Q08932 (RSA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome assembly 1 protein

Gene names

Name:	RSA1
Ordered Locus Names:	YPL193W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
Subcellular location	Nucleus.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ribosomal large subunit assembly Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	nucleoplasm Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Ribosome assembly 1 protein		PRO_0000097460
Amino acid modifications
	Modified residue	172	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q08932 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 16058BA430E0F310	FASTA	381	43,998
	10 20 30 40 50 60 MNYNNFENSK GDGHSRLPKP TYSGTLSDGY DESKIKRQKT DSAFNAAYSP HMYPNSPYYE 70 80 90 100 110 120 GSWNTGYTPQ LHHVAPHNQY FHPIQPSTQY NYTSPPNYTE NYIPPVHQNI SYAPALNLQK 130 140 150 160 170 180 WPSSYCENTQ ALKNDKDYQT SISYEDVAIP TVKEIQLIEK NRGKDTFMNE ISPVPSSKDQ 190 200 210 220 230 240 ASAEPTEIPR KDPELANSNA EDDHNNLGLE DDDRDEQLES EGLGKVVLVP GTSIALITDE 250 260 270 280 290 300 DVKKWREERK KMWLLKISNN KQKHMQEMGI KEDELKSQPS IFKESRKEKQ FIQSIQNQVQ 310 320 330 340 350 360 RGNPKIDLNL KLIQREFANE NSQLLDFIRE LGDVGLLEYE LSQQEKDVLF GSSEDNNKNH 370 380 YKPNYKNRKP NLSRANFTRN K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Synthetic lethality with conditional dbp6 alleles identifies Rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits." Kressler D., Doere M., Rojo M., Linder P. Mol. Cell. Biol. 19:8633-8645(1999) [PubMed: 10567587] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73549 Genomic DNA. Translation: CAA97906.1. BK006949 Genomic DNA. Translation: DAA11241.1.
PIR	S65212.
RefSeq	NP_015131.1. NM_001184007.1.
3D structure databases
ProteinModelPortal	Q08932.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5698N.
IntAct	Q08932. 2 interactions.
MINT	MINT-511275.
STRING	Q08932.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL193W; YPL193W; YPL193W.
GeneID	855908.
KEGG	sce:YPL193W.
NMPDR	fig|4932.3.peg.6259.
Organism-specific databases
CYGD	YPL193w.
SGD	S000006114. RSA1.
Phylogenomic databases
eggNOG	fuNOG10859.
OrthoDB	EOG4G7G84.
Gene expression databases
ArrayExpress	Q08932.
Genevestigator	Q08932.
GermOnline	YPL193W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019496. NUFIP1_cons_dom. [Graphical view]
KO	K14853.
Pfam	PF10453. NUFIP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980610.

Entry information

Entry name

RSA1_YEAST

Accession

Primary (citable) accession number: Q08932
Secondary accession number(s): D6W3H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program