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Protein

Pheromone-regulated membrane protein 3

Gene

PRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the fusion of nuclear envelopes during mating, ensuring proper karyogamy. Plays a role in the initiation of outer nuclear envelope fusion.3 Publications

GO - Biological processi

  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • nuclear membrane fusion involved in karyogamy Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-34085-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-regulated membrane protein 3
Gene namesi
Name:PRM3
Ordered Locus Names:YPL192C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL192C.
SGDiS000006113. PRM3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 104NuclearSequence analysisAdd BLAST104
Transmembranei105 – 127HelicalSequence analysisAdd BLAST23
Topological domaini128 – 133Perinuclear spaceSequence analysis6

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • extrinsic component of nuclear outer membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • nuclear envelope Source: SGD
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106Y → H in pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises. 1 Publication1
Mutagenesisi108G → S in pmr3-1; decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi112G → S in pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi114F → S in pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi115L → R in pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1
Mutagenesisi121T → A in pmr3-6; leads to mislocalization. 1 Publication1
Mutagenesisi122V → D in pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627491 – 133Pheromone-regulated membrane protein 3Add BLAST133

Proteomic databases

PRIDEiQ08931.

Expressioni

Inductioni

By pheromone. Expression is controlled by the STE12 transcription factor that binds to pheromone-response cis-elements (PREs) in the promoter of target genes.2 Publications

Interactioni

Subunit structurei

Interacts with KAR5.1 Publication

Protein-protein interaction databases

BioGridi35991. 22 interactors.
DIPiDIP-1699N.
IntActiQ08931. 7 interactors.
MINTiMINT-386668.

Structurei

3D structure databases

ProteinModelPortaliQ08931.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 75Bipartite nuclear localization signal7

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ08931.
OrthoDBiEOG092C5LAH.

Sequencei

Sequence statusi: Complete.

Q08931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAMKEDNAA LITLKKNNDQ EKLRVHKLTD ASSNSADGFV INKAKNGGPL
60 70 80 90 100
NKKSLVNNEQ HIKKAVSPGR VRKHKTTTSS TKSRTKSKKK DASESKVQRE
110 120 130
NKGSFYQGAI FGSFLGAAVT TVLSNLAVKA LQN
Length:133
Mass (Da):14,428
Last modified:November 1, 1996 - v1
Checksum:iEEC7EC604F06C2B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73548 Genomic DNA. Translation: CAA97905.1.
AY693130 Genomic DNA. Translation: AAT93149.1.
BK006949 Genomic DNA. Translation: DAA11242.1.
PIRiS65211.
RefSeqiNP_015132.1. NM_001184006.1.

Genome annotation databases

EnsemblFungiiYPL192C; YPL192C; YPL192C.
GeneIDi855909.
KEGGisce:YPL192C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73548 Genomic DNA. Translation: CAA97905.1.
AY693130 Genomic DNA. Translation: AAT93149.1.
BK006949 Genomic DNA. Translation: DAA11242.1.
PIRiS65211.
RefSeqiNP_015132.1. NM_001184006.1.

3D structure databases

ProteinModelPortaliQ08931.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35991. 22 interactors.
DIPiDIP-1699N.
IntActiQ08931. 7 interactors.
MINTiMINT-386668.

Proteomic databases

PRIDEiQ08931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL192C; YPL192C; YPL192C.
GeneIDi855909.
KEGGisce:YPL192C.

Organism-specific databases

EuPathDBiFungiDB:YPL192C.
SGDiS000006113. PRM3.

Phylogenomic databases

InParanoidiQ08931.
OrthoDBiEOG092C5LAH.

Enzyme and pathway databases

BioCyciYEAST:G3O-34085-MONOMER.

Miscellaneous databases

PROiQ08931.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiPRM3_YEAST
AccessioniPrimary (citable) accession number: Q08931
Secondary accession number(s): D6W3H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally shown to be localized in the inner nuclear membrane by using a truncated protein (PubMed:12514182). Was used as an inner nuclear membrane marker (PubMed:17996101, PubMed:18660802 and PubMed:19369416). However, more recent studies showed that it is localized in the nuclear outer membrane, which is consistent with a function in the initiation of nuclear fusion (PubMed:19297527 and PubMed:19570912).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.