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Protein

Leukocyte surface antigen CD47

Gene

CD47

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.By similarity3 Publications

GO - Molecular functioni

  • thrombospondin receptor activity Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-216083. Integrin cell surface interactions.
R-HSA-391160. Signal regulatory protein (SIRP) family interactions.
R-HSA-6798695. Neutrophil degranulation.
SIGNORiQ08722.

Protein family/group databases

TCDBi1.N.1.1.1. the osteoclast fusion complex (ofc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukocyte surface antigen CD47
Alternative name(s):
Antigenic surface determinant protein OA3
Integrin-associated protein
Short name:
IAP
Protein MER6
CD_antigen: CD47
Gene namesi
Name:CD47
Synonyms:MER6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:1682. CD47.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 141ExtracellularSequence analysisAdd BLAST123
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Topological domaini163 – 176CytoplasmicSequence analysisAdd BLAST14
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 207ExtracellularSequence analysis10
Transmembranei208 – 228HelicalSequence analysisAdd BLAST21
Topological domaini229 – 235CytoplasmicSequence analysis7
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 268ExtracellularSequence analysisAdd BLAST12
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290 – 323CytoplasmicSequence analysisAdd BLAST34

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi961.
OpenTargetsiENSG00000196776.
PharmGKBiPA26222.

Polymorphism and mutation databases

BioMutaiCD47.
DMDMi1171879.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001488019 – 323Leukocyte surface antigen CD47Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acid1 Publication1
Glycosylationi23N-linked (GlcNAc...)1 Publication1
Disulfide bondi33 ↔ 2631 Publication
Glycosylationi34N-linked (GlcNAc...)1
Disulfide bondi41 ↔ 1141 Publication
Glycosylationi50N-linked (GlcNAc...)2 Publications1
Glycosylationi73N-linked (GlcNAc...)3 Publications1
Modified residuei89PhosphoserineBy similarity1
Glycosylationi111N-linked (GlcNAc...)2 Publications1
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

EPDiQ08722.
MaxQBiQ08722.
PaxDbiQ08722.
PeptideAtlasiQ08722.
PRIDEiQ08722.

PTM databases

iPTMnetiQ08722.
PhosphoSitePlusiQ08722.
SwissPalmiQ08722.

Expressioni

Tissue specificityi

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Gene expression databases

BgeeiENSG00000196776.
CleanExiHS_CD47.
ExpressionAtlasiQ08722. baseline and differential.
GenevisibleiQ08722. HS.

Organism-specific databases

HPAiCAB016055.
HPA044659.

Interactioni

Subunit structurei

Interacts with THBS1 and fibrinogen (By similarity). Monomer. Interacts with SIRPA, SIRPG, UBQLN1 and UBQLN2.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SIRPGQ9P1W82EBI-1268321,EBI-1268284
SYNE4Q8N2053EBI-1268321,EBI-7131783

Protein-protein interaction databases

BioGridi107399. 19 interactors.
DIPiDIP-39948N.
IntActiQ08722. 4 interactors.
STRINGi9606.ENSP00000355361.

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 30Combined sources5
Beta strandi35 – 39Combined sources5
Beta strandi42 – 45Combined sources4
Helixi51 – 53Combined sources3
Beta strandi54 – 60Combined sources7
Beta strandi63 – 69Combined sources7
Helixi70 – 72Combined sources3
Beta strandi74 – 76Combined sources3
Helixi79 – 81Combined sources3
Helixi88 – 93Combined sources6
Beta strandi98 – 101Combined sources4
Helixi102 – 106Combined sources5
Beta strandi110 – 118Combined sources9
Beta strandi121 – 131Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JJSX-ray1.85C/D20-136[»]
2JJTX-ray2.30C/D20-136[»]
2VSCX-ray1.90A/B/C/D20-136[»]
4CMMX-ray1.92B20-136[»]
4KJYX-ray1.93A/C19-135[»]
5IWLX-ray2.80C/D20-132[»]
ProteinModelPortaliQ08722.
SMRiQ08722.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08722.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 127Ig-like V-typeAdd BLAST109

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJTS. Eukaryota.
ENOG41113GI. LUCA.
GeneTreeiENSGT00390000007697.
HOGENOMiHOG000013020.
HOVERGENiHBG003808.
InParanoidiQ08722.
KOiK06266.
OMAiVYMKFVA.
OrthoDBiEOG091G0HUR.
PhylomeDBiQ08722.
TreeFamiTF336026.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR006704. CD47.
IPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10613. PTHR10613. 1 hit.
PfamiPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform OA3-323 (identifier: Q08722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWPLVAALLL GSACCGSAQL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN
60 70 80 90 100
TTEVYVKWKF KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM
110 120 130 140 150
DKSDAVSHTG NYTCEVTELT REGETIIELK YRVVSWFSPN ENILIVIFPI
160 170 180 190 200
FAILLFWGQF GIKTLKYRSG GMDEKTIALL VAGLVITVIV IVGAILFVPG
210 220 230 240 250
EYSLKNATGL GLIVTSTGIL ILLHYYVFST AIGLTSFVIA ILVIQVIAYI
260 270 280 290 300
LAVVGLSLCI AACIPMHGPL LISGLSILAL AQLLGLVYMK FVASNQKTIQ
310 320
PPRKAVEEPL NAFKESKGMM NDE
Length:323
Mass (Da):35,214
Last modified:November 1, 1995 - v1
Checksum:i5D20730A2632D550
GO
Isoform OA3-293 (identifier: Q08722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     293-323: Missing.

