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Q08931 (PRM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pheromone-regulated membrane protein 3
Gene names
Name:PRM3
Ordered Locus Names:YPL192C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length133 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the fusion of nuclear envelopes during mating, ensuring proper karyogamy. Plays a role in the initiation of outer nuclear envelope fusion. Ref.5 Ref.8 Ref.10

Subunit structure

Interacts with KAR5. Ref.8

Subcellular location

Nucleus outer membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body Ref.5 Ref.6 Ref.7 Ref.8 Ref.9.

Induction

By pheromone. Expression is controlled by the STE12 transcription factor that binds to pheromone-response cis-elements (PREs) in the promoter of target genes. Ref.4 Ref.11

Caution

Was originally shown to be localized in the inner nuclear membrane by using a truncated protein (Ref.5). Was used as an inner nuclear membrane marker (Ref.6, Ref.7 and Ref.9). However, more recent studies showed that it is localized in the nuclear outer membrane, which is consistent with a function in the initiation of nuclear fusion (Ref.8 and Ref.10).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 133133Pheromone-regulated membrane protein 3
PRO_0000262749

Regions

Topological domain1 – 104104Nuclear Potential
Transmembrane105 – 12723Helical; Potential
Topological domain128 – 1336Perinuclear space Potential
Motif69 – 757Bipartite nuclear localization signal

Experimental info

Mutagenesis1061Y → H in pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises. Ref.8
Mutagenesis1081G → S in pmr3-1; decreases nuclear fusion efficiency. Ref.8
Mutagenesis1121G → S in pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8
Mutagenesis1141F → S in pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8
Mutagenesis1151L → R in pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8
Mutagenesis1211T → A in pmr3-6; leads to mislocalization. Ref.8
Mutagenesis1221V → D in pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q08931 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EEC7EC604F06C2B1

FASTA13314,428
        10         20         30         40         50         60 
MTAMKEDNAA LITLKKNNDQ EKLRVHKLTD ASSNSADGFV INKAKNGGPL NKKSLVNNEQ 

        70         80         90        100        110        120 
HIKKAVSPGR VRKHKTTTSS TKSRTKSKKK DASESKVQRE NKGSFYQGAI FGSFLGAAVT 

       130 
TVLSNLAVKA LQN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating."
Heiman M.G., Walter P.
J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, INDUCTION.
[5]"Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae."
Beilharz T., Egan B., Silver P.A., Hofmann K., Lithgow T.
J. Biol. Chem. 278:8219-8223(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Immobility, inheritance and plasticity of shape of the yeast nucleus."
Hattier T., Andrulis E.D., Tartakoff A.M.
BMC Cell Biol. 8:47-47(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"A mechanism for asymmetric segregation of age during yeast budding."
Shcheprova Z., Baldi S., Frei S.B., Gonnet G., Barral Y.
Nature 454:728-734(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion."
Shen S., Tobery C.E., Rose M.D.
Mol. Biol. Cell 20:2438-2450(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR5, MUTAGENESIS OF TYR-106; GLY-108; GLY-112; PHE-114; LEU-115; THR-121 AND VAL-122.
[9]"Nuclear fusion and genome encounter during yeast zygote formation."
Tartakoff A.M., Jaiswal P.
Mol. Biol. Cell 20:2932-2942(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Distinct roles for key karyogamy proteins during yeast nuclear fusion."
Melloy P., Shen S., White E., Rose M.D.
Mol. Biol. Cell 20:3773-3782(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Organizational constraints on Ste12 cis-elements for a pheromone response in Saccharomyces cerevisiae."
Su T.C., Tamarkina E., Sadowski I.
FEBS J. 277:3235-3248(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73548 Genomic DNA. Translation: CAA97905.1.
AY693130 Genomic DNA. Translation: AAT93149.1.
BK006949 Genomic DNA. Translation: DAA11242.1.
PIRS65211.
RefSeqNP_015132.1. NM_001184006.1.

3D structure databases

ProteinModelPortalQ08931.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35991. 21 interactions.
DIPDIP-1699N.
IntActQ08931. 7 interactions.
MINTMINT-386668.
STRING4932.YPL192C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL192C; YPL192C; YPL192C.
GeneID855909.
KEGGsce:YPL192C.

Organism-specific databases

CYGDYPL192c.
SGDS000006113. PRM3.

Phylogenomic databases

OrthoDBEOG7K9KG4.

Enzyme and pathway databases

BioCycYEAST:G3O-34085-MONOMER.

Gene expression databases

GenevestigatorQ08931.

Family and domain databases

ProtoNetSearch...

Other

NextBio980613.

Entry information

Entry namePRM3_YEAST
AccessionPrimary (citable) accession number: Q08931
Secondary accession number(s): D6W3H6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD