Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dynamin-3

Gene

Dnm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469GTPBy similarity
Nucleotide bindingi205 – 2117GTPBy similarity
Nucleotide bindingi236 – 2394GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW
  • nitric-oxide synthase binding Source: RGD
  • type 1 metabotropic glutamate receptor binding Source: RGD
  • type 5 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  • endocytosis Source: UniProtKB
  • filopodium assembly Source: UniProtKB
  • negative regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of filopodium assembly Source: RGD
  • regulation of dendritic spine morphogenesis Source: RGD
  • sperm ejaculation Source: UniProtKB
  • synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.5. 5301.
ReactomeiR-RNO-177504. Retrograde neurotrophin signalling.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-437239. Recycling pathway of L1.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-3 (EC:3.6.5.5)
Alternative name(s):
Dynamin, testicular
T-dynamin
Gene namesi
Name:Dnm3
Synonyms:Dyn3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi727949. Dnm3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmcytoskeleton 1 Publication

  • Note: Microtubule-associated. Isoform-specific localization.1 Publication
Isoform 2 :
Isoform 8 :

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: RGD
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB
  • dendritic spine Source: UniProtKB
  • dendritic spine head Source: RGD
  • extracellular exosome Source: Ensembl
  • Golgi apparatus Source: RGD
  • microtubule Source: UniProtKB-KW
  • mitochondrion Source: Ensembl
  • perinuclear region of cytoplasm Source: UniProtKB
  • photoreceptor inner segment Source: Ensembl
  • plasma membrane Source: Reactome
  • postsynaptic density Source: UniProtKB
  • synapse Source: RGD
  • synaptic cleft Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Dynamin-3PRO_0000206574Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801PhosphotyrosineBy similarity
Modified residuei125 – 1251Nitrated tyrosine; alternateBy similarity
Modified residuei125 – 1251Phosphotyrosine; alternateBy similarity
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei347 – 3471PhosphoserineBy similarity
Modified residuei769 – 7691PhosphoserineCombined sources1 Publication
Modified residuei773 – 7731PhosphoserineCombined sources1 Publication
Modified residuei853 – 8531Phosphoserine1 Publication

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

PaxDbiQ08877.
PRIDEiQ08877.

PTM databases

iPTMnetiQ08877.
PhosphoSiteiQ08877.

Expressioni

Tissue specificityi

Isoform-specific expression in germ-cell-depleted testis (Sertoli cells), brain (peripheral sensory neurons), lung and heart.2 Publications

Developmental stagei

Up-regulated expression throughout development.1 Publication

Gene expression databases

GenevisibleiQ08877. RN.

Interactioni

GO - Molecular functioni

  • nitric-oxide synthase binding Source: RGD
  • type 1 metabotropic glutamate receptor binding Source: RGD
  • type 5 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

BioGridi251309. 2 interactions.
IntActiQ08877. 2 interactions.
STRINGi10116.ENSRNOP00000060160.

Structurei

3D structure databases

ProteinModelPortaliQ08877.
SMRiQ08877. Positions 6-304, 525-635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 294267Dynamin-type GAdd
BLAST
Domaini525 – 631107PHPROSITE-ProRule annotationAdd
BLAST
Domaini659 – 75092GEDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi753 – 862110Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
GeneTreeiENSGT00760000119213.
HOGENOMiHOG000161069.
HOVERGENiHBG107833.
InParanoidiQ08877.
KOiK01528.
PhylomeDBiQ08877.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (13)i

Sequence statusi: Complete.

