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Protein

Syntaxin-4

Gene

Stx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.By similarity

GO - Molecular functioni

  • Rab GTPase binding Source: RGD
  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: RGD
  • spectrin binding Source: RGD

GO - Biological processi

  • intracellular protein transport Source: GO_Central
  • intracellular transport Source: RGD
  • membrane fusion Source: RGD
  • protein complex assembly Source: RGD
  • synaptic vesicle docking Source: RGD
  • synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central

Keywordsi

Biological processNeurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Gene namesi
Name:Stx4
Synonyms:Stx4a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621019. Stx4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 274CytoplasmicSequence analysisAdd BLAST274
Transmembranei275 – 295Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini296 – 298ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102041 – 298Syntaxin-4Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei29PhosphoserineBy similarity1
Modified residuei35PhosphoserineCombined sources1
Modified residuei36PhosphoserineBy similarity1
Modified residuei117PhosphoserineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ08850.
PRIDEiQ08850.

PTM databases

iPTMnetiQ08850.
PhosphoSitePlusiQ08850.

Expressioni

Tissue specificityi

Expressed in all tissues tested including adipose, brain, testis, intestine, liver, heart, spleen, skeletal muscle and kidney.2 Publications

Interactioni

Subunit structurei

Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with SNAP23 and SNAPIN. Interacts with VAMP2. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 (By similarity). Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Interacts with STXBP6.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • Rab GTPase binding Source: RGD
  • SNAP receptor activity Source: FlyBase
  • SNARE binding Source: RGD
  • spectrin binding Source: RGD

Protein-protein interaction databases

BioGridi249661. 2 interactors.
CORUMiQ08850.
DIPiDIP-373N.
IntActiQ08850. 10 interactors.
MINTiMINT-1509132.
STRINGi10116.ENSRNOP00000026224.

Structurei

3D structure databases

ProteinModelPortaliQ08850.
SMRiQ08850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 262t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 298Interaction with CENPFBy similarityAdd BLAST145

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 163Sequence analysisAdd BLAST126

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ08850.
KOiK13502.
PhylomeDBiQ08850.

Family and domain databases

InterProiView protein in InterPro
IPR010989. SNARE.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR000727. T_SNARE_dom.
PfamiView protein in Pfam
PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
SMARTiView protein in SMART
SM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiView protein in PROSITE
PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLSSPDDE FFQKVQTIRQ
60 70 80 90 100
TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERTIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG
Length:298
Mass (Da):34,209
Last modified:November 1, 1996 - v1
Checksum:i9E854270DFB3CB96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20821 mRNA. Translation: AAA03046.1.
PIRiE48213.
RefSeqiNP_112387.1. NM_031125.1.
UniGeneiRn.33218.

Genome annotation databases

GeneIDi81803.
KEGGirno:81803.
UCSCiRGD:621019. rat.

Similar proteinsi

Entry informationi

Entry nameiSTX4_RAT
AccessioniPrimary (citable) accession number: Q08850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families