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Q087K2 (SYL_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Sfri_0705
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABI70563.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334817

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q087K2 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 1675C3830AD8D792

FASTA86397,261
        10         20         30         40         50         60 
MQELYNPSEI EALVQKHWHE HKTFEVTEDE SKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVARYQRLQG KNVLQPIGWD SFGLPAENAA INNKASPAPW TYENIDYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WSREIATCTP EYYRWEQWFF TQLYAKGLVY KKTSSVNWCP NDETVLANEQ VQDGCCWRCD 

       190        200        210        220        230        240 
TEVIQKEIPQ WFIKITDYAE ELLNDIDTLD GWPEQVKAMQ RNWIGRSEGI EMTFAVADSD 

       250        260        270        280        290        300 
ASFDIYTTRP DTLMGVTYVA IAAGHPLAEQ SAQNNPELAA FLEECKNADT TEAAMASMEK 

       310        320        330        340        350        360 
KGVATGLQAI HPISGKLVPI WVANFVLMNY GTGAVMSVPA HDQRDYEFAT KYQLAIEAVI 

       370        380        390        400        410        420 
KPVDGEVDVS KEAYTEKGVV FNSGDAFPEL DGLDFQAAFE AIDARLTAEG KGKRQVNYRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGAPIPMVTL ADGTVVPTPE DQLPVILPED VVMDGIQSPI KADKAWAETT 

       490        500        510        520        530        540 
VNGQPATRET DTFDTFMESS WYYARYCSPH ADEMLDPAKA NYWLPVDQYI GGIEHACMHL 

       550        560        570        580        590        600 
LYFRFFHKLL RDAGLVNSDE PAKQLLTQGM VLADAYYYTN EKGARVWVSP LEVTVVEKDD 

       610        620        630        640        650        660 
KGRIIKAIDN QGHELVYTGM SKMSKSKNNG IDPQVMVEKY GADTVRLFMM FASPPELTLE 

       670        680        690        700        710        720 
WQESGVEGAH RFIKRFWKLA SDHVAAGKTE ALDTSKLNAD QKALRRELHK TIAKVSDDIA 

       730        740        750        760        770        780 
RRQMFNTAVA SVMELMNHLL KASQETAQDR ALLAEALSAV TRLLYPIVPH MTFTLWNELG 

       790        800        810        820        830        840 
NEGDIEDSRW PEVDESALVE DSKLIVVQVN GKVRAKITVA ADASKEDVEA LGMSDEHVQK 

       850        860 
HTEGLTVRKV IYVPGKLLSI VAN 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000447 Genomic DNA. Translation: ABI70563.1. Different initiation.
RefSeqYP_749401.1. NC_008345.1.

3D structure databases

ProteinModelPortalQ087K2.
SMRQ087K2. Positions 228-416.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318167.Sfri_0705.

Proteomic databases

PRIDEQ087K2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI70563; ABI70563; Sfri_0705.
GeneID4278748.
KEGGsfr:Sfri_0705.
PATRIC23495227. VBISheFri14343_0729.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSFRI318167:GIXS-738-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SHEFN
AccessionPrimary (citable) accession number: Q087K2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries