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Protein

Mitochondrial import inner membrane translocase subunit TIM18

Gene

TIM18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. Its role in the complex is unclear but it may be involved in the assembly and stabilization of the TIM22 complex.3 Publications

GO - Biological processi

  • apoptotic process Source: SGD
  • cellular response to oxidative stress Source: SGD
  • protein import into mitochondrial inner membrane Source: SGD
  • response to arsenic-containing substance Source: SGD
  • response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33782-MONOMER.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM18
Gene namesi
Name:TIM18
Ordered Locus Names:YOR297C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR297C.
SGDiS000005823. TIM18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini43 – 8846Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Topological domaini110 – 1134Mitochondrial intermembraneSequence analysis
Transmembranei114 – 13421HelicalSequence analysisAdd
BLAST
Topological domaini135 – 14410Mitochondrial matrixSequence analysis
Transmembranei145 – 16521HelicalSequence analysisAdd
BLAST
Topological domaini166 – 19227Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Although cells lacking TIM18 live well and do not display any strong phenotype, they do not survive in the absence of mitochondrial DNA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242MitochondrionSequence analysisAdd
BLAST
Chaini43 – 192150Mitochondrial import inner membrane translocase subunit TIM18PRO_0000228080Add
BLAST

Proteomic databases

MaxQBiQ08749.

PTM databases

iPTMnetiQ08749.

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM10P871082EBI-30499,EBI-9115

Protein-protein interaction databases

BioGridi34685. 190 interactions.
DIPiDIP-5205N.
IntActiQ08749. 4 interactions.
MINTiMINT-532619.

Structurei

3D structure databases

ProteinModelPortaliQ08749.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CybS family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009201.
HOGENOMiHOG000204209.
InParanoidiQ08749.
KOiK17791.
OMAiRTTETHD.
OrthoDBiEOG76MKMX.

Family and domain databases

InterProiIPR007992. CybS.
[Graphical view]
PANTHERiPTHR13337. PTHR13337. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFPGLKPV LNASTVIVNP VRAVFPGLVL STKRSFYSIN RLNAENKIND
60 70 80 90 100
IANTSKEASS SVQMFKPPEF SQFKDSYQKD YERIAKYTLI PLTMVPFYAS
110 120 130 140 150
FTGGVINPLL DASLSSIFLI YLQYGFTSCI IDYIPKEKYP RWHKLALYCL
160 170 180 190
YGGSMLSLYG IYELETKNNG FVDLVKKLWN ENDDHLYIFG RN
Length:192
Mass (Da):21,972
Last modified:September 21, 2011 - v2
Checksum:iFE5DBEF3E3446CF7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 1371E → G in AAF13717 (PubMed:10648604).Curated
Sequence conflicti137 – 1371E → G (PubMed:9153758).Curated
Sequence conflicti137 – 1371E → G in CAA99525 (PubMed:9169874).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200324 Genomic DNA. Translation: AAF13717.1.
Z75205 Genomic DNA. Translation: CAA99525.1.
BK006948 Genomic DNA. Translation: DAA11062.2.
PIRiS67201.
RefSeqiNP_014940.4. NM_001183716.4.

Genome annotation databases

EnsemblFungiiYOR297C; YOR297C; YOR297C.
GeneIDi854472.
KEGGisce:YOR297C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200324 Genomic DNA. Translation: AAF13717.1.
Z75205 Genomic DNA. Translation: CAA99525.1.
BK006948 Genomic DNA. Translation: DAA11062.2.
PIRiS67201.
RefSeqiNP_014940.4. NM_001183716.4.

3D structure databases

ProteinModelPortaliQ08749.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34685. 190 interactions.
DIPiDIP-5205N.
IntActiQ08749. 4 interactions.
MINTiMINT-532619.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

PTM databases

iPTMnetiQ08749.

Proteomic databases

MaxQBiQ08749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR297C; YOR297C; YOR297C.
GeneIDi854472.
KEGGisce:YOR297C.

Organism-specific databases

EuPathDBiFungiDB:YOR297C.
SGDiS000005823. TIM18.

Phylogenomic databases

GeneTreeiENSGT00390000009201.
HOGENOMiHOG000204209.
InParanoidiQ08749.
KOiK17791.
OMAiRTTETHD.
OrthoDBiEOG76MKMX.

Enzyme and pathway databases

BioCyciYEAST:G3O-33782-MONOMER.

Miscellaneous databases

NextBioi976771.
PROiQ08749.

Family and domain databases

InterProiIPR007992. CybS.
[Graphical view]
PANTHERiPTHR13337. PTHR13337. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane."
    Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E.
    Mol. Cell. Biol. 20:1187-1193(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM12; TIM22 AND TIM54.
    Strain: ATCC 24657 / D273-10B.
  2. "Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue."
    Poirey R., Cziepluch C., Tobiasch E., Pujol A., Kordes E., Jauniaux J.-C.
    Yeast 13:479-482(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 137.
    Strain: ATCC 204508 / S288c.
  5. "Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane."
    Kerscher O., Sepuri N.B., Jensen R.E.
    Mol. Biol. Cell 11:103-116(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM22 AND TIM54.
  6. "Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA."
    Dunn C.D., Jensen R.E.
    Genetics 165:35-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Protein insertion into the mitochondrial inner membrane by a twin-pore translocase."
    Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N.
    Science 299:1747-1751(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM12; TIM22 AND TIM54.

Entry informationi

Entry nameiTIM18_YEAST
AccessioniPrimary (citable) accession number: Q08749
Secondary accession number(s): D6W2Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: September 21, 2011
Last modified: May 11, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.