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Protein

Syntaxin-4

Gene

Stx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.By similarity

GO - Molecular functioni

  1. Rab GTPase binding Source: RGD
  2. SNAP receptor activity Source: FlyBase
  3. SNARE binding Source: RGD
  4. spectrin binding Source: RGD

GO - Biological processi

  1. intracellular protein transport Source: GO_Central
  2. intracellular transport Source: RGD
  3. membrane fusion Source: RGD
  4. protein complex assembly Source: RGD
  5. synaptic vesicle docking Source: RGD
  6. synaptic vesicle fusion to presynaptic membrane Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Gene namesi
Name:Stx4
Synonyms:Stx4a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621019. Stx4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 274274CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei275 – 29521Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini296 – 2983ExtracellularSequence Analysis

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. basolateral plasma membrane Source: RGD
  3. endomembrane system Source: GO_Central
  4. integral component of membrane Source: GO_Central
  5. plasma membrane Source: RGD
  6. sarcolemma Source: RGD
  7. SNARE complex Source: UniProtKB
  8. synaptic vesicle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Syntaxin-4PRO_0000210204Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei29 – 291PhosphoserineBy similarity
Modified residuei117 – 1171PhosphoserineBy similarity
Modified residuei248 – 2481PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ08850.
PRIDEiQ08850.

PTM databases

PhosphoSiteiQ08850.

Expressioni

Tissue specificityi

Expressed in all tissues tested inluding adipose, brain, testis, intestine, liver, heart, spleen, skeletal muscle and kidney.2 Publications

Gene expression databases

GenevestigatoriQ08850.

Interactioni

Subunit structurei

Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with SNAP23 and SNAPIN. Interacts with VAMP2. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 (By similarity). Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Interacts with STXBP6.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Snap23O090444EBI-918243,EBI-1812522From a different organism.
Vamp2P630458EBI-918243,EBI-520880

Protein-protein interaction databases

BioGridi249661. 2 interactions.
DIPiDIP-373N.
IntActiQ08850. 10 interactions.
MINTiMINT-1509132.
STRINGi10116.ENSRNOP00000026224.

Structurei

3D structure databases

ProteinModelPortaliQ08850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini200 – 26263t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 298145Interaction with CENPFBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili38 – 163126Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5074.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ08850.
KOiK13502.
PhylomeDBiQ08850.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLSSPDDE FFQKVQTIRQ
60 70 80 90 100
TMAKLESKVR ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMKKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERTIREL HEIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG
Length:298
Mass (Da):34,209
Last modified:November 1, 1996 - v1
Checksum:i9E854270DFB3CB96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20821 mRNA. Translation: AAA03046.1.
PIRiE48213.
RefSeqiNP_112387.1. NM_031125.1.
UniGeneiRn.33218.

Genome annotation databases

GeneIDi81803.
KEGGirno:81803.
UCSCiRGD:621019. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20821 mRNA. Translation: AAA03046.1.
PIRiE48213.
RefSeqiNP_112387.1. NM_031125.1.
UniGeneiRn.33218.

3D structure databases

ProteinModelPortaliQ08850.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249661. 2 interactions.
DIPiDIP-373N.
IntActiQ08850. 10 interactions.
MINTiMINT-1509132.
STRINGi10116.ENSRNOP00000026224.

PTM databases

PhosphoSiteiQ08850.

Proteomic databases

PaxDbiQ08850.
PRIDEiQ08850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81803.
KEGGirno:81803.
UCSCiRGD:621019. rat.

Organism-specific databases

CTDi6810.
RGDi621019. Stx4.

Phylogenomic databases

eggNOGiCOG5074.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ08850.
KOiK13502.
PhylomeDBiQ08850.

Miscellaneous databases

NextBioi615659.
PROiQ08850.

Gene expression databases

GenevestigatoriQ08850.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly."
    Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H.
    J. Biol. Chem. 277:28271-28279(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STXBP6.
  3. "Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis."
    Rao S.K., Huynh C., Proux-Gillardeaux V., Galli T., Andrews N.W.
    J. Biol. Chem. 279:20471-20479(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SNARE COMPLEX CHARACTERIZATION.
  4. "Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport."
    Pooley R.D., Moynihan K.L., Soukoulis V., Reddy S., Francis R., Lo C., Ma L.-J., Bader D.M.
    J. Cell Sci. 121:3413-3421(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiSTX4_RAT
AccessioniPrimary (citable) accession number: Q08850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.