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Q08957 (AFT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Iron-regulated transcriptional activator AFT2
Alternative name(s):
Activator of iron transcription protein 2
Gene names
Name:AFT2
Ordered Locus Names:YPL202C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor required for iron homeostasis and resistance to oxidative stress. With RCS1, activates the gene expression in response to low-iron conditions, also called iron regulon. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus Probable.

Miscellaneous

The transcription activation by RCS1 and AST2 depends on the mitochondrial iron-sulfur protein biosynthesis pathway.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Iron-regulated transcriptional activator AFT2
PRO_0000227599

Secondary structure

................... 416
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q08957 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0DAC56291EE56EFA

FASTA41647,105
        10         20         30         40         50         60 
MKAKSMKSII SVPISVSKTG KMKLTASPDN LASMMSKDQN KLIHLDPVPS FEDRHEIKPW 

        70         80         90        100        110        120 
LQKIFYPQGI DIVIERSDSS KVTFKCRSVR SKVGLNPKSK GSSSRSHACP FRIRAAYSVR 

       130        140        150        160        170        180 
LQKWNVVVMN NIHSHELRFD LITKTDDYKK FKENLRQKND EKAIKTFDEL EYKASLNLPL 

       190        200        210        220        230        240 
VTPIISCDCG LTKEIEAFNN IFLPLSNPPL TSKKNLLKTN KNSVSKIKSR QMDNSKPRPR 

       250        260        270        280        290        300 
LKTKLDADLH DTGFLDNFKT RNSCVKIEKE DSLTNLNEID FTNMFCNDNF IQNYNQGLME 

       310        320        330        340        350        360 
LLTEPTPGPS SSSCILPSTP TRPLSQSKMD IALSESTTSS PNFMETDAPY GDEIIKVSKD 

       370        380        390        400        410 
TKSNAPTADT DIATNLGKER NENFGMLNYN YEALLHFNDE HFNELNSIDP ALISKY 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast."
Blaiseau P.-L., Lesuisse E., Camadro J.-M.
J. Biol. Chem. 276:34221-34226(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"A second iron-regulatory system in yeast independent of Aft1p."
Rutherford J.C., Jaron S., Ray E., Brown P.O., Winge D.R.
Proc. Natl. Acad. Sci. U.S.A. 98:14322-14327(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements."
Rutherford J.C., Jaron S., Winge D.R.
J. Biol. Chem. 278:27636-27643(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis."
Rutherford J.C., Ojeda L., Balk J., Muehlenhoff U., Lill R., Winge D.R.
J. Biol. Chem. 280:10135-10140(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1."
Courel M., Lallet S., Camadro J.-M., Blaiseau P.-L.
Mol. Cell. Biol. 25:6760-6771(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73558 Genomic DNA. Translation: CAA97916.1.
BK006949 Genomic DNA. Translation: DAA11233.1.
PIRS65221.
RefSeqNP_015122.1. NM_001184016.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4LMGX-ray2.20A/B/C/D38-193[»]
ProteinModelPortalQ08957.
SMRQ08957. Positions 42-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35982. 25 interactions.
MINTMINT-596804.
STRING4932.YPL202C.

Proteomic databases

MaxQBQ08957.
PaxDbQ08957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL202C; YPL202C; YPL202C.
GeneID855899.
KEGGsce:YPL202C.

Organism-specific databases

CYGDYPL202c.
SGDS000006123. AFT2.

Phylogenomic databases

eggNOGNOG42685.
GeneTreeENSGT00530000069471.
OMADELEYKA.
OrthoDBEOG7XWPZ1.

Enzyme and pathway databases

BioCycYEAST:G3O-34094-MONOMER.

Gene expression databases

GenevestigatorQ08957.

Family and domain databases

InterProIPR014842. AFT.
[Graphical view]
PfamPF08731. AFT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio980586.

Entry information

Entry nameAFT2_YEAST
AccessionPrimary (citable) accession number: Q08957
Secondary accession number(s): D6W3G7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references