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Protein

Platelet glycoprotein 4

Gene

Cd36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei463 – 4631Critical for TLR4-TLR6 dimerization and signalingBy similarity

GO - Molecular functioni

  • high-density lipoprotein particle binding Source: MGI
  • lipid binding Source: BHF-UCL
  • lipoprotein particle binding Source: MGI
  • lipoteichoic acid receptor activity Source: MGI
  • low-density lipoprotein particle binding Source: BHF-UCL
  • low-density lipoprotein receptor activity Source: BHF-UCL

GO - Biological processi

  • apoptotic cell clearance Source: MGI
  • cell adhesion Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: BHF-UCL
  • cellular response to bacterial lipopeptide Source: MGI
  • cellular response to hydroperoxide Source: BHF-UCL
  • cellular response to lipopolysaccharide Source: MGI
  • cellular response to lipoteichoic acid Source: MGI
  • cGMP-mediated signaling Source: MGI
  • cholesterol transport Source: BHF-UCL
  • defense response to Gram-positive bacterium Source: MGI
  • lipid storage Source: BHF-UCL
  • lipoprotein transport Source: BHF-UCL
  • long-chain fatty acid import Source: MGI
  • low-density lipoprotein particle clearance Source: BHF-UCL
  • low-density lipoprotein particle mediated signaling Source: BHF-UCL
  • negative regulation of gene expression Source: BHF-UCL
  • negative regulation of growth of symbiont in host Source: MGI
  • negative regulation of transcription factor import into nucleus Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • nitric oxide mediated signal transduction Source: MGI
  • pattern recognition receptor signaling pathway Source: GOC
  • phagocytosis, recognition Source: MGI
  • plasma lipoprotein particle clearance Source: BHF-UCL
  • plasma membrane long-chain fatty acid transport Source: MGI
  • positive regulation of blood coagulation Source: BHF-UCL
  • positive regulation of blood microparticle formation Source: BHF-UCL
  • positive regulation of cell-matrix adhesion Source: MGI
  • positive regulation of cholesterol storage Source: BHF-UCL
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • positive regulation of interleukin-12 production Source: MGI
  • positive regulation of interleukin-6 production Source: MGI
  • positive regulation of macrophage cytokine production Source: MGI
  • positive regulation of macrophage derived foam cell differentiation Source: BHF-UCL
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
  • positive regulation of phagocytosis, engulfment Source: MGI
  • positive regulation of reactive oxygen species metabolic process Source: BHF-UCL
  • positive regulation of tumor necrosis factor production Source: MGI
  • receptor-mediated endocytosis Source: GOC
  • regulation of removal of superoxide radicals Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: BHF-UCL
  • response to stilbenoid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Cell adhesion, Transport

Enzyme and pathway databases

ReactomeiREACT_281935. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_282527. Scavenging by Class B Receptors.
REACT_307071. Platelet degranulation.
REACT_309099. MyD88:Mal cascade initiated on plasma membrane.
REACT_321869. Cross-presentation of particulate exogenous antigens (phagosomes).

Protein family/group databases

TCDBi9.B.39.1.1. the long chain fatty acid translocase (lcfat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet glycoprotein 4
Alternative name(s):
Glycoprotein IIIb
Short name:
GPIIIB
PAS IV
PAS-4
Platelet glycoprotein IV
Short name:
GPIV
CD_antigen: CD36
Gene namesi
Name:Cd36
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:107899. Cd36.

Subcellular locationi

  • Cell membrane; Multi-pass membrane protein

  • Note: Upon ligand-binding, internalized through dynamin-dependent endocytosis.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 76CytoplasmicSequence Analysis
Transmembranei8 – 2922HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 439410ExtracellularSequence AnalysisAdd
BLAST
Transmembranei440 – 46122HelicalSequence AnalysisAdd
BLAST
Topological domaini462 – 47211CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: BHF-UCL
  • external side of plasma membrane Source: MGI
  • extracellular space Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane raft Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Animals with a double knockout of APOE and CD36, fed a Western diet for 12 weeks, exhibit much lower levels of CXCL1, CXCL2 and CCL5 cytokine mRNA expression in the descending aorta and a corresponding decrease in atherosclerotic lesion formation, compared to APOE single knockout mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 472471Platelet glycoprotein 4PRO_0000144153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Lipidationi7 – 71S-palmitoyl cysteineBy similarity
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi243 ↔ 311By similarity
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi272 ↔ 333By similarity
Disulfide bondi313 ↔ 322By similarity
Glycosylationi321 – 3211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis
Lipidationi464 – 4641S-palmitoyl cysteineBy similarity
Lipidationi466 – 4661S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ08857.
PaxDbiQ08857.
PRIDEiQ08857.

