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Q08722 (CD47_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leukocyte surface antigen CD47
Alternative name(s):
Antigenic surface determinant protein OA3
Integrin-associated protein
Short name=IAP
Protein MER6
CD_antigen=CD47
Gene names
Name:CD47
Synonyms:MER6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus By similarity. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. Ref.2 Ref.10 Ref.12

Subunit structure

Interacts with THBS1 and fibrinogen By similarity. Monomer. Interacts with SIRPA, SIRPG, UBQLN1 and UBQLN2. Ref.8 Ref.10 Ref.12

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.2.

Tissue specificity

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Pyrrolidone carboxylic acid
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processblood coagulation

Traceable author statement. Source: Reactome

cell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

extracellular matrix organization

Traceable author statement. Source: Reactome

integrin-mediated signaling pathway

Traceable author statement PubMed 8294396. Source: ProtInc

leukocyte migration

Traceable author statement. Source: Reactome

opsonization

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell activation

Inferred from direct assay Ref.12. Source: UniProtKB

positive regulation of cell proliferation

Inferred from direct assay Ref.12. Source: UniProtKB

positive regulation of cell-cell adhesion

Inferred from direct assay Ref.12. Source: UniProtKB

positive regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

positive regulation of phagocytosis

Inferred from electronic annotation. Source: Ensembl

response to bacterium

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular vesicular exosome

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement Ref.7. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionprotein binding

Inferred from physical interaction Ref.12. Source: UniProtKB

thrombospondin receptor activity

Inferred from physical interaction PubMed 15700281. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SIRPGQ9P1W82EBI-1268321,EBI-1268284

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform OA3-323 (identifier: Q08722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform OA3-293 (identifier: Q08722-2)

The sequence of this isoform differs from the canonical sequence as follows:
     293-323: Missing.
Isoform OA3-305 (identifier: Q08722-3)

The sequence of this isoform differs from the canonical sequence as follows:
     304-305: KA → NN
     306-323: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform OA3-312 (identifier: Q08722-4)

The sequence of this isoform differs from the canonical sequence as follows:
     312-323: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 323305Leukocyte surface antigen CD47
PRO_0000014880

Regions

Topological domain19 – 141123Extracellular Potential
Transmembrane142 – 16221Helical; Potential
Topological domain163 – 17614Cytoplasmic Potential
Transmembrane177 – 19721Helical; Potential
Topological domain198 – 20710Extracellular Potential
Transmembrane208 – 22821Helical; Potential
Topological domain229 – 2357Cytoplasmic Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 26812Extracellular Potential
Transmembrane269 – 28921Helical; Potential
Topological domain290 – 32334Cytoplasmic Potential
Domain19 – 127109Ig-like V-type

Amino acid modifications

Modified residue191Pyrrolidone carboxylic acid
Glycosylation231N-linked (GlcNAc...) Ref.15
Glycosylation341N-linked (GlcNAc...)
Glycosylation501N-linked (GlcNAc...) Ref.14 Ref.15
Glycosylation731N-linked (GlcNAc...) Ref.13 Ref.14 Ref.15
Glycosylation1111N-linked (GlcNAc...) Ref.13 Ref.15
Glycosylation2061N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 263 Ref.9 Ref.15
Disulfide bond41 ↔ 114 Ref.9 Ref.15

Natural variations

Alternative sequence293 – 32331Missing in isoform OA3-293.
VSP_002535
Alternative sequence304 – 3052KA → NN in isoform OA3-305.
VSP_002536
Alternative sequence306 – 32318Missing in isoform OA3-305.
VSP_002537
Alternative sequence312 – 32312Missing in isoform OA3-312.
VSP_002538

Secondary structure

.......................... 323
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform OA3-323 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 5D20730A2632D550

FASTA32335,214
        10         20         30         40         50         60 
MWPLVAALLL GSACCGSAQL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN TTEVYVKWKF 

        70         80         90        100        110        120 
KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM DKSDAVSHTG NYTCEVTELT 

       130        140        150        160        170        180 
REGETIIELK YRVVSWFSPN ENILIVIFPI FAILLFWGQF GIKTLKYRSG GMDEKTIALL 

       190        200        210        220        230        240 
VAGLVITVIV IVGAILFVPG EYSLKNATGL GLIVTSTGIL ILLHYYVFST AIGLTSFVIA 

       250        260        270        280        290        300 
ILVIQVIAYI LAVVGLSLCI AACIPMHGPL LISGLSILAL AQLLGLVYMK FVASNQKTIQ 

       310        320 
PPRKAVEEPL NAFKESKGMM NDE 

« Hide

Isoform OA3-293 [UniParc].

Checksum: 81A2F6696D11F4E1
Show »

FASTA29231,743
Isoform OA3-305 [UniParc].

Checksum: 145DD178068F8DBD
Show »

FASTA30533,192
Isoform OA3-312 [UniParc].

