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Q08931

- PRM3_YEAST

UniProt

Q08931 - PRM3_YEAST

Protein

Pheromone-regulated membrane protein 3

Gene

PRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Required for the fusion of nuclear envelopes during mating, ensuring proper karyogamy. Plays a role in the initiation of outer nuclear envelope fusion.3 Publications

    GO - Biological processi

    1. karyogamy involved in conjugation with cellular fusion Source: SGD
    2. nuclear membrane fusion involved in karyogamy Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34085-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pheromone-regulated membrane protein 3
    Gene namesi
    Name:PRM3
    Ordered Locus Names:YPL192C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL192c.
    SGDiS000006113. PRM3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. extrinsic component of nuclear outer membrane Source: SGD
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear envelope Source: SGD
    5. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi106 – 1061Y → H in pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises. 1 Publication
    Mutagenesisi108 – 1081G → S in pmr3-1; decreases nuclear fusion efficiency. 1 Publication
    Mutagenesisi112 – 1121G → S in pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication
    Mutagenesisi114 – 1141F → S in pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication
    Mutagenesisi115 – 1151L → R in pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication
    Mutagenesisi121 – 1211T → A in pmr3-6; leads to mislocalization. 1 Publication
    Mutagenesisi122 – 1221V → D in pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 133133Pheromone-regulated membrane protein 3PRO_0000262749Add
    BLAST

    Expressioni

    Inductioni

    By pheromone. Expression is controlled by the STE12 transcription factor that binds to pheromone-response cis-elements (PREs) in the promoter of target genes.2 Publications

    Gene expression databases

    GenevestigatoriQ08931.

    Interactioni

    Subunit structurei

    Interacts with KAR5.1 Publication

    Protein-protein interaction databases

    BioGridi35991. 21 interactions.
    DIPiDIP-1699N.
    IntActiQ08931. 7 interactions.
    MINTiMINT-386668.
    STRINGi4932.YPL192C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08931.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 104104NuclearSequence AnalysisAdd
    BLAST
    Topological domaini128 – 1336Perinuclear spaceSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei105 – 12723HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi69 – 757Bipartite nuclear localization signal

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    OrthoDBiEOG7K9KG4.

    Sequencei

    Sequence statusi: Complete.

    Q08931-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAMKEDNAA LITLKKNNDQ EKLRVHKLTD ASSNSADGFV INKAKNGGPL    50
    NKKSLVNNEQ HIKKAVSPGR VRKHKTTTSS TKSRTKSKKK DASESKVQRE 100
    NKGSFYQGAI FGSFLGAAVT TVLSNLAVKA LQN 133
    Length:133
    Mass (Da):14,428
    Last modified:November 1, 1996 - v1
    Checksum:iEEC7EC604F06C2B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73548 Genomic DNA. Translation: CAA97905.1.
    AY693130 Genomic DNA. Translation: AAT93149.1.
    BK006949 Genomic DNA. Translation: DAA11242.1.
    PIRiS65211.
    RefSeqiNP_015132.1. NM_001184006.1.

    Genome annotation databases

    EnsemblFungiiYPL192C; YPL192C; YPL192C.
    GeneIDi855909.
    KEGGisce:YPL192C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73548 Genomic DNA. Translation: CAA97905.1 .
    AY693130 Genomic DNA. Translation: AAT93149.1 .
    BK006949 Genomic DNA. Translation: DAA11242.1 .
    PIRi S65211.
    RefSeqi NP_015132.1. NM_001184006.1.

    3D structure databases

    ProteinModelPortali Q08931.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35991. 21 interactions.
    DIPi DIP-1699N.
    IntActi Q08931. 7 interactions.
    MINTi MINT-386668.
    STRINGi 4932.YPL192C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL192C ; YPL192C ; YPL192C .
    GeneIDi 855909.
    KEGGi sce:YPL192C.

    Organism-specific databases

    CYGDi YPL192c.
    SGDi S000006113. PRM3.

    Phylogenomic databases

    OrthoDBi EOG7K9KG4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34085-MONOMER.

    Miscellaneous databases

    NextBioi 980613.

    Gene expression databases

    Genevestigatori Q08931.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating."
      Heiman M.G., Walter P.
      J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, INDUCTION.
    5. "Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae."
      Beilharz T., Egan B., Silver P.A., Hofmann K., Lithgow T.
      J. Biol. Chem. 278:8219-8223(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Immobility, inheritance and plasticity of shape of the yeast nucleus."
      Hattier T., Andrulis E.D., Tartakoff A.M.
      BMC Cell Biol. 8:47-47(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "A mechanism for asymmetric segregation of age during yeast budding."
      Shcheprova Z., Baldi S., Frei S.B., Gonnet G., Barral Y.
      Nature 454:728-734(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion."
      Shen S., Tobery C.E., Rose M.D.
      Mol. Biol. Cell 20:2438-2450(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR5, MUTAGENESIS OF TYR-106; GLY-108; GLY-112; PHE-114; LEU-115; THR-121 AND VAL-122.
    9. "Nuclear fusion and genome encounter during yeast zygote formation."
      Tartakoff A.M., Jaiswal P.
      Mol. Biol. Cell 20:2932-2942(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "Distinct roles for key karyogamy proteins during yeast nuclear fusion."
      Melloy P., Shen S., White E., Rose M.D.
      Mol. Biol. Cell 20:3773-3782(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Organizational constraints on Ste12 cis-elements for a pheromone response in Saccharomyces cerevisiae."
      Su T.C., Tamarkina E., Sadowski I.
      FEBS J. 277:3235-3248(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiPRM3_YEAST
    AccessioniPrimary (citable) accession number: Q08931
    Secondary accession number(s): D6W3H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally shown to be localized in the inner nuclear membrane by using a truncated protein (PubMed:12514182). Was used as an inner nuclear membrane marker (PubMed:17996101, PubMed:18660802 and PubMed:19369416). However, more recent studies showed that it is localized in the nuclear outer membrane, which is consistent with a function in the initiation of nuclear fusion (PubMed:19297527 and PubMed:19570912).1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3