This entry describes 13 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08877-1) [UniParc]FASTAAdd to basket

Also known as: bab, DynIIIbb

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNREMEELI PLVNRLQDAF SALGQSCLLE LPQIAVVGGQ SAGKSSVLEN
60 70 80 90 100
FVGRDFLPRG SGIVTRRPLV LQLVTSKAEY AEFLHCKGKK FTDFDEVRHE
110 120 130 140 150
IEAETDRVTG MNKGISSVPI NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP
160 170 180 190 200
DIEYQIRDMI MQFITRENCL ILAVTPANTD LANSDALKLA KEVDPQGLRT
210 220 230 240 250
IGVITKLDLM DEGTDARDVL ENKLLPLRRG YVGVVNRSQK DIDGKKDIKA
260 270 280 290 300
AMLAERKFFL SHPAYRHIAD RMGTPHLQKV LNQQLTNHIR DTLPNFRNKL
310 320 330 340 350
QGQLLSIEHE VEAFKNFKPE DPTRKTKALL QMVQQFAVDF EKRIEGSGDQ
360 370 380 390 400
VDTLELSGGA KINRIFHERF PFEIVKMEFN EKELRREISY AIKNIHGIRT
410 420 430 440 450
GLFTPDMAFE AIVKKQIVKL KGPSLKSVDL VMQELINTVK KCTKRLANFP
460 470 480 490 500
RLCEETERIV ANHIREREGK TKDQVLLLID IQVSYINTNH EDFIGFANAQ
510 520 530 540 550
QRSSQVHKKS TIGNQGTNLP PSRQIVIRKG WLTVSNIGIM KGGSKGYWFV
560 570 580 590 600
LTAESLSWYK DDEEKEKKYM LPLDNLKVRD VEKGFMSSKH VFALFNTEQR
610 620 630 640 650
NVYKDYRSLE LACDSQEDVD SWKASLLRAG VYPDKSFTEN DENGQAENFS
660 670 680 690 700
MDPQLERQVE TIRNLVDSYM SIINKCIRDL IPKTIMHLMI NNVKDFINSE
710 720 730 740 750
LLAQLYSSED QNTLMEESVE QAQRRDEMLR MYQALKEALA IIGDINTVTV
760 770 780 790 800
STPAPPPVDD SWLQHSRRSP PPSPTTQRRL TLSAPLPRPA SSRGPAPAIP
810 820 830 840 850
SPGPHSGAPP VPFRPGPLPP FPNSSDSYGA PPQVPSRPTR APPSVPSRRP
860
PPSPTRPTII RPLESSLLD
Note: Expressed in lung, brain, heart.
Length:869
Mass (Da):97,914
Last modified:June 10, 2008 - v2
Checksum:i00B41E41E5425BAD
GO
Isoform 2 (identifier: Q08877-2) [UniParc]FASTAAdd to basket

Also known as: baa, DynIIIba

The sequence of this isoform differs from the canonical sequence as follows:
     847-869: SRRPPPSPTRPTIIRPLESSLLD → RFGAVKEEAVEP

Note: Expressed in lung, brain, heart, testis. Localized to vesicular-like punctate spots, neither at the plasma membrane nor the Golgi area.
Show »
Length:858
Mass (Da):96,660
Checksum:iA54B96E1388DD0CA
GO
Isoform 3 (identifier: Q08877-3) [UniParc]FASTAAdd to basket

Also known as: bbb

The sequence of this isoform differs from the canonical sequence as follows:
     636-640: SFTEN → SFGSNKTEM

Note: Expressed in lung, brain, heart, testis.
Show »
Length:873
Mass (Da):98,318
Checksum:iB57DCEFF9E6696DA
GO
Isoform 4 (identifier: Q08877-4) [UniParc]FASTAAdd to basket

Also known as: bba

The sequence of this isoform differs from the canonical sequence as follows:
     636-640: SFTEN → SFGSNKTEM
     847-869: SRRPPPSPTRPTIIRPLESSLLD → RFGAVKEEAVEP

Note: Expressed in lung, brain, heart.
Show »
Length:862
Mass (Da):97,063
Checksum:i3AED72E98B90062E
GO
Isoform 5 (identifier: Q08877-5) [UniParc]FASTAAdd to basket

Also known as: bcb

The sequence of this isoform differs from the canonical sequence as follows:
     636-640: SFTEN → DQAENEDGAQENTF

Note: Expressed in lung.
Show »
Length:878
Mass (Da):98,885
Checksum:iFD1BCCBFB42A2FD8
GO
Isoform 6 (identifier: Q08877-6) [UniParc]FASTAAdd to basket

