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Q08873

- SCP1_YEAST

UniProt

Q08873 - SCP1_YEAST

Protein

Transgelin

Gene

SCP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Has actin-binding and actin-bundling activity. Stabilizes actin filaments against disassembly.

    GO - Molecular functioni

    1. actin filament binding Source: SGD
    2. protein binding Source: IntAct
    3. protein binding, bridging Source: SGD

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. chronological cell aging Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33835-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transgelin
    Alternative name(s):
    Calponin homolog 1
    Gene namesi
    Name:SCP1
    Ordered Locus Names:YOR367W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR367w.
    SGDiS000005894. SCP1.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 3 Publications
    Note: Cortical actin patches.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi185 – 1851S → A: Greatly reduces actin-binding and actin-bundling activity. 1 Publication
    Mutagenesisi185 – 1851S → D: Moderately reduces actin-binding and actin-bundling activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 200199TransgelinPRO_0000204795Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei11 – 111Phosphoserine2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ08873.
    PaxDbiQ08873.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08873.

    Interactioni

    Subunit structurei

    Binds to actin. Interacts with ABP1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P158916EBI-33137,EBI-2036

    Protein-protein interaction databases

    BioGridi34750. 11 interactions.
    DIPiDIP-6340N.
    IntActiQ08873. 2 interactions.
    MINTiMINT-686883.
    STRINGi4932.YOR367W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08873.
    SMRiQ08873. Positions 22-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 136111CHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni151 – 16414Interaction with SH3 domain of ABP1Add
    BLAST

    Sequence similaritiesi

    Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5199.
    GeneTreeiENSGT00550000074447.
    HOGENOMiHOG000232113.
    OMAiLNLQMGF.
    OrthoDBiEOG754J1V.

    Family and domain databases

    Gene3Di1.10.418.10. 1 hit.
    InterProiIPR001715. CH-domain.
    IPR003096. SM22_calponin.
    [Graphical view]
    PfamiPF00307. CH. 1 hit.
    [Graphical view]
    PRINTSiPR00888. SM22CALPONIN.
    SMARTiSM00033. CH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    PROSITEiPS50021. CH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08873-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYDKKADVT SLDEDLRQLR ESKFSPEAIQ NIKIWVYKSV LKEIAPPGDL    50
    LECLKDGTVL CKLANILYEA DTGEANHISW KSSKMPFVQM DQISQFLSFS 100
    RKYGVPEDEL FQTIDLFEKK DPAIVFQTLK SLSRYANKKH TDRFPVLGPQ 150
    LSTKKPRPPV KSKPKHLQDG TGWSTFEYGY MKGASQATEG VVLGQRRDIV 200
    Length:200
    Mass (Da):22,748
    Last modified:November 1, 1996 - v1
    Checksum:i6C8E7EACCFD0DE28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z75275 Genomic DNA. Translation: CAA99698.1.
    BK006948 Genomic DNA. Translation: DAA11126.1.
    PIRiS67279.
    RefSeqiNP_015012.3. NM_001183787.3.

    Genome annotation databases

    EnsemblFungiiYOR367W; YOR367W; YOR367W.
    GeneIDi854549.
    KEGGisce:YOR367W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z75275 Genomic DNA. Translation: CAA99698.1 .
    BK006948 Genomic DNA. Translation: DAA11126.1 .
    PIRi S67279.
    RefSeqi NP_015012.3. NM_001183787.3.

    3D structure databases

    ProteinModelPortali Q08873.
    SMRi Q08873. Positions 22-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34750. 11 interactions.
    DIPi DIP-6340N.
    IntActi Q08873. 2 interactions.
    MINTi MINT-686883.
    STRINGi 4932.YOR367W.

    Proteomic databases

    MaxQBi Q08873.
    PaxDbi Q08873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR367W ; YOR367W ; YOR367W .
    GeneIDi 854549.
    KEGGi sce:YOR367W.

    Organism-specific databases

    CYGDi YOR367w.
    SGDi S000005894. SCP1.

    Phylogenomic databases

    eggNOGi COG5199.
    GeneTreei ENSGT00550000074447.
    HOGENOMi HOG000232113.
    OMAi LNLQMGF.
    OrthoDBi EOG754J1V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33835-MONOMER.

    Miscellaneous databases

    NextBioi 976964.
    PROi Q08873.

    Gene expression databases

    Genevestigatori Q08873.

    Family and domain databases

    Gene3Di 1.10.418.10. 1 hit.
    InterProi IPR001715. CH-domain.
    IPR003096. SM22_calponin.
    [Graphical view ]
    Pfami PF00307. CH. 1 hit.
    [Graphical view ]
    PRINTSi PR00888. SM22CALPONIN.
    SMARTi SM00033. CH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    PROSITEi PS50021. CH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. "SCP1 encodes an actin-bundling protein in yeast."
      Winder S.J., Jess T., Ayscough K.R.
      Biochem. J. 375:287-295(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ACTIN, SUBCELLULAR LOCATION.
    5. "The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton."
      Goodman A., Goode B.L., Matsudaira P., Fink G.R.
      Mol. Biol. Cell 14:2617-2629(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ACTIN, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-185.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: INTERACTION WITH ABP1.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSCP1_YEAST
    AccessioniPrimary (citable) accession number: Q08873
    Secondary accession number(s): D6W360
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1578 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3