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Protein

ISWI chromatin-remodeling complex ATPase ISW2

Gene

ISW2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. The ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex.7 Publications

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 216ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • centromeric DNA binding Source: SGD
  • chromatin binding Source: SGD
  • DNA translocase activity Source: SGD
  • helicase activity Source: UniProtKB-KW
  • nucleosome binding Source: InterPro
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: InterPro
  • chromatin remodeling Source: SGD
  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at telomere Source: SGD
  • covalent chromatin modification Source: UniProtKB-KW
  • negative regulation of antisense RNA transcription Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
  • nucleosome positioning Source: SGD
  • termination of RNA polymerase II transcription Source: SGD

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Helicase, Hydrolase, Repressor
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33788-MONOMER.
ReactomeiR-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
ISWI chromatin-remodeling complex ATPase ISW2 (EC:3.6.4.-)
Alternative name(s):
Imitation switch protein 2
Gene namesi
Name:ISW2
Ordered Locus Names:YOR304W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR304W.
SGDiS000005831. ISW2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi215K → A: Abolishes ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002404531 – 1120ISWI chromatin-remodeling complex ATPase ISW2Add BLAST1120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineCombined sources1
Modified residuei19PhosphoserineCombined sources1
Modified residuei831PhosphoserineCombined sources1
Modified residuei1079PhosphothreonineCombined sources1
Modified residuei1082PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08773.
PRIDEiQ08773.

PTM databases

iPTMnetiQ08773.

Interactioni

Subunit structurei

Component of the ISW2 complex, which at least consists of ISW2, ITC1, DLS1 and DPB4. May form a stable subcomplex with ITC1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34692. 335 interactors.
DIPiDIP-6603N.
IntActiQ08773. 53 interactors.
MINTiMINT-469536.
STRINGi4932.YOR304W.

Structurei

3D structure databases

ProteinModelPortaliQ08773.
SMRiQ08773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini196 – 361Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini494 – 645Helicase C-terminalPROSITE-ProRule annotationAdd BLAST152
Domaini886 – 938SANTPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi312 – 315DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00860000133761.
HOGENOMiHOG000192862.
InParanoidiQ08773.
KOiK11654.
OMAiMAFTEWI.
OrthoDBiEOG092C1YH4.

Family and domain databases

InterProiView protein in InterPro
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeobox-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
PANTHERiPTHR10799:SF812. PTHR10799:SF812. 1 hit.
PfamiView protein in Pfam
PF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
SUPFAMiSSF101224. SSF101224. 2 hits.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQQEEQRS DTKNSKSESP SEVLVDTLDS KSNGSSDDDN IGQSEELSDK
60 70 80 90 100
EIYTVEDRPP EYWAQRKKKF VLDVDPKYAK QKDKSDTYKR FKYLLGVTDL
110 120 130 140 150
FRHFIGIKAK HDKNIQKLLK QLDSDANKLS KSHSTVSSSS RHHRKTEKEE
160 170 180 190 200
DAELMADEEE EIVDTYQEDI FVSESPSFVK SGKLRDYQVQ GLNWLISLHE
210 220 230 240 250
NKLSGILADE MGLGKTLQTI SFLGYLRYVK QIEGPFLIIV PKSTLDNWRR
260 270 280 290 300
EFLKWTPNVN VLVLHGDKDT RADIVRNIIL EARFDVLITS YEMVIREKNA
310 320 330 340 350
LKRLAWQYIV IDEAHRIKNE QSALSQIIRL FYSKNRLLIT GTPLQNNLHE
360 370 380 390 400
LWALLNFLLP DIFGDSELFD EWFEQNNSEQ DQEIVIQQLH SVLNPFLLRR
410 420 430 440 450
VKADVEKSLL PKIETNVYVG MTDMQIQWYK SLLEKDIDAV NGAVGKREGK
460 470 480 490 500
TRLLNIVMQL RKCCNHPYLF EGAEPGPPYT TDEHLIFNSG KMIILDKLLK
510 520 530 540 550
RLKEKGSRVL IFSQMSRLLD ILEDYCYFRD FEYCRIDGST SHEERIEAID
560 570 580 590 600
EYNKPNSEKF VFLLTTRAGG LGINLVTADT VILFDSDWNP QADLQAMDRA
610 620 630 640 650
HRIGQKKQVH VYRFVTENAI EEKVIERAAQ KLRLDQLVIQ QGTGKKTASL
660 670 680 690 700
GNSKDDLLDM IQFGAKNMFE KKASKVTVDA DIDDILKKGE QKTQELNAKY
710 720 730 740 750
QSLGLDDLQK FNGIENQSAY EWNGKSFQKK SNDKVVEWIN PSRRERRREQ
760 770 780 790 800
TTYSVDDYYK EIIGGGSKSA SKQTPQPKAP RAPKVIHGQD FQFFPKELDA
810 820 830 840 850
LQEKEQLYFK KKVNYKVTSY DITGDIRNEG SDAEEEEGEY KNAANTEGHK
860 870 880 890 900
GHEELKRRIE EEQEKINSAP DFTQEDELRK QELISKAFTN WNKRDFMAFI
910 920 930 940 950
NACAKYGRDD MENIKKSIDS KTPEEVEVYA KIFWERLKEI NGWEKYLHNV
960 970 980 990 1000
ELGEKKNEKL KFQETLLRQK IEQCKHPLHE LIIQYPPNNA RRTYNTLEDK
1010 1020 1030 1040 1050
FLLLAVNKYG LRADKLYEKL KQEIMMSDLF TFDWFIKTRT VHELSKRVHT
1060 1070 1080 1090 1100
LLTLIVREYE QPDANKKKRS RTSATREDTP LSQNESTRAS TVPNLPTTMV
1110 1120
TNQKDTNDHV DKRTKIDQEA
Length:1,120
Mass (Da):130,327
Last modified:November 1, 1996 - v1
Checksum:iF71007D7B25C9348
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75212 Genomic DNA. Translation: CAA99622.1.
BK006948 Genomic DNA. Translation: DAA11069.1.
PIRiS67208.
RefSeqiNP_014948.1. NM_001183724.1.

Genome annotation databases

EnsemblFungiiYOR304W; YOR304W; YOR304W.
GeneIDi854480.
KEGGisce:YOR304W.

Similar proteinsi

Entry informationi

Entry nameiISW2_YEAST
AccessioniPrimary (citable) accession number: Q08773
Secondary accession number(s): D6W303
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names