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Protein

Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial

Gene

CIR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts electrons from ETF and reduces ubiquinone.By similarity

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Reduced electron-transferring flavoprotein + ubiquinone = electron-transferring flavoprotein + ubiquinol.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi574Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi600Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi603Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi606Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 79FADSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
Ligand4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Ubiquinone

Enzyme and pathway databases

BioCyciYEAST:G3O-33827-MONOMER
ReactomeiR-SCE-611105 Respiratory electron transport

Names & Taxonomyi

Protein namesi
Recommended name:
Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (EC:1.5.5.1)
Short name:
ETF-QO
Short name:
ETF-ubiquinone oxidoreductase
Alternative name(s):
Changed intracellular redox state protein 2
Electron-transferring-flavoprotein dehydrogenase
Short name:
ETF dehydrogenase
Gene namesi
Name:CIR2
Ordered Locus Names:YOR356W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR356W
SGDiS000005883 CIR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Displays higher growth rate and higher sensitivity to superoxide and heat stress, on nonfermentable carbon sources. Leads to decreased intracellular oxidation upon heat shock.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000008667? – 631Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ08822
PaxDbiQ08822
PRIDEiQ08822

Interactioni

Protein-protein interaction databases

BioGridi34741, 67 interactors
DIPiDIP-6685N
IntActiQ08822, 6 interactors
STRINGi4932.YOR356W

Structurei

3D structure databases

ProteinModelPortaliQ08822
SMRiQ08822
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini591 – 6204Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the ETF-QO/FixC family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000010773
HOGENOMiHOG000259450
InParanoidiQ08822
KOiK00311
OMAiVPRITTH
OrthoDBiEOG092C0UBS

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR036188 FAD/NAD-bd_sf
SUPFAMiSSF51905 SSF51905, 2 hits
PROSITEiView protein in PROSITE
PS51379 4FE4S_FER_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKFTNENLI RGIRMTISAK SRHLALGTDM TRKFSLSCRF LNKANLTEEE
60 70 80 90 100
KELLNEPRAR DYVDVCIVGG GPAGLATAIK LKQLDNSSGT GQLRVVVLEK
110 120 130 140 150
SSVLGGQTVS GAILEPGVWK ELFPDEKSDI GIPLPKELAT LVTKEHLKFL
160 170 180 190 200
KGKWAISVPE PSQMINKGRN YIVSLNQVVG YLGEKAEEVG VEVYPGIAVS
210 220 230 240 250
DLIYDENNAV KGVITKDAGI SKSGKPKETF ERGMEFWARQ TVLAEGCHGS
260 270 280 290 300
LTKQALAKYD LRKGRQHQTY GLGIKEVWEV KPENFNKGFA AHTMGYPLTN
310 320 330 340 350
DVYGGGFQYH FGDGLVTVGL VVGLDYKNPY VSPYKEFQKM KHHPYYSKVL
360 370 380 390 400
EGGKCIAYAA RALNEGGLQS VPKLNFPGGV LVGASAGFMN VPKIKGTHTA
410 420 430 440 450
MKSGLLAAES IFESIKGLPV LEEVEDEDAK MAMFDKEATI NLESYESAFK
460 470 480 490 500
ESSIYKELYE VRNIRPSFSG KLGGYGGMIY SGIDSLILKG KVPWTLKFDE
510 520 530 540 550
KNDGEILEPA SKYKPIEYPK PDGVISFDIL TSVSRTGTYH DDDEPCHLRV
560 570 580 590 600
PGQDMVKYAE RSFPVWKGVE SRFCPAGVYE FVKDEKSPVG TRLQINSQNC
610 620 630
IHCKTCDIKA PRQDITWKVP EGGDGPKYTL T
Length:631
Mass (Da):69,634
Last modified:November 1, 1997 - v1
Checksum:i7493F67093D88391
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75264 Genomic DNA Translation: CAA99685.1
BK006948 Genomic DNA Translation: DAA11117.1
PIRiS67268
RefSeqiNP_015001.1, NM_001183776.1

Genome annotation databases

EnsemblFungiiYOR356W; YOR356W; YOR356W
GeneIDi854538
KEGGisce:YOR356W

Similar proteinsi

Entry informationi

Entry nameiETFD_YEAST
AccessioniPrimary (citable) accession number: Q08822
Secondary accession number(s): D6W351
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health