Also known as: bca

The sequence of this isoform differs from the canonical sequence as follows:
     636-640: SFTEN → DQAENEDGAQENTF
     847-869: SRRPPPSPTRPTIIRPLESSLLD → RFGAVKEEAVEP

Note: Expressed in lung.
Show »
Length:867
Mass (Da):97,631
Checksum:iB1B5E94CDD159795
GO
Isoform 7 (identifier: Q08877-7) [UniParc]FASTAAdd to basket

Also known as: aab, DynIIIab

The sequence of this isoform differs from the canonical sequence as follows:
     516-525: Missing.

Note: Expressed in lung, brain, heart, testis.
Show »
Length:859
Mass (Da):96,850
Checksum:i43526CBCC8DCA610
GO
Isoform 8 (identifier: Q08877-9) [UniParc]FASTAAdd to basket

Also known as: aaa, DynIIIaa

The sequence of this isoform differs from the canonical sequence as follows:
     516-525: Missing.
     847-869: SRRPPPSPTRPTIIRPLESSLLD → RFGAVKEEAVEP

Note: Expressed in lung, brain, heart, testis. Diffuse cytoplasmic distribution and some modest association with the Golgi apparatus.
Show »
Length:848
Mass (Da):95,596
Checksum:i802E365FCFC685F6
GO
Isoform 9 (identifier: Q08877-10) [UniParc]FASTAAdd to basket

Also known as: abb

The sequence of this isoform differs from the canonical sequence as follows:
     516-525: Missing.
     636-640: SFTEN → SFGSNKTEM

Note: Expressed in lung, brain, heart.
Show »
Length:863
Mass (Da):97,254
Checksum:i89467DF02D80E427
GO
Isoform 10 (identifier: Q08877-11) [UniParc]FASTAAdd to basket

Also known as: aba

The sequence of this isoform differs from the canonical sequence as follows:
     516-525: Missing.
     636-640: SFTEN → SFGSNKTEM
     847-869: SRRPPPSPTRPTIIRPLESSLLD → RFGAVKEEAVEP

Note: Expressed in lung, brain, heart.
Show »
Length:852
Mass (Da):95,999
Checksum:iA79E52BFEEF5A690
GO
Isoform 11 (identifier: Q08877-12) [UniParc]FASTAAdd to basket

Also known as: acb

The sequence of this isoform differs from the canonical sequence as follows:
     516-525: Missing.
     636-640: SFTEN → DQAENEDGAQENTF

Note: Expressed in lung.
Show »
Length:868
Mass (Da):97,821
Checksum:iF47C9B5D47E6146B
GO
Isoform 12 (identifier: Q08877-13) [UniParc]FASTAAdd to basket

Also known as: aca

The sequence of this isoform differs from the canonical sequence as follows:
     516-525: Missing.
     636-640: SFTEN → DQAENEDGAQENTF
     847-869: SRRPPPSPTRPTIIRPLESSLLD → RFGAVKEEAVEP

Note: Expressed in lung.
Show »
Length:857
Mass (Da):96,566
Checksum:iC348C4D220A8E4B5
GO
Isoform 13 (identifier: Q08877-8) [UniParc]FASTAAdd to basket

Also known as: c

The sequence of this isoform differs from the canonical sequence as follows:
     526-564: VIRKGWLTVS...SLSWYKDDEE → VRAKFCDSEG...CPHGRKLVLV
     565-869: Missing.

