Q08704 (CFI_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone--flavonone isomerase
Short name=Chalcone isomerase
EC=5.5.1.6

Gene names

Name:	CHI
Synonyms:	CHI1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Tissue specificity	Pericarp. Ref.1
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	cellular modified amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Chalcone--flavonone isomerase		PRO_0000166433
Sites
	Binding site	46	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity
	Site	104	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08704 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: AD64A8B04213F9F5	FASTA	231	24,250
	10 20 30 40 50 60 MACRRWWSTA VVFPPVARPP GSAGSHFLGG AGVRGVEIGG NFIKFTAIGV YLEDAAVPAL 70 80 90 100 110 120 AKKWGGKTAD ELASDAAFFR DVVTGDFEKF TRVTMILPLT GEQYAEKVTE NCVAFWKAAG 130 140 150 160 170 180 LYTDAEGVAV EKFREVFKPE TFAPGRSILF THSPAGVLTV AFSKDSSVPA AGGVAIENKR 190 200 210 220 230 LCEAVLESII GERGVSPAAK LSLAARVSEL LAKETAAAAD APQAEPVSIT A

References

[1]	"Isolation and characterization of a maize gene encoding chalcone flavonone isomerase." Grotewold E., Peterson T. Mol. Gen. Genet. 242:1-8(1994) [PubMed: 8277939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22760 Genomic DNA. Translation: CAA80441.1.
PIR	S41570.
3D structure databases
ProteinModelPortal	Q08704.
SMR	Q08704. Positions 10-213.
ModBase	Search...
Proteomic databases
PRIDE	Q08704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q08704.
MaizeGDB	56296.
Phylogenomic databases
GeneTree	EPGT00070000029529.
Family and domain databases
InterPro	IPR016087. Chalcone_isomerase. IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
SUPFAM	SSF54626. Chalcone. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CFI_MAIZE

Accession

Primary (citable) accession number: Q08704

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program