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Q08880

- DPO3E_BUCAP

UniProt

Q08880 - DPO3E_BUCAP

Protein

DNA polymerase III subunit epsilon

Gene

dnaQ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    Cofactori

    Binds 2 divalent metal cations. Magnesium or manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111Divalent metal cation 1; catalyticBy similarity
    Metal bindingi11 – 111Divalent metal cation 2; catalyticBy similarity
    Binding sitei11 – 111SubstrateBy similarity
    Metal bindingi13 – 131Divalent metal cation 1; catalyticBy similarity
    Binding sitei13 – 131SubstrateBy similarity
    Binding sitei60 – 601SubstrateBy similarity
    Binding sitei65 – 651SubstrateBy similarity
    Active sitei161 – 1611Proton acceptorBy similarity
    Metal bindingi166 – 1661Divalent metal cation 1; catalyticBy similarity
    Binding sitei166 – 1661SubstrateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. exonuclease activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-253-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase III subunit epsilon (EC:2.7.7.7)
    Gene namesi
    Name:dnaQ
    Synonyms:mutD
    Ordered Locus Names:BUsg_240
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 233233DNA polymerase III subunit epsilonPRO_0000105481Add
    BLAST

    Interactioni

    Subunit structurei

    The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] By similarity.By similarity

    Protein-protein interaction databases

    STRINGi198804.BUsg240.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08880.
    SMRiQ08880. Positions 6-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG0847.
    KOiK02342.
    OMAiGFMDHEF.
    OrthoDBiEOG696BTR.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    InterProiIPR006054. DnaQ.
    IPR006309. DnaQ_proteo.
    IPR006055. Exonuclease.
    IPR013520. Exonuclease_RNaseT/DNA_pol3.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00929. RNase_T. 1 hit.
    [Graphical view]
    SMARTiSM00479. EXOIII. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00573. dnaq. 1 hit.
    TIGR01406. dnaQ_proteo. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08880-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNTQRIIVL DTETTGMNSV GPPYLNHRII EIGAIEIINR RFTGKKFHTY    50
    IKPNRLIESD ASKIHGITDD FLSDKPSFKD IAKDFFNYIK NSELIIHNAS 100
    FDVGFINQEF SMLTKKIQDI SNFCNIIDTL KIARKLFPGK KNTLDALCMR 150
    YKIKNSHRVL HGAILDAFLL GKLYLLMTSG QESIIFNKNI QNERNFRYIK 200
    KSITKKHRFL KIIKANKTEL KLHNEYLKFL KEK 233
    Length:233
    Mass (Da):27,170
    Last modified:November 1, 1997 - v2
    Checksum:iF722E4C65DB1E583
    GO

    Sequence cautioni

    The sequence AAM67799.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77464 Genomic DNA. Translation: AAC44791.1.
    AE013218 Genomic DNA. Translation: AAM67799.1. Different initiation.
    L18927 Genomic DNA. Translation: AAA17435.1.
    PIRiI40594.
    RefSeqiNP_660588.1. NC_004061.1.
    WP_011053766.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67799; AAM67799; BUsg_240.
    GeneIDi1005441.
    KEGGibas:BUsg240.
    PATRICi21247281. VBIBucAph100086_0251.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77464 Genomic DNA. Translation: AAC44791.1 .
    AE013218 Genomic DNA. Translation: AAM67799.1 . Different initiation.
    L18927 Genomic DNA. Translation: AAA17435.1 .
    PIRi I40594.
    RefSeqi NP_660588.1. NC_004061.1.
    WP_011053766.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q08880.
    SMRi Q08880. Positions 6-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67799 ; AAM67799 ; BUsg_240 .
    GeneIDi 1005441.
    KEGGi bas:BUsg240.
    PATRICi 21247281. VBIBucAph100086_0251.

    Phylogenomic databases

    eggNOGi COG0847.
    KOi K02342.
    OMAi GFMDHEF.
    OrthoDBi EOG696BTR.

    Enzyme and pathway databases

    BioCyci BAPH198804:GHMG-253-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    InterProi IPR006054. DnaQ.
    IPR006309. DnaQ_proteo.
    IPR006055. Exonuclease.
    IPR013520. Exonuclease_RNaseT/DNA_pol3.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00929. RNase_T. 1 hit.
    [Graphical view ]
    SMARTi SM00479. EXOIII. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00573. dnaq. 1 hit.
    TIGR01406. dnaQ_proteo. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of dnaQ, the gene encoding the editing 3' to 5' exonuclease subunit of DNA polymerase III holoenzyme in Gram-negative bacteria."
      Huang Y., Braithwaite D.K., Ito J.
      FEBS Lett. 400:94-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.
    3. "Buchnera aphidicola (a prokaryotic endosymbiont of aphids) contains a putative 16S rRNA operon unlinked to the 23S rRNA-encoding gene: sequence determination, and promoter and terminator analysis."
      Munson M.A., Baumann L., Baumann P.
      Gene 137:171-178(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179.

    Entry informationi

    Entry nameiDPO3E_BUCAP
    AccessioniPrimary (citable) accession number: Q08880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

    External Data

    Dasty 3