Note: Expressed in lung, brain, heart, testis.
Show »
Length:564
Mass (Da):63,927
Checksum:i2EE4B6A6D27E35CB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei516 – 52510Missing in isoform 7, isoform 8, isoform 9, isoform 10, isoform 11 and isoform 12. 1 PublicationVSP_034038
Alternative sequencei526 – 56439VIRKG…KDDEE → VRAKFCDSEGLADRQQHWHH ERRLEGLLVCPHGRKLVLV in isoform 13. CuratedVSP_034039Add
BLAST
Alternative sequencei565 – 869305Missing in isoform 13. CuratedVSP_034040Add
BLAST
Alternative sequencei636 – 6405SFTEN → SFGSNKTEM in isoform 3, isoform 4, isoform 9 and isoform 10. CuratedVSP_034041
Alternative sequencei636 – 6405SFTEN → DQAENEDGAQENTF in isoform 5, isoform 6, isoform 11 and isoform 12. CuratedVSP_034042
Alternative sequencei847 – 86923SRRPP…SSLLD → RFGAVKEEAVEP in isoform 2, isoform 4, isoform 6, isoform 8, isoform 10 and isoform 12. 1 PublicationVSP_034043Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14076 mRNA. Translation: BAA03161.1.
AF201839 mRNA. Translation: AAF07848.1.
PIRiI55498.
RefSeqiNP_612547.1. NM_138538.1. [Q08877-1]
XP_006250203.1. XM_006250141.2. [Q08877-7]
XP_006250204.1. XM_006250142.2. [Q08877-9]
UniGeneiRn.11191.

Genome annotation databases

EnsembliENSRNOT00000067653; ENSRNOP00000063767; ENSRNOG00000026490. [Q08877-1]
ENSRNOT00000075938; ENSRNOP00000068044; ENSRNOG00000026490. [Q08877-9]
GeneIDi171574.
KEGGirno:171574.
UCSCiRGD:727949. rat. [Q08877-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14076 mRNA. Translation: BAA03161.1.
AF201839 mRNA. Translation: AAF07848.1.
PIRiI55498.
RefSeqiNP_612547.1. NM_138538.1. [Q08877-1]
XP_006250203.1. XM_006250141.2. [Q08877-7]
XP_006250204.1. XM_006250142.2. [Q08877-9]
UniGeneiRn.11191.

3D structure databases

ProteinModelPortaliQ08877.
SMRiQ08877. Positions 6-304, 525-635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251309. 2 interactions.
IntActiQ08877. 2 interactions.
STRINGi10116.ENSRNOP00000060160.

PTM databases

iPTMnetiQ08877.
PhosphoSiteiQ08877.

Proteomic databases

PaxDbiQ08877.
PRIDEiQ08877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067653; ENSRNOP00000063767; ENSRNOG00000026490. [Q08877-1]
ENSRNOT00000075938; ENSRNOP00000068044; ENSRNOG00000026490. [Q08877-9]
GeneIDi171574.
KEGGirno:171574.
UCSCiRGD:727949. rat. [Q08877-1]

Organism-specific databases

CTDi26052.
RGDi727949. Dnm3.

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
GeneTreeiENSGT00760000119213.
HOGENOMiHOG000161069.
HOVERGENiHBG107833.
InParanoidiQ08877.
KOiK01528.
PhylomeDBiQ08877.

Enzyme and pathway databases

BRENDAi3.6.5.5. 5301.
ReactomeiR-RNO-177504. Retrograde neurotrophin signalling.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-437239. Recycling pathway of L1.

Miscellaneous databases

PROiQ08877.

Gene expression databases

GenevisibleiQ08877. RN.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel member of the dynamin family of GTP-binding proteins is expressed specifically in the testis."
    Nakata T., Takamura R., Hirokawa N.
    J. Cell Sci. 105:1-5(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 8), TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Three dynamin-encoding genes are differentially expressed in developing rat brain."
    Cook T., Mesa K., Urrutia R.
    J. Neurochem. 67:927-931(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
    Tissue: Brain.
  3. Cited for: PROTEIN SEQUENCE OF 839-859 (ISOFORM 1/3/5/7/9/11), PHOSPHORYLATION AT SER-769; SER-773 AND SER-853, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Differential distribution of dynamin isoforms in mammalian cells."
    Cao H., Garcia F., McNiven M.A.
    Mol. Biol. Cell 9:2595-2609(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-769 AND SER-773, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDYN3_RAT
AccessioniPrimary (citable) accession number: Q08877
Secondary accession number(s): Q9QXL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: June 10, 2008
Last modified: July 6, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.