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Protein

Riboflavin transporter MCH5

Gene

MCH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Riboflavin transporter involved in riboflavin (vitamin B2) uptake. Does not act in the transport of monocarboxylic acids across the plasma membrane.1 Publication

Kineticsi

  1. KM=17 µM for riboflavin1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    GO - Molecular functioni

    • riboflavin transmembrane transporter activity Source: SGD

    GO - Biological processi

    • ion transmembrane transport Source: GO_Central
    • riboflavin transport Source: SGD

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33790-MONOMER

    Protein family/group databases

    TCDBi2.A.1.13.4 the major facilitator superfamily (mfs)

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin transporter MCH5
    Gene namesi
    Name:MCH5
    Ordered Locus Names:YOR306C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR306C
    SGDiS000005833 MCH5

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 103CytoplasmicSequence analysisAdd BLAST103
    Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
    Topological domaini125 – 143ExtracellularSequence analysisAdd BLAST19
    Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
    Topological domaini165 – 172CytoplasmicSequence analysis8
    Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
    Topological domaini194 – 200ExtracellularSequence analysis7
    Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
    Topological domaini222 – 233CytoplasmicSequence analysisAdd BLAST12
    Transmembranei234 – 254HelicalSequence analysisAdd BLAST21
    Topological domaini255 – 269ExtracellularSequence analysisAdd BLAST15
    Transmembranei270 – 290HelicalSequence analysisAdd BLAST21
    Topological domaini291 – 325CytoplasmicSequence analysisAdd BLAST35
    Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
    Topological domaini347 – 367ExtracellularSequence analysisAdd BLAST21
    Transmembranei368 – 388HelicalSequence analysisAdd BLAST21
    Topological domaini389 – 396CytoplasmicSequence analysis8
    Transmembranei397 – 417HelicalSequence analysisAdd BLAST21
    Topological domaini418 – 422ExtracellularSequence analysis5
    Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
    Topological domaini444 – 461CytoplasmicSequence analysisAdd BLAST18
    Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
    Topological domaini483 – 487ExtracellularSequence analysis5
    Transmembranei488 – 508HelicalSequence analysisAdd BLAST21
    Topological domaini509 – 521CytoplasmicSequence analysisAdd BLAST13

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002578101 – 521Riboflavin transporter MCH5Add BLAST521

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi419N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ08777
    PRIDEiQ08777

    Interactioni

    Protein-protein interaction databases

    BioGridi34695, 23 interactors
    IntActiQ08777, 1 interactor
    STRINGi4932.YOR306C

    Structurei

    3D structure databases

    ProteinModelPortaliQ08777
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00730000112463
    HOGENOMiHOG000162199
    InParanoidiQ08777
    OMAiHCCVCGA
    OrthoDBiEOG092C20ZN

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR011701 MFS
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    PfamiView protein in Pfam
    PF07690 MFS_1, 1 hit
    SUPFAMiSSF103473 SSF103473, 1 hit
    PROSITEiView protein in PROSITE
    PS50850 MFS, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q08777-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSDSLTPKD TIVPEEQTNQ LRQPDLDEDS IHYDPEADDL ESLETTASYA
    60 70 80 90 100
    STSVSAKVYT KKEVNKGTDI ESQPHWGENT SSTHDSDKEE DSNEEIESFP
    110 120 130 140 150
    EGGFKAWVVT FGCFLGLIAC FGLLNSTGVI ESHLQDNQLS SESVSTIGWL
    160 170 180 190 200
    FSLFLFVCSA SCIISGTYFD RNGFRTIMIV GTVFHVAGLF ATANSTKYWH
    210 220 230 240 250
    FILSFAIVCG FGNGIVLSPL VSVPAHYFFK RRGTALAMAT IGGSVGGVVF
    260 270 280 290 300
    PIMLRSFFSM KSDTDPTYGF VWGIRTLGFL DLALLTLSII LVKERLPHVI
    310 320 330 340 350
    ENSKDGESRW RYILRVYILQ CFDAKAFLDM KYLFCVLGTV FSELSINSAL
    360 370 380 390 400
    TYYGSYATSH GISANDAYTL IMIINVCGIP GRWVPGYLSD KFGRFNVAIA
    410 420 430 440 450
    TLLTLFIVMF VGWLPFGTNL TNMYVISALY GFCSGSVFSL LPVCCGQISK
    460 470 480 490 500
    TEEFGKRYST MYFVVGFGTL VGIPITGAII SIKTTADYQH YIIFCGLATF
    510 520
    VSAVCYIISR AYCVGFKWVR F
    Length:521
    Mass (Da):57,582
    Last modified:September 21, 2011 - v2
    Checksum:iE80E29340A7D5B9C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti495C → S (PubMed:9153758).Curated1
    Sequence conflicti495C → S in CAA99626 (PubMed:9169874).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z75214 Genomic DNA Translation: CAA99626.1
    BK006948 Genomic DNA Translation: DAA11072.2
    PIRiS67210
    RefSeqiNP_014951.4, NM_001183726.4

    Genome annotation databases

    EnsemblFungiiYOR306C; YOR306C; YOR306C
    GeneIDi854483
    KEGGisce:YOR306C

    Similar proteinsi

    Entry informationi

    Entry nameiMCH5_YEAST
    AccessioniPrimary (citable) accession number: Q08777
    Secondary accession number(s): D6W306
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: September 21, 2011
    Last modified: May 23, 2018
    This is version 130 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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