Q08850 (STX4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-4

Gene names

Name:	Stx4
Synonyms:	Stx4a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes By similarity. May also play a role in docking of synaptic vesicles at presynaptic active zones.
Subunit structure	Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with SNAP23 and SNAPIN. Interacts with VAMP2. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 By similarity. Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Interacts with STXBP6. Ref.2
Subcellular location	Cell membrane; Single-pass type IV membrane protein Potential.
Tissue specificity	Expressed in all tissues tested inluding adipose, brain, testis, intestine, liver, heart, spleen, skeletal muscle and kidney. Ref.1 Ref.4
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular membrane fusion Inferred from direct assay PubMed 19109692. Source: RGD intracellular protein transport Inferred from electronic annotation. Source: InterPro intracellular transport Non-traceable author statement Ref.1. Source: RGD protein complex assembly Inferred from direct assay PubMed 10051443. Source: RGD synaptic vesicle docking involved in exocytosis Inferred from direct assay Ref.1. Source: RGD
Cellular_component	SNARE complex Inferred from direct assay Ref.3. Source: UniProtKB apical plasma membrane Inferred from direct assay PubMed 14759605. Source: RGD basolateral plasma membrane Inferred from direct assay PubMed 14759605. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW sarcolemma Inferred from direct assay PubMed 12388133. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Syntaxin-4		PRO_0000210204
Regions
	Topological domain	1 – 274	274	Cytoplasmic Potential
	Transmembrane	275 – 295	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	296 – 298	3	Extracellular Potential
	Domain	200 – 262	63	t-SNARE coiled-coil homology
	Region	154 – 298	145	Interaction with CENPF By similarity
	Coiled coil	38 – 163	126	Potential
Amino acid modifications
	Modified residue	15	1	Phosphoserine By similarity
	Modified residue	29	1	Phosphoserine By similarity
	Modified residue	117	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08850 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9E854270DFB3CB96	FASTA	298	34,209
	10 20 30 40 50 60 MRDRTHELRQ GDNISDDEDE VRVALVVHSG AARLSSPDDE FFQKVQTIRQ TMAKLESKVR 70 80 90 100 110 120 ELEKQQVTIL ATPLPEESMK QGLQNLREEI KQLGREVRAQ LKAIEPQKEE ADENYNSVNT 130 140 150 160 170 180 RMKKTQHGVL SQQFVELINK CNSMQSEYRE KNVERIRRQL KITNAGMVSD EELEQMLDSG 190 200 210 220 230 240 QSEVFVSNIL KDTQVTRQAL NEISARHSEI QQLERTIREL HEIFTFLATE VEMQGEMINR 250 260 270 280 290 IEKNILSSAD YVERGQEHVK IALENQKKAR KKKVMIAICV SVTVLILAVI IGITITVG

References

[1]	"The syntaxin family of vesicular transport receptors." Bennett M.K., Garcia-Arraras J.E., Elferink L.A., Peterson K.E., Fleming A.M., Hazuka C.D., Scheller R.H. Cell 74:863-873(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly." Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H. J. Biol. Chem. 277:28271-28279(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH STXBP6.
[3]	"Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis." Rao S.K., Huynh C., Proux-Gillardeaux V., Galli T., Andrews N.W. J. Biol. Chem. 279:20471-20479(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SNARE COMPLEX CHARACTERIZATION.
[4]	"Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport." Pooley R.D., Moynihan K.L., Soukoulis V., Reddy S., Francis R., Lo C., Ma L.-J., Bader D.M. J. Cell Sci. 121:3413-3421(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20821 mRNA. Translation: AAA03046.1.
IPI	IPI00204739.
PIR	E48213.
RefSeq	NP_112387.1. NM_031125.1.
UniGene	Rn.33218.
3D structure databases
ProteinModelPortal	Q08850.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-373N.
IntAct	Q08850. 4 interactions.
MINT	MINT-1509132.
STRING	10116.ENSRNOP00000026224.
PTM databases
PhosphoSite	Q08850.
Proteomic databases
PaxDb	Q08850.
PRIDE	Q08850.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81803.
KEGG	rno:81803.
UCSC	RGD:621019. rat.
Organism-specific databases
CTD	6810.
RGD	621019. Stx4.
Phylogenomic databases
eggNOG	COG5074.
HOGENOM	HOG000286023.
HOVERGEN	HBG000497.
InParanoid	Q08850.
KO	K13502.
OrthoDB	EOG4Z62PD.
Gene expression databases
Genevestigator	Q08850.
GermOnline	ENSRNOG00000019302. Rattus norvegicus.
Family and domain databases
InterPro	IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
Pfam	PF05739. SNARE. 1 hit. PF00804. Syntaxin. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615659.

Entry information

Entry name

STX4_RAT

Accession

Primary (citable) accession number: Q08850

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program