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Q08932 (RSA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome assembly 1 protein
Gene names
Name:RSA1
Ordered Locus Names:YPL193W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.

Subcellular location

Nucleus.

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.

Ontologies

Keywords
   Biological processRibosome biogenesis
   Cellular componentNucleus
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processribosomal large subunit assembly

Inferred from mutant phenotype Ref.3. Source: SGD

   Cellular_componentnucleoplasm

Inferred from direct assay Ref.3. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Ribosome assembly 1 protein
PRO_0000097460

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6
Modified residue1721Phosphoserine Ref.5

Secondary structure

... 381
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q08932 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 16058BA430E0F310

FASTA38143,998
        10         20         30         40         50         60 
MNYNNFENSK GDGHSRLPKP TYSGTLSDGY DESKIKRQKT DSAFNAAYSP HMYPNSPYYE 

        70         80         90        100        110        120 
GSWNTGYTPQ LHHVAPHNQY FHPIQPSTQY NYTSPPNYTE NYIPPVHQNI SYAPALNLQK 

       130        140        150        160        170        180 
WPSSYCENTQ ALKNDKDYQT SISYEDVAIP TVKEIQLIEK NRGKDTFMNE ISPVPSSKDQ 

       190        200        210        220        230        240 
ASAEPTEIPR KDPELANSNA EDDHNNLGLE DDDRDEQLES EGLGKVVLVP GTSIALITDE 

       250        260        270        280        290        300 
DVKKWREERK KMWLLKISNN KQKHMQEMGI KEDELKSQPS IFKESRKEKQ FIQSIQNQVQ 

       310        320        330        340        350        360 
RGNPKIDLNL KLIQREFANE NSQLLDFIRE LGDVGLLEYE LSQQEKDVLF GSSEDNNKNH 

       370        380 
YKPNYKNRKP NLSRANFTRN K 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Synthetic lethality with conditional dbp6 alleles identifies Rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits."
Kressler D., Doere M., Rojo M., Linder P.
Mol. Cell. Biol. 19:8633-8645(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73549 Genomic DNA. Translation: CAA97906.1.
BK006949 Genomic DNA. Translation: DAA11241.1.
PIRS65212.
RefSeqNP_015131.1. NM_001184007.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M3FNMR-A238-259[»]
ProteinModelPortalQ08932.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35990. 20 interactions.
DIPDIP-5698N.
IntActQ08932. 2 interactions.
MINTMINT-511275.
STRING4932.YPL193W.

Proteomic databases

MaxQBQ08932.
PaxDbQ08932.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL193W; YPL193W; YPL193W.
GeneID855908.
KEGGsce:YPL193W.

Organism-specific databases

CYGDYPL193w.
SGDS000006114. RSA1.

Phylogenomic databases

eggNOGNOG38968.
HOGENOMHOG000000983.
KOK14853.
OMASSYCENT.
OrthoDBEOG722JK2.

Enzyme and pathway databases

BioCycYEAST:G3O-34086-MONOMER.

Gene expression databases

GenevestigatorQ08932.

Family and domain databases

InterProIPR019496. NUFIP1_cons_dom.
[Graphical view]
PfamPF10453. NUFIP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980610.

Entry information

Entry nameRSA1_YEAST
AccessionPrimary (citable) accession number: Q08932
Secondary accession number(s): D6W3H5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references