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Q08826 (SNX3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sorting nexin-3
Alternative name(s):
Golgi retention deficient protein 19
Gene names
Name:SNX3
Synonyms:GRD19
Ordered Locus Names:YOR357C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length162 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Ref.1

Subunit structure

Monomer. Interacts with RBD2, YIF1, YIP1 and YIP5. Ref.4 Ref.7

Subcellular location

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Potential. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side Potential. Note: Mainly cytoplasmic. A minor amount associates with membranes. Ref.1

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 PX (phox homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 162162Sorting nexin-3
PRO_0000213805

Regions

Domain38 – 161124PX

Sites

Binding site811Phosphatidylinositol 3-phosphate
Binding site831Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen
Binding site1121Phosphatidylinositol 3-phosphate
Binding site1271Phosphatidylinositol 3-phosphate

Secondary structure

.................. 162
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q08826 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0633831F6CF165FC

FASTA16218,770
        10         20         30         40         50         60 
MPREFKSFGS TEKSLLSKGH GEPSYSEIYA EPENFLEIEV HNPKTHIPNG MDSKGMFTDY 

        70         80         90        100        110        120 
EIICRTNLPS FHKRVSKVRR RYSDFEFFRK CLIKEISMLN HPKVMVPHLP GKILLSNRFS 

       130        140        150        160 
NEVIEERRQG LNTWMQSVAG HPLLQSGSKV LVRFIEAEKF VG 

« Hide

References

« Hide 'large scale' references
[1]"Retrieval of resident late-Golgi membrane proteins from the prevacuolar compartment of Saccharomyces cerevisiae is dependent on the function of Grd19p."
Voos W., Stevens T.H.
J. Cell Biol. 140:577-590(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The phox homology (PX) domain protein interaction network in yeast."
Vollert C.S., Uetz P.
Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RBD2; YIF1; YIP1 AND YIP5.
[5]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate."
Zhou C.-Z., de La Sierra-Gallay I.L., Quevillon-Cheruel S., Collinet B., Minard P., Blondeau K., Henckes G., Aufrere R., Leulliot N., Graille M., Sorel I., Savarin P., de la Torre F., Poupon A., Janin J., van Tilbeurgh H.
J. Biol. Chem. 278:50371-50376(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) IN COMPLEX WITH PHOSPHATIDYLINOSITOL 3-PHOSPHATE, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF016101 Genomic DNA. Translation: AAC15913.1.
Z75265 Genomic DNA. Translation: CAA99686.1.
BK006948 Genomic DNA. Translation: DAA11118.1.
PIRS67269.
RefSeqNP_015002.3. NM_001183777.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OCSX-ray2.03A1-162[»]
1OCUX-ray2.30A/B1-162[»]
DisProtDP00482.
ProteinModelPortalQ08826.
SMRQ08826. Positions 28-161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34742. 37 interactions.
IntActQ08826. 15 interactions.
MINTMINT-3976730.
STRING4932.YOR357C.

Proteomic databases

PaxDbQ08826.
PeptideAtlasQ08826.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR357C; YOR357C; YOR357C.
GeneID854539.
KEGGsce:YOR357C.

Organism-specific databases

CYGDYOR357c.
SGDS000005884. SNX3.

Phylogenomic databases

eggNOGCOG5391.
GeneTreeENSGT00650000093174.
HOGENOMHOG000234882.
KOK17918.
OMANPITHGV.
OrthoDBEOG7B320H.

Enzyme and pathway databases

BioCycYEAST:G3O-33828-MONOMER.

Gene expression databases

GenevestigatorQ08826.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR001683. Phox.
[Graphical view]
PfamPF00787. PX. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ08826.
NextBio976940.
PROQ08826.

Entry information

Entry nameSNX3_YEAST
AccessionPrimary (citable) accession number: Q08826
Secondary accession number(s): D6W352
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references