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Protein

AP-3 complex subunit delta

Gene

APL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

GO - Molecular functioni

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34088-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit delta
Alternative name(s):
Adaptor-related protein complex 3 subunit delta
Delta-adaptin 3
Short name:
Delta-adaptin
Gene namesi
Name:APL5
Synonyms:YKS4
Ordered Locus Names:YPL195W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL195W.
SGDiS000006116. APL5.

Subcellular locationi

GO - Cellular componenti

  • AP-3 adaptor complex Source: SGD
  • clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002276762 – 932AP-3 complex subunit deltaAdd BLAST931

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei700PhosphoserineCombined sources1
Modified residuei727PhosphoserineCombined sources1
Modified residuei767PhosphothreonineCombined sources1
Modified residuei770PhosphoserineCombined sources1
Modified residuei773PhosphoserineCombined sources1
Modified residuei798PhosphoserineCombined sources1
Modified residuei888PhosphoserineCombined sources1
Modified residuei918PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08951.
PRIDEiQ08951.

PTM databases

iPTMnetiQ08951.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3). Interacts with VPS41.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APL6P466825EBI-29702,EBI-2213
APM3P381533EBI-29702,EBI-2710

Protein-protein interaction databases

BioGridi35988. 110 interactors.
DIPiDIP-3977N.
IntActiQ08951. 7 interactors.
MINTiMINT-565984.

Structurei

3D structure databases

ProteinModelPortaliQ08951.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati157 – 194HEAT 1Add BLAST38
Repeati196 – 231HEAT 2Add BLAST36
Repeati233 – 269HEAT 3Add BLAST37
Repeati270 – 307HEAT 4Add BLAST38
Repeati310 – 346HEAT 5Add BLAST37
Repeati347 – 384HEAT 6Add BLAST38
Repeati386 – 425HEAT 7Add BLAST40
Repeati427 – 466HEAT 8Add BLAST40
Repeati490 – 527HEAT 9Add BLAST38
Repeati528 – 564HEAT 10Add BLAST37
Repeati570 – 601HEAT 11Add BLAST32
Repeati602 – 638HEAT 12Add BLAST37

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili858 – 878Sequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi784 – 791Poly-Lys8
Compositional biasi897 – 916Poly-LysAdd BLAST20

Sequence similaritiesi

Contains 12 HEAT repeats.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075067.
HOGENOMiHOG000000981.
InParanoidiQ08951.
OMAiYESINCI.
OrthoDBiEOG092C0TL9.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR017105. AP3_complex_dsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037092. AP3_complex_delta. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLYAPGAE DIRQRLRPFG FFFEKSLKDL IKGIRSHNET PEKLDQFFKQ
60 70 80 90 100
VLSECREEVN SPDLNSKTNA VLKLTYLEMY GFDMAWCNFH ILEVMSSNKL
110 120 130 140 150
QQKRVGYLAA SQSFYKDSDI LMLATNLLKK DLKYDGNNDV VKVGIALSGL
160 170 180 190 200
STIITPSLAR DIADDLFTML NSTRPYIRKK AITALFKVFL QYPEALRDNF
210 220 230 240 250
DKFVSKLDDD DISVVSAAVS VICELSKKNP QPFIQLSPLL YEILVTIDNN
260 270 280 290 300
WIIIRLLKLF TNLSQVEPKL RAKLLPKILE LMESTVATSV IYESVNCIVK
310 320 330 340 350
GNMLEEDDFE TAMACLERLH TFCDSQDPNL RYISCILFYK IGKINTDFIS
360 370 380 390 400
RFDQLIIRLL SDVDVSIRSK AIELVEGIVD EDNLKAIVQT LMKQFVDEDV
410 420 430 440 450
VILQTGSIVY EKSKRIPIII PENYKIKMVN VIISICSADN YSSVNDFEWY
460 470 480 490 500
NAVIMDLAML CQDISDKSLG SKIGEQFRNL MIKVPSMREV TIANIIKLIS
510 520 530 540 550
NDNINKQLPT VLRECIWCLG EFSTLVENGN DLIKIMTENI SYYSHSVQEV
560 570 580 590 600
LILALVKVFS NWCNNFQEDK RFEIKMVLKE LIEFFENLSY SSTFEVQERS
610 620 630 640 650
VEVLEFLRLS LEALEEDTEG LPMLLSEVLP SFFNAYELAP IARGTQLKLA
660 670 680 690 700
VDENLDLETP FLTKEAADEL LDEQKSDAIS DLMSDISMDE QVELKFVDDS
710 720 730 740 750
DTSYEEKEKL DDFENPFEIE REKERMSNPY YLGEEDEERT KNSKDLLDLN
760 770 780 790 800
EEESSDKKPE TIRLNRTDNS LNSLSLSTTE ISRKKKKGKK KNRVQVLSDE
810 820 830 840 850
PVIEAAPKRK DAFQKPHDNH STQNPLKKDK INLRMHSQLE NFDFSNFGQS
860 870 880 890 900
SNAGRGSQEE GNLRKEDELE LSRLEANLIV KDEKDNLSDT EEVIVIKKKK
910 920 930
KGKKSKSKNK LKTKAKNSPE PNEFLRDQST DI
Length:932
Mass (Da):106,924
Last modified:November 1, 1996 - v1
Checksum:i51415466FF25CC40
GO

Sequence cautioni

The sequence AAA79850 differs from that shown. Reason: Frameshift at positions 14 and 133.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73551 Genomic DNA. Translation: CAA97908.1.
U36858 Genomic DNA. Translation: AAA79850.1. Frameshift.
BK006949 Genomic DNA. Translation: DAA11239.1.
PIRiS65214.
RefSeqiNP_015129.1. NM_001184009.1.

Genome annotation databases

EnsemblFungiiYPL195W; YPL195W; YPL195W.
GeneIDi855906.
KEGGisce:YPL195W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73551 Genomic DNA. Translation: CAA97908.1.
U36858 Genomic DNA. Translation: AAA79850.1. Frameshift.
BK006949 Genomic DNA. Translation: DAA11239.1.
PIRiS65214.
RefSeqiNP_015129.1. NM_001184009.1.

3D structure databases

ProteinModelPortaliQ08951.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35988. 110 interactors.
DIPiDIP-3977N.
IntActiQ08951. 7 interactors.
MINTiMINT-565984.

PTM databases

iPTMnetiQ08951.

Proteomic databases

MaxQBiQ08951.
PRIDEiQ08951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL195W; YPL195W; YPL195W.
GeneIDi855906.
KEGGisce:YPL195W.

Organism-specific databases

EuPathDBiFungiDB:YPL195W.
SGDiS000006116. APL5.

Phylogenomic databases

GeneTreeiENSGT00550000075067.
HOGENOMiHOG000000981.
InParanoidiQ08951.
OMAiYESINCI.
OrthoDBiEOG092C0TL9.

Enzyme and pathway databases

BioCyciYEAST:G3O-34088-MONOMER.

Miscellaneous databases

PROiQ08951.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR017105. AP3_complex_dsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037092. AP3_complex_delta. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiAP3D_YEAST
AccessioniPrimary (citable) accession number: Q08951
Secondary accession number(s): D6W3H3, Q02737, Q7LIB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.