Q08749 (TIM18_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM18

Gene names

Name:	TIM18
Ordered Locus Names:	YOR297C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. Its role in the complex is unclear but it may be involved in the assembly and stabilization of the TIM22 complex. Ref.1 Ref.5 Ref.6
Subunit structure	Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Ref.1 Ref.5 Ref.7.
Disruption phenotype	Although cells lacking TIM18 live well and do not display any strong phenotype, they do not survive in the absence of mitochondrial DNA. Ref.6
Miscellaneous	Present with 4440 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the CybS family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from mutant phenotype. Source: SGD cellular response to oxidative stress Inferred from mutant phenotype. Source: SGD protein import into mitochondrial inner membrane Inferred from genetic interaction Ref.5. Source: SGD response to acid Inferred from mutant phenotype. Source: SGD response to arsenic-containing substance Inferred from mutant phenotype. Source: SGD response to osmotic stress Inferred from mutant phenotype. Source: SGD tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane protein insertion complex Inferred from direct assay Ref.5Ref.1. Source: SGD
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Mitochondrion Potential
	Chain	43 – 192	150	Mitochondrial import inner membrane translocase subunit TIM18		PRO_0000228080
Regions
	Topological domain	43 – 88	46	Mitochondrial matrix Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Topological domain	110 – 113	4	Mitochondrial intermembrane Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Topological domain	135 – 144	10	Mitochondrial matrix Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Topological domain	166 – 192	27	Mitochondrial intermembrane Potential
Experimental info
	Sequence conflict	137	1	E → G in AAF13717. Ref.1
	Sequence conflict	137	1	E → G Ref.2
	Sequence conflict	137	1	E → G in CAA99525. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q08749 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: FE5DBEF3E3446CF7	FASTA	192	21,972
	10 20 30 40 50 60 MLLFPGLKPV LNASTVIVNP VRAVFPGLVL STKRSFYSIN RLNAENKIND IANTSKEASS 70 80 90 100 110 120 SVQMFKPPEF SQFKDSYQKD YERIAKYTLI PLTMVPFYAS FTGGVINPLL DASLSSIFLI 130 140 150 160 170 180 YLQYGFTSCI IDYIPKEKYP RWHKLALYCL YGGSMLSLYG IYELETKNNG FVDLVKKLWN 190 ENDDHLYIFG RN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane." Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E. Mol. Cell. Biol. 20:1187-1193(2000) [PubMed: 10648604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM12; TIM22 AND TIM54. Strain: ATCC 24657 / D273-10B.
[2]	"Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue." Poirey R., Cziepluch C., Tobiasch E., Pujol A., Kordes E., Jauniaux J.-C. Yeast 13:479-482(1997) [PubMed: 9153758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 137. Strain: ATCC 204508 / S288c.
[5]	"Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane." Kerscher O., Sepuri N.B., Jensen R.E. Mol. Biol. Cell 11:103-116(2000) [PubMed: 10637294] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM22 AND TIM54.
[6]	"Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA." Dunn C.D., Jensen R.E. Genetics 165:35-45(2003) [PubMed: 14504216] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Protein insertion into the mitochondrial inner membrane by a twin-pore translocase." Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N. Science 299:1747-1751(2003) [PubMed: 12637749] [Abstract] Cited for: IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM12; TIM22 AND TIM54.
[10]	Erratum Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N. Science 300:251-251(2003)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF200324 Genomic DNA. Translation: AAF13717.1. Z75205 Genomic DNA. Translation: CAA99525.1. BK006948 Genomic DNA. Translation: DAA11062.2.
PIR	S67201.
RefSeq	NP_014940.2. NM_001183716.2.
3D structure databases
ProteinModelPortal	Q08749.
SMR	Q08749. Positions 71-181.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5205N.
IntAct	Q08749. 4 interactions.
MINT	MINT-532619.
STRING	Q08749.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	854472.
KEGG	sce:YOR297C.
NMPDR	fig|4932.3.peg.6053.
Organism-specific databases
CYGD	YOR297c.
SGD	S000005823. TIM18.
Phylogenomic databases
eggNOG	fuNOG11585.
GeneTree	EFGT00050000005635.
OMA	NDIANTS.
OrthoDB	EOG4W9NDM.
Gene expression databases
ArrayExpress	Q08749.
Genevestigator	Q08749.
GermOnline	YOR297C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007992. Cyt_b_succ_DH_CybS. [Graphical view]
PANTHER	PTHR13337. CybS. 1 hit.
Pfam	PF05328. CybS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976771.

Entry information

Entry name

TIM18_YEAST

Accession

Primary (citable) accession number: Q08749
Secondary accession number(s): D6W2Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program