Pheromone-regulated membrane protein 3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q08931 (PRM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pheromone-regulated membrane protein 3

Gene names

Name:	PRM3
Ordered Locus Names:	YPL192C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the fusion of nuclear envelopes during mating, ensuring proper karyogamy. Plays a role in the initiation of outer nuclear envelope fusion. Ref.5 Ref.8 Ref.10
Subunit structure	Interacts with KAR5. Ref.8
Subcellular location	Nucleus outer membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton › spindle pole body Ref.5 Ref.6 Ref.7 Ref.8 Ref.9.
Induction	By pheromone. Expression is controlled by the STE12 transcription factor that binds to pheromone-response cis-elements (PREs) in the promoter of target genes. Ref.4 Ref.11
Caution	Was originally shown to be localized in the inner nuclear membrane by using a truncated protein (Ref.5). Was used as an inner nuclear membrane marker (Ref.6, Ref.7 and Ref.9). However, more recent studies showed that it is localized in the nuclear outer membrane, which is consistent with a function in the initiation of nuclear fusion (Ref.8 and Ref.10).

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	karyogamy involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.5 Ref.8. Source: SGD nuclear membrane fusion involved in karyogamy Inferred from mutant phenotype Ref.8. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to nuclear outer membrane Inferred from direct assay Ref.8. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW spindle pole body Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 133

133

Pheromone-regulated membrane protein 3

PRO_0000262749

Regions

Topological domain

1 – 104

104

Nuclear Potential

Transmembrane

105 – 127

Helical; Potential

Topological domain

128 – 133

Perinuclear space Potential

Motif

69 – 75

Bipartite nuclear localization signal

Experimental info

Mutagenesis

106

Y → H in pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises. Ref.8

Mutagenesis

108

G → S in pmr3-1; decreases nuclear fusion efficiency. Ref.8

Mutagenesis

112

G → S in pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8

Mutagenesis

114

F → S in pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8

Mutagenesis

115

L → R in pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8

Mutagenesis

121

T → A in pmr3-6; leads to mislocalization. Ref.8

Mutagenesis

122

V → D in pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency. Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

Q08931 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EEC7EC604F06C2B1
Show »

FASTA

133

14,428

        10         20         30         40         50         60 
MTAMKEDNAA LITLKKNNDQ EKLRVHKLTD ASSNSADGFV INKAKNGGPL NKKSLVNNEQ 

        70         80         90        100        110        120 
HIKKAVSPGR VRKHKTTTSS TKSRTKSKKK DASESKVQRE NKGSFYQGAI FGSFLGAAVT 

       130 
TVLSNLAVKA LQN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating." Heiman M.G., Walter P. J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, INDUCTION.
[5]	"Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae." Beilharz T., Egan B., Silver P.A., Hofmann K., Lithgow T. J. Biol. Chem. 278:8219-8223(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Immobility, inheritance and plasticity of shape of the yeast nucleus." Hattier T., Andrulis E.D., Tartakoff A.M. BMC Cell Biol. 8:47-47(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"A mechanism for asymmetric segregation of age during yeast budding." Shcheprova Z., Baldi S., Frei S.B., Gonnet G., Barral Y. Nature 454:728-734(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion." Shen S., Tobery C.E., Rose M.D. Mol. Biol. Cell 20:2438-2450(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR5, MUTAGENESIS OF TYR-106; GLY-108; GLY-112; PHE-114; LEU-115; THR-121 AND VAL-122.
[9]	"Nuclear fusion and genome encounter during yeast zygote formation." Tartakoff A.M., Jaiswal P. Mol. Biol. Cell 20:2932-2942(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Distinct roles for key karyogamy proteins during yeast nuclear fusion." Melloy P., Shen S., White E., Rose M.D. Mol. Biol. Cell 20:3773-3782(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Organizational constraints on Ste12 cis-elements for a pheromone response in Saccharomyces cerevisiae." Su T.C., Tamarkina E., Sadowski I. FEBS J. 277:3235-3248(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73548 Genomic DNA. Translation: CAA97905.1. AY693130 Genomic DNA. Translation: AAT93149.1. BK006949 Genomic DNA. Translation: DAA11242.1.
PIR	S65211.
RefSeq	NP_015132.1. NM_001184006.1.
3D structure databases
ProteinModelPortal	Q08931.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1699N.
IntAct	Q08931. 7 interactions.
MINT	MINT-386668.
STRING	4932.YPL192C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL192C; YPL192C; YPL192C.
GeneID	855909.
KEGG	sce:YPL192C.
Organism-specific databases
CYGD	YPL192c.
SGD	S000006113. PRM3.
Gene expression databases
Genevestigator	Q08931.
GermOnline	YPL192C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	980613.

Entry information

Entry name

PRM3_YEAST

Accession

Primary (citable) accession number: Q08931
Secondary accession number(s): D6W3H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents