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Q08951 (AP3D_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-3 complex subunit delta
Alternative name(s):
Adapter-related protein complex 3 subunit delta
Delta-adaptin 3
Short name=Delta-adaptin
Gene names
Name:APL5
Synonyms:YKS4
Ordered Locus Names:YPL195W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length932 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole. Ref.4 Ref.5 Ref.6

Subunit structure

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3). Interacts with VPS41. Ref.6 Ref.7

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. Ref.6 Ref.8

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Contains 12 HEAT repeats.

Sequence caution

The sequence AAA79850.1 differs from that shown. Reason: Frameshift at positions 14 and 133.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

APL6P466825EBI-29702,EBI-2213
APM3P381533EBI-29702,EBI-2710

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 932931AP-3 complex subunit delta
PRO_0000227676

Regions

Repeat157 – 19438HEAT 1
Repeat196 – 23136HEAT 2
Repeat233 – 26937HEAT 3
Repeat270 – 30738HEAT 4
Repeat310 – 34637HEAT 5
Repeat347 – 38438HEAT 6
Repeat386 – 42540HEAT 7
Repeat427 – 46640HEAT 8
Repeat490 – 52738HEAT 9
Repeat528 – 56437HEAT 10
Repeat570 – 60132HEAT 11
Repeat602 – 63837HEAT 12
Coiled coil858 – 87821 Potential
Compositional bias784 – 7918Poly-Lys
Compositional bias897 – 91620Poly-Lys

Amino acid modifications

Modified residue21N-acetylthreonine Ref.13
Modified residue7001Phosphoserine Ref.12
Modified residue7271Phosphoserine Ref.11
Modified residue7671Phosphothreonine Ref.10 Ref.12
Modified residue7701Phosphoserine Ref.12
Modified residue7731Phosphoserine Ref.12
Modified residue7981Phosphoserine Ref.11 Ref.12
Modified residue8881Phosphoserine Ref.10 Ref.12
Modified residue9181Phosphoserine Ref.10 Ref.11 Ref.12

Sequences

Sequence LengthMass (Da)Tools
Q08951 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 51415466FF25CC40

FASTA932106,924
        10         20         30         40         50         60 
MTSLYAPGAE DIRQRLRPFG FFFEKSLKDL IKGIRSHNET PEKLDQFFKQ VLSECREEVN 

        70         80         90        100        110        120 
SPDLNSKTNA VLKLTYLEMY GFDMAWCNFH ILEVMSSNKL QQKRVGYLAA SQSFYKDSDI 

       130        140        150        160        170        180 
LMLATNLLKK DLKYDGNNDV VKVGIALSGL STIITPSLAR DIADDLFTML NSTRPYIRKK 

       190        200        210        220        230        240 
AITALFKVFL QYPEALRDNF DKFVSKLDDD DISVVSAAVS VICELSKKNP QPFIQLSPLL 

       250        260        270        280        290        300 
YEILVTIDNN WIIIRLLKLF TNLSQVEPKL RAKLLPKILE LMESTVATSV IYESVNCIVK 

       310        320        330        340        350        360 
GNMLEEDDFE TAMACLERLH TFCDSQDPNL RYISCILFYK IGKINTDFIS RFDQLIIRLL 

       370        380        390        400        410        420 
SDVDVSIRSK AIELVEGIVD EDNLKAIVQT LMKQFVDEDV VILQTGSIVY EKSKRIPIII 

       430        440        450        460        470        480 
PENYKIKMVN VIISICSADN YSSVNDFEWY NAVIMDLAML CQDISDKSLG SKIGEQFRNL 

       490        500        510        520        530        540 
MIKVPSMREV TIANIIKLIS NDNINKQLPT VLRECIWCLG EFSTLVENGN DLIKIMTENI 

       550        560        570        580        590        600 
SYYSHSVQEV LILALVKVFS NWCNNFQEDK RFEIKMVLKE LIEFFENLSY SSTFEVQERS 

       610        620        630        640        650        660 
VEVLEFLRLS LEALEEDTEG LPMLLSEVLP SFFNAYELAP IARGTQLKLA VDENLDLETP 

       670        680        690        700        710        720 
FLTKEAADEL LDEQKSDAIS DLMSDISMDE QVELKFVDDS DTSYEEKEKL DDFENPFEIE 

       730        740        750        760        770        780 
REKERMSNPY YLGEEDEERT KNSKDLLDLN EEESSDKKPE TIRLNRTDNS LNSLSLSTTE 

       790        800        810        820        830        840 
ISRKKKKGKK KNRVQVLSDE PVIEAAPKRK DAFQKPHDNH STQNPLKKDK INLRMHSQLE 

       850        860        870        880        890        900 
NFDFSNFGQS SNAGRGSQEE GNLRKEDELE LSRLEANLIV KDEKDNLSDT EEVIVIKKKK 

       910        920        930 
KGKKSKSKNK LKTKAKNSPE PNEFLRDQST DI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Suppressors of loss of yeast casein kinase 1 function define the four subunits of a novel putative adaptin complex."
Robinson L.C., Engle H.M., Panek H.R.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-932.
[4]"The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
[5]"Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
[6]"Formation of AP-3 transport intermediates requires Vps41 function."
Rehling P., Darsow T., Katzmann D.J., Emr S.D.
Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH VPS41, FUNCTION OF THE AP-3 COMPLEX.
[7]"Vps41p function in the alkaline phosphatase pathway requires homo-oligomerization and interaction with AP-3 through two distinct domains."
Darsow T., Katzmann D.J., Cowles C.R., Emr S.D.
Mol. Biol. Cell 12:37-51(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VPS41.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-767; SER-888 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-727; SER-798 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-700; THR-767; SER-770; SER-773; SER-798; SER-888 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73551 Genomic DNA. Translation: CAA97908.1.
U36858 Genomic DNA. Translation: AAA79850.1. Frameshift.
BK006949 Genomic DNA. Translation: DAA11239.1.
PIRS65214.
RefSeqNP_015129.1. NM_001184009.1.

3D structure databases

ProteinModelPortalQ08951.
SMRQ08951. Positions 27-568.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35988. 109 interactions.
DIPDIP-3977N.
IntActQ08951. 6 interactions.
MINTMINT-565984.

Proteomic databases

PaxDbQ08951.
PeptideAtlasQ08951.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL195W; YPL195W; YPL195W.
GeneID855906.
KEGGsce:YPL195W.

Organism-specific databases

CYGDYPL195w.
SGDS000006116. APL5.

Phylogenomic databases

eggNOGNOG285451.
GeneTreeENSGT00550000075067.
HOGENOMHOG000000981.
OMAQQKRVGY.
OrthoDBEOG7ZD23T.

Enzyme and pathway databases

BioCycYEAST:G3O-34088-MONOMER.

Gene expression databases

GenevestigatorQ08951.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR017105. AP3_complex_dsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamPF01602. Adaptin_N. 1 hit.
[Graphical view]
PIRSFPIRSF037092. AP3_complex_delta. 1 hit.
SUPFAMSSF48371. SSF48371. 2 hits.
ProtoNetSearch...

Other

NextBio980604.

Entry information

Entry nameAP3D_YEAST
AccessionPrimary (citable) accession number: Q08951
Secondary accession number(s): D6W3H3, Q02737, Q7LIB1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families