Reviewed, UniProtKB/Swiss-Prot Q08949 (DDC1_YEAST)

Names and origin

Protein names

Recommended name:
    DNA damage checkpoint protein 1

Gene names

Name:	DDC1
Ordered Locus Names:	YPL194W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Associates with sites of DNA damage and modulates the MEC1 signaling pathway and the activation of RAD53 in response to DNA damage at phase G1. The complex also physically regulates DNA polymerase zeta-dependent mutagenesis by controlling the access of polymerase zeta to damaged DNA. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 Ref.13 Ref.15 Ref.16
Subunit structure	Component of the checkpoint clamp complex composed of DDC1, MEC3 and RAD17. The interaction with MEC3 is performed in a RAD17-dependent manner. The checkpoint clamp complex loads onto DNA in an ATP-dependent manner through its interaction with the RFC-RAD4 checkpoint clamp loader complex. Interacts with the DNA polymerase zeta subunit REV7 and DPB11. Ref.3 Ref.13 Ref.16 Ref.7 Ref.14 Ref.17
Subcellular location	Cytoplasm. Nucleus.
Post-translational modification	Phosphorylated during cell cycle S-phase and in response to DNA damage. This phosphorylation is MEC14 dependent. Also hosphorylated by CDC28. Ref.2 Ref.3 Ref.6 Ref.12 Ref.18 Ref.19
Miscellaneous	Present with 238 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the DDC1 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G1/S transition checkpoint Ref.2 Inferred from mutant phenotype. Source: SGD G2/M transition checkpoint Ref.2 Inferred from mutant phenotype. Source: SGD intra-S DNA damage checkpoint Ref.2 Inferred from mutant phenotype. Source: SGD meiosis Ref.6 Inferred from mutant phenotype. Source: SGD recombinational repair Inferred from direct assay. Source: SGD
Cellular component	checkpoint clamp complex Ref.3 Ref.5 Ref.13 Inferred from direct assay. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.3 Ref.16 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MEC3	Q02574	1	EBI-30769,EBI-10658
RAD17	P48581	2	EBI-30769,EBI-14652
REV7	P38927	2	EBI-30769,EBI-14960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	DNA damage checkpoint protein 1		PRO_0000239638
Amino acid modifications
	Modified residue	436	1	Phosphoserine Ref.18
	Modified residue	495	1	Phosphoserine Ref.19

