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Protein

Ferric reductase transmembrane component 3

Gene

FRE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Siderophore-iron reductase responsible for reducing extracellular iron prior to import. Catalyzes the reductive uptake of Fe3+ bound to di- and trihydroxamate siderophores. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane.1 Publication

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi316Iron (heme 1 axial ligand)By similarity1
Metal bindingi330Iron (heme 2 axial ligand)By similarity1
Metal bindingi386Iron (heme 1 axial ligand)By similarity1
Metal bindingi400Iron (heme 2 axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi479 – 485FADSequence analysis7
Nucleotide bindingi526 – 529NADPSequence analysis4
Nucleotide bindingi677 – 678NADPSequence analysis2

GO - Molecular functioni

  • ferric-chelate reductase (NADPH) activity Source: UniProtKB-EC
  • ferric-chelate reductase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • copper ion import Source: GO_Central
  • iron ion transport Source: GO_Central
  • siderophore transport Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Ion transport, Iron transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:YOR381W-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reductase transmembrane component 3 (EC:1.16.1.9)
Alternative name(s):
Ferric-chelate reductase 3
Gene namesi
Name:FRE3
Ordered Locus Names:YOR381W
ORF Names:O6754
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR381W
SGDiS000005908 FRE3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 166ExtracellularSequence analysisAdd BLAST146
Transmembranei167 – 187Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini188 – 237CytoplasmicSequence analysisAdd BLAST50
Transmembranei238 – 258Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini259 – 280ExtracellularSequence analysisAdd BLAST22
Transmembranei281 – 301Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini302 – 321CytoplasmicSequence analysisAdd BLAST20
Transmembranei322 – 341Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini342 – 353ExtracellularSequence analysisAdd BLAST12
Transmembranei354 – 374Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini375 – 376CytoplasmicSequence analysis2
Transmembranei377 – 397Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini398ExtracellularSequence analysis1
Transmembranei399 – 419Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini420 – 711CytoplasmicSequence analysisAdd BLAST292

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001013921 – 711Ferric reductase transmembrane component 3Add BLAST691

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ08905
PRIDEiQ08905

Expressioni

Inductioni

By iron deprivation.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi34762, 96 interactors
DIPiDIP-4049N
IntActiQ08905, 2 interactors
MINTiQ08905
STRINGi4932.YOR381W

Structurei

3D structure databases

ProteinModelPortaliQ08905
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 414Ferric oxidoreductaseAdd BLAST135
Domaini415 – 534FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST120

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007891
HOGENOMiHOG000000805
InParanoidiQ08905
OMAiFITLHRW
OrthoDBiEOG092C04QA

Family and domain databases

InterProiView protein in InterPro
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYWVLLCGSI LLCCLSGASA SPAKTKMYGK LPLVLTDACM GVLGEVTWEY
60 70 80 90 100
SSDDLYSSPA CTYEPALQSM LYCIYESLNE KGYSNRTFEK TFAAIKEDCA
110 120 130 140 150
YYTDNLQNMT NADFYNMLNN GTTYIIQYSE GSANLTYPIE MDAQVRENYY
160 170 180 190 200
YSYHGFYANY DIGHTYGGII CAYFVGVMIL ASILHYLSYT PFKTALFKQR
210 220 230 240 250
LVRYVRRYLT IPTIWGKHAS SFSYLKIFTG FLPTRSEGVI ILGYLVLHTV
260 270 280 290 300
FLAYGYQYDP YNLIFDSRRE QIARYVADRS GVLAFAHFPL IALFAGRNNF
310 320 330 340 350
LEFISGVKYT SFIMFHKWLG RMMFLDAVIH GAAYTSYSVF YKDWAASKEE
360 370 380 390 400
TYWQFGVAAL CIVGVMVFFS LAMFRKFFYE AFLFLHIVLG ALFFYTCWEH
410 420 430 440 450
VVELSGIEWI YAAIAIWTID RLIRIVRVSY FGFPKASLQL VGDDIIRVTV
460 470 480 490 500
KRPVRLWKAK PGQYVFVSFL HHLYFWQSHP FTVLDSIIKD GELTIILKEK
510 520 530 540 550
KGVTKLVKKY VCCNGGKASM RLAIEGPYGS SSPVNNYDNV LLLTGGTGLP
560 570 580 590 600
GPIAHAIKLG KTSAATGKQF IKLVIAVRGF NVLEAYKPEL MCLEDLNVQL
610 620 630 640 650
HIYNTMEVPA LTPNDSLEIS QQDEKADGKG VVMATTLEQS PNPVEFDGTV
660 670 680 690 700
FHHGRPNVEK LLHEVGDLNG SLAVVCCGPP VFVDEVRDQT ANLVLEKPAK
710
AIEYFEEYQS W
Length:711
Mass (Da):80,589
Last modified:November 1, 1996 - v1
Checksum:i362E18F7FA34C165
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75289 Genomic DNA Translation: CAA99713.1
BK006948 Genomic DNA Translation: DAA11139.1
PIRiS67293
RefSeqiNP_015026.1, NM_001183801.1

Genome annotation databases

EnsemblFungiiYOR381W; YOR381W; YOR381W
GeneIDi854563
KEGGisce:YOR381W

Similar proteinsi

Entry informationi

Entry nameiFRE3_YEAST
AccessioniPrimary (citable) accession number: Q08905
Secondary accession number(s): D6W373
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health