Reviewed, UniProtKB/Swiss-Prot Q08911 (FDH1_YEAST)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase 1
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase 1

Gene names

Name:	FDH1
Ordered Locus Names:	YOR388C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subcellular location	Cytoplasm Ref.3.
Induction	Induced by formate. Ref.3
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADH regeneration Ref.3 Traceable author statement. Source: SGD formate catabolic process Ref.3 Inferred from genetic interaction. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.3 Inferred from direct assay. Source: SGD
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Ref.3 Inferred from genetic interaction. Source: SGD oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Formate dehydrogenase 1		PRO_0000223647
Regions
	Nucleotide binding	176 – 177	2	NAD By similarity
	Nucleotide binding	270 – 272	3	NAD By similarity
	Nucleotide binding	325 – 328	4	NAD By similarity
Sites
	Active site	272	1	By similarity
	Active site	325	1	Proton donor By similarity
	Binding site	296	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08911-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 67ECDA6F9DDC2A02	FASTA	376	41,714
	10 20 30 40 50 60 MSKGKVLLVL YEGGKHAEEQ EKLLGCIENE LGIRNFIEEQ GYELVTTIDK DPEPTSTVDR 70 80 90 100 110 120 ELKDAEIVIT TPFFPAYISR NRIAEAPNLK LCVTAGVGSD HVDLEAANER KITVTEVTGS 130 140 150 160 170 180 NVVSVAEHVM ATILVLIRNY NGGHQQAING EWDIAGVAKN EYDLEDKIIS TVGAGRIGYR 190 200 210 220 230 240 VLERLVAFNP KKLLYYDYQE LPAEAINRLN EASKLFNGRG DIVQRVEKLE DMVAQSDVVT 250 260 270 280 290 300 INCPLHKDSR GLFNKKLISH MKDGAYLVNT ARGAICVAED VAEAVKSGKL AGYGGDVWDK 310 320 330 340 350 360 QPAPKDHPWR TMDNKDHVGN AMTVHISGTS LDAQKRYAQG VKNILNSYFS KKFDYRPQDI 370 IVQNGSYATR AYGQKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Expression cassettes for formaldehyde and fluoroacetate resistance, two dominant markers in Saccharomyces cerevisiae." van den Berg M.A., Steensma H.Y. Yeast 13:551-559(1997) [PubMed: 9178506] [Abstract] Cited for: FUNCTION.
[3]	"Functional analysis of structural genes for NAD(+)-dependent formate dehydrogenase in Saccharomyces cerevisiae." Overkamp K.M., Koetter P., van der Hoek R., Schoondermark-Stolk S., Luttik M.A.H., van Dijken J.P., Pronk J.T. Yeast 19:509-520(2002) [PubMed: 11921099] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75296 Genomic DNA. Translation: CAA99720.1.
PIR	S67300.
RefSeq	NP_015033.1.
3D structure databases
SMR	Q08911. Positions 4-367.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5327N.
IntAct	Q08911. 5 interactions.
STRING	Q08911.
Genome annotation databases
Ensembl	YOR388C; YOR388C; YOR388C; Saccharomyces cerevisiae. [Genome view]
GeneID	854570.
KEGG	sce:YOR388C.
NMPDR	fig|4932.3.peg.6154.
Organism-specific databases
CYGD	YOR388c.
SGD	S000005915. FDH1.
Phylogenomic databases
eggNOG	fuNOG06903.
HOGENOM	HBG328050.
OMA	KLEDMVA.
OrthoDB	EOG9JDJQ8.
PhylomeDB	Q08911.
Enzyme and pathway databases
BRENDA	1.2.1.2. 250.
Gene expression databases
Genevestigator	Q08911.
GermOnline	YOR388C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977021.

Entry information

Entry name

FDH1_YEAST

Accession

Primary (citable) accession number: Q08911

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)