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Protein

Iduronate 2-sulfatase

Gene

Ids

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.1 Publication

Catalytic activityi

Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47CalciumBy similarity1
Metal bindingi48CalciumBy similarity1
Metal bindingi86Calcium; via 3-oxoalanineBy similarity1
Metal bindingi336CalciumBy similarity1
Metal bindingi337CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2024096. HS-GAG degradation.
R-MMU-2024101. CS/DS degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Iduronate 2-sulfatase (EC:3.1.6.13)
Alternative name(s):
Alpha-L-iduronate sulfate sulfatase
Gene namesi
Name:Ids
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:96417. Ids.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000003343130 – 35By similarity6
ChainiPRO_000003343236 – 552Iduronate 2-sulfataseAdd BLAST517

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei863-oxoalanine (Cys)By similarity1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi539N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ08890.
PaxDbiQ08890.
PeptideAtlasiQ08890.
PRIDEiQ08890.

PTM databases

iPTMnetiQ08890.
PhosphoSitePlusiQ08890.

Expressioni

Tissue specificityi

Found to be expressed in alpha and beta pancreatic cells.1 Publication

Inductioni

By glucose, in a dose dependent manner.1 Publication

Gene expression databases

BgeeiENSMUSG00000035847.
CleanExiMM_IDS.
ExpressionAtlasiQ08890. baseline and differential.
GenevisibleiQ08890. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099046.

Structurei

3D structure databases

ProteinModelPortaliQ08890.
SMRiQ08890.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00730000110906.
HOVERGENiHBG006120.
InParanoidiQ08890.
KOiK01136.
OMAiDDYPLSW.
OrthoDBiEOG091G0B5B.
TreeFamiTF323156.

Family and domain databases

CDDicd16030. iduronate-2-sulfatase. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core_sf.
IPR035874. IDS.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
PfamiView protein in Pfam
PF00884. Sulfatase. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiView protein in PROSITE
PS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPPPPPIW RQLSFSLLLG SFCIALESAA QGNSATDALN ILLIIVDDLR
60 70 80 90 100
PSLGCYGDKL VRSPNIDQLA SHSVLFQNAF AQQAVCAPSR VSFLTGRRPD
110 120 130 140 150
TTRLYDFNSY WRVHSGNFST IPQYFKENGY VTMSVGKVFH PGISSNHSDD
160 170 180 190 200
YPYSWSFPPY HPSSEKYENT KTCKGQDGKL HANLLCPVDV ADVPEGTLPD
210 220 230 240 250
KQSTEEAIRL LEKMKTSASP FFLAVGYHKP HIPFRYPKEF QKLYPLENIT
260 270 280 290 300
LAPDPHVPDS LPPVAYNPWM DIREREDVQA LNISVPYGPI PEDFQRKIRQ
310 320 330 340 350
SYFASVSYLD TQVGHVLSAL DDLRLAHNTI IAFTSDHGWA LGEHGEWAKY
360 370 380 390 400
SNFDVATRVP LMLYVPGRTA PLPAAGQKLF PYRDPFDPAS DWMDAGRHTE
410 420 430 440 450
DLVELVSLFP TLAGLAGLPV PPRCPIPSFH VELCREGQNL QKHLQLHDLE
460 470 480 490 500
EEPDLFGNPR ELIAYSQYPR PADFPQWNSD KPSLNDIKVM GYSIRTVDYR
510 520 530 540 550
YTVWVGFDPS EFLANFSDIH AGELYFVDSD PLQDHNVYND SQHGGLLHSL

RP
Length:552
Mass (Da):62,186
Last modified:July 27, 2011 - v3
Checksum:i3E3D819A823AFDE5
GO

Sequence cautioni

The sequence AAA37880 differs from that shown. Reason: Frameshift at positions 420, 458, 488, 493 and 497.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41I → V in BAE38612 (PubMed:16141072).Curated1
Sequence conflicti218A → G in AAA37880 (PubMed:8325651).Curated1
Sequence conflicti483S → T in AAA37880 (PubMed:8325651).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK166178 mRNA. Translation: BAE38612.1.
BX294168 Genomic DNA. Translation: CAM20128.1.
L07921 mRNA. Translation: AAA37880.1. Frameshift.
BN000750 mRNA. Translation: CAI84996.1.
CCDSiCCDS40996.1.
PIRiA47153.
RefSeqiNP_034628.2. NM_010498.3.
UniGeneiMm.233083.

Genome annotation databases

EnsembliENSMUST00000101509; ENSMUSP00000099046; ENSMUSG00000035847.
GeneIDi15931.
KEGGimmu:15931.
UCSCiuc012hjl.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiIDS_MOUSE
AccessioniPrimary (citable) accession number: Q08890
Secondary accession number(s): Q32KI7, Q3TM30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families