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Protein

Aprataxin-like protein

Gene

HNT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241, PubMed:24362567). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).By similarity2 Publications

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Adenosine-5'-diphospho-5'-(DNA) + H2O = AMP + phospho-5'-(DNA).2 Publications
Adenosine-5'-diphospho-5'-(ribonucleotide)-(DNA) + H2O = AMP + 5'-phospho-(ribonucleotide)-(DNA).2 Publications
(DNA)-3'-diphospho-5'-guanosine + H2O = (DNA)-3'-phosphate + GMP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130NucleophileBy similarity1
Metal bindingi188ZincBy similarity1
Metal bindingi191ZincBy similarity1
Metal bindingi205Zinc; via tele nitrogenBy similarity1
Metal bindingi209ZincBy similarity1

GO - Molecular functioni

  • DNA 5'-adenosine monophosphate hydrolase activity Source: UniProtKB
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Hydrolase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33749-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aprataxin-like protein (EC:3.1.11.72 Publications, EC:3.1.12.2By similarity)
Alternative name(s):
Hit family protein 3
Gene namesi
Name:HNT3
Ordered Locus Names:YOR258W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR258W.
SGDiS000005784. HNT3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098021 – 217Aprataxin-like proteinAdd BLAST217

Proteomic databases

MaxQBiQ08702.
PRIDEiQ08702.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei10Interaction with DNABy similarity1

Protein-protein interaction databases

BioGridi34648. 21 interactors.
DIPiDIP-5388N.
IntActiQ08702. 11 interactors.
MINTiMINT-518216.
STRINGi4932.YOR258W.

Structurei

3D structure databases

ProteinModelPortaliQ08702.
SMRiQ08702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 139HITAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 38Interaction with DNABy similarity5
Regioni121 – 132Interaction with DNABy similarityAdd BLAST12
Regioni144 – 148Interaction with DNABy similarity5

Domaini

The HIT domain is required for enzymatic activity.By similarity

Phylogenomic databases

GeneTreeiENSGT00570000079163.
HOGENOMiHOG000248858.
InParanoidiQ08702.
KOiK10863.
OMAiIHAHPSM.
OrthoDBiEOG092C3DRA.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiView protein in InterPro
IPR026963. Aprataxin.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
PANTHERiPTHR12486:SF7. PTHR12486:SF7. 1 hit.
PfamiView protein in Pfam
PF01230. HIT. 1 hit.
PF16278. zf-C2HE. 1 hit.
SUPFAMiSSF54197. SSF54197. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWRYALKNY VTSPETVNDD TVTYFDDKVS IIRDSFPKSE CHLLILPRTM
60 70 80 90 100
QLSRSHPTKV IDAKFKNEFE SYVNSAIDHI FRHFQEKFRI KKSDDDKDPC
110 120 130 140 150
WDDILKDKNK FVRNFVQVGI HSVPSMANLH IHVISKDFHS VRLKNKKHYN
160 170 180 190 200
SFNTGFFISW DDLPLNGKNL GTDKEIETTY LKEHDLLCCY CQRNFSNKFS
210
LLKKHLELEF NSHFELK
Length:217
Mass (Da):25,757
Last modified:November 1, 1996 - v1
Checksum:i8BA406AEDF17FA29
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80I → S in AAA66894 (PubMed:3526331).Curated1
Sequence conflicti157F → N in AAA66894 (PubMed:3526331).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14078 Genomic DNA. Translation: AAA66894.1.
Z75166 Genomic DNA. Translation: CAA99480.1.
BK006948 Genomic DNA. Translation: DAA11025.1.
PIRiS67155.
RefSeqiNP_014901.1. NM_001183677.1.

Genome annotation databases

EnsemblFungiiYOR258W; YOR258W; YOR258W.
GeneIDi854432.
KEGGisce:YOR258W.

Similar proteinsi

Entry informationi

Entry nameiAPTX_YEAST
AccessioniPrimary (citable) accession number: Q08702
Secondary accession number(s): D6W2V9, Q05189
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names