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Protein

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Gene

Mgat5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-RNO-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.155)
Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name:
GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V
Gene namesi
Name:Mgat5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620100. Mgat5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3017Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini31 – 740710LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1795132.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase APRO_0000080524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence analysis
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence analysis
Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ08834.
PRIDEiQ08834.

PTM databases

iPTMnetiQ08834.
PhosphoSiteiQ08834.

Expressioni

Gene expression databases

ExpressionAtlasiQ08834. baseline.
GenevisibleiQ08834. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004995.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 18 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ08834.
KOiK00744.
OMAiENWCPRL.
OrthoDBiEOG71K629.
PhylomeDBiQ08834.
TreeFamiTF313714.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI
60 70 80 90 100
LDLSKRYIKA LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL
110 120 130 140 150
ESKVDNLVNG TGANSTNSTT AVPSLVSLEK INVADIINGV QEKCVLPPMD
160 170 180 190 200
GYPHCEGKIK WMKDMWRSDP CYADYGVDGT SCSFFIYLSE VENWCPRLPW
210 220 230 240 250
RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR IRRMADAWIQ
260 270 280 290 300
AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW
310 320 330 340 350
SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI
360 370 380 390 400
DIVGLAQFKK TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW
410 420 430 440 450
GKWNLNPQQF YTMFPHTPDN SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV
460 470 480 490 500
YGKVDSFWKN KKIYLDIIHT YMEVHATVYG SSTKNIPSYV KNHGILSGRD
510 520 530 540 550
LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP PKSSKNTDFF
560 570 580 590 600
IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP
610 620 630 640 650
YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ
660 670 680 690 700
VCQESQLICE PSFFQHLNKE KDLLKYKVIC QSSELYKDIL VPSFYPKSKH
710 720 730 740
CVFQGDLLLF SCAGAHPTHQ RICPCRDFIK GQVALCKDCL
Length:740
Mass (Da):84,562
Last modified:February 1, 1995 - v1
Checksum:iC0BD7F820FEA959C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14284 mRNA. Translation: AAA41665.1.
PIRiA47134.
RefSeqiNP_075583.1. NM_023095.1.
UniGeneiRn.53998.

Genome annotation databases

EnsembliENSRNOT00000004995; ENSRNOP00000004995; ENSRNOG00000003614.
GeneIDi65271.
KEGGirno:65271.
UCSCiRGD:620100. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14284 mRNA. Translation: AAA41665.1.
PIRiA47134.
RefSeqiNP_075583.1. NM_023095.1.
UniGeneiRn.53998.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004995.

Chemistry

ChEMBLiCHEMBL1795132.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

PTM databases

iPTMnetiQ08834.
PhosphoSiteiQ08834.

Proteomic databases

PaxDbiQ08834.
PRIDEiQ08834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004995; ENSRNOP00000004995; ENSRNOG00000003614.
GeneIDi65271.
KEGGirno:65271.
UCSCiRGD:620100. rat.

Organism-specific databases

CTDi4249.
RGDi620100. Mgat5.

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ08834.
KOiK00744.
OMAiENWCPRL.
OrthoDBiEOG71K629.
PhylomeDBiQ08834.
TreeFamiTF313714.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-RNO-975577. N-Glycan antennae elongation.

Miscellaneous databases

PROiQ08834.

Gene expression databases

ExpressionAtlasiQ08834. baseline.
GenevisibleiQ08834. RN.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and expression of a cDNA encoding N-acetylglucosaminyltransferase V."
    Shoreibah M., Perng G.-S., Adler B., Weinstein J., Basu R., Cupples R., Wen D., Browne J.K., Buckhaults P., Fregien N., Pierce M.
    J. Biol. Chem. 268:15381-15385(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 375-386; 546-557 AND 592-607.
    Tissue: Kidney.
  2. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMGT5A_RAT
AccessioniPrimary (citable) accession number: Q08834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.