Q08747 (UAF30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Upstream activation factor subunit UAF30

Alternative name(s):
Upstream activation factor 30 KDa subunit
Short name=p30

Gene names

Name:	UAF30
Ordered Locus Names:	YOR295W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nonessential component of the UAF (upstream activation factor) complex which interacts with the upstream element of the RNA polymerase I promoter and forms a stable preinitiation complex. Together with SPT15/TBP UAF seems to stimulate basal transcription to a fully activated level. UAF30 seems to play a role in silencing transcription by RNA polymerase II.
Subunit structure	Component of the UAF (upstream activation factor) complex which consists of UAF30, RRN5, RRN9, RRN10, and histones H3 and H4.
Subcellular location	Nucleus › nucleolus Ref.4.
Miscellaneous	Present with 639 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 SWIB domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin organization Inferred from mutant phenotype. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription of nuclear large rRNA transcript from RNA polymerase I promoter Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	RNA polymerase I upstream activating factor complex Inferred from direct assay Ref.3. Source: SGD
Molecular function	RNA polymerase I upstream control element sequence-specific DNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Upstream activation factor subunit UAF30		PRO_0000245507
Regions
	Domain	120 – 194	75	SWIB
Amino acid modifications
	Modified residue	218	1	Phosphoserine Ref.6 Ref.8
	Modified residue	220	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	223	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q08747 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C480D943D728021A	FASTA	228	25,967
	10 20 30 40 50 60 MAELNDYSTM IDILLSDMDL ETVTTKKVRM ALKEVYAIDV ESQGKAINKL IRKHLDLVKE 70 80 90 100 110 120 RPRFERSLED LLKENATLAI ELTKEITVSK RSSGEEKNDS ETKGTHVEKK KGTVSKSPIS 130 140 150 160 170 180 TRKVTLSKSL ASLLGEHELT RTEVVRRLWA YIKAHNLQNP NNKKEILCDE KLELILGKST 190 200 210 220 NMFEMHKILA SHMTEPKKIS DCPPLIQEVR RKEKPIVSDS EQSDTKGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Transcription of chromosomal rRNA genes by both RNA polymerase I and II in yeast uaf30 mutants lacking the 30 kDa subunit of transcription factor UAF." Siddiqi I.N., Dodd J.A., Vu L., Eliason K., Oakes M.L., Keener J., Moore R., Young M.K., Nomura M. EMBO J. 20:4512-4521(2001) [PubMed: 11500378] [Abstract] Cited for: IDENTIFICATION IN THE UAF COMPLEX.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218; SER-220 AND SER-223, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218 AND SER-220, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75203 Genomic DNA. Translation: CAA99523.1. BK006948 Genomic DNA. Translation: DAA11060.1.
PIR	S67199.
RefSeq	NP_014938.1. NM_001183714.1.
3D structure databases
ProteinModelPortal	Q08747.
SMR	Q08747. Positions 1-55, 115-201.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08747. 6 interactions.
STRING	Q08747.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR295W; YOR295W; YOR295W.
GeneID	854470.
KEGG	sce:YOR295W.
NMPDR	fig|4932.3.peg.6051.
Organism-specific databases
CYGD	YOR295w.
SGD	S000005821. UAF30.
Phylogenomic databases
eggNOG	fuNOG12560.
GeneTree	EFGT00050000004361.
HOGENOM	HBG203497.
OrthoDB	EOG486CP2.
Gene expression databases
ArrayExpress	Q08747.
Genevestigator	Q08747.
GermOnline	YOR295W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014876. DEK_C. IPR019835. SWIB_domain. IPR003121. SWIB_MDM2_domain. [Graphical view]
KO	K15223.
Pfam	PF08766. DEK_C. 1 hit. PF02201. SWIB. 1 hit. [Graphical view]
SMART	SM00151. SWIB. 1 hit. [Graphical view]
SUPFAM	SSF47592. MDM2. 1 hit.
ProtoNet	Search...
Other
NextBio	976765.

Entry information

Entry name

UAF30_YEAST

Accession

Primary (citable) accession number: Q08747
Secondary accession number(s): D6W2Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program