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Protein

N-alpha-acetyltransferase NAT5

Gene

NAT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine (PubMed:25886145). Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides (PubMed:25886145). Non-essential component of the NatA N-terminal acetyltransferase (PubMed:14517307).2 Publications

Miscellaneous

Present with 2370 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-seryl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-seryl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-valyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-threonyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-threonyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-lysyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-tyrosyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA.1 Publication

GO - Molecular functioni

  • peptide alpha-N-acetyltransferase activity Source: SGD

GO - Biological processi

  • N-terminal protein amino acid acetylation Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33744-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase NAT5Curated (EC:2.3.1.2581 Publication)
Short name:
NatA complex subunit NAT5
Gene namesi
Name:NAT51 PublicationImported
Ordered Locus Names:YOR253W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR253W.
SGDiS000005779. NAT5.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002406421 – 176N-alpha-acetyltransferase NAT5Add BLAST176

Proteomic databases

MaxQBiQ08689.
PRIDEiQ08689.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase A (NatA) complex, which is composed of ARD1, NAT1 and NAT5.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34643. 44 interactors.
IntActiQ08689. 11 interactors.
MINTiMINT-2782295.
STRINGi4932.YOR253W.

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi13 – 15Combined sources3
Helixi16 – 26Combined sources11
Helixi33 – 39Combined sources7
Beta strandi59 – 66Combined sources8
Beta strandi69 – 79Combined sources11
Beta strandi90 – 98Combined sources9
Helixi100 – 102Combined sources3
Beta strandi104 – 106Combined sources3
Helixi107 – 121Combined sources15
Beta strandi126 – 132Combined sources7
Helixi136 – 144Combined sources9
Beta strandi148 – 159Combined sources12
Beta strandi165 – 174Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XNHX-ray2.10C1-176[»]
4XPDX-ray2.81C1-176[»]
4Y49X-ray3.95C/I/O1-176[»]
ProteinModelPortaliQ08689.
SMRiQ08689.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 176N-acetyltransferasePROSITE-ProRule annotationAdd BLAST163

Sequence similaritiesi

Belongs to the acetyltransferase family.Curated

Phylogenomic databases

HOGENOMiHOG000238056.
InParanoidiQ08689.
KOiK20793.
OMAiIVETKEH.
OrthoDBiEOG092C55DH.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRDICTLDN VYANNLGMLT KLAHVTVPNL YQDAFFSALF AEDSLVAKNK
60 70 80 90 100
KPSSKKDVHF TQMAYYSEIP VGGLVAKLVP KKQNELSLKG IQIEFLGVLP
110 120 130 140 150
NYRHKSIGSK LLKFAEDKCS ECHQHNVFVY LPAVDDLTKQ WFIAHGFEQV
160 170
GETVNNFIKG VNGDEQDAIL LKKHIS
Length:176
Mass (Da):19,727
Last modified:November 1, 1996 - v1
Checksum:i4F09DC597A690BA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75161 Genomic DNA. Translation: CAA99475.1.
AY557750 Genomic DNA. Translation: AAS56076.1.
BK006948 Genomic DNA. Translation: DAA11020.1.
PIRiS67150.
RefSeqiNP_014896.3. NM_001183672.3.

Genome annotation databases

EnsemblFungiiYOR253W; YOR253W; YOR253W.
GeneIDi854427.
KEGGisce:YOR253W.

Similar proteinsi

Entry informationi

Entry nameiNAT5_YEAST
AccessioniPrimary (citable) accession number: Q08689
Secondary accession number(s): D6W2V4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names