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Q08957

- AFT2_YEAST

UniProt

Q08957 - AFT2_YEAST

Protein

Iron-regulated transcriptional activator AFT2

Gene

AFT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcription factor required for iron homeostasis and resistance to oxidative stress. With RCS1, activates the gene expression in response to low-iron conditions, also called iron regulon.5 Publications

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD

    GO - Biological processi

    1. cellular iron ion homeostasis Source: SGD
    2. cellular response to oxidative stress Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34094-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iron-regulated transcriptional activator AFT2
    Alternative name(s):
    Activator of iron transcription protein 2
    Gene namesi
    Name:AFT2
    Ordered Locus Names:YPL202C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL202c.
    SGDiS000006123. AFT2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Iron-regulated transcriptional activator AFT2PRO_0000227599Add
    BLAST

    Proteomic databases

    MaxQBiQ08957.
    PaxDbiQ08957.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08957.

    Interactioni

    Protein-protein interaction databases

    BioGridi35982. 25 interactions.
    MINTiMINT-596804.
    STRINGi4932.YPL202C.

    Structurei

    Secondary structure

    1
    416
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi54 – 563
    Helixi57 – 659
    Helixi66 – 683
    Beta strandi72 – 787
    Beta strandi81 – 866
    Beta strandi112 – 1187
    Turni119 – 1224
    Beta strandi123 – 1297
    Helixi139 – 1424
    Helixi146 – 15712
    Helixi161 – 17515

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4LMGX-ray2.20A/B/C/D38-193[»]
    ProteinModelPortaliQ08957.
    SMRiQ08957. Positions 42-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG42685.
    GeneTreeiENSGT00530000069471.
    OMAiDELEYKA.
    OrthoDBiEOG7XWPZ1.

    Family and domain databases

    InterProiIPR014842. AFT.
    [Graphical view]
    PfamiPF08731. AFT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08957-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAKSMKSII SVPISVSKTG KMKLTASPDN LASMMSKDQN KLIHLDPVPS    50
    FEDRHEIKPW LQKIFYPQGI DIVIERSDSS KVTFKCRSVR SKVGLNPKSK 100
    GSSSRSHACP FRIRAAYSVR LQKWNVVVMN NIHSHELRFD LITKTDDYKK 150
    FKENLRQKND EKAIKTFDEL EYKASLNLPL VTPIISCDCG LTKEIEAFNN 200
    IFLPLSNPPL TSKKNLLKTN KNSVSKIKSR QMDNSKPRPR LKTKLDADLH 250
    DTGFLDNFKT RNSCVKIEKE DSLTNLNEID FTNMFCNDNF IQNYNQGLME 300
    LLTEPTPGPS SSSCILPSTP TRPLSQSKMD IALSESTTSS PNFMETDAPY 350
    GDEIIKVSKD TKSNAPTADT DIATNLGKER NENFGMLNYN YEALLHFNDE 400
    HFNELNSIDP ALISKY 416
    Length:416
    Mass (Da):47,105
    Last modified:November 1, 1996 - v1
    Checksum:i0DAC56291EE56EFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73558 Genomic DNA. Translation: CAA97916.1.
    BK006949 Genomic DNA. Translation: DAA11233.1.
    PIRiS65221.
    RefSeqiNP_015122.1. NM_001184016.1.

    Genome annotation databases

    EnsemblFungiiYPL202C; YPL202C; YPL202C.
    GeneIDi855899.
    KEGGisce:YPL202C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73558 Genomic DNA. Translation: CAA97916.1 .
    BK006949 Genomic DNA. Translation: DAA11233.1 .
    PIRi S65221.
    RefSeqi NP_015122.1. NM_001184016.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4LMG X-ray 2.20 A/B/C/D 38-193 [» ]
    ProteinModelPortali Q08957.
    SMRi Q08957. Positions 42-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35982. 25 interactions.
    MINTi MINT-596804.
    STRINGi 4932.YPL202C.

    Proteomic databases

    MaxQBi Q08957.
    PaxDbi Q08957.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL202C ; YPL202C ; YPL202C .
    GeneIDi 855899.
    KEGGi sce:YPL202C.

    Organism-specific databases

    CYGDi YPL202c.
    SGDi S000006123. AFT2.

    Phylogenomic databases

    eggNOGi NOG42685.
    GeneTreei ENSGT00530000069471.
    OMAi DELEYKA.
    OrthoDBi EOG7XWPZ1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34094-MONOMER.

    Miscellaneous databases

    NextBioi 980586.

    Gene expression databases

    Genevestigatori Q08957.

    Family and domain databases

    InterProi IPR014842. AFT.
    [Graphical view ]
    Pfami PF08731. AFT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast."
      Blaiseau P.-L., Lesuisse E., Camadro J.-M.
      J. Biol. Chem. 276:34221-34226(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: FUNCTION.
    5. "Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements."
      Rutherford J.C., Jaron S., Winge D.R.
      J. Biol. Chem. 278:27636-27643(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis."
      Rutherford J.C., Ojeda L., Balk J., Muehlenhoff U., Lill R., Winge D.R.
      J. Biol. Chem. 280:10135-10140(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1."
      Courel M., Lallet S., Camadro J.-M., Blaiseau P.-L.
      Mol. Cell. Biol. 25:6760-6771(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAFT2_YEAST
    AccessioniPrimary (citable) accession number: Q08957
    Secondary accession number(s): D6W3G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The transcription activation by RCS1 and AST2 depends on the mitochondrial iron-sulfur protein biosynthesis pathway.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3