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Q08951

- AP3D_YEAST

UniProt

Q08951 - AP3D_YEAST

Protein

AP-3 complex subunit delta

Gene

APL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein transporter activity Source: InterPro

    GO - Biological processi

    1. Golgi to vacuole transport Source: SGD
    2. protein targeting to vacuole Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34088-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-3 complex subunit delta
    Alternative name(s):
    Adaptor-related protein complex 3 subunit delta
    Delta-adaptin 3
    Short name:
    Delta-adaptin
    Gene namesi
    Name:APL5
    Synonyms:YKS4
    Ordered Locus Names:YPL195W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL195w.
    SGDiS000006116. APL5.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. AP-3 adaptor complex Source: SGD
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 932931AP-3 complex subunit deltaPRO_0000227676Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonine1 Publication
    Modified residuei700 – 7001Phosphoserine1 Publication
    Modified residuei727 – 7271Phosphoserine1 Publication
    Modified residuei767 – 7671Phosphothreonine2 Publications
    Modified residuei770 – 7701Phosphoserine1 Publication
    Modified residuei773 – 7731Phosphoserine1 Publication
    Modified residuei798 – 7981Phosphoserine2 Publications
    Modified residuei888 – 8881Phosphoserine2 Publications
    Modified residuei918 – 9181Phosphoserine3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ08951.
    PaxDbiQ08951.
    PeptideAtlasiQ08951.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08951.

    Interactioni

    Subunit structurei

    Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3). Interacts with VPS41.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APL6P466825EBI-29702,EBI-2213
    APM3P381533EBI-29702,EBI-2710

    Protein-protein interaction databases

    BioGridi35988. 109 interactions.
    DIPiDIP-3977N.
    IntActiQ08951. 6 interactions.
    MINTiMINT-565984.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08951.
    SMRiQ08951. Positions 27-568.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati157 – 19438HEAT 1Add
    BLAST
    Repeati196 – 23136HEAT 2Add
    BLAST
    Repeati233 – 26937HEAT 3Add
    BLAST
    Repeati270 – 30738HEAT 4Add
    BLAST
    Repeati310 – 34637HEAT 5Add
    BLAST
    Repeati347 – 38438HEAT 6Add
    BLAST
    Repeati386 – 42540HEAT 7Add
    BLAST
    Repeati427 – 46640HEAT 8Add
    BLAST
    Repeati490 – 52738HEAT 9Add
    BLAST
    Repeati528 – 56437HEAT 10Add
    BLAST
    Repeati570 – 60132HEAT 11Add
    BLAST
    Repeati602 – 63837HEAT 12Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili858 – 87821Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi784 – 7918Poly-Lys
    Compositional biasi897 – 91620Poly-LysAdd
    BLAST

    Sequence similaritiesi

    Contains 12 HEAT repeats.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG285451.
    GeneTreeiENSGT00550000075067.
    HOGENOMiHOG000000981.
    OMAiQQKRVGY.
    OrthoDBiEOG7ZD23T.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR017105. AP3_complex_dsu.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    [Graphical view]
    PfamiPF01602. Adaptin_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037092. AP3_complex_delta. 1 hit.
    SUPFAMiSSF48371. SSF48371. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08951-1 [UniParc]FASTAAdd to Basket

