Q08923 (CTI6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone deacetylase complex subunit CTI6

Alternative name(s):
CYC8-TUP1-interacting protein 6
Transcriptional regulatory protein CTI6

Gene names

Name:	CTI6
Synonyms:	RXT1
Ordered Locus Names:	YPL181W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. CTI6 links the SAGA coactivator to the CYC8-TUP1 corepressor. Involved in transcription regulation of heme-regulated genes and required for GCN5 recruitment, histone H3 acetylation and SPT15/TBP binding to promoters. Ref.4 Ref.8 Ref.11
Subunit structure	Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Interacts with CYC8. Ref.4 Ref.5
Subcellular location	Nucleus Ref.4 Ref.6 Ref.8.
Induction	Under low iron conditions. Ref.8
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 1 PHD-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of chromatin silencing at rDNA Inferred from mutant phenotype Ref.10. Source: SGD negative regulation of chromatin silencing at silent mating-type cassette Inferred from mutant phenotype Ref.10. Source: SGD negative regulation of chromatin silencing at telomere Inferred from mutant phenotype Ref.10. Source: SGD regulation of DNA-dependent DNA replication initiation Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Rpd3L complex Inferred from direct assay Ref.10Ref.9. Source: SGD Rpd3L-Expanded complex Inferred from direct assay. Source: SGD
Molecular function	methylated histone residue binding Inferred from direct assay. Source: SGD transcription factor binding Inferred from direct assay Ref.4. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Histone deacetylase complex subunit CTI6		PRO_0000234079
Regions
	Zinc finger	72 – 123	52	PHD-type
Amino acid modifications
	Modified residue	170	1	Phosphoserine Ref.12
	Modified residue	216	1	Phosphoserine Ref.12 Ref.13
	Modified residue	364	1	Phosphoserine Ref.13
	Modified residue	441	1	Phosphoserine Ref.13
Experimental info
	Mutagenesis	95	1	C → A: Impairs growth under iron-limiting conditions. Ref.8
	Mutagenesis	100	1	H → A: Impairs growth under iron-limiting conditions. Ref.8
	Sequence conflict	342	1	S → P in AAT92976. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q08923 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5123C56BB86ED991	FASTA	506	57,053
	10 20 30 40 50 60 MESTAIVPKG PVVGSEDMEK AEVIASGSTD IISTTSTATT TAAIGSVQEE SVKQEDVPME 70 80 90 100 110 120 GGEGEVEEEE GETRCICGEL DTPDDSGFFI QCEQCSSWQH GYCVSITQDN APDKYWCEQC 130 140 150 160 170 180 RPELHQLFTT DTGEARSIYK PVQEKRRQSR RKARSAAASK SHAANEAEKS PRNTSNTDDN 190 200 210 220 230 240 VDDIGDEEDE VEDEASAVAL AKDGNTRSSR RRRRNSMDDA STDQYSLDPG DSDKKLLDRK 250 260 270 280 290 300 RATFMAREEK QYQRMLEKAL KESRRTSHQE DPESYENDAD IYQGDTDNHN GTTRLQTDVM 310 320 330 340 350 360 LTEGKPDSVT NDDMKESLRP SKEQSMEKTN DVEKEASQEK ESSTGSAQDT EKTDEPILPL 370 380 390 400 410 420 TSISSSEDDS RKASSRGSKR VSKPARKGNR TRRSNTSSDT NQNRRSADIG TDKPVKPRLP 430 440 450 460 470 480 PQRTSLNEMR RRVSAILEFI SRTQWELSED QSDREEFVRF VENQHFVEKV DTIYNGYNES 490 500 LSMMDDLTRE LLLWEKKYSN NTNAIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1." Papamichos-Chronakis M., Petrakis T., Ktistaki E., Topalidou I., Tzamarias D. Mol. Cell 9:1297-1305(2002) [PubMed: 12086626] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYC8.
[5]	"Functional organization of the yeast proteome by systematic analysis of protein complexes." Gavin A.-C., Boesche M., Krause R., Grandi P., Marzioch M., Bauer A., Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M., Hoefert C., Schelder M., Brajenovic M., Ruffner H., Merino A., Klein K., Hudak M. , Dickson D., Rudi T., Gnau V., Bauch A., Bastuck S., Huhse B., Leutwein C., Heurtier M.-A., Copley R.R., Edelmann A., Querfurth E., Rybin V., Drewes G., Raida M., Bouwmeester T., Bork P., Seraphin B., Kuster B., Neubauer G., Superti-Furga G. Nature 415:141-147(2002) [PubMed: 11805826] [Abstract] Cited for: INTERACTION WITH RPD3 AND SIN3.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Cti6 is an Rpd3-Sin3 histone deacetylase-associated protein required for growth under iron-limiting conditions in Saccharomyces cerevisiae." Puig S., Lau M., Thiele D.J. J. Biol. Chem. 279:30298-30306(2004) [PubMed: 15133041] [Abstract] Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-95 AND HIS-100.
[9]	"Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex." Carrozza M.J., Florens L., Swanson S.K., Shia W.-J., Anderson S., Yates J., Washburn M.P., Workman J.L. Biochim. Biophys. Acta 1731:77-87(2005) [PubMed: 16314178] [Abstract] Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, MASS SPECTROMETRY.
[10]	"Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex." Keogh M.-C., Kurdistani S.K., Morris S.A., Ahn S.H., Podolny V., Collins S.R., Schuldiner M., Chin K., Punna T., Thompson N.J., Boone C., Emili A., Weissman J.S., Hughes T.R., Strahl B.D., Grunstein M., Greenblatt J.F., Buratowski S., Krogan N.J. Cell 123:593-605(2005) [PubMed: 16286008] [Abstract] Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, MASS SPECTROMETRY.
[11]	"Recruitment of Tup1p and Cti6p regulates heme-deficient expression of Aft1p target genes." Crisp R.J., Adkins E.M., Kimmel E., Kaplan J. EMBO J. 25:512-521(2006) [PubMed: 16437160] [Abstract] Cited for: FUNCTION.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170 AND SER-216, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-364 AND SER-441, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73538 Genomic DNA. Translation: CAA97891.1. AY692957 Genomic DNA. Translation: AAT92976.1. BK006949 Genomic DNA. Translation: DAA11253.1.
PIR	S65193.
RefSeq	NP_015144.1. NM_001183995.1.
3D structure databases
ProteinModelPortal	Q08923.
SMR	Q08923. Positions 73-122.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2958N.
IntAct	Q08923. 18 interactions.
MINT	MINT-664511.
STRING	Q08923.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL181W; YPL181W; YPL181W.
GeneID	855920.
KEGG	sce:YPL181W.
NMPDR	fig|4932.3.peg.6273.
Organism-specific databases
CYGD	YPL181w.
SGD	S000006102. CTI6.
Phylogenomic databases
eggNOG	fuNOG09606.
GeneTree	EFGT00050000000943.
HOGENOM	HBG204046.
OMA	ILEFISR.
OrthoDB	EOG4ZSDBZ.
Gene expression databases
ArrayExpress	Q08923.
Genevestigator	Q08923.
GermOnline	YPL181W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF00628. PHD. 1 hit. [Graphical view]
SMART	SM00249. PHD. 1 hit. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980643.

Entry information

Entry name

CTI6_YEAST

Accession

Primary (citable) accession number: Q08923
Secondary accession number(s): D6W3I7, Q6B1X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program