Expressioni

Gene expression databases

BgeeiQ08857.
CleanExiMM_CD36.
ExpressionAtlasiQ08857. baseline and differential.
GenevisibleiQ08857. MM.

Interactioni

Subunit structurei

Interacts with THBS1 and THBS2; the interactions mediate the THBS antiangiogenic activity. Upon interaction with a ligand, such as oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42, rapidly forms a complex with TLR4 and TLR6; the complex is internalized and triggers an inflammatory signal. Through its C-terminus, interacts with PTK2, PXN and LYN, but not with SRC. LYN kinase activity is required for facilitating TLR4-TLR6 heterodimerization and signal initiation.By similarity1 Publication

Protein-protein interaction databases

BioGridi198587. 1 interaction.
IntActiQ08857. 4 interactions.
MINTiMINT-4090233.
STRINGi10090.ENSMUSP00000080974.

Structurei

3D structure databases

ProteinModelPortaliQ08857.
SMRiQ08857. Positions 40-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 12028Required for interaction with thrombospondins, THBS1 and THBS2Add
BLAST
Regioni460 – 47213Interaction with PTK2, PXN and LYNBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CD36 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257244.
HOGENOMiHOG000252951.
HOVERGENiHBG002754.
InParanoidiQ08857.
KOiK06259.
OMAiKRNYIVP.
OrthoDBiEOG79SDWX.
PhylomeDBiQ08857.
TreeFamiTF317925.

Family and domain databases

InterProiIPR002159. CD36.
IPR005428. CD36_antigen.
[Graphical view]
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiPF01130. CD36. 1 hit.
[Graphical view]
PRINTSiPR01610. CD36ANTIGEN.
PR01609. CD36FAMILY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCDRNCGLI AGAVIGAVLA VFGGILMPVG DMLIEKTIKR EVVLEEGTTA
60 70 80 90 100
FKNWVKTGTT VYRQFWIFDV QNPDDVAKNS SKIKVKQRGP YTYRVRYLAK
110 120 130 140 150
ENITQDPEDH TVSFVQPNGA IFEPSLSVGT EDDNFTVLNL AVAAAPHIYQ
160 170 180 190 200
NSFVQVVLNS LIKKSKSSMF QTRSLKELLW GYKDPFLSLV PYPISTTVGV
210 220 230 240 250
FYPYNDTVDG VYKVFNGKDN ISKVAIIESY KGKRNLSYWP SYCDMINGTD
260 270 280 290 300
AASFPPFVEK SRTLRFFSSD ICRSIYAVFG SEIDLKGIPV YRFVLPANAF
310 320 330 340 350
ASPLQNPDNH CFCTEKVISN NCTSYGVLDI GKCKEGKPVY ISLPHFLHAS
360 370 380 390 400
PDVSEPIEGL HPNEDEHRTY LDVEPITGFT LQFAKRLQVN ILVKPARKIE
410 420 430 440 450
ALKNLKRPYI VPILWLNETG TIGDEKAEMF KTQVTGKIKL LGMVEMALLG
460 470
IGVVMFVAFM ISYCACKSKN GK
Length:472
Mass (Da):52,698
Last modified:January 23, 2007 - v2
Checksum:i80AEABB18206534F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23108 mRNA. Translation: AAA53028.1.
BC010262 mRNA. Translation: AAH10262.1.
CCDSiCCDS19100.1.
PIRiI49590.
RefSeqiNP_001153027.1. NM_001159555.1.
NP_001153028.1. NM_001159556.1.
NP_001153029.1. NM_001159557.1.
NP_001153030.1. NM_001159558.1.
NP_031669.3. NM_007643.4.
XP_006535683.1. XM_006535620.2.
XP_006535684.1. XM_006535621.2.
XP_006535685.1. XM_006535622.2.
XP_006535686.1. XM_006535623.2.
XP_006535687.1. XM_006535624.2.
XP_006535688.1. XM_006535625.1.
UniGeneiMm.18628.
Mm.406799.

