Q08887 (NDD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear division defective protein 1

Gene names

Name:	NDD1
Ordered Locus Names:	YOR372C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription activator involved in G2/M transcription through its association with FKH2. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Ref.11 Ref.12
Subunit structure	Forms an activator complex with FKH2.
Subcellular location	Cytoplasm. Nucleus Ref.3 Ref.9.
Induction	During S phase of the cell cycle. Expression is cell cycle regulated by HCM1. Ref.3 Ref.12
Post-translational modification	Phosphorylation of Thr-319 by CDC28 is required for the interaction with FKH2 and recruitment to promoters.
Miscellaneous	Present with 779 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription involved in G2/M-phase of mitotic cell cycle Inferred from mutant phenotype Ref.3. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular_function	transcription coactivator activity Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Nuclear division defective protein 1		PRO_0000268686
Regions
	Compositional bias	96 – 113	18	Gln-rich
	Compositional bias	493 – 546	54	Ser-rich
Amino acid modifications
	Modified residue	254	1	Phosphoserine Ref.13
	Modified residue	319	1	Phosphothreonine; by CDC28 Ref.8 Ref.13
	Modified residue	527	1	Phosphoserine Ref.13
	Modified residue	530	1	Phosphoserine Ref.13
Experimental info
	Mutagenesis	319	1	T → A: Growth delay. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q08887 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1CF99115F2B1F23D	FASTA	554	60,523
	10 20 30 40 50 60 MDRDISYQQN YTSTGATATS SRQPSTDNNA DTNFLKVMSE FKYNFNSPLP TTTQFPTPYS 70 80 90 100 110 120 SNQYQQTQDH FANTDAHNSS SNESSLVENS ILPHHQQIQQ QQQQQQQQQQ QQQALGSLVP 130 140 150 160 170 180 PAVTRTDTSE TLDDINVQPS SVLQFGNSLP SEFLVASPEQ FKEFLLDSPS TNFNFFHKTP 190 200 210 220 230 240 AKTPLRFVTD SNGAQQSTTE NPGQQQNVFS NVDLNNLLKS NGKTPSSSCT GAFSRTPLSK 250 260 270 280 290 300 IDMNLMFNQP LPTSPSKRFS SLSLTPYGRK ILNDVGTPYA KALISSNSAL VDFQKARKDI 310 320 330 340 350 360 TTNATSIGLE NANNILQRTP LRSNNKKLFI KTPQDTINST STLTKDNENK QDIYGSSPTT 370 380 390 400 410 420 IQLNSSITKS ISKLDNSRIP LLASRSDNIL DSNVDDQLFD LGLTRLPLSP TPNCNSLHST 430 440 450 460 470 480 TTGTSALQIP ELPKMGSFRS DTGINPISSS NTVSFKSKSG NNNSKGRIKK NGKKPSKFQI 490 500 510 520 530 540 IVANIDQFNQ DTSSSSLSSS LNASSSAGNS NSNVTKKRAS KLKRSQSLLS DSGSKSQARK 550 SCNSKSNGNL FNSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"NDD1, a high-dosage suppressor of cdc28-1N, is essential for expression of a subset of late-S-phase-specific genes in Saccharomyces cerevisiae." Loy C.J., Lydall D., Surana U. Mol. Cell. Biol. 19:3312-3327(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[4]	"Forkhead-like transcription factors recruit Ndd1 to the chromatin of G2/M-specific promoters." Koranda M., Schleiffer A., Endler L., Ammerer G. Nature 406:94-98(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Serial regulation of transcriptional regulators in the yeast cell cycle." Simon I., Barnett J., Hannett N., Harbison C.T., Rinaldi N.J., Volkert T.L., Wyrick J.J., Zeitlinger J., Gifford D.K., Jaakkola T.S., Young R.A. Cell 106:697-708(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer." Sun K., Coic E., Zhou Z., Durrens P., Haber J.E. Genes Dev. 16:2085-2096(2002) [PubMed] [Europe PMC] [Abstract] Cited for: RECRUITMENT TO PROMOTER.
[7]	"Cell cycle-regulated transcription through the FHA domain of Fkh2p and the coactivator Ndd1p." Darieva Z., Pic-Taylor A., Boros J., Spanos A., Geymonat M., Reece R.J., Sedgwick S.G., Sharrocks A.D., Morgan B.A. Curr. Biol. 13:1740-1745(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION.
[8]	"Recruitment of Thr 319-phosphorylated Ndd1p to the FHA domain of Fkh2p requires Clb kinase activity: a mechanism for CLB cluster gene activation." Reynolds D., Shi B.J., McLean C., Katsis F., Kemp B., Dalton S. Genes Dev. 17:1789-1802(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FKH2, PHOSPHORYLATION AT THR-319, MUTAGENESIS OF THR-319.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Inferring yeast cell cycle regulators and interactions using transcription factor activities." Yang Y.-L., Suen J., Brynildsen M.P., Galbraith S.J., Liao J.C. BMC Genomics 6:90-90(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"The Forkhead transcription factor Hcm1 regulates chromosome segregation genes and fills the S-phase gap in the transcriptional circuitry of the cell cycle." Pramila T., Wu W., Miles S., Noble W.S., Breeden L.L. Genes Dev. 20:2266-2278(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254; THR-319; SER-527 AND SER-530, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75280 Genomic DNA. Translation: CAA99703.1. BK006948 Genomic DNA. Translation: DAA11131.1.
PIR	S67284.
RefSeq	NP_015017.1. NM_001183792.1.
3D structure databases
ProteinModelPortal	Q08887.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1806N.
IntAct	Q08887. 3 interactions.
MINT	MINT-393428.
STRING	4932.YOR372C.
Proteomic databases
PaxDb	Q08887.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR372C; YOR372C; YOR372C.
GeneID	854554.
KEGG	sce:YOR372C.
Organism-specific databases
CYGD	YOR372c.
SGD	S000005899. NDD1.
Phylogenomic databases
eggNOG	NOG39287.
HOGENOM	HOG000066011.
OMA	ELPKMGS.
OrthoDB	EOG4HB1VW.
Gene expression databases
Genevestigator	Q08887.
GermOnline	YOR372C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	976979.

Entry information

Entry name

NDD1_YEAST

Accession

Primary (citable) accession number: Q08887
Secondary accession number(s): D6W365

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program