Q08929 (GUP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol uptake protein 2

Gene names

Name:	GUP2
Ordered Locus Names:	YPL189W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in active uptake of glycerol driven by electrogenic proton symport. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the membrane-bound acyltransferase family.

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol transport Inferred from genetic interaction Ref.3. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Glycerol uptake protein 2		PRO_0000213133
Regions
	Transmembrane	76 – 95	20	Helical; Potential
	Transmembrane	129 – 151	23	Helical; Potential
	Transmembrane	164 – 186	23	Helical; Potential
	Transmembrane	201 – 218	18	Helical; Potential
	Transmembrane	323 – 345	23	Helical; Potential
	Transmembrane	365 – 387	23	Helical; Potential
	Transmembrane	408 – 427	20	Helical; Potential
	Transmembrane	501 – 523	23	Helical; Potential
	Transmembrane	535 – 557	23	Helical; Potential
	Transmembrane	577 – 594	18	Helical; Potential
Sites
	Active site	496	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q08929 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 346B7B9D7E3E5830	FASTA	609	71,289
	10 20 30 40 50 60 MSMLRIWSCI VHFFSVQALD SRIKPDIEFK RRQRIFINSS KEENGSSSSA VTVTRNPVLS 70 80 90 100 110 120 SNSPSPPLWN TWEFRLYYLA FTVVVPFMIK AALATSSESN PNYYKFSGLL AHGWILGRKV 130 140 150 160 170 180 DNSDPQYRFF RSNFFLLAIL ILLQIILKKV FVKFSKIPKT KFDFACGLVF VCFMYGINSV 190 200 210 220 230 240 KLFTHAFIFF TLAHSLKRKR LIAAFAIWSY GIFTLFINQK MKNLPFNNIA IILSPMDQWY 250 260 270 280 290 300 KGIVPRWDFF FNFTLLRLLS YSMDFLERWH EQLSRQPSID YDDRRPEFRK SLSGSTLQTI 310 320 330 340 350 360 YESGKNVLEE KERLVAEHHI QDYNFINFIA YITYAPLFLV GPIITFNDYL YQSENKLPSL 370 380 390 400 410 420 TKKNIGFYAL KVFSSLLLME IILHYIYVGA IARTKAWNND TPLQQAMIAL FNLNIMYLKL 430 440 450 460 470 480 LIPWRLFRLW AMVDGIDAPE NMLRCVDNNY STVGFWRAWH TSFNKWVIRY IYVPFGGSNN 490 500 510 520 530 540 KILTSFAVFS FVAIWHDIQL RVLFWGWLTV LLLLGETYIT NCFSRYRFRS WYRFVCGIGA 550 560 570 580 590 600 AINICMMMII NVYGFCLGAE GTKLLLKGIF NNSHSPEFLT AVMVSLFIAV QVMFEIREEE KRHGINLKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae." Holst B., Lunde C., Lages F., Oliveira R., Lucas C., Kielland-Brandt M.C. Mol. Microbiol. 37:108-124(2000) [PubMed: 10931309] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73545 Genomic DNA. Translation: CAA97902.1. Z73544 Genomic DNA. Translation: CAA97901.1. BK006949 Genomic DNA. Translation: DAA11246.1.
PIR	S65208.
RefSeq	NP_015135.1. NM_001184003.1.
3D structure databases
ProteinModelPortal	Q08929.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8868N.
MINT	MINT-1357821.
STRING	Q08929.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL189W; YPL189W; YPL189W.
GeneID	855912.
KEGG	sce:YPL189W.
NMPDR	fig|4932.3.peg.6264.
Organism-specific databases
CYGD	YPL189w.
SGD	S000006110. GUP2.
Phylogenomic databases
eggNOG	fuNOG04276.
GeneTree	EFGT00050000004859.
HOGENOM	HBG326114.
OMA	FLERWHE.
OrthoDB	EOG4MD17Q.
Gene expression databases
ArrayExpress	Q08929.
Genevestigator	Q08929.
GermOnline	YPL189W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980622.

Entry information

Entry name

GUP2_YEAST

Accession

Primary (citable) accession number: Q08929
Secondary accession number(s): D6W3I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program