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Q08826

- SNX3_YEAST

UniProt

Q08826 - SNX3_YEAST

Protein

Sorting nexin-3

Gene

SNX3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811Phosphatidylinositol 3-phosphate1 Publication
    Binding sitei83 – 831Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen1 Publication
    Binding sitei112 – 1121Phosphatidylinositol 3-phosphate1 Publication
    Binding sitei127 – 1271Phosphatidylinositol 3-phosphate1 Publication

    GO - Molecular functioni

    1. phosphatidylinositol-3-phosphate binding Source: SGD

    GO - Biological processi

    1. late endosome to Golgi transport Source: SGD
    2. protein localization Source: SGD
    3. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    Lipid-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33828-MONOMER.
    ReactomeiREACT_188772. WNT ligand biogenesis and trafficking.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sorting nexin-3
    Alternative name(s):
    Golgi retention deficient protein 19
    Gene namesi
    Name:SNX3
    Synonyms:GRD19
    Ordered Locus Names:YOR357C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR357c.
    SGDiS000005884. SNX3.

    Subcellular locationi

    Cytoplasm 1 Publication. Golgi apparatus membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated. Prevacuolar compartment membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
    Note: Mainly cytoplasmic. A minor amount associates with membranes.

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. endosome Source: SGD
    3. Golgi membrane Source: UniProtKB-SubCell
    4. late endosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 162162Sorting nexin-3PRO_0000213805Add
    BLAST

    Proteomic databases

    MaxQBiQ08826.
    PaxDbiQ08826.
    PeptideAtlasiQ08826.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08826.

    Interactioni

    Subunit structurei

    Monomer. Interacts with RBD2, YIF1, YIP1 and YIP5.2 Publications

    Protein-protein interaction databases

    BioGridi34742. 37 interactions.
    IntActiQ08826. 15 interactions.
    MINTiMINT-3976730.
    STRINGi4932.YOR357C.

    Structurei

    Secondary structure

    1
    162
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 4611
    Beta strandi50 – 534
    Beta strandi57 – 6610
    Beta strandi74 – 818
    Helixi82 – 9817
    Helixi117 – 1193
    Helixi121 – 13919
    Helixi142 – 1476
    Helixi149 – 1568

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OCSX-ray2.03A1-162[»]
    1OCUX-ray2.30A/B1-162[»]
    DisProtiDP00482.
    ProteinModelPortaliQ08826.
    SMRiQ08826. Positions 28-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ08826.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 161124PXPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the sorting nexin family.Curated
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5391.
    GeneTreeiENSGT00650000093174.
    HOGENOMiHOG000234882.
    KOiK17918.
    OMAiNPITHGV.
    OrthoDBiEOG7B320H.

    Family and domain databases

    Gene3Di3.30.1520.10. 1 hit.
    InterProiIPR001683. Phox.
    [Graphical view]
    PfamiPF00787. PX. 1 hit.
    [Graphical view]
    SMARTiSM00312. PX. 1 hit.
    [Graphical view]
    SUPFAMiSSF64268. SSF64268. 1 hit.
    PROSITEiPS50195. PX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08826-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPREFKSFGS TEKSLLSKGH GEPSYSEIYA EPENFLEIEV HNPKTHIPNG    50
    MDSKGMFTDY EIICRTNLPS FHKRVSKVRR RYSDFEFFRK CLIKEISMLN 100
    HPKVMVPHLP GKILLSNRFS NEVIEERRQG LNTWMQSVAG HPLLQSGSKV 150
    LVRFIEAEKF VG 162
    Length:162
    Mass (Da):18,770
    Last modified:November 1, 1996 - v1
    Checksum:i0633831F6CF165FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF016101 Genomic DNA. Translation: AAC15913.1.
    Z75265 Genomic DNA. Translation: CAA99686.1.
    BK006948 Genomic DNA. Translation: DAA11118.1.
    PIRiS67269.
    RefSeqiNP_015002.3. NM_001183777.3.

    Genome annotation databases

    EnsemblFungiiYOR357C; YOR357C; YOR357C.
    GeneIDi854539.
    KEGGisce:YOR357C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF016101 Genomic DNA. Translation: AAC15913.1 .
    Z75265 Genomic DNA. Translation: CAA99686.1 .
    BK006948 Genomic DNA. Translation: DAA11118.1 .
    PIRi S67269.
    RefSeqi NP_015002.3. NM_001183777.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OCS X-ray 2.03 A 1-162 [» ]
    1OCU X-ray 2.30 A/B 1-162 [» ]
    DisProti DP00482.
    ProteinModelPortali Q08826.
    SMRi Q08826. Positions 28-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34742. 37 interactions.
    IntActi Q08826. 15 interactions.
    MINTi MINT-3976730.
    STRINGi 4932.YOR357C.

    Proteomic databases

    MaxQBi Q08826.
    PaxDbi Q08826.
    PeptideAtlasi Q08826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR357C ; YOR357C ; YOR357C .
    GeneIDi 854539.
    KEGGi sce:YOR357C.

    Organism-specific databases

    CYGDi YOR357c.
    SGDi S000005884. SNX3.

    Phylogenomic databases

    eggNOGi COG5391.
    GeneTreei ENSGT00650000093174.
    HOGENOMi HOG000234882.
    KOi K17918.
    OMAi NPITHGV.
    OrthoDBi EOG7B320H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33828-MONOMER.
    Reactomei REACT_188772. WNT ligand biogenesis and trafficking.

    Miscellaneous databases

    EvolutionaryTracei Q08826.
    NextBioi 976940.
    PROi Q08826.

    Gene expression databases

    Genevestigatori Q08826.

    Family and domain databases

    Gene3Di 3.30.1520.10. 1 hit.
    InterProi IPR001683. Phox.
    [Graphical view ]
    Pfami PF00787. PX. 1 hit.
    [Graphical view ]
    SMARTi SM00312. PX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64268. SSF64268. 1 hit.
    PROSITEi PS50195. PX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Retrieval of resident late-Golgi membrane proteins from the prevacuolar compartment of Saccharomyces cerevisiae is dependent on the function of Grd19p."
      Voos W., Stevens T.H.
      J. Cell Biol. 140:577-590(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The phox homology (PX) domain protein interaction network in yeast."
      Vollert C.S., Uetz P.
      Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RBD2; YIF1; YIP1 AND YIP5.
    5. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) IN COMPLEX WITH PHOSPHATIDYLINOSITOL 3-PHOSPHATE, SUBUNIT.

    Entry informationi

    Entry nameiSNX3_YEAST
    AccessioniPrimary (citable) accession number: Q08826
    Secondary accession number(s): D6W352
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3