Sequences

	Sequence		Length	Mass (Da)
	Q08949-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F9933697C61021D3	FASTA	612	69,694
	10 20 30 40 50 60 MSFKATITES GKQNIWFRAI YVLSTIQDDI KITVTTNELI AWSMNETDTT LCQVRFQKSF 70 80 90 100 110 120 FEEYEFKPHE IVFGENGVQV IEDTYGNSHK LYSFRVNGRH LTTISRKPDG DGIKSFTIAV 130 140 150 160 170 180 NNTSTCPESL ANRLIVVIEM DSLIVKEYCP QFQPIKYDPI IINLKYKRRF LDVFGTAASD 190 200 210 220 230 240 RNPQEPLDPK LLDVFTNTER ELTSALFNEE VESDIRKRNQ LTAADEINYI CCNSTLLKNF 250 260 270 280 290 300 LDNCNVNVTD EVKLEINVHR LSITAFTKAV YGKNNDLLRN ALSMSNTIST LDLEHYCLFT 310 320 330 340 350 360 TIEDEKQDKR SHSKRREHMK SIIFKLKDFK NFITIGPSWK TTQDGNDNIS LWFCHPGDPI 370 380 390 400 410 420 LMQMQKPGVK LELVEVTDSN INDDILEGKF IKTAISGSKE EAGLKDNKES CESPLKSKTA 430 440 450 460 470 480 LKRENLPHSV AGTRNSPLKV SYLTPDNGST VAKTYRNNTA RKLFVEEQSQ STNYEQDKRF 490 500 510 520 530 540 RQASSVHMNM NREQSFDIGT THEVACPRNE SNSLKRSIAD ICNETEDPTQ QSTFAKRADT 550 560 570 580 590 600 TVTWGKALPA ADDEVSCSNI DRKGMLKKEK LKHMQGLLNS QNDTSNHKKQ DNKEMEDGLG 610 LTQVEKPRGI FD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast." Longhese M.P., Paciotti V., Fraschini R., Zaccarini R., Plevani P., Lucchini G. EMBO J. 16:5216-5226(1997) [PubMed: 9311982] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION.
[3]	"Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p." Paciotti V., Lucchini G., Plevani P., Longhese M.P. EMBO J. 17:4199-4209(1998) [PubMed: 9670034] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH MEC3.
[4]	"Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase." Pellicioli A., Lucca C., Liberi G., Marini F., Lopes M., Plevani P., Romano A., Di Fiore P.P., Foiani M. EMBO J. 18:6561-6572(1999) [PubMed: 10562568] [Abstract] Cited for: FUNCTION.
[5]	"Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway." Kondo T., Matsumoto K., Sugimoto K. Mol. Cell. Biol. 19:1136-1143(1999) [PubMed: 9891048] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[6]	"A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint." Hong E.-J.E., Roeder G.S. Genes Dev. 16:363-376(2002) [PubMed: 11825877] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
[7]	"Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway." Wang H., Elledge S.J. Genetics 160:1295-1304(2002) [PubMed: 11973288] [Abstract] Cited for: INTERACTION WITH DPB11.
[8]	"A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1." Giannattasio M., Sommariva E., Vercillo R., Lippi-Boncambi F., Liberi G., Foiani M., Plevani P., Muzi-Falconi M. Proc. Natl. Acad. Sci. U.S.A. 99:12997-13002(2002) [PubMed: 12271137] [Abstract] Cited for: FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[9]	"Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1." Giannattasio M., Sabbioneda S., Minuzzo M., Plevani P., Muzi-Falconi M. J. Biol. Chem. 278:22303-22308(2003) [PubMed: 12672803] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[13]	"Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint." Majka J., Burgers P.M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2249-2254(2003) [PubMed: 12604797] [Abstract] Cited for: INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24 CHECKPOINT CLAMP LOADER COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[14]	"Requirement for ATP by the DNA damage checkpoint clamp loader." Majka J., Chung B.Y., Burgers P.M.J. J. Biol. Chem. 279:20921-20926(2004) [PubMed: 15014082] [Abstract] Cited for: INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24 CHECKPOINT CLAMP LOADER COMPLEX.
[15]	"Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint." Majka J., Burgers P.M.J. DNA Repair 4:1189-1194(2005) [PubMed: 16137930] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[16]	"The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae." Sabbioneda S., Minesinger B.K., Giannattasio M., Plevani P., Muzi-Falconi M., Jinks-Robertson S. J. Biol. Chem. 280:38657-38665(2005) [PubMed: 16169844] [Abstract] Cited for: INTERACTION WITH REV7, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[17]	"Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3." Cardone J.M., Revers L.F., Machado R.M., Bonatto D., Brendel M., Henriques J.A.P. DNA Repair 5:163-171(2006) [PubMed: 16202664] [Abstract] Cited for: INTERACTION WITH MEC3 AND RAD17.
[18]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-436, MASS SPECTROMETRY.
[19]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73550 Genomic DNA. Translation: CAA97907.1.
PIR	S65213.
RefSeq	NP_015130.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2323N.
IntAct	Q08949. 9 interactions.
STRING	Q08949.
Proteomic databases
PeptideAtlas	Q08949.
PRIDE	Q08949.
Genome annotation databases
Ensembl	YPL194W; YPL194W; YPL194W; Saccharomyces cerevisiae. [Genome view]
GeneID	855907.
KEGG	sce:YPL194W.
NMPDR	fig|4932.3.peg.6258.
Organism-specific databases
CYGD	YPL194w.
SGD	S000006115. DDC1.
Phylogenomic databases
HOGENOM	Q08949.
OMA	NTERELT
OrthoDB	EOG9M937V
Gene expression databases
ArrayExpress	Q08949.
Genevestigator	Q08949.
GermOnline	YPL194W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	980607.

Entry information

Entry name

DDC1_YEAST

Accession

Primary (citable) accession number: Q08949

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)