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Protein

Riboflavin transporter MCH5

Gene

MCH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Riboflavin transporter involved in riboflavin (vitamin B2) uptake. Does not act in the transport of monocarboxylic acids across the plasma membrane.1 Publication

Kineticsi

  1. KM=17 µM for riboflavin1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    GO - Molecular functioni

    • riboflavin transporter activity Source: SGD

    GO - Biological processi

    • ion transmembrane transport Source: GO_Central
    • riboflavin transport Source: SGD
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33790-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.13.4. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin transporter MCH5
    Gene namesi
    Name:MCH5
    Ordered Locus Names:YOR306C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR306C.
    SGDiS000005833. MCH5.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 103CytoplasmicSequence analysisAdd BLAST103
    Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
    Topological domaini125 – 143ExtracellularSequence analysisAdd BLAST19
    Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
    Topological domaini165 – 172CytoplasmicSequence analysis8
    Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
    Topological domaini194 – 200ExtracellularSequence analysis7
    Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
    Topological domaini222 – 233CytoplasmicSequence analysisAdd BLAST12
    Transmembranei234 – 254HelicalSequence analysisAdd BLAST21
    Topological domaini255 – 269ExtracellularSequence analysisAdd BLAST15
    Transmembranei270 – 290HelicalSequence analysisAdd BLAST21
    Topological domaini291 – 325CytoplasmicSequence analysisAdd BLAST35
    Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
    Topological domaini347 – 367ExtracellularSequence analysisAdd BLAST21
    Transmembranei368 – 388HelicalSequence analysisAdd BLAST21
    Topological domaini389 – 396CytoplasmicSequence analysis8
    Transmembranei397 – 417HelicalSequence analysisAdd BLAST21
    Topological domaini418 – 422ExtracellularSequence analysis5
    Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
    Topological domaini444 – 461CytoplasmicSequence analysisAdd BLAST18
    Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
    Topological domaini483 – 487ExtracellularSequence analysis5
    Transmembranei488 – 508HelicalSequence analysisAdd BLAST21
    Topological domaini509 – 521CytoplasmicSequence analysisAdd BLAST13

    GO - Cellular componenti

    • integral component of fungal-type vacuolar membrane Source: GO_Central
    • plasma membrane Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002578101 – 521Riboflavin transporter MCH5Add BLAST521

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi125N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi419N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ08777.

    Interactioni

    Protein-protein interaction databases

    BioGridi34695. 7 interactors.
    IntActiQ08777. 1 interactor.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08777.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00730000112463.
    HOGENOMiHOG000162199.
    InParanoidiQ08777.
    OMAiDEDSIHY.
    OrthoDBiEOG092C20ZN.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08777-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSDSLTPKD TIVPEEQTNQ LRQPDLDEDS IHYDPEADDL ESLETTASYA
    60 70 80 90 100
    STSVSAKVYT KKEVNKGTDI ESQPHWGENT SSTHDSDKEE DSNEEIESFP
    110 120 130 140 150
    EGGFKAWVVT FGCFLGLIAC FGLLNSTGVI ESHLQDNQLS SESVSTIGWL
    160 170 180 190 200
    FSLFLFVCSA SCIISGTYFD RNGFRTIMIV GTVFHVAGLF ATANSTKYWH
    210 220 230 240 250
    FILSFAIVCG FGNGIVLSPL VSVPAHYFFK RRGTALAMAT IGGSVGGVVF
    260 270 280 290 300
    PIMLRSFFSM KSDTDPTYGF VWGIRTLGFL DLALLTLSII LVKERLPHVI
    310 320 330 340 350
    ENSKDGESRW RYILRVYILQ CFDAKAFLDM KYLFCVLGTV FSELSINSAL
    360 370 380 390 400
    TYYGSYATSH GISANDAYTL IMIINVCGIP GRWVPGYLSD KFGRFNVAIA
    410 420 430 440 450
    TLLTLFIVMF VGWLPFGTNL TNMYVISALY GFCSGSVFSL LPVCCGQISK
    460 470 480 490 500
    TEEFGKRYST MYFVVGFGTL VGIPITGAII SIKTTADYQH YIIFCGLATF
    510 520
    VSAVCYIISR AYCVGFKWVR F
    Length:521
    Mass (Da):57,582
    Last modified:September 21, 2011 - v2
    Checksum:iE80E29340A7D5B9C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti495C → S (PubMed:9153758).Curated1
    Sequence conflicti495C → S in CAA99626 (PubMed:9169874).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z75214 Genomic DNA. Translation: CAA99626.1.
    BK006948 Genomic DNA. Translation: DAA11072.2.
    PIRiS67210.
    RefSeqiNP_014951.4. NM_001183726.4.

    Genome annotation databases

    EnsemblFungiiYOR306C; YOR306C; YOR306C.
    GeneIDi854483.
    KEGGisce:YOR306C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z75214 Genomic DNA. Translation: CAA99626.1.
    BK006948 Genomic DNA. Translation: DAA11072.2.
    PIRiS67210.
    RefSeqiNP_014951.4. NM_001183726.4.

    3D structure databases

    ProteinModelPortaliQ08777.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi34695. 7 interactors.
    IntActiQ08777. 1 interactor.

    Protein family/group databases

    TCDBi2.A.1.13.4. the major facilitator superfamily (mfs).

    Proteomic databases

    PRIDEiQ08777.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYOR306C; YOR306C; YOR306C.
    GeneIDi854483.
    KEGGisce:YOR306C.

    Organism-specific databases

    EuPathDBiFungiDB:YOR306C.
    SGDiS000005833. MCH5.

    Phylogenomic databases

    GeneTreeiENSGT00730000112463.
    HOGENOMiHOG000162199.
    InParanoidiQ08777.
    OMAiDEDSIHY.
    OrthoDBiEOG092C20ZN.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33790-MONOMER.

    Miscellaneous databases

    PROiQ08777.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMCH5_YEAST
    AccessioniPrimary (citable) accession number: Q08777
    Secondary accession number(s): D6W306
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: September 21, 2011
    Last modified: November 2, 2016
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.