Checksum: 8BBBB21A590D4DCA
Show »

FASTA31133,845

References

« Hide 'large scale' references
[1]"An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains."
Campbell I.G., Freemont P.S., Foulkes W., Trowsdale J.
Cancer Res. 52:5416-5420(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM OA3-323).
Tissue: Ovary.
[2]"Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane spanning domains implicated in alpha-v beta-3-dependent ligand binding."
Lindberg F.P., Gresham H.D., Schwarz E., Brown E.J.
J. Cell Biol. 123:485-496(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM OA3-305), FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
Tissue: Myelomonocyte.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-305).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-323).
Tissue: Brain.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-305).
Tissue: Hippocampus, Leiomyosarcoma and Ovarian adenocarcinoma.
[7]"Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3."
Mawby W.J., Holmes C.H., Anstee D.J., Spring F.A., Tanner M.J.A.
Biochem. J. 304:525-530(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION AS CD47.
Tissue: Erythrocyte.
[8]"Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane."
Wu A.-L., Wang J., Zheleznyak A., Brown E.J.
Mol. Cell 4:619-625(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBQLN1 AND UBQLN2.
[9]"Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains."
Rebres R.A., Vaz L.E., Green J.M., Brown E.J.
J. Biol. Chem. 276:34607-34616(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BOND.
[10]"Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation."
Latour S., Tanaka H., Demeure C., Mateo V., Rubio M., Brown E.J., Maliszewski C., Lindberg F.P., Oldenborg A., Ullrich A., Delespesse G., Sarfati M.
J. Immunol. 167:2547-2554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SIRPA.
[11]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[12]"Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation."
Piccio L., Vermi W., Boles K.S., Fuchs A., Strader C.A., Facchetti F., Cella M., Colonna M.
Blood 105:2421-2427(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SIRPG.
Tissue: T-cell.
[13]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-73 AND ASN-111.
Tissue: Liver.
[14]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-50 AND ASN-73.
Tissue: Leukemic T-cell.
[15]"Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47."
Hatherley D., Graham S.C., Turner J., Harlos K., Stuart D.I., Barclay A.N.
Mol. Cell 31:266-277(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 19-136 IN COMPLEX WITH SIRPA, DISULFIDE BOND, GLYCOSYLATION AT ASN-23; ASN-50; ASN-73 AND ASN-111, PYROGLUTAMATE FORMATION AT GLN-19.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69398 mRNA. Translation: CAA49196.1.
Z25521 mRNA. Translation: CAA80977.1.
BT006907 mRNA. Translation: AAP35553.1.
AK289813 mRNA. Translation: BAF82502.1.
CH471052 Genomic DNA. Translation: EAW79733.1.
CH471052 Genomic DNA. Translation: EAW79734.1.
BC010016 mRNA. Translation: AAH10016.1.
BC012884 mRNA. Translation: AAH12884.1.
BC037306 mRNA. Translation: AAH37306.1.
CCDSCCDS43125.1. [Q08722-3]
CCDS43126.1. [Q08722-1]
PIRA48997.
RefSeqNP_001768.1. NM_001777.3. [Q08722-1]
NP_942088.1. NM_198793.2. [Q08722-3]
UniGeneHs.446414.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JJSX-ray1.85C/D20-136[»]
2JJTX-ray2.30C/D20-136[»]
2VSCX-ray1.90A/B/C/D20-136[»]
4CMMX-ray1.92B20-136[»]
4KJYX-ray1.93A/C19-135[»]
ProteinModelPortalQ08722.
SMRQ08722. Positions 19-134.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107399. 5 interactions.
IntActQ08722. 2 interactions.
STRING9606.ENSP00000355361.

PTM databases

PhosphoSiteQ08722.

Polymorphism databases

DMDM1171879.

Proteomic databases

MaxQBQ08722.
PaxDbQ08722.
PRIDEQ08722.

Protocols and materials databases

DNASU961.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000355354; ENSP00000347512; ENSG00000196776. [Q08722-3]
ENST00000361309; ENSP00000355361; ENSG00000196776. [Q08722-1]
GeneID961.
KEGGhsa:961.
UCSCuc003dwt.1. human. [Q08722-1]
uc003dwv.1. human. [Q08722-3]

Organism-specific databases

CTD961.
GeneCardsGC03M107761.
HGNCHGNC:1682. CD47.
HPACAB016055.
HPA044659.
MIM601028. gene.
neXtProtNX_Q08722.
PharmGKBPA26222.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42016.
HOGENOMHOG000013020.
HOVERGENHBG003808.
KOK06266.
OMAVYMKFVA.
OrthoDBEOG79GT78.
PhylomeDBQ08722.
TreeFamTF336026.

Enzyme and pathway databases

ReactomeREACT_111155. Cell-Cell communication.
REACT_118779. Extracellular matrix organization.
REACT_604. Hemostasis.

Gene expression databases

BgeeQ08722.
CleanExHS_CD47.
GenevestigatorQ08722.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR006704. CD47.
IPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10613. PTHR10613. 1 hit.
PfamPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD47. human.
EvolutionaryTraceQ08722.
GeneWikiCD47.
GenomeRNAi961.
NextBio4012.
PROQ08722.
SOURCESearch...

Entry information

Entry nameCD47_HUMAN
AccessionPrimary (citable) accession number: Q08722
Secondary accession number(s): A8K198 expand/collapse secondary AC list , D3DN59, Q53Y71, Q96A60
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries