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Protein

DNA polymerase III subunit epsilon

Gene

dnaQ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 divalent metal cations. Magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Divalent metal cation 1; catalyticBy similarity1
Metal bindingi11Divalent metal cation 2; catalyticBy similarity1
Binding sitei11SubstrateBy similarity1
Metal bindingi13Divalent metal cation 1; catalyticBy similarity1
Binding sitei13SubstrateBy similarity1
Binding sitei60SubstrateBy similarity1
Binding sitei65SubstrateBy similarity1
Active sitei161Proton acceptorBy similarity1
Metal bindingi166Divalent metal cation 1; catalyticBy similarity1
Binding sitei166SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-257-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit epsilon (EC:2.7.7.7)
Gene namesi
Name:dnaQ
Synonyms:mutD
Ordered Locus Names:BUsg_240
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054811 – 233DNA polymerase III subunit epsilonAdd BLAST233

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] (By similarity).By similarity

Protein-protein interaction databases

STRINGi198804.BUsg240

Structurei

3D structure databases

ProteinModelPortaliQ08880
SMRiQ08880
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4107RZZ Bacteria
COG0847 LUCA
KOiK02342
OMAiFHVYLNP
OrthoDBiPOG091H0294

Family and domain databases

CDDicd06131 DNA_pol_III_epsilon_Ecoli_like, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR006054 DnaQ
IPR006309 DnaQ_proteo
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00573 dnaq, 1 hit
TIGR01406 dnaQ_proteo, 1 hit

Sequencei

Sequence statusi: Complete.

Q08880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNTQRIIVL DTETTGMNSV GPPYLNHRII EIGAIEIINR RFTGKKFHTY
60 70 80 90 100
IKPNRLIESD ASKIHGITDD FLSDKPSFKD IAKDFFNYIK NSELIIHNAS
110 120 130 140 150
FDVGFINQEF SMLTKKIQDI SNFCNIIDTL KIARKLFPGK KNTLDALCMR
160 170 180 190 200
YKIKNSHRVL HGAILDAFLL GKLYLLMTSG QESIIFNKNI QNERNFRYIK
210 220 230
KSITKKHRFL KIIKANKTEL KLHNEYLKFL KEK
Length:233
Mass (Da):27,170
Last modified:November 1, 1997 - v2
Checksum:iF722E4C65DB1E583
GO

Sequence cautioni

The sequence AAM67799 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77464 Genomic DNA Translation: AAC44791.1
AE013218 Genomic DNA Translation: AAM67799.1 Different initiation.
L18927 Genomic DNA Translation: AAA17435.1
PIRiI40594
RefSeqiWP_044006048.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67799; AAM67799; BUsg_240
KEGGibas:BUsg_240

Similar proteinsi

Entry informationi

Entry nameiDPO3E_BUCAP
AccessioniPrimary (citable) accession number: Q08880
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health