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Protein

FACT complex subunit SSRP1

Gene

Ssrp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA. Also acts as a transcriptional coactivator for p63/TP63.1 Publication

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi547 – 615HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
FACT complex subunit SSRP1
Alternative name(s):
Facilitates chromatin transcription complex subunit SSRP1
Recombination signal sequence recognition protein 1
Structure-specific recognition protein 1
T160
Gene namesi
Name:Ssrp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107912 Ssrp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryos die soon after implantation while preimplantation blastocysts are defective for cell outgrowth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000486072 – 708FACT complex subunit SSRP1Add BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki90Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei170PhosphothreonineBy similarity1
Modified residuei233N6-acetyllysineBy similarity1
Cross-linki296Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki364Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei413N6-acetyllysineBy similarity1
Modified residuei441PhosphotyrosineCombined sources1
Modified residuei444PhosphoserineCombined sources1
Modified residuei452PhosphotyrosineCombined sources1
Modified residuei471PhosphoserineBy similarity1
Modified residuei510Phosphoserine; by CK2By similarity1
Modified residuei542N6-acetyllysineCombined sources1
Modified residuei657Phosphoserine; by CK2By similarity1
Modified residuei659PhosphoserineBy similarity1
Modified residuei667PhosphoserineBy similarity1
Modified residuei668PhosphoserineBy similarity1
Modified residuei671PhosphoserineBy similarity1
Modified residuei672PhosphoserineBy similarity1
Modified residuei673PhosphoserineBy similarity1
Modified residuei688Phosphoserine; by CK2By similarity1

Post-translational modificationi

Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity (By similarity).By similarity
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein (By similarity).By similarity
Sumoylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei450 – 451Cleavage; by caspase-3 and/or caspase-7By similarity2

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ08943
MaxQBiQ08943
PaxDbiQ08943
PeptideAtlasiQ08943
PRIDEiQ08943

PTM databases

iPTMnetiQ08943
PhosphoSitePlusiQ08943
SwissPalmiQ08943

Expressioni

Gene expression databases

BgeeiENSMUSG00000027067
CleanExiMM_SSRP1
ExpressionAtlasiQ08943 baseline and differential
GenevisibleiQ08943 MM

Interactioni

Subunit structurei

Interacts with MYOG (via C-terminal region) (PubMed:23364797). Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Binds to histone H3-H4 tetramers, but not to intact nucleosomes. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Interacts with isoform gamma of TP63. Interacts with FYTTD1/UIF (By similarity). Interacts with SRF (By similarity). Interacts with NEK9 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi2035124 interactors.
IntActiQ08943 5 interactors.
MINTiQ08943
STRINGi10090.ENSMUSP00000076971

Structurei

3D structure databases

ProteinModelPortaliQ08943
SMRiQ08943
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi439 – 496Asp/Glu-rich (acidic)Add BLAST58
Compositional biasi497 – 511Ser-richAdd BLAST15
Compositional biasi512 – 534Arg/Lys-rich (basic)Add BLAST23
Compositional biasi623 – 640Arg/Lys-rich (basic)Add BLAST18
Compositional biasi641 – 708Ser-richAdd BLAST68

Sequence similaritiesi

Belongs to the SSRP1 family.Curated

Phylogenomic databases

eggNOGiKOG0526 Eukaryota
COG5165 LUCA
GeneTreeiENSGT00560000076898
HOGENOMiHOG000180790
HOVERGENiHBG002932
InParanoidiQ08943
KOiK09272
OMAiTGDAICI
OrthoDBiEOG091G04JP
PhylomeDBiQ08943
TreeFamiTF315228

