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Protein

40S ribosomal protein S10-A

Gene

RPS10A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as a positive regulator of the GCN2 kinase activity by stimulating GCN1-mediated GCN2 activation (PubMed:25437641).1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cellular response to amino acid starvation Source: UniProtKB
  • cytoplasmic translation Source: SGD
  • positive regulation of protein kinase activity Source: UniProtKB
  • rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33778-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S10-A
Gene namesi
Name:RPS10A
Ordered Locus Names:YOR293W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR293W.
SGDiS000005819. RPS10A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10510540S ribosomal protein S10-APRO_0000116376Add
BLAST

Post-translational modificationi

The N-terminus is not modified.

Proteomic databases

MaxQBiQ08745.
TopDownProteomicsiQ08745.

PTM databases

iPTMnetiQ08745.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit (PubMed:9559554). The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S) (PubMed:9559554). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S) (PubMed:9559554). Interacts with GCN1 (via middle region); this interaction is direct and promotes GCN2 kinase activity (PubMed:25437641).2 Publications

Protein-protein interaction databases

BioGridi34681. 78 interactions.
IntActiQ08745. 10 interactions.
MINTiMINT-2786873.

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1814Combined sources
Beta strandi19 – 257Combined sources
Beta strandi33 – 375Combined sources
Helixi39 – 5113Combined sources
Beta strandi53 – 564Combined sources
Turni60 – 623Combined sources
Beta strandi63 – 686Combined sources
Helixi70 – 7910Combined sources
Helixi88 – 903Combined sources
Turni93 – 953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10101-105[»]
3J6Yelectron microscopy6.10101-105[»]
3J77electron microscopy6.20101-105[»]
3J78electron microscopy6.30101-105[»]
3V88X-ray3.00K1-105[»]
4U3NX-ray3.20C0/c01-105[»]
4U3UX-ray2.90C0/c01-105[»]
4U4NX-ray3.10C0/c01-105[»]
4U4OX-ray3.60C0/c01-105[»]
4U4QX-ray3.00C0/c01-105[»]
4U4RX-ray2.80C0/c01-105[»]
4U4UX-ray3.00C0/c01-105[»]
4U4YX-ray3.20C0/c01-105[»]
4U4ZX-ray3.10C0/c01-105[»]
4U50X-ray3.20C0/c01-105[»]
4U51X-ray3.20C0/c01-105[»]
4U52X-ray3.00C0/c01-105[»]
4U53X-ray3.30C0/c01-105[»]
4U55X-ray3.20C0/c01-105[»]
4U56X-ray3.45C0/c01-105[»]
4U6FX-ray3.10C0/c01-105[»]
4V88X-ray3.00AK/CK1-105[»]
4V8Yelectron microscopy4.30AK1-105[»]
4V8Zelectron microscopy6.60AK1-105[»]
4V92electron microscopy3.70K1-93[»]
ProteinModelPortaliQ08745.
SMRiQ08745. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S10e family.Curated

Phylogenomic databases

GeneTreeiENSGT00440000034918.
HOGENOMiHOG000216624.
InParanoidiQ08745.
KOiK02947.
OMAiMEYLREW.
OrthoDBiEOG74TXC4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005326. S10_plectin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03501. S10_plectin. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT
60 70 80 90 100
SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPGT YIQERNPTQR

PQRRY
Length:105
Mass (Da):12,739
Last modified:November 1, 1996 - v1
Checksum:iE104B39336EE64BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75201 Genomic DNA. Translation: CAA99521.1.
BK006948 Genomic DNA. Translation: DAA11057.1.
PIRiS67197.
RefSeqiNP_014936.1. NM_001183712.1.

Genome annotation databases

EnsemblFungiiYOR293W; YOR293W; YOR293W.
GeneIDi854468.
KEGGisce:YOR293W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75201 Genomic DNA. Translation: CAA99521.1.
BK006948 Genomic DNA. Translation: DAA11057.1.
PIRiS67197.
RefSeqiNP_014936.1. NM_001183712.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10101-105[»]
3J6Yelectron microscopy6.10101-105[»]
3J77electron microscopy6.20101-105[»]
3J78electron microscopy6.30101-105[»]
3V88X-ray3.00K1-105[»]
4U3NX-ray3.20C0/c01-105[»]
4U3UX-ray2.90C0/c01-105[»]
4U4NX-ray3.10C0/c01-105[»]
4U4OX-ray3.60C0/c01-105[»]
4U4QX-ray3.00C0/c01-105[»]
4U4RX-ray2.80C0/c01-105[»]
4U4UX-ray3.00C0/c01-105[»]
4U4YX-ray3.20C0/c01-105[»]
4U4ZX-ray3.10C0/c01-105[»]
4U50X-ray3.20C0/c01-105[»]
4U51X-ray3.20C0/c01-105[»]
4U52X-ray3.00C0/c01-105[»]
4U53X-ray3.30C0/c01-105[»]
4U55X-ray3.20C0/c01-105[»]
4U56X-ray3.45C0/c01-105[»]
4U6FX-ray3.10C0/c01-105[»]
4V88X-ray3.00AK/CK1-105[»]
4V8Yelectron microscopy4.30AK1-105[»]
4V8Zelectron microscopy6.60AK1-105[»]
4V92electron microscopy3.70K1-93[»]
ProteinModelPortaliQ08745.
SMRiQ08745. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34681. 78 interactions.
IntActiQ08745. 10 interactions.
MINTiMINT-2786873.

PTM databases

iPTMnetiQ08745.

Proteomic databases

MaxQBiQ08745.
TopDownProteomicsiQ08745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR293W; YOR293W; YOR293W.
GeneIDi854468.
KEGGisce:YOR293W.

Organism-specific databases

EuPathDBiFungiDB:YOR293W.
SGDiS000005819. RPS10A.

Phylogenomic databases

GeneTreeiENSGT00440000034918.
HOGENOMiHOG000216624.
InParanoidiQ08745.
KOiK02947.
OMAiMEYLREW.
OrthoDBiEOG74TXC4.

Enzyme and pathway databases

BioCyciYEAST:G3O-33778-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi976759.
PROiQ08745.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005326. S10_plectin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03501. S10_plectin. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue."
    Poirey R., Cziepluch C., Tobiasch E., Pujol A., Kordes E., Jauniaux J.-C.
    Yeast 13:479-482(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ANALYSIS OF N-TERMINUS.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2."
    Lee S.J., Swanson M.J., Sattlegger E.
    Biochem. J. 466:547-559(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GCN1.

Entry informationi

Entry nameiRS10A_YEAST
AccessioniPrimary (citable) accession number: Q08745
Secondary accession number(s): D6W2Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 54100 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S10 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.