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Q087T3 (NRFA_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome c-552
EC=1.7.2.2
Alternative name(s):
Ammonia-forming cytochrome c nitrite reductase
Short name=Cytochrome c nitrite reductase
Gene names
Name:nrfA
Ordered Locus Names:Sfri_0622
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity. HAMAP MF_01182

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity. HAMAP MF_01182

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity HAMAP MF_01182.

Sequence similarities

Belongs to the cytochrome c-552 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 463440Cytochrome c-552 HAMAP MF_01182
PRO_0000268974

Sites

Metal binding831Iron (heme 3 axial ligand) By similarity
Metal binding1151Iron (heme 1 axial ligand) By similarity
Metal binding1531Iron (heme 2 axial ligand) By similarity
Metal binding1951Iron (heme 3 axial ligand) By similarity
Metal binding1971Calcium By similarity
Metal binding1981Calcium; via carbonyl oxygen By similarity
Metal binding2461Calcium; via carbonyl oxygen By similarity
Metal binding2481Calcium By similarity
Metal binding2601Iron (heme 5 axial ligand) By similarity
Metal binding2711Iron (heme 4 axial ligand) By similarity
Metal binding2861Iron (heme 2 axial ligand) By similarity
Metal binding3031Iron (heme 5 axial ligand) By similarity
Metal binding3781Iron (heme 4 axial ligand) By similarity
Binding site1111Heme 1 (covalent) By similarity
Binding site1141Heme 1 (covalent) By similarity
Binding site1491Heme 2 (covalent) By similarity
Binding site1521Heme 2 (covalent) By similarity
Binding site1911Heme 3 (covalent) By similarity
Binding site1941Heme 3 (covalent) By similarity
Binding site1981Substrate By similarity
Binding site2491Substrate By similarity
Binding site2671Heme 4 (covalent) By similarity
Binding site2701Heme 4 (covalent) By similarity
Binding site2991Heme 5 (covalent) By similarity
Binding site3021Heme 5 (covalent) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q087T3 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: 64A37A548C5FF050

FASTA46351,800
        10         20         30         40         50         60 
MNVKSIALSA VIATSFLAAG AMASEKTEPR NEVYKDKFSK QYDSWHATDE SKEVVDMLEK 

        70         80         90        100        110        120 
VPSLVVLWAG YGFAKDYNAP RGHMYAVTDV TNTLRTGAPK NAEDGPMPMA CWSCKSPDVP 

       130        140        150        160        170        180 
RVIEEQGEDG YFSGKWAKGG PEIVNVLGCA DCHEKGSSKL RMSRPFAERA MTTLNTPFDK 

       190        200        210        220        230        240 
ASRKDKQSMV CAQCHVEYYF EKTAERPGFV KFPWDMGTTV EQMEVYYDSM EFSDWTHAVS 

       250        260        270        280        290        300 
KTPMLKAQHP GYETWKLGVH GQNNVSCVDC HMPKVTNDKG RKFTDHKVGN PFDRFEETCA 

       310        320        330        340        350        360 
TCHSQSKEFM VKQVEESKQK AQDLKARVET QLVKAHFEAK AAWDAGATEV EMKPILMDIR 

       370        380        390        400        410        420 
HSQWRWDYAT ASHGASSHAP AEVLRILGTS LDKAADARVK LAQLLGAKGV KQPIAYPDTS 

       430        440        450        460 
TKAKAQAALG MNMKTMNAEK AEFKKTLVPK WKEEAKKREA TYK

« Hide

References

[1]"Complete sequence of Shewanella frigidimarina NCIMB 400."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. expand/collapse author list , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCIMB 400.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000447 Genomic DNA. Translation: ABI70482.1.
RefSeqYP_749320.1. NC_008345.1.

3D structure databases

ProteinModelPortalQ087T3.
SMRQ087T3. Positions 28-457.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ087T3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4278422.
GenomeReviewsGene locus Sfri_0622 in contig CP000447_GR.
KEGGsfr:Sfri_0622.
NMPDRfig|318167.10.peg.592.
PATRIC23495037. VBISheFri14343_0643.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3303.
HOGENOMHBG488281.
OMACGQCHAS.
PhylomeDBQ087T3.
ProtClustDBPRK11125.

Family and domain databases

HAMAPMF_01182. Cytochrom_C552.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
KOK03385.
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. Cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNRFA_SHEFN
AccessionPrimary (citable) accession number: Q087T3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 31, 2006
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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