Family and domain databases

Gene3Di1.10.30.101 hit
2.30.29.2201 hit
2.30.29.302 hits
InterProiView protein in InterPro
IPR013719 DUF1747
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR011993 PH-like_dom_sf
IPR035417 POB3_N
IPR000969 SSrcognition
IPR024954 SSRP1_dom
IPR038167 SSRP1_sf
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
PF17292 POB3_N, 1 hit
PF08512 Rtt106, 1 hit
PF03531 SSrecog, 1 hit
PRINTSiPR00887 SSRCOGNITION
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SM01287 Rtt106, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08943-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAETLEFNDI FQEVKGSMND GRLRLSRQGI IFKNSKTGKV DNIQAGELTE
60 70 80 90 100
GIWRRVALGH GLKLLTKNGH VYKYDGFRES EFEKLSDFFK THYRLELMEK
110 120 130 140 150
DLCVKGWNWG TVKFGGQLLS FDIGDQPVFE IPLSNVSQCT TGKNEVTLEF
160 170 180 190 200
HQNDDAEVSL MEVRFYVPPT QEDGVDPVEA FAQNVLSKAD VIQATGDAIC
210 220 230 240 250
IFRELQCLTP RGRYDIRIYP TFLHLHGKTF DYKIPYTTVL RLFLLPHKDQ
260 270 280 290 300
RQMFFVISLD PPIKQGQTRY HFLILLFSKD EDISLTLNMN EEEVEKRFEG
310 320 330 340 350
RLTKNMSGSL YEMVSRVMKA LVNRKITVPG NFQGHSGAQC ITCSYKASSG
360 370 380 390 400
LLYPLERGFI YVHKPPVHIR FDEISFVNFA RGTTTTRSFD FEIETKQGTQ
410 420 430 440 450
YTFSSIEREE YGKLFDFVNA KKLNIKNRGL KEGINPGYDD YADSDEDQHD
460 470 480 490 500
AYLERMKEEG KIREENANDS SDDSGEETDE SFNPGEEEED VAEEFDSNAS
510 520 530 540 550
ASSSSNEGDS DREEKKREQL KRAKMAKDRK SRRKSSEAKK GKDPNAPKRP
560 570 580 590 600
MSAYMLWLNA SREKIKSDHP GISITDLSKK AGEIWKGMSK EKKEEWDRKA
610 620 630 640 650
EDARREYEKA MKEYEGGRGD SSKRDKSKKK KKVKAKMEKK STPSRGSSSK
660 670 680 690 700
SSSRQLSDSF KSKEFVSSDE SSSGENKSKK KRRRSEDSEE ELASTPPSSE

DSASGSDE
Length:708
Mass (Da):80,860
Last modified:July 11, 2006 - v2
Checksum:i8259C1A6E9899843
GO
Isoform 2 (identifier: Q08943-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     621-621: S → SSKRDK

Note: No experimental confirmation available.
Show »
Length:713
Mass (Da):81,475
Checksum:i4216D673573637ED
GO

Sequence cautioni

The sequence AAH42502 differs from that shown. Reason: Frameshift at position 697.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27 – 28RQ → PS in AAB19500 (PubMed:1678855).Curated2
Sequence conflicti54R → P in AAB19500 (PubMed:1678855).Curated1
Sequence conflicti138 – 144QCTTGKN → SVPQARI in AAB19500 (PubMed:1678855).Curated7
Sequence conflicti156A → P in AAB19500 (PubMed:1678855).Curated1
Sequence conflicti589 – 590SK → HE in AAH96682 (PubMed:15489334).Curated2
Sequence conflicti604R → S in AAH96682 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019626621S → SSKRDK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S50213 mRNA Translation: AAB19500.2
AK146446 mRNA Translation: BAE27178.1
AK146585 mRNA Translation: BAE27280.1
AK146607 mRNA Translation: BAE27299.1
AK151584 mRNA Translation: BAE30524.1
AK158552 mRNA Translation: BAE34555.1
BC042502 mRNA Translation: AAH42502.1 Frameshift.
BC096682 mRNA Translation: AAH96682.1
CCDSiCCDS38165.1 [Q08943-1]
PIRiA41265
RefSeqiNP_001129553.1, NM_001136081.2 [Q08943-1]
NP_892035.2, NM_182990.4 [Q08943-1]
XP_006499133.1, XM_006499070.1 [Q08943-1]
UniGeneiMm.219793
Mm.491456

Genome annotation databases

EnsembliENSMUST00000077798; ENSMUSP00000076971; ENSMUSG00000027067 [Q08943-1]
ENSMUST00000168266; ENSMUSP00000127058; ENSMUSG00000027067 [Q08943-1]
GeneIDi20833
KEGGimmu:20833
UCSCiuc008kju.2 mouse [Q08943-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSSRP1_MOUSE
AccessioniPrimary (citable) accession number: Q08943
Secondary accession number(s): Q3U9Z2
, Q3UJ75, Q4V9U4, Q8CGA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2006
Last modified: March 28, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome