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Reviewed, UniProtKB/Swiss-Prot Q08722 (CD47_HUMAN)

Last modified November 25, 2008. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Leukocyte surface antigen CD47
Alternative name(s):
    Integrin-associated protein
      Short name=IAP
    Antigenic surface determinant protein OA3
    Protein MER6
    CD_antigen=CD47
Gene names
Name: CD47
Synonyms: MER6
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus By similarity. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Subunit structure

Interacts with THBS1 and fibrinogen By similarity. Monomer. Interacts with SIRPA, SIRPG, UBQLN1 and UBQLN2.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform OA3-323 (identifier: Q08722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform OA3-293 (identifier: Q08722-2)

The sequence of this isoform differs from the canonical sequence as follows:
     293-323: Missing.
Isoform OA3-305 (identifier: Q08722-3)

The sequence of this isoform differs from the canonical sequence as follows:
     304-305: KA → NN
     306-323: Missing.
Notes: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform OA3-312 (identifier: Q08722-4)

The sequence of this isoform differs from the canonical sequence as follows:
     312-323: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 323305Leukocyte surface antigen CD47
PRO_0000014880

Regions

Topological domain19 – 141123Extracellular Potential
Transmembrane142 – 16221 Potential
Topological domain163 – 17614Cytoplasmic Potential
Transmembrane177 – 19721 Potential
Topological domain198 – 20710Extracellular Potential
Transmembrane208 – 22821 Potential
Topological domain229 – 2357Cytoplasmic Potential
Transmembrane236 – 25621 Potential
Topological domain257 – 26812Extracellular Potential
Transmembrane269 – 28921 Potential
Topological domain290 – 32334Cytoplasmic Potential
Domain19 – 127109Ig-like V-type

Amino acid modifications

Modified residue191Pyrrolidone carboxylic acid Probable
Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...)
Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation731N-linked (GlcNAc...)
Glycosylation1111N-linked (GlcNAc...)
Glycosylation2061N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 263
Disulfide bond41 ↔ 114 By similarity

Natural variations

Alternative sequence293 – 32331Missing in isoform OA3-293.
VSP_002535
Alternative sequence304 – 3052KA → NN in isoform OA3-305.
VSP_002536
Alternative sequence306 – 32318Missing in isoform OA3-305.
VSP_002537
Alternative sequence312 – 32312Missing in isoform OA3-312.
VSP_002538

Sequences

Sequence LengthMass (Da)Tools
Isoform OA3-323 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 5D20730A2632D550

FASTA32335,214
        10         20         30         40         50         60 
MWPLVAALLL GSACCGSAQL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN TTEVYVKWKF 

        70         80         90        100        110        120 
KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM DKSDAVSHTG NYTCEVTELT 

       130        140        150        160        170        180 
REGETIIELK YRVVSWFSPN ENILIVIFPI FAILLFWGQF GIKTLKYRSG GMDEKTIALL 

       190        200        210        220        230        240 
VAGLVITVIV IVGAILFVPG EYSLKNATGL GLIVTSTGIL ILLHYYVFST AIGLTSFVIA 

       250        260        270        280        290        300 
ILVIQVIAYI LAVVGLSLCI AACIPMHGPL LISGLSILAL AQLLGLVYMK FVASNQKTIQ 

       310        320 
PPRKAVEEPL NAFKESKGMM NDE 

« Hide

Isoform OA3-293 [UniParc].

Checksum: 81A2F6696D11F4E1
Show »

29231,743
Isoform OA3-305 [UniParc].

Checksum: 145DD178068F8DBD
Show »

30533,192
Isoform OA3-312 [UniParc].

Checksum: 8BBBB21A590D4DCA
Show »

31133,845

References

« Hide 'large scale' references
[1]"An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains."
Campbell I.G., Freemont P.S., Foulkes W., Trowsdale J.
Cancer Res. 52:5416-5420(1992) [PubMed: 1394148] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM OA3-323).
Tissue: Ovary.
[2]"Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane spanning domains implicated in alpha-v beta-3-dependent ligand binding."
Lindberg F.P., Gresham H.D., Schwarz E., Brown E.J.
J. Cell Biol. 123:485-496(1993) [PubMed: 7691831] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM OA3-305), FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
Tissue: Myelomonocyte.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-305).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-305).
Tissue: Hippocampus, Leiomyosarcoma and Ovarian adenocarcinoma.
[5]"Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3."
Mawby W.J., Holmes C.H., Anstee D.J., Spring F.A., Tanner M.J.A.
Biochem. J. 304:525-530(1994) [PubMed: 7998989] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION AS CD47.
Tissue: Erythrocyte.
[6]"Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane."
Wu A.-L., Wang J., Zheleznyak A., Brown E.J.
Mol. Cell 4:619-625(1999) [PubMed: 10549293] [Abstract]
Cited for: INTERACTION WITH UBQLN1 AND UBQLN2.
[7]"Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains."
Rebres R.A., Vaz L.E., Green J.M., Brown E.J.
J. Biol. Chem. 276:34607-34616(2001) [PubMed: 11454874] [Abstract]
Cited for: DISULFIDE BOND.
[8]"Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation."
Latour S., Tanaka H., Demeure C., Mateo V., Rubio M., Brown E.J., Maliszewski C., Lindberg F.P., Oldenborg A., Ullrich A., Delespesse G., Sarfati M.
J. Immunol. 167:2547-2554(2001) [PubMed: 11509594] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SIRPA.
[9]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[10]"Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation."
Piccio L., Vermi W., Boles K.S., Fuchs A., Strader C.A., Facchetti F., Cella M., Colonna M.
Blood 105:2421-2427(2005) [PubMed: 15383453] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SIRPG.
Tissue: T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

X69398 mRNA. Translation: CAA49196.1.
Z25521 mRNA. Translation: CAA80977.1.
BT006907 mRNA. Translation: AAP35553.1.
BC010016 mRNA. Translation: AAH10016.1.
BC012884 mRNA. Translation: AAH12884.1.
BC037306 mRNA. Translation: AAH37306.1.
PIRA48997.
RefSeqNP_001020250.1.
NP_001768.1.
NP_942088.1.
UniGeneHs.446414

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2JJSX-ray1.85C/D19-136[»]
2JJTX-ray2.30C/D19-136[»]
2VSCX-ray1.90A/B/C/D19-136[»]
ModBaseSearch...

Protein-protein interaction databases

IntActQ08722.

Genome annotation databases

EnsemblENSG00000196776. Homo sapiens. [Contig view]
GeneID961.
KEGGhsa:961.

Organism-specific databases

H-InvDBHIX0003524.
HGNCHGNC:1682. CD47.
HPACAB016055.
MIM601028. gene.
PharmGKBPA26222.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ08722.
HOVERGENQ08722.

Gene expression databases

ArrayExpressQ08722.
CleanExHS_CD47.
GermOnlineENSG00000196776. Homo sapiens.

Family and domain databases

InterProIPR006704. CD47.
IPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like.
[Graphical view]
PANTHERPTHR10613. CD47. 1 hit.
PfamPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubQ08722.
NextBio4012.
SOURCESearch...

Entry information

Entry nameCD47_HUMAN
AccessionPrimary (citable) accession number: Q08722
Secondary accession number(s): Q53Y71, Q96A60
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 25, 2008
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents