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Q08880 (DPO3E_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit epsilon

EC=2.7.7.7
Gene names
Name:dnaQ
Synonyms:mutD
Ordered Locus Names:BUsg_240
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactor

Binds 2 divalent metal cations. Magnesium or manganese By similarity.

Subunit structure

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] By similarity.

Sequence caution

The sequence AAM67799.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233DNA polymerase III subunit epsilon
PRO_0000105481

Sites

Active site1611Proton acceptor By similarity
Metal binding111Divalent metal cation 1; catalytic By similarity
Metal binding111Divalent metal cation 2; catalytic By similarity
Metal binding131Divalent metal cation 1; catalytic By similarity
Metal binding1661Divalent metal cation 1; catalytic By similarity
Binding site111Substrate By similarity
Binding site131Substrate By similarity
Binding site601Substrate By similarity
Binding site651Substrate By similarity
Binding site1661Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08880 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: F722E4C65DB1E583

FASTA23327,170
        10         20         30         40         50         60 
MNNTQRIIVL DTETTGMNSV GPPYLNHRII EIGAIEIINR RFTGKKFHTY IKPNRLIESD 

        70         80         90        100        110        120 
ASKIHGITDD FLSDKPSFKD IAKDFFNYIK NSELIIHNAS FDVGFINQEF SMLTKKIQDI 

       130        140        150        160        170        180 
SNFCNIIDTL KIARKLFPGK KNTLDALCMR YKIKNSHRVL HGAILDAFLL GKLYLLMTSG 

       190        200        210        220        230 
QESIIFNKNI QNERNFRYIK KSITKKHRFL KIIKANKTEL KLHNEYLKFL KEK 

« Hide

References

« Hide 'large scale' references
[1]"Evolution of dnaQ, the gene encoding the editing 3' to 5' exonuclease subunit of DNA polymerase III holoenzyme in Gram-negative bacteria."
Huang Y., Braithwaite D.K., Ito J.
FEBS Lett. 400:94-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.
[3]"Buchnera aphidicola (a prokaryotic endosymbiont of aphids) contains a putative 16S rRNA operon unlinked to the 23S rRNA-encoding gene: sequence determination, and promoter and terminator analysis."
Munson M.A., Baumann L., Baumann P.
Gene 137:171-178(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77464 Genomic DNA. Translation: AAC44791.1.
AE013218 Genomic DNA. Translation: AAM67799.1. Different initiation.
L18927 Genomic DNA. Translation: AAA17435.1.
PIRI40594.
RefSeqNP_660588.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ08880.
SMRQ08880. Positions 6-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67799; AAM67799; BUsg_240.
GeneID1005441.
KEGGbas:BUsg240.
PATRIC21247281. VBIBucAph100086_0251.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0847.
KOK02342.
OMAFHVYLNP.
OrthoDBEOG696BTR.
ProtClustDBCLSK315823.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-253-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
InterProIPR006054. DnaQ.
IPR006309. DnaQ_proteo.
IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00573. dnaq. 1 hit.
TIGR01406. dnaQ_proteo. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3E_BUCAP
AccessionPrimary (citable) accession number: Q08880
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names