Q08834 (MGT5A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
EC=2.4.1.155

Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name=GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V

Gene names

Name:

Mgat5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 18 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A		PRO_0000080524
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 30	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 740	710	Lumenal Potential
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	432	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q08834 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C0BD7F820FEA959C	FASTA	740	84,562
	10 20 30 40 50 60 MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA 70 80 90 100 110 120 LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVNG TGANSTNSTT 130 140 150 160 170 180 AVPSLVSLEK INVADIINGV QEKCVLPPMD GYPHCEGKIK WMKDMWRSDP CYADYGVDGT 190 200 210 220 230 240 SCSFFIYLSE VENWCPRLPW RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR 250 260 270 280 290 300 IRRMADAWIQ AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW 310 320 330 340 350 360 SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI DIVGLAQFKK 370 380 390 400 410 420 TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW GKWNLNPQQF YTMFPHTPDN 430 440 450 460 470 480 SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV YGKVDSFWKN KKIYLDIIHT YMEVHATVYG 490 500 510 520 530 540 SSTKNIPSYV KNHGILSGRD LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP 550 560 570 580 590 600 PKSSKNTDFF IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP 610 620 630 640 650 660 YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ VCQESQLICE 670 680 690 700 710 720 PSFFQHLNKE KDLLKYKVIC QSSELYKDIL VPSFYPKSKH CVFQGDLLLF SCAGAHPTHQ 730 740 RICPCRDFIK GQVALCKDCL

References

[1]

"Isolation, characterization, and expression of a cDNA encoding N-acetylglucosaminyltransferase V." Shoreibah M., Perng G.-S., Adler B., Weinstein J., Basu R., Cupples R., Wen D., Browne J.K., Buckhaults P., Fregien N., Pierce M. J. Biol. Chem. 268:15381-15385(1993) [PubMed: 8340368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 375-386; 546-557 AND 592-607. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14284 mRNA. Translation: AAA41665.1.
IPI	IPI00204664.
PIR	A47134.
RefSeq	NP_075583.1. NM_023095.1.
UniGene	Rn.53998.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q08834.
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
PTM databases
PhosphoSite	Q08834.
Proteomic databases
PRIDE	Q08834.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004995; ENSRNOP00000004995; ENSRNOG00000003614.
GeneID	65271.
KEGG	rno:65271.
UCSC	NM_023095. rat.
Organism-specific databases
CTD	4249.
RGD	620100. Mgat5.
Phylogenomic databases
eggNOG	roNOG10715.
GeneTree	ENSGT00390000012263.
HOVERGEN	HBG052469.
InParanoid	Q08834.
OMA	ENWCPRL.
OrthoDB	EOG4HT8RM.
PhylomeDB	Q08834.
Gene expression databases
ArrayExpress	Q08834.
Genevestigator	Q08834.
GermOnline	ENSRNOG00000003614. Rattus norvegicus.
Family and domain databases
InterPro	IPR024144. Glyco_trans_18. [Graphical view]
KO	K00744.
PANTHER	PTHR15075. PTHR15075. 1 hit.
ProtoNet	Search...
Other
NextBio	614221.

Entry information

Entry name

MGT5A_RAT

Accession

Primary (citable) accession number: Q08834

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program