Reviewed, UniProtKB/Swiss-Prot Q08704 (CFI_MAIZE)

Names and origin

Protein names

Recommended name:
    Chalcone--flavonone isomerase
      Short name=Chalcone isomerase
    EC=5.5.1.6

Gene names

Name:	CHI
Synonyms:	CHI1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Tissue specificity	Pericarp. Ref.1
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Chalcone--flavonone isomerase		PRO_0000166433
Sites
	Binding site	46	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity
	Site	104	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08704-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: AD64A8B04213F9F5	FASTA	231	24,250
	10 20 30 40 50 60 MACRRWWSTA VVFPPVARPP GSAGSHFLGG AGVRGVEIGG NFIKFTAIGV YLEDAAVPAL 70 80 90 100 110 120 AKKWGGKTAD ELASDAAFFR DVVTGDFEKF TRVTMILPLT GEQYAEKVTE NCVAFWKAAG 130 140 150 160 170 180 LYTDAEGVAV EKFREVFKPE TFAPGRSILF THSPAGVLTV AFSKDSSVPA AGGVAIENKR 190 200 210 220 230 LCEAVLESII GERGVSPAAK LSLAARVSEL LAKETAAAAD APQAEPVSIT A

References

[1]	"Isolation and characterization of a maize gene encoding chalcone flavonone isomerase." Grotewold E., Peterson T. Mol. Gen. Genet. 242:1-8(1994) [PubMed: 8277939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22760 Genomic DNA. Translation: CAA80441.1.
PIR	S41570.
3D structure databases
HSSP	HSSP built from PDB template 1EYQ based on UniProtKB P28012.
ModBase	Search...
Organism-specific databases
Gramene	Q08704.
MaizeGDB	56296.
Enzyme and pathway databases
BRENDA	5.5.1.6. 289.
Family and domain databases
InterPro	IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CFI_MAIZE

Accession

Primary (citable) accession number: Q08704

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)