UPF0113. 1 hit.
[Graphical view]
PIRSFiPIRSF017190. Rbsml_synth_fac_NIP7. 1 hit.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis."
    Zanchin N.I.T., Roberts P., DeSilva A., Sherman F., Goldfarb D.S.
    Mol. Cell. Biol. 17:5001-5015(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p."
    Zanchin N.I.T., Goldfarb D.S.
    Mol. Cell. Biol. 19:1518-1525(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, INTERACTION WITH NOP8 AND RRP43.
  6. "Identification of a 60S preribosomal particle that is closely linked to nuclear export."
    Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
    Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRE-RIBOSOME COMPLEX.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA."
    Coltri P.P., Guimaraes B.G., Granato D.C., Luz J.S., Teixeira E.C., Oliveira C.C., Zanchin N.I.
    Biochemistry 46:14177-14187(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING, MUTAGENESIS OF 161-ILE--PHE-164.

Entry informationi

Entry nameiNIP7_YEAST
AccessioniPrimary (citable) accession number: Q08962
Secondary accession number(s): D6W3F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.