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Protein

Mitochondrial import inner membrane translocase subunit TIM18

Gene

TIM18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. Its role in the complex is unclear but it may be involved in the assembly and stabilization of the TIM22 complex.3 Publications

Miscellaneous

Present with 4440 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • apoptotic process Source: SGD
  • cellular response to oxidative stress Source: SGD
  • protein import into mitochondrial inner membrane Source: SGD
  • response to arsenic-containing substance Source: SGD
  • response to osmotic stress Source: SGD

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33782-MONOMER

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM18
Gene namesi
Name:TIM18
Ordered Locus Names:YOR297C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR297C
SGDiS000005823 TIM18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini43 – 88Mitochondrial matrixSequence analysisAdd BLAST46
Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
Topological domaini110 – 113Mitochondrial intermembraneSequence analysis4
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Topological domaini135 – 144Mitochondrial matrixSequence analysis10
Transmembranei145 – 165HelicalSequence analysisAdd BLAST21
Topological domaini166 – 192Mitochondrial intermembraneSequence analysisAdd BLAST27

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Although cells lacking TIM18 live well and do not display any strong phenotype, they do not survive in the absence of mitochondrial DNA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000022808043 – 192Mitochondrial import inner membrane translocase subunit TIM18Add BLAST150

Proteomic databases

MaxQBiQ08749
PaxDbiQ08749
PRIDEiQ08749

PTM databases

iPTMnetiQ08749

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM10P871082EBI-30499,EBI-9115

Protein-protein interaction databases

BioGridi34685, 300 interactors
DIPiDIP-5205N
IntActiQ08749, 5 interactors
MINTiQ08749
STRINGi4932.YOR297C

Structurei

3D structure databases

ProteinModelPortaliQ08749
SMRiQ08749
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CybS family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009201
HOGENOMiHOG000204209
InParanoidiQ08749
KOiK17791
OMAiRTTETHD
OrthoDBiEOG092C5DUX

Family and domain databases

CDDicd03496 SQR_TypeC_CybS, 1 hit
Gene3Di1.20.1300.10, 1 hit
InterProiView protein in InterPro
IPR007992 CybS
IPR034804 SQR/QFR_C/D
PANTHERiPTHR13337 PTHR13337, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFPGLKPV LNASTVIVNP VRAVFPGLVL STKRSFYSIN RLNAENKIND
60 70 80 90 100
IANTSKEASS SVQMFKPPEF SQFKDSYQKD YERIAKYTLI PLTMVPFYAS
110 120 130 140 150
FTGGVINPLL DASLSSIFLI YLQYGFTSCI IDYIPKEKYP RWHKLALYCL
160 170 180 190
YGGSMLSLYG IYELETKNNG FVDLVKKLWN ENDDHLYIFG RN
Length:192
Mass (Da):21,972
Last modified:September 21, 2011 - v2
Checksum:iFE5DBEF3E3446CF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137E → G in AAF13717 (PubMed:10648604).Curated1
Sequence conflicti137E → G (PubMed:9153758).Curated1
Sequence conflicti137E → G in CAA99525 (PubMed:9169874).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200324 Genomic DNA Translation: AAF13717.1
Z75205 Genomic DNA Translation: CAA99525.1
BK006948 Genomic DNA Translation: DAA11062.2
PIRiS67201
RefSeqiNP_014940.4, NM_001183716.4

Genome annotation databases

EnsemblFungiiYOR297C; YOR297C; YOR297C
GeneIDi854472
KEGGisce:YOR297C

Similar proteinsi

Entry informationi

Entry nameiTIM18_YEAST
AccessioniPrimary (citable) accession number: Q08749
Secondary accession number(s): D6W2Z6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: September 21, 2011
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health