Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aprataxin-like protein

Gene

HNT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined.1 Publication

GO - Molecular functioni

  • DNA 5'-adenosine monophosphate hydrolase activity Source: UniProtKB
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33749-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aprataxin-like protein (EC:3.-.-.-)
Alternative name(s):
Hit family protein 3
Gene namesi
Name:HNT3
Ordered Locus Names:YOR258W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR258W.
SGDiS000005784. HNT3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098021 – 217Aprataxin-like proteinAdd BLAST217

Proteomic databases

MaxQBiQ08702.
PRIDEiQ08702.

Interactioni

Protein-protein interaction databases

BioGridi34648. 20 interactors.
DIPiDIP-5388N.
IntActiQ08702. 2 interactors.
MINTiMINT-518216.

Structurei

3D structure databases

ProteinModelPortaliQ08702.
SMRiQ08702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 139HITAdd BLAST134

Domaini

The HIT domain is required for enzymatic activity.By similarity

Sequence similaritiesi

Contains 1 HIT domain.Curated

Phylogenomic databases

GeneTreeiENSGT00570000079163.
HOGENOMiHOG000248858.
InParanoidiQ08702.
KOiK10863.
OMAiHYNSFST.
OrthoDBiEOG092C3DRA.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR026963. Aprataxin.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
[Graphical view]
PANTHERiPTHR12486:SF4. PTHR12486:SF4. 2 hits.
PfamiPF01230. HIT. 1 hit.
PF16278. zf-C2HE. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWRYALKNY VTSPETVNDD TVTYFDDKVS IIRDSFPKSE CHLLILPRTM
60 70 80 90 100
QLSRSHPTKV IDAKFKNEFE SYVNSAIDHI FRHFQEKFRI KKSDDDKDPC
110 120 130 140 150
WDDILKDKNK FVRNFVQVGI HSVPSMANLH IHVISKDFHS VRLKNKKHYN
160 170 180 190 200
SFNTGFFISW DDLPLNGKNL GTDKEIETTY LKEHDLLCCY CQRNFSNKFS
210
LLKKHLELEF NSHFELK
Length:217
Mass (Da):25,757
Last modified:November 1, 1996 - v1
Checksum:i8BA406AEDF17FA29
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80I → S in AAA66894 (PubMed:3526331).Curated1
Sequence conflicti157F → N in AAA66894 (PubMed:3526331).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14078 Genomic DNA. Translation: AAA66894.1.
Z75166 Genomic DNA. Translation: CAA99480.1.
BK006948 Genomic DNA. Translation: DAA11025.1.
PIRiS67155.
RefSeqiNP_014901.1. NM_001183677.1.

Genome annotation databases

EnsemblFungiiYOR258W; YOR258W; YOR258W.
GeneIDi854432.
KEGGisce:YOR258W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14078 Genomic DNA. Translation: AAA66894.1.
Z75166 Genomic DNA. Translation: CAA99480.1.
BK006948 Genomic DNA. Translation: DAA11025.1.
PIRiS67155.
RefSeqiNP_014901.1. NM_001183677.1.

3D structure databases

ProteinModelPortaliQ08702.
SMRiQ08702.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34648. 20 interactors.
DIPiDIP-5388N.
IntActiQ08702. 2 interactors.
MINTiMINT-518216.

Proteomic databases

MaxQBiQ08702.
PRIDEiQ08702.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR258W; YOR258W; YOR258W.
GeneIDi854432.
KEGGisce:YOR258W.

Organism-specific databases

EuPathDBiFungiDB:YOR258W.
SGDiS000005784. HNT3.

Phylogenomic databases

GeneTreeiENSGT00570000079163.
HOGENOMiHOG000248858.
InParanoidiQ08702.
KOiK10863.
OMAiHYNSFST.
OrthoDBiEOG092C3DRA.

Enzyme and pathway databases

BioCyciYEAST:G3O-33749-MONOMER.

Miscellaneous databases

PROiQ08702.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR026963. Aprataxin.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
[Graphical view]
PANTHERiPTHR12486:SF4. PTHR12486:SF4. 2 hits.
PfamiPF01230. HIT. 1 hit.
PF16278. zf-C2HE. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPTX_YEAST
AccessioniPrimary (citable) accession number: Q08702
Secondary accession number(s): D6W2V9, Q05189
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.