Q08873 (SCP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transgelin

Alternative name(s):
Calponin homolog 1

Gene names

Name:	SCP1
Ordered Locus Names:	YOR367W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has actin-binding and actin-bundling activity. Stabilizes actin filaments against disassembly.
Subunit structure	Binds to actin. Interacts with ABP1. Ref.4 Ref.5 Ref.7
Subcellular location	Cytoplasm › cytoskeleton › actin patch. Note: Cortical actin patches. Ref.3 Ref.4 Ref.5
Miscellaneous	Present with 1578 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 CH (calponin-homology) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from direct assay Ref.5. Source: SGD chronological cell aging Inferred from mutant phenotype PubMed 15024029. Source: SGD
Cellular_component	actin cortical patch Inferred from direct assay Ref.5. Source: SGD
Molecular_function	actin filament binding Inferred from direct assay Ref.5. Source: SGD protein binding, bridging Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABP1	P15891	5	EBI-33137,EBI-2036

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Transgelin		PRO_0000204795
Regions
	Domain	26 – 136	111	CH
	Region	151 – 164	14	Interaction with SH3 domain of ABP1
Amino acid modifications
	Modified residue	10	1	Phosphothreonine Ref.10
	Modified residue	11	1	Phosphoserine Ref.8 Ref.9 Ref.10
Experimental info
	Mutagenesis	185	1	S → A: Greatly reduces actin-binding and actin-bundling activity. Ref.5
	Mutagenesis	185	1	S → D: Moderately reduces actin-binding and actin-bundling activity. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q08873 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6C8E7EACCFD0DE28	FASTA	200	22,748
	10 20 30 40 50 60 MSYDKKADVT SLDEDLRQLR ESKFSPEAIQ NIKIWVYKSV LKEIAPPGDL LECLKDGTVL 70 80 90 100 110 120 CKLANILYEA DTGEANHISW KSSKMPFVQM DQISQFLSFS RKYGVPEDEL FQTIDLFEKK 130 140 150 160 170 180 DPAIVFQTLK SLSRYANKKH TDRFPVLGPQ LSTKKPRPPV KSKPKHLQDG TGWSTFEYGY 190 200 MKGASQATEG VVLGQRRDIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"SCP1 encodes an actin-bundling protein in yeast." Winder S.J., Jess T., Ayscough K.R. Biochem. J. 375:287-295(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACTIN, SUBCELLULAR LOCATION.
[5]	"The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton." Goodman A., Goode B.L., Matsudaira P., Fink G.R. Mol. Biol. Cell 14:2617-2629(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACTIN, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-185.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Protein interaction networks by proteome peptide scanning." Landgraf C., Panni S., Montecchi-Palazzi L., Castagnoli L., Schneider-Mergener J., Volkmer-Engert R., Cesareni G. PLoS Biol. 2:94-103(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ABP1.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-10 AND SER-11, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75275 Genomic DNA. Translation: CAA99698.1. BK006948 Genomic DNA. Translation: DAA11126.1.
PIR	S67279.
RefSeq	NP_015012.3. NM_001183787.3.
3D structure databases
ProteinModelPortal	Q08873.
SMR	Q08873. Positions 21-144.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6340N.
IntAct	Q08873. 2 interactions.
MINT	MINT-686883.
STRING	4932.YOR367W.
Proteomic databases
PaxDb	Q08873.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR367W; YOR367W; YOR367W.
GeneID	854549.
KEGG	sce:YOR367W. sce:YOR369C.
Organism-specific databases
CYGD	YOR367w.
SGD	S000005894. SCP1.
Phylogenomic databases
eggNOG	COG5199.
GeneTree	ENSGT00550000074447.
HOGENOM	HOG000232113.
KO	K02951.
OMA	CKLANIL.
OrthoDB	EOG45B4R2.
Gene expression databases
Genevestigator	Q08873.
GermOnline	YOR367W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.418.10. 1 hit.
InterPro	IPR001715. CH-domain. IPR003096. SM22_calponin. [Graphical view]
Pfam	PF00307. CH. 1 hit. [Graphical view]
PRINTS	PR00888. SM22CALPONIN.
SMART	SM00033. CH. 1 hit. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit.
PROSITE	PS50021. CH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976964.

Entry information

Entry name

SCP1_YEAST

Accession

Primary (citable) accession number: Q08873
Secondary accession number(s): D6W360

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program