Show »
Length:292
Mass (Da):31,743
Checksum:i81A2F6696D11F4E1
GO
Isoform OA3-305 (identifier: Q08722-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-305: KA → NN
     306-323: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:305
Mass (Da):33,192
Checksum:i145DD178068F8DBD
GO
Isoform OA3-312 (identifier: Q08722-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-323: Missing.

Show »
Length:311
Mass (Da):33,845
Checksum:i8BBBB21A590D4DCA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002535293 – 323Missing in isoform OA3-293. CuratedAdd BLAST31
Alternative sequenceiVSP_002536304 – 305KA → NN in isoform OA3-305. 3 Publications2
Alternative sequenceiVSP_002537306 – 323Missing in isoform OA3-305. 3 PublicationsAdd BLAST18
Alternative sequenceiVSP_002538312 – 323Missing in isoform OA3-312. CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69398 mRNA. Translation: CAA49196.1.
Z25521 mRNA. Translation: CAA80977.1.
BT006907 mRNA. Translation: AAP35553.1.
AK289813 mRNA. Translation: BAF82502.1.
CH471052 Genomic DNA. Translation: EAW79733.1.
CH471052 Genomic DNA. Translation: EAW79734.1.
BC010016 mRNA. Translation: AAH10016.1.
BC012884 mRNA. Translation: AAH12884.1.
BC037306 mRNA. Translation: AAH37306.1.
CCDSiCCDS43125.1. [Q08722-3]
CCDS43126.1. [Q08722-1]
PIRiA48997.
RefSeqiNP_001768.1. NM_001777.3. [Q08722-1]
NP_942088.1. NM_198793.2. [Q08722-3]
UniGeneiHs.446414.

Genome annotation databases

EnsembliENST00000355354; ENSP00000347512; ENSG00000196776. [Q08722-3]
ENST00000361309; ENSP00000355361; ENSG00000196776. [Q08722-1]
GeneIDi961.
KEGGihsa:961.
UCSCiuc003dwt.2. human. [Q08722-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69398 mRNA. Translation: CAA49196.1.
Z25521 mRNA. Translation: CAA80977.1.
BT006907 mRNA. Translation: AAP35553.1.
AK289813 mRNA. Translation: BAF82502.1.
CH471052 Genomic DNA. Translation: EAW79733.1.
CH471052 Genomic DNA. Translation: EAW79734.1.
BC010016 mRNA. Translation: AAH10016.1.
BC012884 mRNA. Translation: AAH12884.1.
BC037306 mRNA. Translation: AAH37306.1.
CCDSiCCDS43125.1. [Q08722-3]
CCDS43126.1. [Q08722-1]
PIRiA48997.
RefSeqiNP_001768.1. NM_001777.3. [Q08722-1]
NP_942088.1. NM_198793.2. [Q08722-3]
UniGeneiHs.446414.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JJSX-ray1.85C/D20-136[»]
2JJTX-ray2.30C/D20-136[»]
2VSCX-ray1.90A/B/C/D20-136[»]
4CMMX-ray1.92B20-136[»]
4KJYX-ray1.93A/C19-135[»]
5IWLX-ray2.80C/D20-132[»]
ProteinModelPortaliQ08722.
SMRiQ08722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107399. 19 interactors.
DIPiDIP-39948N.
IntActiQ08722. 4 interactors.
STRINGi9606.ENSP00000355361.

Protein family/group databases

TCDBi1.N.1.1.1. the osteoclast fusion complex (ofc) family.

PTM databases

iPTMnetiQ08722.
PhosphoSitePlusiQ08722.
SwissPalmiQ08722.

Polymorphism and mutation databases

BioMutaiCD47.
DMDMi1171879.

Proteomic databases

EPDiQ08722.
MaxQBiQ08722.
PaxDbiQ08722.
PeptideAtlasiQ08722.
PRIDEiQ08722.

Protocols and materials databases

DNASUi961.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355354; ENSP00000347512; ENSG00000196776. [Q08722-3]
ENST00000361309; ENSP00000355361; ENSG00000196776. [Q08722-1]
GeneIDi961.
KEGGihsa:961.
UCSCiuc003dwt.2. human. [Q08722-1]

Organism-specific databases

CTDi961.
DisGeNETi961.
GeneCardsiCD47.
HGNCiHGNC:1682. CD47.
HPAiCAB016055.
HPA044659.
MIMi601028. gene.
neXtProtiNX_Q08722.
OpenTargetsiENSG00000196776.
PharmGKBiPA26222.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJTS. Eukaryota.
ENOG41113GI. LUCA.
GeneTreeiENSGT00390000007697.
HOGENOMiHOG000013020.
HOVERGENiHBG003808.
InParanoidiQ08722.
KOiK06266.
OMAiVYMKFVA.
OrthoDBiEOG091G0HUR.
PhylomeDBiQ08722.
TreeFamiTF336026.

Enzyme and pathway databases

ReactomeiR-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-216083. Integrin cell surface interactions.
R-HSA-391160. Signal regulatory protein (SIRP) family interactions.
R-HSA-6798695. Neutrophil degranulation.
SIGNORiQ08722.

Miscellaneous databases

ChiTaRSiCD47. human.
EvolutionaryTraceiQ08722.
GeneWikiiCD47.
GenomeRNAii961.
PROiQ08722.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196776.
CleanExiHS_CD47.
ExpressionAtlasiQ08722. baseline and differential.
GenevisibleiQ08722. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR006704. CD47.
IPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10613. PTHR10613. 1 hit.
PfamiPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD47_HUMAN
AccessioniPrimary (citable) accession number: Q08722
Secondary accession number(s): A8K198
, D3DN59, Q53Y71, Q96A60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.