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Protein

Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial

Gene

CIR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts electrons from ETF and reduces ubiquinone.By similarity

Catalytic activityi

Reduced electron-transferring flavoprotein + ubiquinone = electron-transferring flavoprotein + ubiquinol.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi574 – 5741Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi600 – 6001Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi603 – 6031Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi606 – 6061Iron-sulfur (4Fe-4S)Sequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 7915FADSequence analysisAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Ubiquinone

Enzyme and pathway databases

BioCyciYEAST:G3O-33827-MONOMER.
ReactomeiR-SCE-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (EC:1.5.5.1)
Short name:
ETF-QO
Short name:
ETF-ubiquinone oxidoreductase
Alternative name(s):
Changed intracellular redox state protein 2
Electron-transferring-flavoprotein dehydrogenase
Short name:
ETF dehydrogenase
Gene namesi
Name:CIR2
Ordered Locus Names:YOR356W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR356W.
SGDiS000005883. CIR2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Displays higher growth rate and higher sensitivity to superoxide and heat stress, on nonfermentable carbon sources. Leads to decreased intracellular oxidation upon heat shock.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 631Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrialPRO_0000008667
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ08822.
PeptideAtlasiQ08822.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ECM4P361561EBI-6705,EBI-2042717

Protein-protein interaction databases

BioGridi34741. 8 interactions.
DIPiDIP-6685N.
IntActiQ08822. 2 interactions.
MINTiMINT-617086.

Structurei

3D structure databases

ProteinModelPortaliQ08822.
SMRiQ08822. Positions 52-628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini591 – 620304Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ETF-QO/FixC family.Curated
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000010773.
HOGENOMiHOG000259450.
InParanoidiQ08822.
KOiK00311.
OMAiLRGMEPW.
OrthoDBiEOG71CFVN.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR007859. ETFD_OxRdtase/FixX.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PANTHERiPTHR10617:SF107. PTHR10617:SF107. 2 hits.
PfamiPF05187. ETF_QO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKFTNENLI RGIRMTISAK SRHLALGTDM TRKFSLSCRF LNKANLTEEE
60 70 80 90 100
KELLNEPRAR DYVDVCIVGG GPAGLATAIK LKQLDNSSGT GQLRVVVLEK
110 120 130 140 150
SSVLGGQTVS GAILEPGVWK ELFPDEKSDI GIPLPKELAT LVTKEHLKFL
160 170 180 190 200
KGKWAISVPE PSQMINKGRN YIVSLNQVVG YLGEKAEEVG VEVYPGIAVS
210 220 230 240 250
DLIYDENNAV KGVITKDAGI SKSGKPKETF ERGMEFWARQ TVLAEGCHGS
260 270 280 290 300
LTKQALAKYD LRKGRQHQTY GLGIKEVWEV KPENFNKGFA AHTMGYPLTN
310 320 330 340 350
DVYGGGFQYH FGDGLVTVGL VVGLDYKNPY VSPYKEFQKM KHHPYYSKVL
360 370 380 390 400
EGGKCIAYAA RALNEGGLQS VPKLNFPGGV LVGASAGFMN VPKIKGTHTA
410 420 430 440 450
MKSGLLAAES IFESIKGLPV LEEVEDEDAK MAMFDKEATI NLESYESAFK
460 470 480 490 500
ESSIYKELYE VRNIRPSFSG KLGGYGGMIY SGIDSLILKG KVPWTLKFDE
510 520 530 540 550
KNDGEILEPA SKYKPIEYPK PDGVISFDIL TSVSRTGTYH DDDEPCHLRV
560 570 580 590 600
PGQDMVKYAE RSFPVWKGVE SRFCPAGVYE FVKDEKSPVG TRLQINSQNC
610 620 630
IHCKTCDIKA PRQDITWKVP EGGDGPKYTL T
Length:631
Mass (Da):69,634
Last modified:November 1, 1997 - v1
Checksum:i7493F67093D88391
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75264 Genomic DNA. Translation: CAA99685.1.
BK006948 Genomic DNA. Translation: DAA11117.1.
PIRiS67268.
RefSeqiNP_015001.1. NM_001183776.1.

Genome annotation databases

EnsemblFungiiYOR356W; YOR356W; YOR356W.
GeneIDi854538.
KEGGisce:YOR356W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75264 Genomic DNA. Translation: CAA99685.1.
BK006948 Genomic DNA. Translation: DAA11117.1.
PIRiS67268.
RefSeqiNP_015001.1. NM_001183776.1.

3D structure databases

ProteinModelPortaliQ08822.
SMRiQ08822. Positions 52-628.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34741. 8 interactions.
DIPiDIP-6685N.
IntActiQ08822. 2 interactions.
MINTiMINT-617086.

Proteomic databases

MaxQBiQ08822.
PeptideAtlasiQ08822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR356W; YOR356W; YOR356W.
GeneIDi854538.
KEGGisce:YOR356W.

Organism-specific databases

EuPathDBiFungiDB:YOR356W.
SGDiS000005883. CIR2.

Phylogenomic databases

GeneTreeiENSGT00390000010773.
HOGENOMiHOG000259450.
InParanoidiQ08822.
KOiK00311.
OMAiLRGMEPW.
OrthoDBiEOG71CFVN.

Enzyme and pathway databases

BioCyciYEAST:G3O-33827-MONOMER.
ReactomeiR-SCE-611105. Respiratory electron transport.

Miscellaneous databases

NextBioi976937.
PROiQ08822.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR007859. ETFD_OxRdtase/FixX.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PANTHERiPTHR10617:SF107. PTHR10617:SF107. 2 hits.
PfamiPF05187. ETF_QO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  7. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "The Saccharomyces cerevisiae genes, AIM45, YGR207c/CIR1 and YOR356w/CIR2, are involved in cellular redox state under stress conditions."
    Lopes J., Pinto M.J., Rodrigues A., Vasconcelos F., Oliveira R.
    Open Microbiol. J. 4:75-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiETFD_YEAST
AccessioniPrimary (citable) accession number: Q08822
Secondary accession number(s): D6W351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.