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Protein

26S proteasome regulatory subunit RPN8

Gene

RPN8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication

Miscellaneous

Present with 19800 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Enzyme and pathway databases

BioCyciYEAST:G3O-33752-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-382556. ABC-family proteins mediated transport.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM67.973.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN8
Gene namesi
Name:RPN8
Ordered Locus Names:YOR261C
ORF Names:O5360
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR261C.
SGDiS000005787. RPN8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002139512 – 33826S proteasome regulatory subunit RPN8Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei314PhosphoserineCombined sources1
Modified residuei317PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei327PhosphothreonineCombined sources1

Post-translational modificationi

N-acetylated by NAT1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08723.
PaxDbiQ08723.
PRIDEiQ08723.

PTM databases

iPTMnetiQ08723.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34651. 454 interactors.
DIPiDIP-1574N.
IntActiQ08723. 53 interactors.
MINTiQ08723.
STRINGi4932.YOR261C.

Structurei

Secondary structure

1338
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Helixi12 – 24Combined sources13
Beta strandi34 – 40Combined sources7
Beta strandi42 – 53Combined sources12
Beta strandi56 – 58Combined sources3
Beta strandi60 – 62Combined sources3
Beta strandi66 – 68Combined sources3
Helixi70 – 83Combined sources14
Beta strandi87 – 94Combined sources8
Beta strandi96 – 98Combined sources3
Helixi103 – 110Combined sources8
Turni111 – 113Combined sources3
Beta strandi115 – 117Combined sources3
Beta strandi119 – 123Combined sources5
Beta strandi129 – 131Combined sources3
Beta strandi133 – 142Combined sources10
Turni145 – 148Combined sources4
Beta strandi151 – 157Combined sources7
Beta strandi159 – 161Combined sources3
Helixi165 – 175Combined sources11
Helixi186 – 215Combined sources30
Helixi223 – 234Combined sources12
Helixi261 – 306Combined sources46

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J47electron microscopy-U188-308[»]
3JCKelectron microscopy3.50E1-338[»]
3JCOelectron microscopy4.80U1-338[»]
3JCPelectron microscopy4.60U1-338[»]
4CR2electron microscopy7.70U1-338[»]
4CR3electron microscopy9.30U1-338[»]
4CR4electron microscopy8.80U1-338[»]
4O8XX-ray1.99A2-178[»]
4O8YX-ray1.95A2-178[»]
4OCLX-ray2.40A/D1-176[»]
4OCMX-ray1.99A/D1-176[»]
4OCNX-ray2.25A/D1-176[»]
4OWPX-ray2.35A1-186[»]
5A5Belectron microscopy9.50U1-338[»]
5MPBelectron microscopy7.80U1-338[»]
5MPCelectron microscopy7.70U1-338[»]
5MPDelectron microscopy4.10U1-338[»]
5MPEelectron microscopy4.50U1-338[»]
5U4PX-ray2.50A1-177[»]
5W83X-ray1.55A1-338[»]
5WVIelectron microscopy6.30U1-338[»]
5WVKelectron microscopy4.20U1-338[»]
ProteinModelPortaliQ08723.
SMRiQ08723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 143MPNPROSITE-ProRule annotationAdd BLAST136

Sequence similaritiesi

Belongs to the peptidase M67A family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063075.
HOGENOMiHOG000209236.
InParanoidiQ08723.
KOiK03038.
OMAiDAYFAVE.
OrthoDBiEOG092C3ND9.

Family and domain databases

CDDicd08062. MPN_RPN7_8. 1 hit.
InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
IPR037518. MPN.
IPR033858. MPN_RPN7_8.
IPR024969. Rpn11/EIF3F_C.
PANTHERiPTHR10540:SF7. PTHR10540:SF7. 1 hit.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
PROSITEiView protein in PROSITE
PS50249. MPN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLQHEKVTI APLVLLSALD HYERTQTKEN KRCVGVILGD ANSSTIRVTN
60 70 80 90 100
SFALPFEEDE KNSDVWFLDH NYIENMNEMC KKINAKEKLI GWYHSGPKLR
110 120 130 140 150
ASDLKINELF KKYTQNNPLL LIVDVKQQGV GLPTDAYVAI EQVKDDGTST
160 170 180 190 200
EKTFLHLPCT IEAEEAEEIG VEHLLRDVRD QAAGGLSIRL TNQLKSLKGL
210 220 230 240 250
QSKLKDVVEY LDKVINKELP INHTILGKLQ DVFNLLPNLG TPDDDEIDVE
260 270 280 290 300
NHDRINISNN LQKALTVKTN DELMVIYISN LVRSIIAFDD LIENKIQNKK
310 320 330
IQEQRVKDKQ SKVSDDSESE SGDKEATAPL IQRKNKKN
Length:338
Mass (Da):38,313
Last modified:January 23, 2007 - v3
Checksum:i6EE6009545A3D0EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126K → E in AAS56365 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75169 Genomic DNA. Translation: CAA99483.1.
AY558039 Genomic DNA. Translation: AAS56365.1.
BK006948 Genomic DNA. Translation: DAA11028.1.
PIRiS67158.
RefSeqiNP_014904.3. NM_001183680.3.

Genome annotation databases

EnsemblFungiiYOR261C; YOR261C; YOR261C.
GeneIDi854435.
KEGGisce:YOR261C.

Similar proteinsi

Entry informationi

Entry nameiRPN8_YEAST
AccessioniPrimary (citable) accession number: Q08723
Secondary accession number(s): D6W2W2, Q6Q5I7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome