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Protein

GPN-loop GTPase 2

Gene

GPN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import (PubMed:23267056). Required for establishment of sister chromatid cohesion (PubMed:18439903).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei71 – 711Stabilizes the phosphate intermediate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176GTPBy similarity
Nucleotide bindingi175 – 1784GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • establishment of mitotic sister chromatid cohesion Source: SGD
  • RNA polymerase II complex import to nucleus Source: SGD
  • RNA polymerase III complex localization to nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33753-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GPN-loop GTPase 21 Publication (EC:3.6.5.-Curated)
Alternative name(s):
ATP-binding domain 1 family member B homologBy similarity
Gene namesi
Name:GPN21 Publication
Ordered Locus Names:YOR262WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR262W.
SGDiS000005788. GPN2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi112 – 1121E → K or A: Impairs heterodimer formation with NPA3/GPN1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347GPN-loop GTPase 2PRO_0000245255Add
BLAST

Proteomic databases

MaxQBiQ08726.

Interactioni

Subunit structurei

Heterodimers with NPA3/GPN1 or GPN3 (PubMed:21844196, PubMed:23324351). Binds to RNA polymerase II (RNAPII) (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi34652. 17 interactions.
DIPiDIP-1912N.
IntActiQ08726. 12 interactions.
MINTiMINT-410508.

Structurei

3D structure databases

ProteinModelPortaliQ08726.
SMRiQ08726. Positions 6-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 713Gly-Pro-Asn (GPN)-loop; involved in dimer interfaceBy similarity

Sequence similaritiesi

Belongs to the GPN-loop GTPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075096.
HOGENOMiHOG000207661.
InParanoidiQ08726.
KOiK06883.
OMAiDSHYCAD.
OrthoDBiEOG71VT3Z.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR004130. Gpn.
IPR030231. Gpn2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR21231. PTHR21231. 1 hit.
PTHR21231:SF3. PTHR21231:SF3. 1 hit.
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFAQIVIGP PGSGKSTYCN GCSQFFNAIG RHSQVVNMDP ANDALPYPCA
60 70 80 90 100
VDIRDFITLE EIMQEQQLGP NGGLMYAVES LDNSIDLFIL QIKSLVEEEK
110 120 130 140 150
AYLVFDCPGQ VELFTHHSSL FNIFKKMEKE LDIRFCVVNL IDCFYMTSPS
160 170 180 190 200
QYISILLLAL RSMLMMDLPH INVFSKIDML KSYGELPFRL DYYTEVQDLD
210 220 230 240 250
YLEPYIEKEG SSVLGKKYSK LTETIKELVS DFNLVSFEVL SVDDKESMIN
260 270 280 290 300
LQGVIDKANG YIFGASEVGG DTVWAEASRE GALIANYDIQ DRWIDNKEKY
310 320 330 340
DKEEEEKRTA LLKEQELQNK AVDVNEEDEW ENALKEWEEK QGMDFVR
Length:347
Mass (Da):39,724
Last modified:November 1, 1996 - v1
Checksum:i625CC61455FFA7EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75170 Genomic DNA. Translation: CAA99484.1.
BK006948 Genomic DNA. Translation: DAA11029.1.
PIRiS67159.
RefSeqiNP_014905.1. NM_001183681.1.

Genome annotation databases

EnsemblFungiiYOR262W; YOR262W; YOR262W.
GeneIDi854436.
KEGGisce:YOR262W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75170 Genomic DNA. Translation: CAA99484.1.
BK006948 Genomic DNA. Translation: DAA11029.1.
PIRiS67159.
RefSeqiNP_014905.1. NM_001183681.1.

3D structure databases

ProteinModelPortaliQ08726.
SMRiQ08726. Positions 6-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34652. 17 interactions.
DIPiDIP-1912N.
IntActiQ08726. 12 interactions.
MINTiMINT-410508.

Proteomic databases

MaxQBiQ08726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR262W; YOR262W; YOR262W.
GeneIDi854436.
KEGGisce:YOR262W.

Organism-specific databases

EuPathDBiFungiDB:YOR262W.
SGDiS000005788. GPN2.

Phylogenomic databases

GeneTreeiENSGT00550000075096.
HOGENOMiHOG000207661.
InParanoidiQ08726.
KOiK06883.
OMAiDSHYCAD.
OrthoDBiEOG71VT3Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-33753-MONOMER.

Miscellaneous databases

PROiQ08726.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR004130. Gpn.
IPR030231. Gpn2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR21231. PTHR21231. 1 hit.
PTHR21231:SF3. PTHR21231:SF3. 1 hit.
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1."
    Poirey R., Jauniaux J.-C.
    Yeast 13:483-487(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae."
    Ben-Aroya S., Coombes C., Kwok T., O'Donnell K.A., Boeke J.D., Hieter P.
    Mol. Cell 30:248-258(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A role for GPN-loop GTPase yGPN1 in sister chromatid cohesion."
    Alonso B., Chaussinand G., Armengaud J., Godon C.
    Cell Cycle 10:1828-1837(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  9. "GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein."
    Staresincic L., Walker J., Dirac-Svejstrup A.B., Mitter R., Svejstrup J.Q.
    J. Biol. Chem. 286:35553-35561(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NPA3.
  10. "Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN."
    Alonso B., Beraud C., Meguellati S., Chen S.W., Pellequer J.L., Armengaud J., Godon C.
    Cell Cycle 12:463-472(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NPA3, MUTAGENESIS OF GLU-112.
  11. "Biogenesis of RNA polymerases II and III requires the conserved GPN small GTPases in Saccharomyces cerevisiae."
    Minaker S.W., Filiatrault M.C., Ben-Aroya S., Hieter P., Stirling P.C.
    Genetics 193:853-864(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NPA3 AND GPN3.

Entry informationi

Entry nameiGPN2_YEAST
AccessioniPrimary (citable) accession number: Q08726
Secondary accession number(s): D6W2W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.