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Protein

Pheromone-regulated membrane protein 3

Gene

PRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the fusion of nuclear envelopes during mating, ensuring proper karyogamy. Plays a role in the initiation of outer nuclear envelope fusion.3 Publications

Caution

Was originally shown to be localized in the inner nuclear membrane by using a truncated protein (PubMed:12514182). Was used as an inner nuclear membrane marker (PubMed:17996101, PubMed:18660802 and PubMed:19369416). However, more recent studies showed that it is localized in the nuclear outer membrane, which is consistent with a function in the initiation of nuclear fusion (PubMed:19297527 and PubMed:19570912).1 Publication

GO - Biological processi

  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • nuclear membrane fusion involved in karyogamy Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-34085-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-regulated membrane protein 3
Gene namesi
Name:PRM3
Ordered Locus Names:YPL192C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL192C
SGDiS000006113 PRM3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 104NuclearSequence analysisAdd BLAST104
Transmembranei105 – 127HelicalSequence analysisAdd BLAST23
Topological domaini128 – 133Perinuclear spaceSequence analysis6

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106Y → H in pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises. 1 Publication1
Mutagenesisi108G → S in pmr3-1; decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi112G → S in pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi114F → S in pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi115L → R in pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi121T → A in pmr3-6; leads to mislocalization. 1 Publication1
Mutagenesisi122V → D in pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627491 – 133Pheromone-regulated membrane protein 3Add BLAST133

Proteomic databases

PaxDbiQ08931

Expressioni

Inductioni

By pheromone. Expression is controlled by the STE12 transcription factor that binds to pheromone-response cis-elements (PREs) in the promoter of target genes.2 Publications

Interactioni

Subunit structurei

Interacts with KAR5.1 Publication

Protein-protein interaction databases

BioGridi35991, 54 interactors
DIPiDIP-1699N
IntActiQ08931, 7 interactors
MINTiQ08931
STRINGi4932.YPL192C

Structurei

3D structure databases

ProteinModelPortaliQ08931
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 75Bipartite nuclear localization signal7

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ08931
OrthoDBiEOG092C5LAH

Sequencei

Sequence statusi: Complete.

Q08931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAMKEDNAA LITLKKNNDQ EKLRVHKLTD ASSNSADGFV INKAKNGGPL
60 70 80 90 100
NKKSLVNNEQ HIKKAVSPGR VRKHKTTTSS TKSRTKSKKK DASESKVQRE
110 120 130
NKGSFYQGAI FGSFLGAAVT TVLSNLAVKA LQN
Length:133
Mass (Da):14,428
Last modified:November 1, 1996 - v1
Checksum:iEEC7EC604F06C2B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73548 Genomic DNA Translation: CAA97905.1
AY693130 Genomic DNA Translation: AAT93149.1
BK006949 Genomic DNA Translation: DAA11242.1
PIRiS65211
RefSeqiNP_015132.1, NM_001184006.1

Genome annotation databases

EnsemblFungiiYPL192C; YPL192C; YPL192C
GeneIDi855909
KEGGisce:YPL192C

Similar proteinsi

Entry informationi

Entry nameiPRM3_YEAST
AccessioniPrimary (citable) accession number: Q08931
Secondary accession number(s): D6W3H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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