Genome annotation databases

EnsembliENSMUST00000082367; ENSMUSP00000080974; ENSMUSG00000002944.
ENSMUST00000165232; ENSMUSP00000126300; ENSMUSG00000002944.
ENSMUST00000169095; ENSMUSP00000131832; ENSMUSG00000002944.
ENSMUST00000170051; ENSMUSP00000133008; ENSMUSG00000002944.
GeneIDi12491.
KEGGimmu:12491.
UCSCiuc008wnn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23108 mRNA. Translation: AAA53028.1.
BC010262 mRNA. Translation: AAH10262.1.
CCDSiCCDS19100.1.
PIRiI49590.
RefSeqiNP_001153027.1. NM_001159555.1.
NP_001153028.1. NM_001159556.1.
NP_001153029.1. NM_001159557.1.
NP_001153030.1. NM_001159558.1.
NP_031669.3. NM_007643.4.
XP_006535683.1. XM_006535620.2.
XP_006535684.1. XM_006535621.2.
XP_006535685.1. XM_006535622.2.
XP_006535686.1. XM_006535623.2.
XP_006535687.1. XM_006535624.2.
XP_006535688.1. XM_006535625.1.
UniGeneiMm.18628.
Mm.406799.

3D structure databases

ProteinModelPortaliQ08857.
SMRiQ08857. Positions 40-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198587. 1 interaction.
IntActiQ08857. 4 interactions.
MINTiMINT-4090233.
STRINGi10090.ENSMUSP00000080974.

Chemistry

ChEMBLiCHEMBL2176845.

Protein family/group databases

TCDBi9.B.39.1.1. the long chain fatty acid translocase (lcfat) family.

Proteomic databases

MaxQBiQ08857.
PaxDbiQ08857.
PRIDEiQ08857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082367; ENSMUSP00000080974; ENSMUSG00000002944.
ENSMUST00000165232; ENSMUSP00000126300; ENSMUSG00000002944.
ENSMUST00000169095; ENSMUSP00000131832; ENSMUSG00000002944.
ENSMUST00000170051; ENSMUSP00000133008; ENSMUSG00000002944.
GeneIDi12491.
KEGGimmu:12491.
UCSCiuc008wnn.2. mouse.

Organism-specific databases

CTDi948.
MGIiMGI:107899. Cd36.

Phylogenomic databases

eggNOGiNOG257244.
HOGENOMiHOG000252951.
HOVERGENiHBG002754.
InParanoidiQ08857.
KOiK06259.
OMAiKRNYIVP.
OrthoDBiEOG79SDWX.
PhylomeDBiQ08857.
TreeFamiTF317925.

Enzyme and pathway databases

ReactomeiREACT_281935. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_282527. Scavenging by Class B Receptors.
REACT_307071. Platelet degranulation.
REACT_309099. MyD88:Mal cascade initiated on plasma membrane.
REACT_321869. Cross-presentation of particulate exogenous antigens (phagosomes).

Miscellaneous databases

NextBioi281412.
PROiQ08857.
SOURCEiSearch...

Gene expression databases

BgeeiQ08857.
CleanExiMM_CD36.
ExpressionAtlasiQ08857. baseline and differential.
GenevisibleiQ08857. MM.

Family and domain databases

InterProiIPR002159. CD36.
IPR005428. CD36_antigen.
[Graphical view]
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiPF01130. CD36. 1 hit.
[Graphical view]
PRINTSiPR01610. CD36ANTIGEN.
PR01609. CD36FAMILY.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CD36 is a receptor for oxidized low density lipoprotein."
    Endemann G., Stanton L.W., Madden K.S., Bryant C.M., White R.T., Protter A.A.
    J. Biol. Chem. 268:11811-11816(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Peritoneal macrophage.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  3. "The antiangiogenic effect of thrombospondin-2 is mediated by CD36 and modulated by histidine-rich glycoprotein."
    Simantov R., Febbraio M., Silverstein R.L.
    Matrix Biol. 24:27-34(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THBS2, FUNCTION.
  4. "CD36 ligands promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer."
    Stewart C.R., Stuart L.M., Wilkinson K., van Gils J.M., Deng J., Halle A., Rayner K.J., Boyer L., Zhong R., Frazier W.A., Lacy-Hulbert A., El Khoury J., Golenbock D.T., Moore K.J.
    Nat. Immunol. 11:155-161(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCD36_MOUSE
AccessioniPrimary (citable) accession number: Q08857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.