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Q08890

- IDS_MOUSE

UniProt

Q08890 - IDS_MOUSE

Protein

Iduronate 2-sulfatase

Gene

Ids

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.1 Publication

    Catalytic activityi

    Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471CalciumBy similarity
    Metal bindingi48 – 481CalciumBy similarity
    Metal bindingi86 – 861Calcium; via 3-oxoalanineBy similarity
    Metal bindingi336 – 3361CalciumBy similarity
    Metal bindingi337 – 3371CalciumBy similarity

    GO - Molecular functioni

    1. iduronate-2-sulfatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. sulfuric ester hydrolase activity Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198967. HS-GAG degradation.
    REACT_198981. CS/DS degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iduronate 2-sulfatase (EC:3.1.6.13)
    Alternative name(s):
    Alpha-L-iduronate sulfate sulfatase
    Gene namesi
    Name:Ids
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:96417. Ids.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Propeptidei30 – 356By similarityPRO_0000033431
    Chaini36 – 552517Iduronate 2-sulfatasePRO_0000033432Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei86 – 8613-oxoalanine (Cys)By similarity
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

    Keywords - PTMi

    Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ08890.
    PRIDEiQ08890.

    PTM databases

    PhosphoSiteiQ08890.

    Expressioni

    Tissue specificityi

    Found to be expressed in alpha and beta pancreatic cells.1 Publication

    Inductioni

    By glucose, in a dose dependent manner.1 Publication

    Gene expression databases

    ArrayExpressiQ08890.
    BgeeiQ08890.
    CleanExiMM_IDS.
    GenevestigatoriQ08890.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08890.
    SMRiQ08890. Positions 40-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sulfatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3119.
    GeneTreeiENSGT00730000110906.
    HOVERGENiHBG006120.
    InParanoidiQ32KI7.
    KOiK01136.
    OMAiDHNMYND.
    OrthoDBiEOG7PK8ZR.
    TreeFamiTF323156.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR000917. Sulfatase.
    IPR024607. Sulfatase_CS.
    [Graphical view]
    PfamiPF00884. Sulfatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 2 hits.
    PROSITEiPS00523. SULFATASE_1. 1 hit.
    PS00149. SULFATASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08890-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPPPPPPIW RQLSFSLLLG SFCIALESAA QGNSATDALN ILLIIVDDLR    50
    PSLGCYGDKL VRSPNIDQLA SHSVLFQNAF AQQAVCAPSR VSFLTGRRPD 100
    TTRLYDFNSY WRVHSGNFST IPQYFKENGY VTMSVGKVFH PGISSNHSDD 150
    YPYSWSFPPY HPSSEKYENT KTCKGQDGKL HANLLCPVDV ADVPEGTLPD 200
    KQSTEEAIRL LEKMKTSASP FFLAVGYHKP HIPFRYPKEF QKLYPLENIT 250
    LAPDPHVPDS LPPVAYNPWM DIREREDVQA LNISVPYGPI PEDFQRKIRQ 300
    SYFASVSYLD TQVGHVLSAL DDLRLAHNTI IAFTSDHGWA LGEHGEWAKY 350
    SNFDVATRVP LMLYVPGRTA PLPAAGQKLF PYRDPFDPAS DWMDAGRHTE 400
    DLVELVSLFP TLAGLAGLPV PPRCPIPSFH VELCREGQNL QKHLQLHDLE 450
    EEPDLFGNPR ELIAYSQYPR PADFPQWNSD KPSLNDIKVM GYSIRTVDYR 500
    YTVWVGFDPS EFLANFSDIH AGELYFVDSD PLQDHNVYND SQHGGLLHSL 550
    RP 552
    Length:552
    Mass (Da):62,186
    Last modified:July 27, 2011 - v3
    Checksum:i3E3D819A823AFDE5
    GO

    Sequence cautioni

    The sequence AAA37880.1 differs from that shown. Reason: Frameshift at positions 420, 458, 488, 493 and 497.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti41 – 411I → V in BAE38612. (PubMed:16141072)Curated
    Sequence conflicti218 – 2181A → G in AAA37880. (PubMed:8325651)Curated
    Sequence conflicti483 – 4831S → T in AAA37880. (PubMed:8325651)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK166178 mRNA. Translation: BAE38612.1.
    BX294168 Genomic DNA. Translation: CAM20128.1.
    L07921 mRNA. Translation: AAA37880.1. Frameshift.
    BN000750 mRNA. Translation: CAI84996.1.
    CCDSiCCDS40996.1.
    PIRiA47153.
    RefSeqiNP_034628.2. NM_010498.3.
    UniGeneiMm.233083.

    Genome annotation databases

    EnsembliENSMUST00000101509; ENSMUSP00000099046; ENSMUSG00000035847.
    GeneIDi15931.
    KEGGimmu:15931.
    UCSCiuc012hjl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK166178 mRNA. Translation: BAE38612.1 .
    BX294168 Genomic DNA. Translation: CAM20128.1 .
    L07921 mRNA. Translation: AAA37880.1 . Frameshift.
    BN000750 mRNA. Translation: CAI84996.1 .
    CCDSi CCDS40996.1.
    PIRi A47153.
    RefSeqi NP_034628.2. NM_010498.3.
    UniGenei Mm.233083.

    3D structure databases

    ProteinModelPortali Q08890.
    SMRi Q08890. Positions 40-413.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q08890.

    Proteomic databases

    PaxDbi Q08890.
    PRIDEi Q08890.

    Protocols and materials databases

    DNASUi 15931.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000101509 ; ENSMUSP00000099046 ; ENSMUSG00000035847 .
    GeneIDi 15931.
    KEGGi mmu:15931.
    UCSCi uc012hjl.1. mouse.

    Organism-specific databases

    CTDi 3423.
    MGIi MGI:96417. Ids.

    Phylogenomic databases

    eggNOGi COG3119.
    GeneTreei ENSGT00730000110906.
    HOVERGENi HBG006120.
    InParanoidi Q32KI7.
    KOi K01136.
    OMAi DHNMYND.
    OrthoDBi EOG7PK8ZR.
    TreeFami TF323156.

    Enzyme and pathway databases

    Reactomei REACT_198967. HS-GAG degradation.
    REACT_198981. CS/DS degradation.

    Miscellaneous databases

    ChiTaRSi IDS. mouse.
    NextBioi 288652.
    PROi Q08890.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q08890.
    Bgeei Q08890.
    CleanExi MM_IDS.
    Genevestigatori Q08890.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR000917. Sulfatase.
    IPR024607. Sulfatase_CS.
    [Graphical view ]
    Pfami PF00884. Sulfatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 2 hits.
    PROSITEi PS00523. SULFATASE_1. 1 hit.
    PS00149. SULFATASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Cloning and characterization of the cDNA for the murine iduronate sulfatase gene."
      Daniele A., Faust C.J., Herman G.E., di Natale P., Ballabio A.
      Genomics 16:755-757(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-552.
      Tissue: Thymus.
    4. "Identification of iduronate-2-sulfatase in mouse pancreatic islets."
      Coronado-Pons I., Novials A., Casas S., Clark A., Gomis R.
      Am. J. Physiol. 287:E983-E990(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    5. "Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship."
      Sardiello M., Annunziata I., Roma G., Ballabio A.
      Hum. Mol. Genet. 14:3203-3217(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiIDS_MOUSE
    AccessioniPrimary (citable) accession number: Q08890
    Secondary accession number(s): Q32KI7, Q3TM30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3