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Q08850 (STX4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-4
Gene names
Name:Stx4
Synonyms:Stx4a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes By similarity. May also play a role in docking of synaptic vesicles at presynaptic active zones.

Subunit structure

Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with SNAP23 and SNAPIN. Interacts with VAMP2. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 By similarity. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Interacts with STXBP6. Ref.2

Subcellular location

Cell membrane; Single-pass type IV membrane protein Potential.

Tissue specificity

Expressed in all tissues tested inluding adipose, brain, testis, intestine, liver, heart, spleen, skeletal muscle and kidney. Ref.1 Ref.4

Sequence similarities

Belongs to the syntaxin family.

Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCell membrane
Membrane
   DomainCoiled coil
Transmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular protein transport

Inferred from electronic annotation. Source: InterPro

intracellular transport

Non-traceable author statement Ref.1. Source: RGD

membrane fusion

Inferred from direct assay PubMed 19109692. Source: RGD

protein complex assembly

Inferred from direct assay PubMed 10051443. Source: RGD

synaptic vesicle docking involved in exocytosis

Inferred from direct assay Ref.1. Source: RGD

   Cellular_componentSNARE complex

Inferred from direct assay Ref.3. Source: UniProtKB

apical plasma membrane

Inferred from direct assay PubMed 14759605. Source: RGD

basolateral plasma membrane

Inferred from direct assay PubMed 14759605. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 10051443PubMed 16870704. Source: RGD

sarcolemma

Inferred from direct assay PubMed 12388133. Source: RGD

   Molecular_functionRab GTPase binding

Inferred from physical interaction PubMed 11063739. Source: RGD

SNAP receptor activity

Inferred from direct assay PubMed 19109692. Source: FlyBase

SNARE binding

Inferred from physical interaction PubMed 10051443. Source: RGD

protein binding

Inferred from physical interaction PubMed 14759605. Source: RGD

spectrin binding

Inferred from physical interaction PubMed 16870704. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Syntaxin-4
PRO_0000210204

Regions

Topological domain1 – 274274Cytoplasmic Potential
Transmembrane275 – 29521Helical; Anchor for type IV membrane protein; Potential
Topological domain296 – 2983Extracellular Potential
Domain200 – 26263t-SNARE coiled-coil homology
Region154 – 298145Interaction with CENPF By similarity
Coiled coil38 – 163126 Potential

Amino acid modifications

Modified residue151Phosphoserine By similarity
Modified residue291Phosphoserine By similarity
Modified residue1171Phosphoserine By similarity
Modified residue2481Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08850 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9E854270DFB3CB96

FASTA29834,209
        10         20         30         40         50         60 
MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLSSPDDE FFQKVQTIRQ TMAKLESKVR 

        70         80         90        100        110        120 
ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ LKAIEPQKEE ADENYNSVNT 

       130        140        150        160        170        180 
RMKKTQHGVL SQQFVELINK CNSMQSEYRE KNVERIRRQL KITNAGMVSD EELEQMLDSG 

       190        200        210        220        230        240 
QSEVFVSNIL KDTQVTRQAL NEISARHSEI QQLERTIREL HEIFTFLATE VEMQGEMINR 

       250        260        270        280        290 
IEKNILSSAD YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG 

« Hide

References

[1]"The syntaxin family of vesicular transport receptors."
Bennett M.K., Garcia-Arraras J.E., Elferink L.A., Peterson K.E., Fleming A.M., Hazuka C.D., Scheller R.H.
Cell 74:863-873(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly."
Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H.
J. Biol. Chem. 277:28271-28279(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STXBP6.
[3]"Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis."
Rao S.K., Huynh C., Proux-Gillardeaux V., Galli T., Andrews N.W.
J. Biol. Chem. 279:20471-20479(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SNARE COMPLEX CHARACTERIZATION.
[4]"Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport."
Pooley R.D., Moynihan K.L., Soukoulis V., Reddy S., Francis R., Lo C., Ma L.-J., Bader D.M.
J. Cell Sci. 121:3413-3421(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20821 mRNA. Translation: AAA03046.1.
PIRE48213.
RefSeqNP_112387.1. NM_031125.1.
UniGeneRn.33218.

3D structure databases

ProteinModelPortalQ08850.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249661. 2 interactions.
DIPDIP-373N.
IntActQ08850. 7 interactions.
MINTMINT-1509132.
STRING10116.ENSRNOP00000026224.

PTM databases

PhosphoSiteQ08850.

Proteomic databases

PaxDbQ08850.
PRIDEQ08850.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81803.
KEGGrno:81803.
UCSCRGD:621019. rat.

Organism-specific databases

CTD6810.
RGD621019. Stx4.

Phylogenomic databases

eggNOGCOG5074.
HOGENOMHOG000286023.
HOVERGENHBG000497.
InParanoidQ08850.
KOK13502.
PhylomeDBQ08850.

Gene expression databases

GenevestigatorQ08850.

Family and domain databases

InterProIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMSSF47661. SSF47661. 1 hit.
PROSITEPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615659.
PROQ08850.

Entry information

Entry nameSTX4_RAT
AccessionPrimary (citable) accession number: Q08850
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families