Reviewed, UniProtKB/Swiss-Prot Q08733 (PIP13_ARATH)

Names and origin

Protein names

Recommended name:
    Aquaporin PIP1-3
      Short name=AtPIP1;3
Alternative name(s):
    Plasma membrane intrinsic protein 1c
      Short name=PIP1c
    Transmembrane protein B
      Short name=TMP-B

Gene names

Name:	PIP1-3
Synonyms:	PIP1C, TMPB
Ordered Locus Names:	At1g01620
ORF Names:	F22L4.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Water channel required to facilitate the transport of water across cell membrane. Its function is impaired by Hg2+. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.2.
Tissue specificity	Expressed in roots, above ground, ripening fruit, flower buds, green siliques and senescing leaves. Ref.2 Ref.5
Domain	The Asn-Pro-Ala (NPA) motifs may contribute to the formation of two hemipores that could generate a narrow channel By similarity.
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to water deprivation Inferred from expression pattern. Source: TAIR transmembrane transport Inferred from electronic annotation. Source: InterPro water transport Ref.2 Inferred from direct assay. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.2 Inferred from direct assay. Source: TAIR
Molecular function	water channel activity Ref.2 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q08733-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Aquaporin PIP1-3		PRO_0000064048
Regions
	Topological domain	1 – 54	54	Cytoplasmic Potential
	Transmembrane	55 – 75	21	1 Potential
	Topological domain	76 – 81	6	Extracellular Potential
	Transmembrane	82 – 102	21	2 Potential
	Topological domain	103 – 132	30	Cytoplasmic Potential
	Transmembrane	133 – 153	21	3 Potential
	Topological domain	154 – 174	21	Extracellular Potential
	Transmembrane	175 – 195	21	4 Potential
	Topological domain	196 – 208	13	Cytoplasmic Potential
	Transmembrane	209 – 229	21	5 Potential
	Topological domain	230 – 256	27	Extracellular Potential
	Transmembrane	257 – 277	21	6 Potential
	Topological domain	278 – 286	9	Cytoplasmic Potential
	Motif	114 – 116	3	NPA 1
	Motif	235 – 237	3	NPA 2
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.6
Experimental info
	Sequence conflict	141	1	L → F in CAA53476. Ref.2
	Sequence conflict	189	1	V → I in CAA53476. Ref.2
	Sequence conflict	226	1	T → S in CAA53476. Ref.2
	Sequence conflict	227	1	I → F in AAN72112. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4E2F8444232030B2	FASTA	286	30,633
	10 20 30 40 50 60 MEGKEEDVRV GANKFPERQP IGTSAQTDKD YKEPPPAPFF EPGELSSWSF YRAGIAEFIA 70 80 90 100 110 120 TFLFLYITVL TVMGVKRAPN MCASVGIQGI AWAFGGMIFA LVYCTAGISG GHINPAVTFG 130 140 150 160 170 180 LFLARKLSLT RAVFYIVMQC LGAICGAGVV KGFQPNPYQT LGGGANTVAH GYTKGSGLGA 190 200 210 220 230 240 EIIGTFVLVY TVFSATDAKR SARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL 250 260 270 280 GAAIIYNKDH AWDDHWIFWV GPFIGAALAA LYHQLVIRAI PFKSRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of an Arabidopsis thaliana turgor-responsive TMP-B cDNA clone encoding transmembrane protein with a major intrinsic protein motif." Shagan T., Meraro D., Bar-Zvi D. Plant Physiol. 102:689-690(1993) [PubMed: 8108519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Etiolated seedling.
[2]	"Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system." Kammerloher W., Fischer U., Piechottka G.P., Schaeffner A.R. Plant J. 6:187-199(1994) [PubMed: 7920711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Root.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"From genome to function: the Arabidopsis aquaporins." Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J. Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed: 11806824] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[6]	"Methylation of aquaporins in plant plasma membrane." Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C. Biochem. J. 400:189-197(2006) [PubMed: 16839310] [Abstract] Cited for: ACETYLATION AT MET-1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69294 mRNA. Translation: CAA49155.1. X75882 mRNA. Translation: CAA53476.1. AC061957 Genomic DNA. Translation: AAF81320.1. AF348574 mRNA. Translation: AAK15545.1. AY062610 mRNA. Translation: AAL32688.1. BT002101 mRNA. Translation: AAN72112.1.
IPI	IPI00533886.
PIR	A86147.
RefSeq	NP_171668.1.
UniGene	At.24062 Rra.20442 Rsa.12514
3D structure databases
SMR	Q08733. Positions 37-284.
ModBase	Search...
Proteomic databases
PRIDE	Q08733.
Genome annotation databases
GeneID	839235.
GenomeReviews	Gene locus AT1G01620 in contig CT485782_GR.
NMPDR	fig|3702.1.peg.272.
Organism-specific databases
GeneFarm	4864. 182.
TAIR	At1g01620.
Phylogenomic databases
HOGENOM	HBG705794.
InParanoid	Q08733.
OMA	RTTIDSE.
Gene expression databases
ArrayExpress	Q08733.
Genevestigator	Q08733.
GermOnline	AT1G01620. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIP13_ARATH

Accession

Primary (citable) accession number: Q08733
Secondary accession number(s): Q8W4E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)