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    MTSLYAPGAE DIRQRLRPFG FFFEKSLKDL IKGIRSHNET PEKLDQFFKQ    50
    VLSECREEVN SPDLNSKTNA VLKLTYLEMY GFDMAWCNFH ILEVMSSNKL 100
    QQKRVGYLAA SQSFYKDSDI LMLATNLLKK DLKYDGNNDV VKVGIALSGL 150
    STIITPSLAR DIADDLFTML NSTRPYIRKK AITALFKVFL QYPEALRDNF 200
    DKFVSKLDDD DISVVSAAVS VICELSKKNP QPFIQLSPLL YEILVTIDNN 250
    WIIIRLLKLF TNLSQVEPKL RAKLLPKILE LMESTVATSV IYESVNCIVK 300
    GNMLEEDDFE TAMACLERLH TFCDSQDPNL RYISCILFYK IGKINTDFIS 350
    RFDQLIIRLL SDVDVSIRSK AIELVEGIVD EDNLKAIVQT LMKQFVDEDV 400
    VILQTGSIVY EKSKRIPIII PENYKIKMVN VIISICSADN YSSVNDFEWY 450
    NAVIMDLAML CQDISDKSLG SKIGEQFRNL MIKVPSMREV TIANIIKLIS 500
    NDNINKQLPT VLRECIWCLG EFSTLVENGN DLIKIMTENI SYYSHSVQEV 550
    LILALVKVFS NWCNNFQEDK RFEIKMVLKE LIEFFENLSY SSTFEVQERS 600
    VEVLEFLRLS LEALEEDTEG LPMLLSEVLP SFFNAYELAP IARGTQLKLA 650
    VDENLDLETP FLTKEAADEL LDEQKSDAIS DLMSDISMDE QVELKFVDDS 700
    DTSYEEKEKL DDFENPFEIE REKERMSNPY YLGEEDEERT KNSKDLLDLN 750
    EEESSDKKPE TIRLNRTDNS LNSLSLSTTE ISRKKKKGKK KNRVQVLSDE 800
    PVIEAAPKRK DAFQKPHDNH STQNPLKKDK INLRMHSQLE NFDFSNFGQS 850
    SNAGRGSQEE GNLRKEDELE LSRLEANLIV KDEKDNLSDT EEVIVIKKKK 900
    KGKKSKSKNK LKTKAKNSPE PNEFLRDQST DI 932
    Length:932
    Mass (Da):106,924
    Last modified:November 1, 1996 - v1
    Checksum:i51415466FF25CC40
    GO

    Sequence cautioni

    The sequence AAA79850.1 differs from that shown. Reason: Frameshift at positions 14 and 133.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73551 Genomic DNA. Translation: CAA97908.1.
    U36858 Genomic DNA. Translation: AAA79850.1. Frameshift.
    BK006949 Genomic DNA. Translation: DAA11239.1.
    PIRiS65214.
    RefSeqiNP_015129.1. NM_001184009.1.

    Genome annotation databases

    EnsemblFungiiYPL195W; YPL195W; YPL195W.
    GeneIDi855906.
    KEGGisce:YPL195W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73551 Genomic DNA. Translation: CAA97908.1 .
    U36858 Genomic DNA. Translation: AAA79850.1 . Frameshift.
    BK006949 Genomic DNA. Translation: DAA11239.1 .
    PIRi S65214.
    RefSeqi NP_015129.1. NM_001184009.1.

    3D structure databases

    ProteinModelPortali Q08951.
    SMRi Q08951. Positions 27-568.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35988. 109 interactions.
    DIPi DIP-3977N.
    IntActi Q08951. 6 interactions.
    MINTi MINT-565984.

    Proteomic databases

    MaxQBi Q08951.
    PaxDbi Q08951.
    PeptideAtlasi Q08951.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL195W ; YPL195W ; YPL195W .
    GeneIDi 855906.
    KEGGi sce:YPL195W.

    Organism-specific databases

    CYGDi YPL195w.
    SGDi S000006116. APL5.

    Phylogenomic databases

    eggNOGi NOG285451.
    GeneTreei ENSGT00550000075067.
    HOGENOMi HOG000000981.
    OMAi QQKRVGY.
    OrthoDBi EOG7ZD23T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34088-MONOMER.

    Miscellaneous databases

    NextBioi 980604.

    Gene expression databases

    Genevestigatori Q08951.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR017105. AP3_complex_dsu.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    [Graphical view ]
    Pfami PF01602. Adaptin_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037092. AP3_complex_delta. 1 hit.
    SUPFAMi SSF48371. SSF48371. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Suppressors of loss of yeast casein kinase 1 function define the four subunits of a novel putative adaptin complex."
      Robinson L.C., Engle H.M., Panek H.R.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-932.
    4. "The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
      Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
      Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
    5. "Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
      Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
      EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
    6. "Formation of AP-3 transport intermediates requires Vps41 function."
      Rehling P., Darsow T., Katzmann D.J., Emr S.D.
      Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH VPS41, FUNCTION OF THE AP-3 COMPLEX.
    7. "Vps41p function in the alkaline phosphatase pathway requires homo-oligomerization and interaction with AP-3 through two distinct domains."
      Darsow T., Katzmann D.J., Cowles C.R., Emr S.D.
      Mol. Biol. Cell 12:37-51(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VPS41.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-767; SER-888 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-727; SER-798 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-700; THR-767; SER-770; SER-773; SER-798; SER-888 AND SER-918, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP3D_YEAST
    AccessioniPrimary (citable) accession number: Q08951
    Secondary accession number(s): D6W3H3, Q02737, Q7LIB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3