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Q08931

- PRM3_YEAST

UniProt

Q08931 - PRM3_YEAST

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Protein

Pheromone-regulated membrane protein 3

Gene

PRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for the fusion of nuclear envelopes during mating, ensuring proper karyogamy. Plays a role in the initiation of outer nuclear envelope fusion.3 Publications

GO - Biological processi

  1. karyogamy involved in conjugation with cellular fusion Source: SGD
  2. nuclear membrane fusion involved in karyogamy Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-34085-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-regulated membrane protein 3
Gene namesi
Name:PRM3
Ordered Locus Names:YPL192C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL192c.
SGDiS000006113. PRM3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 104104NuclearSequence AnalysisAdd
BLAST
Transmembranei105 – 12723HelicalSequence AnalysisAdd
BLAST
Topological domaini128 – 1336Perinuclear spaceSequence Analysis

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extrinsic component of nuclear outer membrane Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. nuclear envelope Source: SGD
  5. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1061Y → H in pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises. 1 Publication
Mutagenesisi108 – 1081G → S in pmr3-1; decreases nuclear fusion efficiency. 1 Publication
Mutagenesisi112 – 1121G → S in pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication
Mutagenesisi114 – 1141F → S in pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication
Mutagenesisi115 – 1151L → R in pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication
Mutagenesisi121 – 1211T → A in pmr3-6; leads to mislocalization. 1 Publication
Mutagenesisi122 – 1221V → D in pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 133133Pheromone-regulated membrane protein 3PRO_0000262749Add
BLAST

Expressioni

Inductioni

By pheromone. Expression is controlled by the STE12 transcription factor that binds to pheromone-response cis-elements (PREs) in the promoter of target genes.2 Publications

Gene expression databases

GenevestigatoriQ08931.

Interactioni

Subunit structurei

Interacts with KAR5.1 Publication

Protein-protein interaction databases

BioGridi35991. 21 interactions.
DIPiDIP-1699N.
IntActiQ08931. 7 interactions.
MINTiMINT-386668.
STRINGi4932.YPL192C.

Structurei

3D structure databases

ProteinModelPortaliQ08931.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 757Bipartite nuclear localization signal

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ08931.
OrthoDBiEOG7K9KG4.

Sequencei

Sequence statusi: Complete.

Q08931-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAMKEDNAA LITLKKNNDQ EKLRVHKLTD ASSNSADGFV INKAKNGGPL
60 70 80 90 100
NKKSLVNNEQ HIKKAVSPGR VRKHKTTTSS TKSRTKSKKK DASESKVQRE
110 120 130
NKGSFYQGAI FGSFLGAAVT TVLSNLAVKA LQN
Length:133
Mass (Da):14,428
Last modified:November 1, 1996 - v1
Checksum:iEEC7EC604F06C2B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73548 Genomic DNA. Translation: CAA97905.1.
AY693130 Genomic DNA. Translation: AAT93149.1.
BK006949 Genomic DNA. Translation: DAA11242.1.
PIRiS65211.
RefSeqiNP_015132.1. NM_001184006.1.

Genome annotation databases

EnsemblFungiiYPL192C; YPL192C; YPL192C.
GeneIDi855909.
KEGGisce:YPL192C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73548 Genomic DNA. Translation: CAA97905.1 .
AY693130 Genomic DNA. Translation: AAT93149.1 .
BK006949 Genomic DNA. Translation: DAA11242.1 .
PIRi S65211.
RefSeqi NP_015132.1. NM_001184006.1.

3D structure databases

ProteinModelPortali Q08931.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35991. 21 interactions.
DIPi DIP-1699N.
IntActi Q08931. 7 interactions.
MINTi MINT-386668.
STRINGi 4932.YPL192C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL192C ; YPL192C ; YPL192C .
GeneIDi 855909.
KEGGi sce:YPL192C.

Organism-specific databases

CYGDi YPL192c.
SGDi S000006113. PRM3.

Phylogenomic databases

InParanoidi Q08931.
OrthoDBi EOG7K9KG4.

Enzyme and pathway databases

BioCyci YEAST:G3O-34085-MONOMER.

Miscellaneous databases

NextBioi 980613.

Gene expression databases

Genevestigatori Q08931.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating."
    Heiman M.G., Walter P.
    J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, INDUCTION.
  5. "Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae."
    Beilharz T., Egan B., Silver P.A., Hofmann K., Lithgow T.
    J. Biol. Chem. 278:8219-8223(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Immobility, inheritance and plasticity of shape of the yeast nucleus."
    Hattier T., Andrulis E.D., Tartakoff A.M.
    BMC Cell Biol. 8:47-47(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "A mechanism for asymmetric segregation of age during yeast budding."
    Shcheprova Z., Baldi S., Frei S.B., Gonnet G., Barral Y.
    Nature 454:728-734(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion."
    Shen S., Tobery C.E., Rose M.D.
    Mol. Biol. Cell 20:2438-2450(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR5, MUTAGENESIS OF TYR-106; GLY-108; GLY-112; PHE-114; LEU-115; THR-121 AND VAL-122.
  9. "Nuclear fusion and genome encounter during yeast zygote formation."
    Tartakoff A.M., Jaiswal P.
    Mol. Biol. Cell 20:2932-2942(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Distinct roles for key karyogamy proteins during yeast nuclear fusion."
    Melloy P., Shen S., White E., Rose M.D.
    Mol. Biol. Cell 20:3773-3782(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Organizational constraints on Ste12 cis-elements for a pheromone response in Saccharomyces cerevisiae."
    Su T.C., Tamarkina E., Sadowski I.
    FEBS J. 277:3235-3248(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPRM3_YEAST
AccessioniPrimary (citable) accession number: Q08931
Secondary accession number(s): D6W3H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally shown to be localized in the inner nuclear membrane by using a truncated protein (PubMed:12514182). Was used as an inner nuclear membrane marker (PubMed:17996101, PubMed:18660802 and PubMed:19369416). However, more recent studies showed that it is localized in the nuclear outer membrane, which is consistent with a function in the initiation of nuclear fusion (PubMed:19297527 and PubMed:19570912).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3