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Q08957

- AFT2_YEAST

UniProt

Q08957 - AFT2_YEAST

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Protein

Iron-regulated transcriptional activator AFT2

Gene

AFT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor required for iron homeostasis and resistance to oxidative stress. With RCS1, activates the gene expression in response to low-iron conditions, also called iron regulon.5 Publications

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD

GO - Biological processi

  1. cellular iron ion homeostasis Source: SGD
  2. cellular response to oxidative stress Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciYEAST:G3O-34094-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-regulated transcriptional activator AFT2
Alternative name(s):
Activator of iron transcription protein 2
Gene namesi
Name:AFT2
Ordered Locus Names:YPL202C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL202c.
SGDiS000006123. AFT2.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Iron-regulated transcriptional activator AFT2PRO_0000227599Add
BLAST

Proteomic databases

MaxQBiQ08957.
PaxDbiQ08957.

Expressioni

Gene expression databases

GenevestigatoriQ08957.

Interactioni

Protein-protein interaction databases

BioGridi35982. 26 interactions.
MINTiMINT-596804.
STRINGi4932.YPL202C.

Structurei

Secondary structure

1
416
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi54 – 563Combined sources
Helixi57 – 659Combined sources
Helixi66 – 683Combined sources
Beta strandi72 – 787Combined sources
Beta strandi81 – 866Combined sources
Beta strandi112 – 1187Combined sources
Turni119 – 1224Combined sources
Beta strandi123 – 1297Combined sources
Helixi139 – 1424Combined sources
Helixi146 – 15712Combined sources
Helixi161 – 17515Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LMGX-ray2.20A/B/C/D38-193[»]
ProteinModelPortaliQ08957.
SMRiQ08957. Positions 42-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG42685.
GeneTreeiENSGT00530000069471.
InParanoidiQ08957.
OMAiDELEYKA.
OrthoDBiEOG7XWPZ1.

Family and domain databases

InterProiIPR014842. AFT.
[Graphical view]
PfamiPF08731. AFT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08957-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAKSMKSII SVPISVSKTG KMKLTASPDN LASMMSKDQN KLIHLDPVPS
60 70 80 90 100
FEDRHEIKPW LQKIFYPQGI DIVIERSDSS KVTFKCRSVR SKVGLNPKSK
110 120 130 140 150
GSSSRSHACP FRIRAAYSVR LQKWNVVVMN NIHSHELRFD LITKTDDYKK
160 170 180 190 200
FKENLRQKND EKAIKTFDEL EYKASLNLPL VTPIISCDCG LTKEIEAFNN
210 220 230 240 250
IFLPLSNPPL TSKKNLLKTN KNSVSKIKSR QMDNSKPRPR LKTKLDADLH
260 270 280 290 300
DTGFLDNFKT RNSCVKIEKE DSLTNLNEID FTNMFCNDNF IQNYNQGLME
310 320 330 340 350
LLTEPTPGPS SSSCILPSTP TRPLSQSKMD IALSESTTSS PNFMETDAPY
360 370 380 390 400
GDEIIKVSKD TKSNAPTADT DIATNLGKER NENFGMLNYN YEALLHFNDE
410
HFNELNSIDP ALISKY
Length:416
Mass (Da):47,105
Last modified:November 1, 1996 - v1
Checksum:i0DAC56291EE56EFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73558 Genomic DNA. Translation: CAA97916.1.
BK006949 Genomic DNA. Translation: DAA11233.1.
PIRiS65221.
RefSeqiNP_015122.1. NM_001184016.1.

Genome annotation databases

EnsemblFungiiYPL202C; YPL202C; YPL202C.
GeneIDi855899.
KEGGisce:YPL202C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73558 Genomic DNA. Translation: CAA97916.1 .
BK006949 Genomic DNA. Translation: DAA11233.1 .
PIRi S65221.
RefSeqi NP_015122.1. NM_001184016.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4LMG X-ray 2.20 A/B/C/D 38-193 [» ]
ProteinModelPortali Q08957.
SMRi Q08957. Positions 42-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35982. 26 interactions.
MINTi MINT-596804.
STRINGi 4932.YPL202C.

Proteomic databases

MaxQBi Q08957.
PaxDbi Q08957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL202C ; YPL202C ; YPL202C .
GeneIDi 855899.
KEGGi sce:YPL202C.

Organism-specific databases

CYGDi YPL202c.
SGDi S000006123. AFT2.

Phylogenomic databases

eggNOGi NOG42685.
GeneTreei ENSGT00530000069471.
InParanoidi Q08957.
OMAi DELEYKA.
OrthoDBi EOG7XWPZ1.

Enzyme and pathway databases

BioCyci YEAST:G3O-34094-MONOMER.

Miscellaneous databases

NextBioi 980586.

Gene expression databases

Genevestigatori Q08957.

Family and domain databases

InterProi IPR014842. AFT.
[Graphical view ]
Pfami PF08731. AFT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast."
    Blaiseau P.-L., Lesuisse E., Camadro J.-M.
    J. Biol. Chem. 276:34221-34226(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: FUNCTION.
  5. "Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements."
    Rutherford J.C., Jaron S., Winge D.R.
    J. Biol. Chem. 278:27636-27643(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis."
    Rutherford J.C., Ojeda L., Balk J., Muehlenhoff U., Lill R., Winge D.R.
    J. Biol. Chem. 280:10135-10140(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1."
    Courel M., Lallet S., Camadro J.-M., Blaiseau P.-L.
    Mol. Cell. Biol. 25:6760-6771(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAFT2_YEAST
AccessioniPrimary (citable) accession number: Q08957
Secondary accession number(s): D6W3G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The transcription activation by RCS1 and AST2 depends on the mitochondrial iron-sulfur protein biosynthesis pathway.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3