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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei625 – 6251ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-738-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Sfri_0705
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000684 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863Leucine--tRNA ligasePRO_0000334817Add
BLAST

Proteomic databases

PRIDEiQ087K2.

Interactioni

Protein-protein interaction databases

STRINGi318167.Sfri_0705.

Structurei

3D structure databases

ProteinModelPortaliQ087K2.
SMRiQ087K2. Positions 228-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q087K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQELYNPSEI EALVQKHWHE HKTFEVTEDE SKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVARYQRLQG KNVLQPIGWD SFGLPAENAA INNKASPAPW
110 120 130 140 150
TYENIDYMKN QLKLLGFGYD WSREIATCTP EYYRWEQWFF TQLYAKGLVY
160 170 180 190 200
KKTSSVNWCP NDETVLANEQ VQDGCCWRCD TEVIQKEIPQ WFIKITDYAE
210 220 230 240 250
ELLNDIDTLD GWPEQVKAMQ RNWIGRSEGI EMTFAVADSD ASFDIYTTRP
260 270 280 290 300
DTLMGVTYVA IAAGHPLAEQ SAQNNPELAA FLEECKNADT TEAAMASMEK
310 320 330 340 350
KGVATGLQAI HPISGKLVPI WVANFVLMNY GTGAVMSVPA HDQRDYEFAT
360 370 380 390 400
KYQLAIEAVI KPVDGEVDVS KEAYTEKGVV FNSGDAFPEL DGLDFQAAFE
410 420 430 440 450
AIDARLTAEG KGKRQVNYRL RDWGVSRQRY WGAPIPMVTL ADGTVVPTPE
460 470 480 490 500
DQLPVILPED VVMDGIQSPI KADKAWAETT VNGQPATRET DTFDTFMESS
510 520 530 540 550
WYYARYCSPH ADEMLDPAKA NYWLPVDQYI GGIEHACMHL LYFRFFHKLL
560 570 580 590 600
RDAGLVNSDE PAKQLLTQGM VLADAYYYTN EKGARVWVSP LEVTVVEKDD
610 620 630 640 650
KGRIIKAIDN QGHELVYTGM SKMSKSKNNG IDPQVMVEKY GADTVRLFMM
660 670 680 690 700
FASPPELTLE WQESGVEGAH RFIKRFWKLA SDHVAAGKTE ALDTSKLNAD
710 720 730 740 750
QKALRRELHK TIAKVSDDIA RRQMFNTAVA SVMELMNHLL KASQETAQDR
760 770 780 790 800
ALLAEALSAV TRLLYPIVPH MTFTLWNELG NEGDIEDSRW PEVDESALVE
810 820 830 840 850
DSKLIVVQVN GKVRAKITVA ADASKEDVEA LGMSDEHVQK HTEGLTVRKV
860
IYVPGKLLSI VAN
Length:863
Mass (Da):97,261
Last modified:May 20, 2008 - v2
Checksum:i1675C3830AD8D792
GO

Sequence cautioni

The sequence ABI70563.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA. Translation: ABI70563.1. Different initiation.
RefSeqiYP_749401.1. NC_008345.1.

Genome annotation databases

EnsemblBacteriaiABI70563; ABI70563; Sfri_0705.
KEGGisfr:Sfri_0705.
PATRICi23495227. VBISheFri14343_0729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA. Translation: ABI70563.1. Different initiation.
RefSeqiYP_749401.1. NC_008345.1.

3D structure databases

ProteinModelPortaliQ087K2.
SMRiQ087K2. Positions 228-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318167.Sfri_0705.

Proteomic databases

PRIDEiQ087K2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI70563; ABI70563; Sfri_0705.
KEGGisfr:Sfri_0705.
PATRICi23495227. VBISheFri14343_0729.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-738-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIMB 400.

Entry informationi

Entry nameiSYL_SHEFN
AccessioniPrimary (citable) accession number: Q087K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 24, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.