Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q08873 (SCP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transgelin
Alternative name(s):
Calponin homolog 1
Gene names
Name:SCP1
Ordered Locus Names:YOR367W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has actin-binding and actin-bundling activity. Stabilizes actin filaments against disassembly.

Subunit structure

Binds to actin. Interacts with ABP1. Ref.4 Ref.5 Ref.7

Subcellular location

Cytoplasmcytoskeletonactin patch. Note: Cortical actin patches. Ref.3 Ref.4 Ref.5

Miscellaneous

Present with 1578 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 CH (calponin-homology) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ABP1P158916EBI-33137,EBI-2036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 200199Transgelin
PRO_0000204795

Regions

Domain26 – 136111CH
Region151 – 16414Interaction with SH3 domain of ABP1

Amino acid modifications

Modified residue21N-acetylserine Ref.11
Modified residue111Phosphoserine Ref.8 Ref.10

Experimental info

Mutagenesis1851S → A: Greatly reduces actin-binding and actin-bundling activity. Ref.5
Mutagenesis1851S → D: Moderately reduces actin-binding and actin-bundling activity. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q08873 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6C8E7EACCFD0DE28

FASTA20022,748
        10         20         30         40         50         60 
MSYDKKADVT SLDEDLRQLR ESKFSPEAIQ NIKIWVYKSV LKEIAPPGDL LECLKDGTVL 

        70         80         90        100        110        120 
CKLANILYEA DTGEANHISW KSSKMPFVQM DQISQFLSFS RKYGVPEDEL FQTIDLFEKK 

       130        140        150        160        170        180 
DPAIVFQTLK SLSRYANKKH TDRFPVLGPQ LSTKKPRPPV KSKPKHLQDG TGWSTFEYGY 

       190        200 
MKGASQATEG VVLGQRRDIV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"SCP1 encodes an actin-bundling protein in yeast."
Winder S.J., Jess T., Ayscough K.R.
Biochem. J. 375:287-295(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ACTIN, SUBCELLULAR LOCATION.
[5]"The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton."
Goodman A., Goode B.L., Matsudaira P., Fink G.R.
Mol. Biol. Cell 14:2617-2629(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ACTIN, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-185.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Protein interaction networks by proteome peptide scanning."
Landgraf C., Panni S., Montecchi-Palazzi L., Castagnoli L., Schneider-Mergener J., Volkmer-Engert R., Cesareni G.
PLoS Biol. 2:94-103(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ABP1.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z75275 Genomic DNA. Translation: CAA99698.1.
BK006948 Genomic DNA. Translation: DAA11126.1.
PIRS67279.
RefSeqNP_015012.3. NM_001183787.3.

3D structure databases

ProteinModelPortalQ08873.
SMRQ08873. Positions 11-157.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34750. 11 interactions.
DIPDIP-6340N.
IntActQ08873. 2 interactions.
MINTMINT-686883.
STRING4932.YOR367W.

Proteomic databases

PaxDbQ08873.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR367W; YOR367W; YOR367W.
GeneID854549.
KEGGsce:YOR367W.

Organism-specific databases

CYGDYOR367w.
SGDS000005894. SCP1.

Phylogenomic databases

eggNOGCOG5199.
GeneTreeENSGT00550000074447.
HOGENOMHOG000232113.
OMALNLQMGF.
OrthoDBEOG754J1V.

Enzyme and pathway databases

BioCycYEAST:G3O-33835-MONOMER.

Gene expression databases

GenevestigatorQ08873.

Family and domain databases

Gene3D1.10.418.10. 1 hit.
InterProIPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamPF00307. CH. 1 hit.
[Graphical view]
PRINTSPR00888. SM22CALPONIN.
SMARTSM00033. CH. 1 hit.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
PROSITEPS50021. CH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976964.
PROQ08873.

Entry information

Entry nameSCP1_YEAST
AccessionPrimary (citable) accession number: Q08873
Secondary accession number(s): D6W360
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families