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Q08850

- STX4_RAT

UniProt

Q08850 - STX4_RAT

Protein

Syntaxin-4

Gene

Stx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes By similarity. May also play a role in docking of synaptic vesicles at presynaptic active zones.By similarity

    GO - Molecular functioni

    1. protein binding Source: RGD
    2. Rab GTPase binding Source: RGD
    3. SNAP receptor activity Source: FlyBase
    4. SNARE binding Source: RGD
    5. spectrin binding Source: RGD

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. intracellular transport Source: RGD
    3. membrane fusion Source: RGD
    4. protein complex assembly Source: RGD
    5. synaptic vesicle docking involved in exocytosis Source: RGD

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-4
    Gene namesi
    Name:Stx4
    Synonyms:Stx4a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621019. Stx4.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. basolateral plasma membrane Source: RGD
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: RGD
    5. sarcolemma Source: RGD
    6. SNARE complex Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Syntaxin-4PRO_0000210204Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151PhosphoserineBy similarity
    Modified residuei29 – 291PhosphoserineBy similarity
    Modified residuei117 – 1171PhosphoserineBy similarity
    Modified residuei248 – 2481PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ08850.
    PRIDEiQ08850.

    PTM databases

    PhosphoSiteiQ08850.

    Expressioni

    Tissue specificityi

    Expressed in all tissues tested inluding adipose, brain, testis, intestine, liver, heart, spleen, skeletal muscle and kidney.2 Publications

    Gene expression databases

    GenevestigatoriQ08850.

    Interactioni

    Subunit structurei

    Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with SNAP23 and SNAPIN. Interacts with VAMP2. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 By similarity. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Interacts with STXBP6.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi249661. 2 interactions.
    DIPiDIP-373N.
    IntActiQ08850. 7 interactions.
    MINTiMINT-1509132.
    STRINGi10116.ENSRNOP00000026224.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08850.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 274274CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini296 – 2983ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei275 – 29521Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini200 – 26263t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni154 – 298145Interaction with CENPFBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili38 – 163126Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the syntaxin family.Curated
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5074.
    HOGENOMiHOG000286023.
    HOVERGENiHBG000497.
    InParanoidiQ08850.
    KOiK13502.
    PhylomeDBiQ08850.

    Family and domain databases

    InterProiIPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PfamiPF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view]
    SMARTiSM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47661. SSF47661. 1 hit.
    PROSITEiPS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08850-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLSSPDDE FFQKVQTIRQ    50
    TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ 100
    LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE 150
    KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL 200
    NEISARHSEI QQLERTIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD 250
    YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG 298
    Length:298
    Mass (Da):34,209
    Last modified:November 1, 1996 - v1
    Checksum:i9E854270DFB3CB96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20821 mRNA. Translation: AAA03046.1.
    PIRiE48213.
    RefSeqiNP_112387.1. NM_031125.1.
    UniGeneiRn.33218.

    Genome annotation databases

    GeneIDi81803.
    KEGGirno:81803.
    UCSCiRGD:621019. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20821 mRNA. Translation: AAA03046.1 .
    PIRi E48213.
    RefSeqi NP_112387.1. NM_031125.1.
    UniGenei Rn.33218.

    3D structure databases

    ProteinModelPortali Q08850.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249661. 2 interactions.
    DIPi DIP-373N.
    IntActi Q08850. 7 interactions.
    MINTi MINT-1509132.
    STRINGi 10116.ENSRNOP00000026224.

    PTM databases

    PhosphoSitei Q08850.

    Proteomic databases

    PaxDbi Q08850.
    PRIDEi Q08850.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81803.
    KEGGi rno:81803.
    UCSCi RGD:621019. rat.

    Organism-specific databases

    CTDi 6810.
    RGDi 621019. Stx4.

    Phylogenomic databases

    eggNOGi COG5074.
    HOGENOMi HOG000286023.
    HOVERGENi HBG000497.
    InParanoidi Q08850.
    KOi K13502.
    PhylomeDBi Q08850.

    Miscellaneous databases

    NextBioi 615659.
    PROi Q08850.

    Gene expression databases

    Genevestigatori Q08850.

    Family and domain databases

    InterProi IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    Pfami PF05739. SNARE. 1 hit.
    PF00804. Syntaxin. 1 hit.
    [Graphical view ]
    SMARTi SM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47661. SSF47661. 1 hit.
    PROSITEi PS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly."
      Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H.
      J. Biol. Chem. 277:28271-28279(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STXBP6.
    3. "Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis."
      Rao S.K., Huynh C., Proux-Gillardeaux V., Galli T., Andrews N.W.
      J. Biol. Chem. 279:20471-20479(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SNARE COMPLEX CHARACTERIZATION.
    4. "Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport."
      Pooley R.D., Moynihan K.L., Soukoulis V., Reddy S., Francis R., Lo C., Ma L.-J., Bader D.M.
      J. Cell Sci. 121:3413-3421(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSTX4_RAT
    AccessioniPrimary (citable) accession number: Q08850
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3