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Q08951

- AP3D_YEAST

UniProt

Q08951 - AP3D_YEAST

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Protein

AP-3 complex subunit delta

Gene

APL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

GO - Molecular functioni

  1. protein transporter activity Source: InterPro

GO - Biological processi

  1. Golgi to vacuole transport Source: SGD
  2. protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34088-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit delta
Alternative name(s):
Adaptor-related protein complex 3 subunit delta
Delta-adaptin 3
Short name:
Delta-adaptin
Gene namesi
Name:APL5
Synonyms:YKS4
Ordered Locus Names:YPL195W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL195w.
SGDiS000006116. APL5.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. AP-3 adaptor complex Source: SGD
  2. cytoplasmic vesicle Source: UniProtKB-KW
  3. Golgi apparatus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 932931AP-3 complex subunit deltaPRO_0000227676Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei700 – 7001Phosphoserine1 Publication
Modified residuei727 – 7271Phosphoserine1 Publication
Modified residuei767 – 7671Phosphothreonine2 Publications
Modified residuei770 – 7701Phosphoserine1 Publication
Modified residuei773 – 7731Phosphoserine1 Publication
Modified residuei798 – 7981Phosphoserine2 Publications
Modified residuei888 – 8881Phosphoserine2 Publications
Modified residuei918 – 9181Phosphoserine3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08951.
PaxDbiQ08951.
PeptideAtlasiQ08951.

Expressioni

Gene expression databases

GenevestigatoriQ08951.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3). Interacts with VPS41.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APL6P466825EBI-29702,EBI-2213
APM3P381533EBI-29702,EBI-2710

Protein-protein interaction databases

BioGridi35988. 109 interactions.
DIPiDIP-3977N.
IntActiQ08951. 6 interactions.
MINTiMINT-565984.

Structurei

3D structure databases

ProteinModelPortaliQ08951.
SMRiQ08951. Positions 27-392, 582-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati157 – 19438HEAT 1Add
BLAST
Repeati196 – 23136HEAT 2Add
BLAST
Repeati233 – 26937HEAT 3Add
BLAST
Repeati270 – 30738HEAT 4Add
BLAST
Repeati310 – 34637HEAT 5Add
BLAST
Repeati347 – 38438HEAT 6Add
BLAST
Repeati386 – 42540HEAT 7Add
BLAST
Repeati427 – 46640HEAT 8Add
BLAST
Repeati490 – 52738HEAT 9Add
BLAST
Repeati528 – 56437HEAT 10Add
BLAST
Repeati570 – 60132HEAT 11Add
BLAST
Repeati602 – 63837HEAT 12Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili858 – 87821Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi784 – 7918Poly-Lys
Compositional biasi897 – 91620Poly-LysAdd
BLAST

Sequence similaritiesi

Contains 12 HEAT repeats.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG285451.
GeneTreeiENSGT00550000075067.
HOGENOMiHOG000000981.
InParanoidiQ08951.
OMAiQQKRVGY.
OrthoDBiEOG7ZD23T.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR017105. AP3_complex_dsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037092. AP3_complex_delta. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08951-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSLYAPGAE DIRQRLRPFG FFFEKSLKDL IKGIRSHNET PEKLDQFFKQ
60 70 80 90 100
VLSECREEVN SPDLNSKTNA VLKLTYLEMY GFDMAWCNFH ILEVMSSNKL
110 120 130 140 150
QQKRVGYLAA SQSFYKDSDI LMLATNLLKK DLKYDGNNDV VKVGIALSGL
160 170 180 190 200
STIITPSLAR DIADDLFTML NSTRPYIRKK AITALFKVFL QYPEALRDNF
210 220 230 240 250
DKFVSKLDDD DISVVSAAVS VICELSKKNP QPFIQLSPLL YEILVTIDNN
260 270 280 290 300
WIIIRLLKLF TNLSQVEPKL RAKLLPKILE LMESTVATSV IYESVNCIVK
310 320 330 340 350
GNMLEEDDFE TAMACLERLH TFCDSQDPNL RYISCILFYK IGKINTDFIS
360 370 380 390 400
RFDQLIIRLL SDVDVSIRSK AIELVEGIVD EDNLKAIVQT LMKQFVDEDV
410 420 430 440 450
VILQTGSIVY EKSKRIPIII PENYKIKMVN VIISICSADN YSSVNDFEWY
460 470 480 490 500
NAVIMDLAML CQDISDKSLG SKIGEQFRNL MIKVPSMREV TIANIIKLIS
510 520 530 540 550
NDNINKQLPT VLRECIWCLG EFSTLVENGN DLIKIMTENI SYYSHSVQEV
560 570 580 590 600
LILALVKVFS NWCNNFQEDK RFEIKMVLKE LIEFFENLSY SSTFEVQERS
610 620 630 640 650
VEVLEFLRLS LEALEEDTEG LPMLLSEVLP SFFNAYELAP IARGTQLKLA
660 670 680 690 700
VDENLDLETP FLTKEAADEL LDEQKSDAIS DLMSDISMDE QVELKFVDDS
710 720 730 740 750
DTSYEEKEKL DDFENPFEIE REKERMSNPY YLGEEDEERT KNSKDLLDLN
760 770 780 790 800
EEESSDKKPE TIRLNRTDNS LNSLSLSTTE ISRKKKKGKK KNRVQVLSDE
810 820 830 840 850
PVIEAAPKRK DAFQKPHDNH STQNPLKKDK INLRMHSQLE NFDFSNFGQS
860 870 880 890 900
SNAGRGSQEE GNLRKEDELE LSRLEANLIV KDEKDNLSDT EEVIVIKKKK
910 920 930
KGKKSKSKNK LKTKAKNSPE PNEFLRDQST DI
Length:932
Mass (Da):106,924
Last modified:November 1, 1996 - v1
Checksum:i51415466FF25CC40
GO

Sequence cautioni

The sequence AAA79850.1 differs from that shown. Reason: Frameshift at positions 14 and 133. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73551 Genomic DNA. Translation: CAA97908.1.
U36858 Genomic DNA. Translation: AAA79850.1. Frameshift.
BK006949 Genomic DNA. Translation: DAA11239.1.
PIRiS65214.
RefSeqiNP_015129.1. NM_001184009.1.

Genome annotation databases

EnsemblFungiiYPL195W; YPL195W; YPL195W.
GeneIDi855906.
KEGGisce:YPL195W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73551 Genomic DNA. Translation: CAA97908.1 .
U36858 Genomic DNA. Translation: AAA79850.1 . Frameshift.
BK006949 Genomic DNA. Translation: DAA11239.1 .
PIRi S65214.
RefSeqi NP_015129.1. NM_001184009.1.

3D structure databases

ProteinModelPortali Q08951.
SMRi Q08951. Positions 27-392, 582-607.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35988. 109 interactions.
DIPi DIP-3977N.
IntActi Q08951. 6 interactions.
MINTi MINT-565984.

Proteomic databases

MaxQBi Q08951.
PaxDbi Q08951.
PeptideAtlasi Q08951.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL195W ; YPL195W ; YPL195W .
GeneIDi 855906.
KEGGi sce:YPL195W.

Organism-specific databases

CYGDi YPL195w.
SGDi S000006116. APL5.

Phylogenomic databases

eggNOGi NOG285451.
GeneTreei ENSGT00550000075067.
HOGENOMi HOG000000981.
InParanoidi Q08951.
OMAi QQKRVGY.
OrthoDBi EOG7ZD23T.

Enzyme and pathway databases

BioCyci YEAST:G3O-34088-MONOMER.

Miscellaneous databases

NextBioi 980604.

Gene expression databases

Genevestigatori Q08951.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR017105. AP3_complex_dsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view ]
Pfami PF01602. Adaptin_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF037092. AP3_complex_delta. 1 hit.
SUPFAMi SSF48371. SSF48371. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Suppressors of loss of yeast casein kinase 1 function define the four subunits of a novel putative adaptin complex."
    Robinson L.C., Engle H.M., Panek H.R.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-932.
  4. "The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
    Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
    Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  5. "Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
    Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
    EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  6. "Formation of AP-3 transport intermediates requires Vps41 function."
    Rehling P., Darsow T., Katzmann D.J., Emr S.D.
    Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH VPS41, FUNCTION OF THE AP-3 COMPLEX.
  7. "Vps41p function in the alkaline phosphatase pathway requires homo-oligomerization and interaction with AP-3 through two distinct domains."
    Darsow T., Katzmann D.J., Cowles C.R., Emr S.D.
    Mol. Biol. Cell 12:37-51(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS41.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-767; SER-888 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-727; SER-798 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-700; THR-767; SER-770; SER-773; SER-798; SER-888 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP3D_YEAST
AccessioniPrimary (citable) accession number: Q08951
Secondary accession number(s): D6W3H3, Q02737, Q7LIB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3