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Protein

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Gene

Mgat5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->4))-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->4))-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-RNO-975577 N-Glycan antennae elongation
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT18 Glycosyltransferase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.155)
Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name:
GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V
Gene namesi
Name:Mgat5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620100 Mgat5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini31 – 740LumenalSequence analysisAdd BLAST710

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795132

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805241 – 740Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase AAdd BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi333N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi432N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi446N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ08834
PRIDEiQ08834

PTM databases

iPTMnetiQ08834
PhosphoSitePlusiQ08834

Expressioni

Gene expression databases

BgeeiENSRNOG00000003614
ExpressionAtlasiQ08834 baseline and differential
GenevisibleiQ08834 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004995

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 18 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDYS Eukaryota
ENOG410XTV7 LUCA
GeneTreeiENSGT00390000012263
HOGENOMiHOG000006557
HOVERGENiHBG052469
InParanoidiQ08834
KOiK00744
OMAiGKVDNFW
OrthoDBiEOG091G044F
PhylomeDBiQ08834
TreeFamiTF313714

Family and domain databases

InterProiView protein in InterPro
IPR027833 DUF4525
IPR026116 GlyclTrfase_18
PANTHERiPTHR15075 PTHR15075, 1 hit
PfamiView protein in Pfam
PF15027 DUF4525, 1 hit

Sequencei

Sequence statusi: Complete.

Q08834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI
60 70 80 90 100
LDLSKRYIKA LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL
110 120 130 140 150
ESKVDNLVNG TGANSTNSTT AVPSLVSLEK INVADIINGV QEKCVLPPMD
160 170 180 190 200
GYPHCEGKIK WMKDMWRSDP CYADYGVDGT SCSFFIYLSE VENWCPRLPW
210 220 230 240 250
RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR IRRMADAWIQ
260 270 280 290 300
AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW
310 320 330 340 350
SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI
360 370 380 390 400
DIVGLAQFKK TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW
410 420 430 440 450
GKWNLNPQQF YTMFPHTPDN SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV
460 470 480 490 500
YGKVDSFWKN KKIYLDIIHT YMEVHATVYG SSTKNIPSYV KNHGILSGRD
510 520 530 540 550
LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP PKSSKNTDFF
560 570 580 590 600
IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP
610 620 630 640 650
YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ
660 670 680 690 700
VCQESQLICE PSFFQHLNKE KDLLKYKVIC QSSELYKDIL VPSFYPKSKH
710 720 730 740
CVFQGDLLLF SCAGAHPTHQ RICPCRDFIK GQVALCKDCL
Length:740
Mass (Da):84,562
Last modified:February 1, 1995 - v1
Checksum:iC0BD7F820FEA959C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14284 mRNA Translation: AAA41665.1
PIRiA47134
RefSeqiNP_075583.1, NM_023095.1
XP_008767721.2, XM_008769499.2
XP_017454423.1, XM_017598934.1
UniGeneiRn.53998

Genome annotation databases

EnsembliENSRNOT00000004995; ENSRNOP00000004995; ENSRNOG00000003614
GeneIDi65271
KEGGirno:65271
UCSCiRGD:620100 rat

Similar proteinsi

Entry informationi

Entry nameiMGT5A_RAT
AccessioniPrimary (citable) accession number: Q08834
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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