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Q08851 (STX5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-5
Gene names
Name:Stx5
Synonyms:Stx5a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates endoplasmic reticulum to Golgi transport. Ref.4 Ref.5

Subunit structure

Part of a ternary complex containing STX5A, NSFL1C and VCP. Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR1, GOSR2 and YKT6. This complex also includes VTI1A By similarity. Interacts with COG4 By similarity. Ref.4 Ref.5 Ref.6

Subcellular location

Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein Potential Ref.3 Ref.6.

Tissue specificity

Expressed in the brain, heart, spleen, lung, liver, kidney and testis. Ref.3

Sequence similarities

Belongs to the syntaxin family.

Contains 1 t-SNARE coiled-coil homology domain.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q08851-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q08851-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.
Note: Produced by alternative initiation at Met-55 of isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Syntaxin-5
PRO_0000210207

Regions

Topological domain1 – 333333Cytoplasmic Potential
Transmembrane334 – 35421Helical; Anchor for type IV membrane protein; Potential
Topological domain3551Vesicular Potential
Domain263 – 32563t-SNARE coiled-coil homology
Coiled coil287 – 31832 Potential

Natural variations

Alternative sequence1 – 5454Missing in isoform 2.
VSP_020117

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: 0B0D0B2C261A39B4

FASTA35539,751
        10         20         30         40         50         60 
MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AINSSSDIAP LPTPVALVPS PPDTMSCRDR 

        70         80         90        100        110        120 
TQEFLSACKS LQSRQNGIQT NKPALHATRQ CSEFTLMARR IGKDLSNTFA KLEKLTILAK 

       130        140        150        160        170        180 
RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ 

       190        200        210        220        230        240 
SKLASMSNDF KSVLEVRTEN LKQQRNRREQ FSRAPVSALP LAPNNLGGGP IVLGGESRAS 

       250        260        270        280        290        300 
RDVAIDMMDP RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET 

       310        320        330        340        350 
IQRIDENVLG AQLDVEAAHS EILKYFQSVT SNRWLMVKIF LILIVFFIIF VVFLA

« Hide

Isoform 2 [UniParc].

Checksum: F560A1A079C8A9F7
Show »

FASTA30134,117

References

« Hide 'large scale' references
[1]"The syntaxin family of vesicular transport receptors."
Bennett M.K., Garcia-Arraras J.E., Elferink L.A., Peterson K.E., Fleming A.M., Hazuka C.D., Scheller R.H.
Cell 74:863-873(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Spleen.
[3]"An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal."
Hui N., Nakamura N., Sonnichsen B., Shima D.T., Nilsson T., Warren G.
Mol. Biol. Cell 8:1777-1787(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-211 (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE INITIATION.
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro."
Rabouille C., Kondo H., Newman R., Hui N., Freemont P., Warren G.
Cell 92:603-610(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VCP AND NSFL1C.
[5]"Role of p97 and syntaxin 5 in the assembly of transitional endoplasmic reticulum."
Roy L., Bergeron J.J.M., Lavoie C., Hendriks R., Gushue J., Fazel A., Pelletier A., Morre D.J., Subramaniam V.N., Hong W., Paiement J.
Mol. Biol. Cell 11:2529-2542(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VCP AND NSFL1C.
[6]"Ykt6 forms a SNARE complex with syntaxin 5, GS28, and Bet1 and participates in a late stage in endoplasmic reticulum-Golgi transport."
Zhang T., Hong W.
J. Biol. Chem. 276:27480-27487(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GOSR1; GOSR2 AND YKT6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L20822 mRNA. Translation: AAA03047.1.
BC127489 mRNA. Translation: AAI27490.1.
U87971 mRNA. Translation: AAB93844.1.
IPIIPI00204740.
IPI00785588.
PIRF48213.
RefSeqNP_113892.1. NM_031704.1.
UniGeneRn.5782.

3D structure databases

ProteinModelPortalQ08851.
ModBaseSearch...

Protein-protein interaction databases

IntActQ08851. 1 interaction.

Proteomic databases

PaxDbQ08851.
PRIDEQ08851.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025664; ENSRNOP00000025664; ENSRNOG00000018847.
GeneID65134.
KEGGrno:65134.
UCSCRGD:68426. rat.

Organism-specific databases

CTD6811.
RGD68426. Stx5.

Phylogenomic databases

eggNOGNOG326964.
GeneTreeENSGT00390000000652.
HOGENOMHOG000194860.
HOVERGENHBG001973.
InParanoidQ08851.
KOK08490.
OMATHSATVV.
OrthoDBEOG4S7JQD.

Gene expression databases

GenevestigatorQ08851.
GermOnlineENSRNOG00000018847. Rattus norvegicus.

Family and domain databases

InterProIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMSSF47661. t-snare. 1 hit.
PROSITEPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613943.
PMAP-CutDBQ08851.

Entry information

Entry nameSTX5_RAT
AccessionPrimary (citable) accession number: Q08851
Secondary accession number(s): A0JPL4, O55200
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 5, 2006
Last modified: April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents