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Protein

Ferric reductase transmembrane component 3

Gene

FRE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Siderophore-iron reductase responsible for reducing extracellular iron prior to import. Catalyzes the reductive uptake of Fe3+ bound to di- and trihydroxamate siderophores. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane.1 Publication

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi316 – 3161Iron (heme 1 axial ligand)By similarity
Metal bindingi330 – 3301Iron (heme 2 axial ligand)By similarity
Metal bindingi386 – 3861Iron (heme 1 axial ligand)By similarity
Metal bindingi400 – 4001Iron (heme 2 axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi479 – 4857FADSequence analysis
Nucleotide bindingi526 – 5294NADPSequence analysis
Nucleotide bindingi677 – 6782NADPSequence analysis

GO - Molecular functioni

  • ferric-chelate reductase (NADPH) activity Source: UniProtKB-EC
  • ferric-chelate reductase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • copper ion import Source: GO_Central
  • iron ion transport Source: GO_Central
  • siderophore transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:YOR381W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reductase transmembrane component 3 (EC:1.16.1.9)
Alternative name(s):
Ferric-chelate reductase 3
Gene namesi
Name:FRE3
Ordered Locus Names:YOR381W
ORF Names:O6754
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR381W.
SGDiS000005908. FRE3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 166146ExtracellularSequence analysisAdd
BLAST
Transmembranei167 – 18721Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini188 – 23750CytoplasmicSequence analysisAdd
BLAST
Transmembranei238 – 25821Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini259 – 28022ExtracellularSequence analysisAdd
BLAST
Transmembranei281 – 30121Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini302 – 32120CytoplasmicSequence analysisAdd
BLAST
Transmembranei322 – 34120Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini342 – 35312ExtracellularSequence analysisAdd
BLAST
Transmembranei354 – 37421Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini375 – 3762CytoplasmicSequence analysis
Transmembranei377 – 39721Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini398 – 3981ExtracellularSequence analysis
Transmembranei399 – 41921Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini420 – 711292CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 711691Ferric reductase transmembrane component 3PRO_0000010139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

By iron deprivation.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi34762. 9 interactions.
DIPiDIP-4049N.
MINTiMINT-476857.

Structurei

3D structure databases

ProteinModelPortaliQ08905.
SMRiQ08905. Positions 435-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 414135Ferric oxidoreductaseAdd
BLAST
Domaini415 – 534120FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiQ08905.
KOiK00521.
OMAiKHEIDES.
OrthoDBiEOG092C04QA.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYWVLLCGSI LLCCLSGASA SPAKTKMYGK LPLVLTDACM GVLGEVTWEY
60 70 80 90 100
SSDDLYSSPA CTYEPALQSM LYCIYESLNE KGYSNRTFEK TFAAIKEDCA
110 120 130 140 150
YYTDNLQNMT NADFYNMLNN GTTYIIQYSE GSANLTYPIE MDAQVRENYY
160 170 180 190 200
YSYHGFYANY DIGHTYGGII CAYFVGVMIL ASILHYLSYT PFKTALFKQR
210 220 230 240 250
LVRYVRRYLT IPTIWGKHAS SFSYLKIFTG FLPTRSEGVI ILGYLVLHTV
260 270 280 290 300
FLAYGYQYDP YNLIFDSRRE QIARYVADRS GVLAFAHFPL IALFAGRNNF
310 320 330 340 350
LEFISGVKYT SFIMFHKWLG RMMFLDAVIH GAAYTSYSVF YKDWAASKEE
360 370 380 390 400
TYWQFGVAAL CIVGVMVFFS LAMFRKFFYE AFLFLHIVLG ALFFYTCWEH
410 420 430 440 450
VVELSGIEWI YAAIAIWTID RLIRIVRVSY FGFPKASLQL VGDDIIRVTV
460 470 480 490 500
KRPVRLWKAK PGQYVFVSFL HHLYFWQSHP FTVLDSIIKD GELTIILKEK
510 520 530 540 550
KGVTKLVKKY VCCNGGKASM RLAIEGPYGS SSPVNNYDNV LLLTGGTGLP
560 570 580 590 600
GPIAHAIKLG KTSAATGKQF IKLVIAVRGF NVLEAYKPEL MCLEDLNVQL
610 620 630 640 650
HIYNTMEVPA LTPNDSLEIS QQDEKADGKG VVMATTLEQS PNPVEFDGTV
660 670 680 690 700
FHHGRPNVEK LLHEVGDLNG SLAVVCCGPP VFVDEVRDQT ANLVLEKPAK
710
AIEYFEEYQS W
Length:711
Mass (Da):80,589
Last modified:November 1, 1996 - v1
Checksum:i362E18F7FA34C165
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75289 Genomic DNA. Translation: CAA99713.1.
BK006948 Genomic DNA. Translation: DAA11139.1.
PIRiS67293.
RefSeqiNP_015026.1. NM_001183801.1.

Genome annotation databases

EnsemblFungiiYOR381W; YOR381W; YOR381W.
GeneIDi854563.
KEGGisce:YOR381W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75289 Genomic DNA. Translation: CAA99713.1.
BK006948 Genomic DNA. Translation: DAA11139.1.
PIRiS67293.
RefSeqiNP_015026.1. NM_001183801.1.

3D structure databases

ProteinModelPortaliQ08905.
SMRiQ08905. Positions 435-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34762. 9 interactions.
DIPiDIP-4049N.
MINTiMINT-476857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR381W; YOR381W; YOR381W.
GeneIDi854563.
KEGGisce:YOR381W.

Organism-specific databases

EuPathDBiFungiDB:YOR381W.
SGDiS000005908. FRE3.

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiQ08905.
KOiK00521.
OMAiKHEIDES.
OrthoDBiEOG092C04QA.

Enzyme and pathway databases

BioCyciYEAST:YOR381W-MONOMER.

Miscellaneous databases

PROiQ08905.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRE3_YEAST
AccessioniPrimary (citable) accession number: Q08905
Secondary accession number(s): D6W373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.